National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-10499-l

Testing Status of Azobenzene - 10499-L

 

CASRN: 103-33-3

Formula: C12-H10-N2

Synonyms/Common Names

  • Diphenyldiazene

Known Uses

Intermediate for azo dyes and other organic chemicals, acaricide (Merck 1989). Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 13 weeks (Dosed-Feed)  (C02926)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0, 500, 4600 PPM/5 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Dosed-Feed)  (C02926)  Report Complete 
    • TR-154 (NIH Number: 79-1710)  (Peer Review Approval 12/13/1978A )
      Bioassay of Azobenzene for Possible Carcinogenicity (CASRN 103-33-3)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Positive 
      • Female Rats Positive 
      • Male Mice Negative 
      • Female Mice Negative 
    • Dose: R&MM: 0, 200, 400, FM: 0, 208, 545 (AVG) PPM/50 PER GROUP.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (657475)  Completed 
    • Citation: Galloway, S., Bloom, A., Resnick, M., Margolin, B., Nakamura, F., Archer, P., and Zeiger, E. Development of a standard protocol for in vitro cytogenetic testing with CHO cells: Comparison of results for 22 compounds in two laboratories. Environ. Mutagen. Vol. 7 (1985) 1-52
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Negative 
  • In Vitro Cytogenetics (CA/SCE)  (758475)  Completed 
    • Citation: Galloway, S., Bloom, A., Resnick, M., Margolin, B., Nakamura, F., Archer, P., and Zeiger, E. Development of a standard protocol for in vitro cytogenetic testing with CHO cells: Comparison of results for 22 compounds in two laboratories. Environ. Mutagen. Vol. 7 (1985) 1-52
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Equivocal 
  • Drosophila  (165449)  Completed 
    • Citation: Zimmering, S., Mason, J.M., Valencia, R., and Woodruff, R.C. Chemical mutagenesis testing in Drosophila. II. Results of 20 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 87-100
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Salmonella  (218794)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    •  Positive 
  • Salmonella  (513062)  Completed 
    •  Positive 
  • Salmonella  (514794)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    •  Positive 
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