National Toxicology Program

National Toxicology Program

Testing Status of C.I. Vat Yellow 4 - 10576-P


CASRN: 128-66-5

Formula: C24-H12-O2

Synonyms/Common Names

  • Dibenzo(b,def)chrysene-7,14-dione
  • Yellow 4, C.I. Vat

Known Uses

Dye for cellulose fibers and synthetics, wool, silk; pigment for paper; military smoke dye

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 13 weeks (Dosed-Feed)  (C03565)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0,6200,50000 PPM/5 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Dosed-Feed)  (C03565)  Report Complete 
    • TR-134 (NIH Number: 79-1389)  (Peer Review Approval 08/31/1978A )
      Bioassay of C.I. Vat Yellow 4 for Possible Carcinogenicity (CASRN 128-66-5)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Negative 
      • Female Rats Negative 
      • Male Mice Positive 
      • Female Mice Negative 
    • Dose: R: 0,3500,7000, MM: 0,25000,50000, FM: 0,12500,25000 PPM/50 PER GROUP.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (876338)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Negative 
  • Salmonella  (350442)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative 
  • Salmonella  (897779)  Completed 
    •  Negative 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.