Testing Status of Rotenone 10420-W

 

CASRN: 83-79-4

Formula: C23-H22-O6

Synonyms/Common Names

  • (2R-(2-alpha,6Aalpha,12Aalpha))-1,2,12,12A-tetrahydro-8,9-dimethoxy-2-(1-methylethenyl)-(1)benzopyrano(3,4-B)furo(2,3-H)(1)benzopyran-6(6aH)-one (9CI)

Known Uses

Fish poison, medical antiprotozoal, insecticide on fruits, livestock, vegetables, forage crops, occurs naturally in certain roots (Merck 1990). Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 2 weeks (Dosed-Feed)  (C55210B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13 weeks (Dosed-Feed)  (C55210B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 75, 150, 300, 600, OR 1200 PPM M: 0, 600, 1900, 5000, 16000, OR 50000 PPM.

Long-Term Carcinogenicity

  • 88 weeks (Intraperitoneal Injection)  (C55210A)  Report Complete 
    •   (Peer Review Approval NA) Citation: Tumorogenic Potential of Rotenone and its Specificity for Mammary Tissue, published by National Center for ToxicologicalResearch (NCTR Experiment No. 216). Call NCTR (501-543-7115) for availability of document.
    • Rats: F344/N
    • Dose: 986 & 1972 UG/KG - 5 DAYS/WK FOR 42 INJ. DAYS/72 PER GROUP.
  • 2 years (Dosed-Feed)  (C55210B)  Report Complete 
    • TR-320 (NIH Number: 88-2576)  (Peer Review Approval 08/19/1986A )
      Toxicology and Carcinogenesis Studies of Rotenone (CASRN 83-79-4) in F344/N Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Equivocal Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R: 0, 38, OR 75 PPM M: 0, 600, OR 1200 PPM; 50 PER GROUP.

Special Studies

  • Cell Proliferation (Dosed-Feed)  (S0590)  Completed 
    • Citation: Cunningham ML, Soliman MS, Badr MZ, Matthews HB. Rotenone, an anticarcinogen, inhibits cellular proliferation but not peroxisome proliferation in mouse liver. Cancer Lett. 1995 Aug 16;95(1-2):93-7.Pubmed Abstract
    • Mice: B6C3F1
    • Dose: 600PPM.
  • C. elegans  (W10420)  Completed 
    • C. elegans
    • Dose: 100, 75, 50 uM.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (195015)  Completed 
    • Citation: Anderson, B.E., Zeiger, E., Shelby, M.D., Resnick, M.A., Gulati, D.K., Ivett, J.L., and Loveday, K.S. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 55-137
    • Sister Chromatid Exchange Not a Valid Test 
    • Chromosome Aberrations Negative 
  • In Vitro Cytogenetics (CA/SCE)  (743095)  Completed 
    • Citation: Anderson, B.E., Zeiger, E., Shelby, M.D., Resnick, M.A., Gulati, D.K., Ivett, J.L., and Loveday, K.S. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 55-137
    • Sister Chromatid Exchange Weakly Positive 
    • Chromosome Aberrations Not Tested 
  • Mouse Lymphoma  (542524)  Completed 
    • Citation: McGregor, D., Brown, A., Cattanach, P., Edwards, I., McBride, D., Riach, C., and Caspary, W. Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay: III. 72 coded chemicals. Environ. Molec. Mutagen. Vol. 12 (1988) 85-154
    • Citation: Myhr B.C., McGregor, D., Bowers, L., Riach, C., Brown, A., Edwards, I., McBride, D., Martin, R., and Caspary, W.J. L5178Y mouse lymphoma cell mutation assay results with 41 compounds. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 138-167
    • Mice
    •  Positive 
  • Salmonella  (635953)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative