Testing Status of 2-Hydroxy-4-methoxybenzophenone 10260-S

 

CASRN: 131-57-7

Formula: C14-H12-O3

Synonyms/Common Names

  • Benzophenone 3
  • Escalol 567
  • HMB
  • (2-Hydroxy-4-methoxyphenyl)phenylmethanone
  • 4-Methoxy-2-hydroxybenzophenone
  • MOB
  • Oxybenzone
  • Sunscreen UV 15

Known Uses

Sunscreen agent. Photostabilizer for synthetic resins.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 2 weeks (Dosed-Feed)  (C60957A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 2 weeks (Topical Application)  (C60957B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 14-Day (Topical Application)  (C60957C)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13 weeks (Dosed-Feed)  (C60957A)  Report Complete 
    • TOX-21 (NIH Number: 92-3344)
      Toxicity Studies of 2-Hydroxy-4-methoxybenzophenone (CASRN 131-57-7) Administered Topically and in Dosed Feed to F344/N Rats and B6C3F1 Mice
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M:0, 3125, 6250, 12500, 25000, 50000PPM.
  • 13 weeks (Topical Application)  (C60957B)  Report Complete 
    • TOX-21 (NIH Number: 92-3344)
      Toxicity Studies of 2-Hydroxy-4-methoxybenzophenone (CASRN 131-57-7) Administered Topically and in Dosed Feed to F344/N Rats and B6C3F1 Mice
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R:0, 12.5, 25, 50, 100, 200; M:0, 22.75, 45.5, 91, 182, 364 MG/KG.

Long-Term Carcinogenicity

  • 2 years (Dosed-Feed)  (C10260)  Report Draft 
    • Rats: Harlan Sprague-Dawley; Mice: B6C3F1
    • Dose: R&M: 0, 1000, 3000, or 10000 ppm; R: 60/sex/dose @ 0 or 10000 and 50/sex/dose @ 1000 or 3000, M: 50/sex/dose.

Modified One-Generation

  • During gestation, beginning GD6, delivery and up to PND21 Dose Range Finding (Dosed-Feed)  (MOG002)  Completed 
    • Female Rats: Harlan Sprague-Dawley
  • Developmental Toxicity (Dosed-Feed)  (MOG002B)  Completed 
    • Rats: Harlan Sprague-Dawley
    • Dose: 0, 3000, 10000, or 30000 ppm.
  • F0 Generation (Dosed-Feed)  (MOG002B)  Completed 
    • Rats: Harlan Sprague-Dawley
    • Dose: 0, 3000, 10000, or 30000 ppm.
  • Fertility Assessment (Dosed-Feed)  (MOG002B)  Completed 
    • Rats: Harlan Sprague-Dawley
    • Dose: 0, 3000, 10000, or 30000 ppm.

Special Studies

  • Absorption Disposition Metabolism Elimination (Dermal; Gavage)  (K10260)  Completed 
    • Citation: Disposition and Metabolism of 2-Hydroxy-4-Methoxybenzophenone (CAS No. 131-57-7) in Harlan Sprague Dawley Rats and B6C3F1/N Mice after Oral Gavage, Intravenous Administration, and Dermal Application February 28, 2014
    • Male and Female Rats: Harlan Sprague-Dawley; Male and Female Mice: B6C3F1; Male and Female Rats: Sprague Dawley; Male and Female Mice: B6C3F1
    • Dose: 0.1, 10 mg/kg. 10, 100, 500 mg/kg.
  • Absorption Disposition Metabolism Elimination (Gavage)  (S0073)  Completed 
    • Citation: el Dareer SM, Kalin JR, Tillery KF, Hill DL. Disposition of 9-aminoacridine in rats dosed orally or intravenously and in monkeys dosed topically. J Toxicol Environ Health. 1985;15(6):789-99.Pubmed Abstract
    • Male Rats: F344/N
  • 3 days Hershberger Assay (Gavage)  (K10260C)  Completed 
    • Citation: Final Report: The Hershberger Bioassay; Oxybenzone, Octylmethoxycinnamate, Octylsalate, Octocrylene. 04 January 2012
    • Male Rats: Harlan Sprague-Dawley
    • Dose: 20, 64, or 200 mg/ml.
  • Toxicokinetic Study (Dosed-Feed; Gavage; Intravenous)  (S0593)  Completed 
    • Male and Female Rats: F344/N; Male and Female Mice: B6C3F1; Male and Female Rats: F344/N; Male and Female Mice: B6C3F1; Male and Female Rats: F344/N; Male and Female Mice: B6C3F1
    • Dose: Rats (iv) 8 mg/kg; (gavage) 8, 80 mg/kg; (feed) 1000, 10000 ppm Mice (iv) 50 mg/kg; (gavage) 50, 500 mg/kg; (feed) 1000, 10000 ppm.
  • 3 days Uterotrophic Assay (Gavage)  (K10260B)  Completed 
    • Citation: Final Report. The Uterotrophic Assay with Oxybenzone, Octylmethoxycinnamate, Octylsalate and Octocrylene. 01 September 2011 Revised: 20 April 2012
    • Female Rats: Sprague Dawley
    • Dose: 5 mL/kg.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (059393)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Micronucleus  (A48127)  Completed 
    • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (381877)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Weakly Positive 
  • Salmonella  (613844)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative 

Organ Systems Toxicity

  • GD 6 to GD 15 Developmental Toxicity (Dosed-Feed)  (R10260B)  Report Complete 
    • Male and Female Rats: Harlan Sprague-Dawley
    • Dose: 0, 3K, 10K or 30K ppm and 0.05 ppm ethinyl estradiol; 25/sex/dose.
  • GD 6 to GD 15 Developmental Toxicity (Dosed-Feed)  (R10260C)  Completed 
    • Male and Female Rats: Harlan Sprague-Dawley
    • Dose: 0, 3K, 10K or 30K ppm; 25 sex/dose.
  • GD 6 to PND 21 Developmental Toxicity (Dosed-Feed)  (R10260D)  Report in Preparation 
    • Male and Female Rats: Harlan Sprague-Dawley
    • Dose: 0, 3K, 10K or 30K ppm and 0.05 ppm ethinyl estradiol; 25/sex/dose.
  • 35 days Developmental Toxicity (Dosed-Feed)  (R10260)  Completed 
    • Rats: Harlan Sprague-Dawley
    • Dose: 0, 1K, 3K, 10K, 25K and 50K ppm.
  • Immunotoxicity  (I10260)  Assigned 
    • Male and Female Rats: Harlan Sprague-Dawley
  • greater than 120 days Continuous Breeding (Legacy) (Dosed-Feed)  (RACB88076)  Completed 
    • Citation: Final Report on the Reproductive Toxicity of 2-Hydroxy-4-methoxybenzophenone in CD-1 Swiss Mice
    • Male and Female Mice: Swiss
    • Dose: 0, 1.25, 2.5, 5%; 0, .3125, .625, 1.25, 2.5, 5%; 0, 5%; .

Toxicogenomics

  • Microarray Analysis Selected (T10260)  

    in vitro Testing

    • Androgen Receptor Binding Assay (Whole Body Exposure)  (V10260B)  Completed 
      • Androgen Receptor Binding Assay
      • Rats: Sprague Dawley
    • Estrogen Receptor Binding Assay (Whole Body Exposure)  (V10260C)  Completed 
      • Estrogen Receptor Binding Assay
      • Rats: Sprague Dawley
    • Androgen Receptor Transactivation Assay (Whole Body Exposure)  (V10260D)  Completed 
      • Androgen Receptor Transactivation Assay
    • Estrogen Receptor Transactivation Assay  (V10260E)  Completed 
      • Estrogen Receptor Transactivation Assay
    • Human Recombinant Aromatase Assay  (V10260G)  Completed 
      • Human Recombinant Aromatase Assay
    • H295R Steroidogenesis Assay (Whole Body Exposure)  (V10260)  Completed 
      • H295R Steroidogenesis Assay