National Toxicology Program

National Toxicology Program

Testing Status of 2,4-Diaminotoluene (2,4-toluene diamine) - 10463-P


CASRN: 95-80-7


Formula: C7-H10-N2

Synonyms/Common Names

  • 4-Methyl-1,3-benzenediamine
  • 2,4-Toluenediamine

Known Uses

Intermediate for polyurethanes and dyes; in hair dyes (formerly)

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 13 weeks (Dosed-Feed)  (C02302)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 250, 3000, M: 0, 100, 1000 PPM/5 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Dosed-Feed)  (C02302)  Report Complete 
    • TR-162 (NIH Number: 79-1718)  (Peer Review Approval 12/13/1978A )
      Bioassay of 2,4-Diaminotoluene for Possible Carcinogenicity (CASRN 95-80-7)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Positive 
      • Female Rats Positive 
      • Male Mice Negative 
      • Female Mice Positive 
    • Dose: MR: 0, 79, 176 (AVG), FR: 0, 79, 171 (AVG), M: 0, 100, 200 PPM/50 PER GROUP.

Special Studies

  • Cell Proliferation (Gavage)  (S0696)  Completed 
    • Citation: Cunningham ML, Foley J, Maronpot RR, Matthews HB. Correlation of hepatocellular proliferation with hepatocarcinogenicity induced by the mutagenic noncarcinogen:carcinogen pair - 2,6- and 2,4-diaminotoluene. Toxicol Appl Pharmacol. 1991 Mar 1;107(3):562-7.Pubmed Abstract
    • Male Rats: F344/N
  • Mechanisms (Intraperitoneal Injection)  (S0697)  Completed 
    • Citation: Cunningham ML, Matthews HB. Evidence for an acetoxyarylamine as the ultimate mutagenic reactive intermediate of the carcinogenic aromatic amine 2,4-diaminotoluene. Mutat Res. 1990 Oct;242(2):101-10.Pubmed Abstract
    • Male Rats: F344/N
  • Mechanisms (Gavage)  (S0698)  Completed 
    • Citation: Cunningham ML, Burka LT, Matthews HB. The interaction of methanol, rat-liver S9 and the aromatic amine 2,4-diaminotoluene produces a new mutagenic compound. Mutat Res. 1990 Aug;244(4):273-7.Pubmed Abstract
    • Male Rats: F344/N
  • Metabolism (Gavage)  (S0699)  Completed 
    • Citation: Cunningham ML, Matthews HB, Burka LT. Activation of methanol by hepatic postmitochondrial supernatant: formation of a condensation product with 2,4-diaminotoluene. Chem Res Toxicol. 1990 Mar-Apr;3(2):157-61. Pubmed Abstract
    • Male Rats: F344/N
  • Metabolism (Dosed-Feed)  (S0700)  Completed 
    • Citation: Furlong BB, Weaver RP, Goldstein JA. Covalent binding to DNA and mutagenicity of 2,4-diaminotoluene metabolites produced by isolated hepatocytes and 9000 g supernatant from Fischer 344 rats. Carcinogenesis. 1987 Feb;8(2):247-51.Pubmed Abstract
    • Male Rats: F344/N
  • Other (Intraperitoneal Injection)  (S0701)  Completed 
    • Citation: Hayward JJ, Shane BS, Tindall KR, Cunningham ML. Differential in vivo mutagenicity of the carcinogen/non-carcinogen pair 2,4- and 2,6-diaminotoluene. Carcinogenesis. 1995 Oct;16(10):2429-33.Pubmed Abstract
    • Male Mice: B6C3F1

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (314882)  Completed 
    • Citation: Loveday, K.S., Anderson, B.E., Resnick, M.A., and Zeiger, E. Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro V: Results with 46 chemicals Environ. Molec. Mutagen. Vol. 16 (1990) 272-303
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Micronucleus  (520516)  Completed 
    • Mice: B6C3F1
    • Male Negative 
  • Micronucleus  (571690)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (707834)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    •  Positive 

Organ Systems Toxicity

  • 14 days Immunotoxicity (Gavage)  (IMM87034)  Completed 
    • Female Mice: B6C3F1
    • Dose: 0, 25, 50, 100 MG/KG.
  • Immunotoxicity (Topical Application)  (IMM92040)  Completed 
    • Female Mice: B6C3F1
    • Dose: 0, 1, 3, 10%.
  • 8 consecutive days (MTD PHASE), 7 to 14 GD (reproductive phase) Short-Term in vivo Repro. Tox. (Gavage)  (T0039)  Completed 
    • Citation: Determination of the Reproductive Effects in Mice of Nine Selected Chemicals
    • Female Mice: Swiss
    • Dose: 150 MG/KG BODY WT..
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.