National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-10468-j

Testing Status of Eugenol - 10468-J

 

CASRN: 97-53-0

Formula: C10-H12-O2

Synonyms/Common Names

  • 2-Methoxy-4-(2-propenyl)-phenol (9CI)

Known Uses

Flavoring agent & fragrance, food additive, obtained from oil of cloves, fungicide, insect attractant (Merck 1989). Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Dosed-Feed)  (C50453)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Dosed-Feed)  (C50453)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 800, 12500, M: 0, 400, 6000 PPM/10 PER GROUP.

Long-Term Carcinogenicity

  • 2-Year (Dosed-Feed)  (C50453)  Report Complete 
    • TR-223 (NIH Number: 83-1779)  (Peer Review Approval 02/18/1981A )
      Carcinogenesis Studies of Eugenol (CASRN 97-53-0) in F344/N Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Negative 
      • Female Rats Negative 
      • Male Mice Equivocal 
      • Female Mice Equivocal 
    • Dose: FR: 0, 6000, 12500, MR&M: 0, 3000, 6000 PPM/50 PER GROUP.

Special Studies

  • Metabolism (in-vitro)  (S0656)  Completed 
    • Citation: Burkey JL, Sauer JM, McQueen CA, Sipes IG. Cytotoxicity and genotoxicity of methyleugenol and related congeners- a mechanism of activation for methyleugenol. Mutat Res. 2000 Sep 20;453(1):25-33.Pubmed Abstract
    • Rats; Mice; Human (Cell Lines)

Genetic Toxicology

  • Chromosome Aberrations  (635999)  Completed 
    • Mice
    •  Equivocal 
  • In Vitro Cytogenetics (CA/SCE)  (416109)  Completed 
    • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Drosophila  (742892)  Completed 
    • Citation: Foureman, P., Mason, J.M., Valencia, R., Zimmering, S. Chemical Mutagenesis Testing in Drosophila. IX. Results of 50 coded compounds tested for the National Toxicology Program. Environ. Molec. Mutagen. Vol. 23 (1994) 51-63
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Mouse Lymphoma  (134323)  Completed 
    • Citation: Myhr, B. and Caspary, W. Chemical mutagenesis at the TK locus in L5178Y mouse lymphoma cells. I. Results for 31 coded compounds in the National Toxicology Program. Environ. Molec. Mutagen. Vol. 18 (1991) 51-83
    • Mice
    •  Positive 
  • Micronucleus  (210426)  Completed 
    • Citation: Shelby, M.D., Erexson, G.L., Hook, G.J., and Tice, R.R. Evaluation of a three-exposure mouse bone marrow micronucleus protocol: Results with 49 chemicals. Environ. Molec. Mutag. Vol. 21 (1993) 160-179
    • Mice: B6C3F1
    • Male Negative 
  • Salmonella  (692593)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    •  Negative 
  • Sister Chromatid Exchanges  (635999)  Completed 
    • Mice
    • Sister Chromatid  Positive (Nonstandard Protocol) 

Organ Systems Toxicity

  • 6 to 15 GD Teratology Pilot Studies (Gavage)  (TRP92043)  Completed 
    • Female Rats: Sprague Dawley
    • Dose: 0, 100, 400, 600, 800, OR 1000 MG/KG.
  • Teratology Pilot Studies (Gavage)  (TRP92044)  Completed 
    • Rabbit: New Zealand White
    • Dose: 0, 50, 200, 400, 600, 800 MG/KG/DAY.
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