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Testing Status of Chloroform 10384-E

 

CASRN: 67-66-3

Formula: C-H-Cl3

Synonyms/Common Names

  • Trichloromethane

Known Uses

Chemical intermediate for fluorocarbon 22, mildewcide for tobacco seedlings, soil fumigant & insecticide, dry cleaning agent, chemical intermediate for dyes (HSDB 1990)

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Gavage)  (C02686)  Completed 
    • Rats: Osborne Mendel; Mice: B6C3F1
  • 13-Week (Gavage)  (C02686)  Completed 
    • Rats: Osborne Mendel; Mice: B6C3F1

Long-Term Carcinogenicity

  • 2-Year (Gavage)  (C02686)  Completed 
    • TR-000 (67-66-3) (NIH Number: 76-1279)  (Peer Review Approval 09/00/1976A )
      Report on the Carcinogenesis Bioassay of Chloroform (CASRN 67-66-3)
    • Rats: Osborne Mendel; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Positive 
      • Female Rats Negative 
      • Male Mice Positive 
      • Female Mice Positive 
    • Dose: MR:0, 90, 180 FR:0, 125, 250 (WK 1-22) 0, 90, 180 (WK 23-SAC) FM:0, 7, 14 MM:0, 4, 8 MG/KG/50 PER GROUP.

Special Studies

  • Absorption Disposition Metabolism Elimination (Dosed-Water; Intraperitoneal Injection)  (S0059)  Completed 
    • Male Rats: F344/N

Genetic Toxicology

  • Chromosome Aberrations  (142011)  Completed 
    • Mice
    •  Negative 
  • In Vitro Cytogenetics (CA/SCE)  (564651)  Completed 
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Equivocal 
  • Mouse Lymphoma  (907612)  Completed 
    • Citation: Myhr, B.C., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for sixty-three coded chemicals tested at Litton Bionetics, Inc. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 103-194
    • Mice
  • Mouse Lymphoma  (994137)  Completed 
    • Citation: Mitchell, A.D., Rudd, C.J., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for 63 coded chemicals tested at SRI International. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 37-102
    • Mice
    •  Positive 
  • Micronucleus  (142011)  Completed 
    • Mice: B6C3F1
    • Male Positive 
  • Salmonella  (749633)  Completed 
    •  Negative 
  • Sister Chromatid Exchanges  (142011)  Completed 
    • Mice
    • Sister Chromatid  Negative 

Organ Systems Toxicity

  • 14 days Immunotoxicity (Gavage)  (IMM92050)  Completed 
    • Citation: Munson AE, Sain LE, Sanders VM, Kauffmann BM, White KL Jr., Page DG, Barnes DW, Borzelleca JF. (1982). Toxicology of Organic Water Contaminants: Trichloromethane, Bromodichloromethane, Dibromochloromethane and Tribromomethane. Environmental Health Perspectives, 46: 117-126.
    • Male and Female Mice: CD-1
    • Dose: 0, 50, 125, 250 MG/KG.
  • Continuous Breeding (Legacy) (Gavage)  (RACB87045)  Completed 
    • Citation: Chloroform Reproduction and Fertility Assessment in CD-1 Mice When Administered By Gavage
    • Male and Female Mice: Swiss
    • Dose: N/A; 0, 6.6, 15.9, 41.2 MG/KG; N/A; 0, 41.2 MG/KG.