National Toxicology Program

National Toxicology Program

Testing Status of Isobutyraldehyde - 10261-W


CASRN: 78-84-2

Formula: C4-H8-O

Synonyms/Common Names

  • 2-Methylpropanal (9CI)

Known Uses

Synthesis of pantothenic acid, cellulose esters, perfumes, flavors, and gasoline additives. Chemical intermediate. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Inhalation)  (C60968A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Inhalation)  (C60968A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0,500,8000 PPM/10 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Inhalation)  (C60968B)  Report Complete 
    • TR-472 (NIH Number: 99-3962)  (Peer Review Approval 12/12/1996A )
      Toxicology and Carcinogenesis Studies of Isobutyraldehyde (CASRN 78-84-2) in F344/N Rats and B6C3F1 Mice (Inhalation Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R&M: 0, 500, 1000, OR 2000 PPM (50/SEX/SPECIES/GROUP).

Genetic Toxicology

  • Chromosome Aberrations  (A27944)  Completed 
    • Mice
    •  Positive 
  • In Vitro Cytogenetics (CA/SCE)  (984070)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Drosophila  (112954)  Completed 
    • Citation: Woodruff, R.C., Mason, J.M., Valencia, R., and Zimmering, S. Chemical mutagenesis testing in Drosophila: V. Results of 53 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 677-702
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Mouse Lymphoma  (927534)  Completed 
    • Mice
    •  Positive 
  • Micronucleus  (A56966)  Completed 
    • Rats: Fischer 344
    • Male Negative 
  • Micronucleus  (A60444)  Completed 
    • Mice: B6C3F1
    • Male Negative 
  • Micronucleus  (A73413)  Completed 
    • Mice: B6C3F1
    • Male Negative 
  • Salmonella  (700142)  Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Negative 
  • Salmonella  (941876)  Completed 
    •  Equivocal 

Organ Systems Toxicity

  • Immunotoxicity (Topical Application)  (IMM87038)  Completed 
    • Female Mice: B6C3F1
    • Dose: SENSITIZATION: 0,3,10,30%; CHALLENGE: 30%; POSITIVE CONTROL: 0.5% DNFB..
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.