National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-10480-t

Testing Status of 4-Vinylcyclohexene - 10480-T

 

CASRN: 100-40-3

Formula: C8-H12

Synonyms/Common Names

  • 4-Ethenylcyclohexene (9CI)

Known Uses

Manufacture of polymers and epoxy resins. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Gavage)  (C54999)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Gavage)  (C54999)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 50, 800, M: 0, 75, 1200 MG/KG/10 PER GROUP.

Long-Term Carcinogenicity

  • 2-Year (Gavage)  (C54999)  Report Complete 
    • TR-303 (NIH Number: 86-2559)  (Peer Review Approval 03/29/1985A )
      Toxicology and Carcinogenesis Studies of 4-Vinylcyclohexene (CASRN 100-40-3) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Inadequate Study 
      • Female Rats Inadequate Study 
      • Male Mice Inadequate Study 
      • Female Mice Clear Evidence 
    • Dose: 0, 200, 400 MG/KG/50 PER GROUP.

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage)  (S0084)  Completed 
    • Citation: Smith BJ, Carter DE, Sipes IG. Comparison of the disposition and in vitro metabolism of 4-vinylcyclohexene in the female mouse and rat. Toxicol Appl Pharmacol. 1990 Sep 15;105(3):364-71.Pubmed Abstract
    • Citation: Smith BJ, Mattison DR, Sipes IG. The role of epoxidation in 4-vinylcyclohexene-induced ovarian toxicity. Toxicol Appl Pharmacol. 1990 Sep 15;105(3):372-81. Pubmed Abstract
    • Male Rats: F344/N; Male Mice: B6C3F1
    • Dose: M: 5 MG/ML/MOUSE R: 50 MG/ML/RAT.
  • Metabolism (Intraperitoneal Injection)  (S0800)  Completed 
    • Citation: Doerr JK, Hooser SB, Smith BJ, Sipes IG. Ovarian toxicity of 4-vinylcyclohexene and related olefins in B6C3F1 mice: role of diepoxides. Chem Res Toxicol. 1995 Oct-Nov;8(7):963-9.Pubmed Abstract
    • Female Mice: B6C3F1
    • Dose: Structure-Activity Studies: at either a dose equimolar to that of VCH (7.5 mmol/kg), or at a lower dose which had been determined fromprevious studies as the maximum tolerated dose by mice over 30 day period w/o significant weight loss. In Vivo Metabolism Studies: The mice were administered a single dose (7.5 mmol/kg), ip, of test com-pound in sesame seed oil..
  • Metabolism (Intraperitoneal Injection)  (S0801)  Completed 
    • Citation: Doerr-Stevens JK, Liu J, Stevens GJ, Kraner JC, Fontaine SM, Halpert JR, Sipes IG. Induction of cytochrome P-450 enzymes after repeated exposure to 4-vinylcyclohexene in B6C3F1 mice. Drug Metab Dispos. 1999 Feb;27(2):281-7.Pubmed Abstract
    • Female Mice: B6C3F1
    • Dose: VCH (7.5 mmol/ky/day x 10 days i.p.), sesame seed oil (vehicle control, 2.5 ml/kg/day x 10 days i.p.), PB (80 mg/kg/day x 5 days i.p.), DEX (100 mg/kg/day x 3 days i.p.), or acetone (ACE) [in drinking water 1 % (v/v) x 5 days]. PB was made up as 3.2% solution (w/v) in 0.9% NaCl, and DEX was in a 2% Tween 80 solution (v/v)..

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (169960)  Completed 
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Negative 
  • Mouse Lymphoma  (971117)  Completed 
    • Mice
    •  Positive 
  • Salmonella  (609542)  Completed 
    •  Negative 
  • Salmonella  (777152)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative 

Organ Systems Toxicity

  • 27 weeks Continuous Breeding (Legacy) (Gavage)  (RACB90035)  Completed 
    • Citation: Final Report on the Reproductive Toxicity of 4 Vinylcyclohexene in CD-1 Swiss Mice
    • Male and Female Mice: Swiss
    • Dose: N/A; 0, 100, 250, 500 MG/KG/DAY; N/A; 0, 500 MG/KG/DAY; .
  • single Total Reproductive Capacity (Intraperitoneal Injection)  (TRC98044)  Completed 
    • Female Mice: (SEC/E X C57BL/E)F1
    • Dose: 2000 MG/KG.
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.