National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-10396-v

Testing Status of Tribromomethane - 10396-V

 

CASRN: 75-25-2

Formula: C-H-Br3

Synonyms/Common Names

  • Bromoform
  • Methane, tribromo-

Known Uses

Synthesis of pharmaceuticals, in shipbuilding, aircraft industry, in fire-resistant chemicals, sedative medication, antitussive, antiseptic (HSDB 1990)

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 2 weeks (Gavage)  (C55130)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13 weeks (Gavage)  (C55130)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0, 50, 800 MG/KG/10 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Gavage)  (C55130)  Report Complete 
    • TR-350 (NIH Number: 89-2805)  (Peer Review Approval 04/18/1988A )
      Toxicology and Carcinogenesis Studies of Tribromomethane (Bromoform) (CASRN 75-25-2) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Some Evidence 
      • Female Rats Clear Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R&FM: 0, 100, 200, MM: 0, 50, 100 MG/KG/50 PER GROUP.

Genetic Toxicology

  • Chromosome Aberrations  (244282)  Completed 
    • Mice
    •  Negative 
  • In Vitro Cytogenetics (CA/SCE)  (845432)  Completed 
    • Citation: Anderson, B.E., Zeiger, E., Shelby, M.D., Resnick, M.A., Gulati, D.K., Ivett, J.L., and Loveday, K.S. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 55-137
    • Citation: Galloway, S., Bloom, A., Resnick, M., Margolin, B., Nakamura, F., Archer, P., and Zeiger, E. Development of a standard protocol for in vitro cytogenetic testing with CHO cells: Comparison of results for 22 compounds in two laboratories. Environ. Mutagen. Vol. 7 (1985) 1-52
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Negative 
  • In Vitro Cytogenetics (CA/SCE)  (973869)  Completed 
    • Citation: Anderson, B.E., Zeiger, E., Shelby, M.D., Resnick, M.A., Gulati, D.K., Ivett, J.L., and Loveday, K.S. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 55-137
    • Citation: Galloway, S., Bloom, A., Resnick, M., Margolin, B., Nakamura, F., Archer, P., and Zeiger, E. Development of a standard protocol for in vitro cytogenetic testing with CHO cells: Comparison of results for 22 compounds in two laboratories. Environ. Mutagen. Vol. 7 (1985) 1-52
    • Sister Chromatid Exchange Weakly Positive 
    • Chromosome Aberrations Negative 
  • Drosophila  (933572)  Completed 
    • Citation: Woodruff, R.C., Mason, J.M., Valencia, R., and Zimmering, S. Chemical mutagenesis testing in Drosophila: V. Results of 53 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 677-702
    • Sex-Linked Recessive Lethal Positive 
    • Reciprocal Translocation Negative 
  • Mouse Lymphoma  (701597)  Completed 
    • Citation: Myhr B.C., McGregor, D., Bowers, L., Riach, C., Brown, A., Edwards, I., McBride, D., Martin, R., and Caspary, W.J. L5178Y mouse lymphoma cell mutation assay results with 41 compounds. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 138-167
    • Mice
    •  Positive 
  • Micronucleus  (244282)  Completed 
    • Mice: B6C3F1
    • Male Negative 
  • Salmonella  (312147)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    • Citation: Zeiger, E. Mutagenicity of 42 chemicals in Salmonella Environ. Molec. Mutagen. Vol. 16(Suppl 18) (1990) 32-54
    •  Negative 
  • Salmonella  (445991)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    • Citation: Zeiger, E. Mutagenicity of 42 chemicals in Salmonella Environ. Molec. Mutagen. Vol. 16(Suppl 18) (1990) 32-54
    •  Equivocal 
  • Salmonella  (460994)  Completed 
    • Citation: Zeiger, E. Mutagenicity of 42 chemicals in Salmonella Environ. Molec. Mutagen. Vol. 16(Suppl 18) (1990) 32-54
    •  Positive 
  • Salmonella  (871929)  Completed 
    • Citation: Zeiger, E. Mutagenicity of 42 chemicals in Salmonella Environ. Molec. Mutagen. Vol. 16(Suppl 18) (1990) 32-54
    •  Equivocal 
  • Sister Chromatid Exchanges  (244282)  Completed 
    • Mice
    • Sister Chromatid  Positive 

Organ Systems Toxicity

  • 120 day minimum Continuous Breeding (Legacy) (Gavage)  (RACB87111)  Completed 
    • Citation: Bromoform Reproduction and Fertility Assessment in Swiss CD-1 Mice When Administered By Gavage
    • Male and Female Mice: Swiss
    • Dose: 0, 50, 100, 200, 400, 800 MG/KG; 0, 50, 100, 200 MG/KG; N/A; 0, 200 MG/KG.
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