National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-10514-j

Testing Status of 1,3-Butadiene - 10514-J

 

CASRN: 106-99-0

Formula: C4-H6

Synonyms/Common Names

  • alpha,gamma-Butadiene

Known Uses

Chemical intermediate. Manufacture of synthetic rubber and of thermoplastic resins. Produced from petroleum gases (Merck 1989). Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 13 weeks (Inhalation)  (C50602B)  Completed 
    • Rats: F344/N
    • Dose: R: 0, 200, 625, 2000, OR 6250 PPM M: 0, 625, 1250, 5000, 8000, OR 16000 PPM.

Long-Term Carcinogenicity

  • 2 years (Inhalation)  (C50602A)  Report Complete 
    • TR-288 (NIH Number: 84-2544)  (Peer Review Approval 10/28/1983A )
      Toxicology and Carcinogenesis Studies of 1,3-Butadiene (CASRN 106-99-0) in B6C3F1 Mice (Inhalation Studies)
    • Mice: B6C3F1
    • Carcinogenesis Results
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: 0,625,1250 PPM/50 PER GROUP.
  • 2 years (Inhalation)  (C50602C)  Report Complete 
    • TR-434 (NIH Number: 92-3165)  (Peer Review Approval 11/21/1991A )
      Toxicology and Carcinogenesis Studies of 1,3-Butadiene (CASRN 106-99-0) in B6C3F1 Mice (Inhalation Studies)
    • Mice: B6C3F1
    • Carcinogenesis Results
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: 0, 6.25, 20, 62.5, 200, 625 PPM/50 PER GROUP.
  • 2 years (Inhalation)  (C50602D)  Histopath in progress 
    • Citation: Bucher JR, Melnick RL, and Hildebrandt PK. Lack of Carcinogenicity in Mice Exposed Once to High Concentrations of 1,3-Butadiene. J. Nat. Cancer Inst 85: 1866-1867 (1993).
    • Mice: B6C3F1
    • Dose: Mice only: 0, 1000, 5000, 10000 ppm/60 per group.

Special Studies

  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0220)  Completed 
    • Citation: Dahl AR, Bechtold WE, Bond JA, Henderson RF, Mauderly JL, Muggenburg BA, Sun JD, Birnbaum LS. Species differences in the metabolism and disposition of inhaled 1,3-butadiene and isoprene. Environ Health Perspect. 1990 Jun;86:65-9.Pubmed Abstract
    • Rats; Mice; Monkey
  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0792)  Completed 
    • Citation: Bond JA, Dahl AR, Henderson RF, Dutcher JS, Mauderly JL, Birnbaum LS. Species differences in the disposition of inhaled butadiene. Toxicol Appl Pharmacol. 1986 Jul;84(3):617-27.Pubmed Abstract
    • Male Rats: Sprague Dawley; Male Mice: B6C3F1
  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0793)  Completed 
    • Citation: Bond JA, Martin OS, Birnbaum LS, Dahl AR, Melnick RL, Henderson RF. Metabolism of 1,3-butadiene by lung and liver microsomes of rats and mice repeatedly exposed by inhalation to 1,3-butadiene. Toxicol Lett. 1988 Nov;44(1-2):143-51.Pubmed Abstract
    • Male Rats: Sprague Dawley; Male Mice: B6C3F1
  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0794)  Completed 
    • Citation: Bond JA, Dahl AR, Henderson RF, Birnbaum LS. Species differences in the distribution of inhaled butadiene in tissues. Am Ind Hyg Assoc J. 1987 Oct;48(10):867-72.Pubmed Abstract
    • Male Rats: Sprague Dawley; Male Mice: B6C3F1
  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0795)  Completed 
    • Citation: Sabourin PJ, Burka LT, Bechtold WE, Dahl AR, Hoover MD, Chang IY, Henderson RF. Species differences in urinary butadiene metabolites; identification of 1,2-dihydroxy-4-(N-acetylcysteinyl)butane, a novel metabolite of butadiene. Carcinogenesis. 1992 Sep;13(9):1633-8.Pubmed Abstract
    • Rats: F344/N; Rats: Sprague Dawley; Mice: B6C3F1; Hamsters: Syrian Golden; Monkey
  • Other (Intraperitoneal Injection)  (S0459)  Completed 
    • Citation: Sun JD, Dahl AR, Bond JA, Birnbaum LS, Henderson RF. Characterization of hemoglobin adduct formation in mice and rats after administration of [14C]butadiene or [14C]isoprene. Toxicol Appl Pharmacol. 1989 Aug;100(1):86-95.Pubmed Abstract
    • Male Rats: Sprague Dawley; Male Mice: B6C3F1
  • Toxicokinetic Study (Inhalation)  (S0796)  Completed 
    • Citation: Dahl AR, Sun JD, Birnbaum LS, Bond JA, Griffith WC Jr, Mauderly JL, Muggenburg BA, Sabourin PJ, Henderson RF. Toxicokinetics of inhaled 1,3-butadiene in monkeys: comparison to toxicokinetics in rats and mice. Toxicol Appl Pharmacol. 1991 Aug;110(1):9-19. Erratum in Toxicol Appl Pharmacol 1992 Sep;116(1):152.Pubmed Abstract
    • Rats; Mice; Monkey

Genetic Toxicology

  • Chromosome Aberrations  (A74454)  Completed 
    • Citation: Tice, R.R., Boucher, R., Luke, C.A., and Shelby, M.D. Comparative cytogenetic analysis of bone marrow damage induced in male B6C3F1 mice by multiple exposures to gaseous 1,3-butadiene. Environ. Mutagen Vol. 9 (1987) 235-250
    • Mice
  • Chromosome Aberrations  (A93093)  Completed 
    • Mice
    •  Positive 
  • Drosophila  (950073)  Completed 
    • Citation: Foureman, P., Mason, J.M., Valencia, R., Zimmering, S. Chemical Mutagenesis Testing in Drosophila. IX. Results of 50 coded compounds tested for the National Toxicology Program. Environ. Molec. Mutagen. Vol. 23 (1994) 51-63
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Mouse Lymphoma  (614566)  Completed 
    • Citation: McGregor, D., Brown, A.G., Cattanach, P., Edwards, I., McBride, D., Riach, C., Shepherd, W., and Caspary, W. Responses of the L5178Y mouse lymphoma forward mutation assay: V. Gases and vapors. Environ. Molec. Mutagen. Vol. 17 (1991) 122-129
    • Mice
    •  Negative 
  • Micronucleus  (A74454)  Completed 
    • Citation: Tice, R.R., Boucher, R., Luke, C.A., and Shelby, M.D. Comparative cytogenetic analysis of bone marrow damage induced in male B6C3F1 mice by multiple exposures to gaseous 1,3-butadiene. Environ. Mutagen Vol. 9 (1987) 235-250
    • Mice: B6C3F1
    • Male Positive (Nonstandard Protocol) 
  • Salmonella  (256166)  Completed 
    •  Positive 
  • Sister Chromatid Exchanges  (A74454)  Completed 
    • Citation: Tice, R.R., Boucher, R., Luke, C.A., and Shelby, M.D. Comparative cytogenetic analysis of bone marrow damage induced in male B6C3F1 mice by multiple exposures to gaseous 1,3-butadiene. Environ. Mutagen Vol. 9 (1987) 235-250
    • Mice

Organ Systems Toxicity

  • 5 consecutive days Dominant Lethal (Inhalation)  (T0183A)  Completed 
    • Citation: Hackett PL, Mast TJ, Brown MG, Clark ML, Evanoff, JJ. Dominant Lethal Study in CD-1 Mice Following Inhalation Exposure to 1,3-Butadiene: Final Technical Report. April 1988
    • Male Mice: Swiss
    • Dose: 200, 1000, or 5000 ppm.
  • 6 to 15 GD Conventional Teratology (Inhalation)  (TER85144)  Completed 
    • Citation: Inhalation Developmental Toxicology Studies: Teratology Study of 1,3-Butadiene in Mice (November 1987)
    • Female Mice: Swiss
    • Mice Maternal Positive 
    • Mice Fetal Positive 
    • Dose: 0, 40, 200, 1000 PPM.
  • 6 to 19 GD Conventional Teratology (Inhalation)  (TER89041)  Completed 
    • Citation: Inhalation Developmental Toxicology Studies of 1,3-Butadiene in the Rat (November 1987)
    • Female Rats: Sprague Dawley
    • Rats Maternal Negative 
    • Rats Fetal Negative 
    • Dose: 0, 40, 200, 1000 PPM.
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