National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-10265-l

Testing Status of Methyleugenol - 10265-L

 

CASRN: 93-15-2

Formula: C11-H14-O2

Synonyms/Common Names

  • 1,2-Dimethoxy-4-(2-propenyl)benzene

Known Uses

Flavoring agent in foods and beverages; as a fragrance in cosmetics; as an insect attractant in eradication programs and as an anesthetic in rodents. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 5 days (Gavage)  (C10265)  Assigned 
    • 5-Day Transcriptomic Dose-Response Studies
    • Rats: Harlan Sprague-Dawley
    • Dose: 0, 4.625, 9.25, 18.5, 37, 75, 150, 300, or 600 mg/kg; N=4.
  • 2 weeks (Gavage)  (C60991)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 10000, 20000 AND 40000 PPM/ RATS: 10 MALE, 5 FEMALE/GROUP; MICE: 5/SEX/GROUP.
  • 13 weeks (Gavage)  (C60991)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0,10,30,100,300,1000 MG/KG PLUS SHAM GAVAGE GROUP.

Long-Term Carcinogenicity

  • 2 years (Gavage)  (C60991B)  Report Complete 
    • TR-491 (NIH Number: 00-3950)  (Peer Review Approval 10/30/1998A )
      Toxicology and Carcinogenesis Studies of Methyleugenol (CASRN 93-15-2) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Clear Evidence 
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: R&M: 0, 37, 75, OR 150 MG/KG; 50/SEX/SPECIES/GROUP.

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage; Intravenous)  (S0844)  Completed 
    • Citation: Final Report Chemical Disposition in Mammals. 03/15/98 - 06/30/99. The in vivo and in vitro Metabolism, Disposition, and Covalent Binding of Methyleugenol.
    • Male Rats: F344/N; Female Mice: B6C3F1; Male Rats: F344/N
  • Metabolism (in-vitro)  (S0658)  Completed 
    • Citation: Burkey JL, Sauer JM, McQueen CA, Sipes IG. Cytotoxicity and genotoxicity of methyleugenol and related congeners- a mechanism of activation for methyleugenol. Mutat Res. 2000 Sep 20;453(1):25-33.Pubmed Abstract
    • Male and Female Rats; Male and Female Mice; Male and Female Human (Cell Lines)
  • Metabolism (in-vitro)  (S0898)  Completed 
    • Citation: Final Report Chemical Disposition in Mammals. 03/15/98 - 06/30/99. The in vivo and in vitro Metabolism, Disposition, and Covalent Binding of Methyleugenol.
    • Male Rats: F344/N; Female Mice: B6C3F1; Human (Cell Lines)
  • Other (in-vitro)  (S0826)  Completed 
    • Citation: Burkey JL, Sauer JM, McQueen CA, Sipes IG. Cytotoxicity and genotoxicity of methyleugenol and related congeners- a mechanism of activation for methyleugenol. Mutat Res. 2000 Sep 20;453(1):25-33.Pubmed Abstract
    • Male Rats: Fischer 344; Mice: B6C3F1
  • Toxicokinetic Study (Gavage; Intravenous)  (K10265)  Completed 
    • Citation: Toxicokinetic Study Report Single Administration Toxicokinetic Study (Intravenous and Gavage Routes) of Methyleugenol in Fischer 344/N Rats and B6C3F1 Mice. February 23, 2001. Revised January 14, 2002.
    • Rats: Fischer 344; Mice: B6C3F1; Rats: F344/N; Mice: B6C3F1
  • Toxicokinetic Study (Gavage)  (S0577)  Completed 
    • Male and Female Rats: F344/N; Male and Female Mice: B6C3F1

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (614248)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Negative 
  • Micronucleus  (A29699)  Completed 
    • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (690169)  Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Negative 

Organ Systems Toxicity

  • 6 to 19 GD Conventional Teratology (Gavage)  (TER97007)  Completed 
    • Citation: DEVELOPMENTAL TOXICITY EVALUATION FOR METHYLEUGENOL (CASRN 93-15-2) ADMINISTERED BY GAVAGE TO SPRAGUE-DAWLEY (CD(r)) RATS ON GESTATIONAL DAYS 6 THROUGH 19 (Laboratory Supplement)
    • Citation: Developmental Toxicity Evaluation for Methyleugenol (CASRN 93-15-2) Administered by Gavage to Sprague-Dawley (CD®) Rats on Gestational Days 6 through 19
    • Female Rats: Sprague Dawley
    • Dose: 0, 80, 200, OR 500 MG/KG/DAY.
  • 6 to 15 GD Teratology Pilot Studies (Gavage)  (TRP92045)  Completed 
    • Female Rats: Sprague Dawley
    • Dose: 0, 300, 600, 800, 1000, OR 1200 MG/KG.
  • 6 to 19 GD Teratology Pilot Studies (Gavage)  (TRP92046)  Completed 
    • Female Rabbit: New Zealand White
    • Dose: 0, 100, 400, 600, 800, OR 1000 MG/KG.
  • 6 to 19 GD Teratology Pilot Studies (Gavage)  (TRP97007)  Completed 
    • Female Rats: Sprague Dawley
    • Dose: 0, 31.25, 62.5, 125, 250, OR 500 MG/KG/DAY.

Toxicogenomics

  • (T10265)  Assigned 
    NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.