National Toxicology Program

National Toxicology Program

Testing Status of C.I. Acid Orange 3 - 10737-X


CASRN: 6373-74-6

Formula: C18-H14-N4-O7-S.Na

Synonyms/Common Names

  • 5-((2,4-Dinitrophenyl)amino)-2-(phenylamino)benzenesulfonic acid monosodium salt
  • Orange 3, C.I. Acid

Known Uses

Dyeing acetate, wool, nylon, silk, and leather; shading component in soaps and wood stains. A dinitrodiphenylamine derivative used in semipermanent hair coloring products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Gavage)  (C54911)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Gavage)  (C54911)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0,94,1500, M: 0,250,2000 MG/KG/10 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Gavage)  (C54911)  Report Complete 
    • TR-335 (NIH Number: 89-2591)  (Peer Review Approval 07/14/1987A )
      Toxicology and Carcinogenesis Studies of C.I. Acid Orange 3 (CASRN 6373-74-6) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats Clear Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R: 0,375,750 MG/KG MM: 0,125,250 MG/KG FM: 0,250,500 MG/KG/50 PER GROUP.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (835697)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Salmonella  (537306)  Completed 
    • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelmans, K. Salmonella mutagenicity tests. IV. Results from the testing of 300 chemicals Environ. Molec. Mutagen. Vol. 11 (Suppl 12) (1988) 1-158
    •  Positive 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.