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Testing Status of Benzidine 10446-K


CASRN: 92-87-5

Formula: C12-H12-N2

Synonyms/Common Names

  • (1,1'-Biphenyl)-4,4'-diamine

Known Uses

Used in manufacturing dyes. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage)  (S0083)  Completed 
    • Citation: Lynn RK, Garvie-Gould CT, Milam DF, Scott KF, Eastman CL, Ilias AM, Rodgers RM. Disposition of the aromatic amine, benzidine, in the rat: characterization of mutagenic urinary and biliary metabolites. Toxicol Appl Pharmacol. 1984 Jan;72(1):1-14.Pubmed Abstract
    • Male Rats: F344/N
    • Dose: PO 5 MG/KG; IV 2.5 MG/KG.
  • Metabolism (in-vitro)  (S0438)  Completed 
    • Citation: Lynn RK, Garvie-Gould C, Milam DF, Scott KF, Eastman CL, Rodgers RM. Metabolism of the human carcinogen, benzidine, in the isolated perfused rat liver. Drug Metab Dispos. 1983 Mar-Apr;11(2):109-14.Pubmed Abstract
    • Male Rats: F344/N
  • Metabolism (Dosed-Feed)  (S0771)  Completed 
    • Citation: Luster MI, Tucker AN, Hayes HT, Pung OJ, Burka T, McMillan R, Eling T. Immunosuppressive effects of benzidine in mice: evidence of alterations in arachidonic acid metabolism. J Immunol. 1985 Oct;135(4):2754-61.Pubmed Abstract
    • Mice

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (993740)  Completed 
    • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Salmonella  (038268)  Completed 
    •  Positive 
  • Salmonella  (267901)  Completed 
    •  Positive 
  • Salmonella  (336867)  Completed 
    •  Positive 
  • Salmonella  (381549)  Completed 
    •  Positive 
  • Salmonella  (500249)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    •  Positive 
  • Salmonella  (503193)  Completed 
    •  Positive 
  • Salmonella Reduction  (609278)  Completed 
    • Citation: Reid, T., Morton K., and King, C. Mutagenicity of azo dyes following metabolism by different reductive/oxidative systems. Environ. Mutagen. Vol. 6 (1984) 705-717
    •  Positive 

Organ Systems Toxicity

  • Immunotoxicity  (IMM83004)  Completed 
    • Female Mice: B6C3F1