Skip to Main Navigation
Skip to Page Content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Testing Status of 3,3'-Dimethoxybenzidine dihydrochloride 10776-A

 

CASRN: 20325-40-0

Formula: C14-H16-N2-O2.2Cl-H

Synonyms/Common Names

  • 3,3'-Dimethoxy-(1,1-biphenyl)-4,4'-diamine dihydrochloride

Known Uses

Production of dyes

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Dosed-Water)  (C99989)  Completed 
    • Rats: F344/N
  • 13-Week (Dosed-Water)  (C99989)  Completed 
    • Rats: F344/N
    • Dose: 0, 170, 330, 630, 1250, 2500 PPM (10 PER GROUP).

Long-Term Carcinogenicity

  • 2 years (Dosed-Water)  (C99989)  Completed 
    • TR-372 (NIH Number: 89-2827)  (Peer Review Approval 06/27/1989A )
      Toxicology and Carcinogenesis Studies of 3,3'-Dimethoxybenzidine Dihydrochloride (CASRN 20325-40-0) in F344/N Rats (Drinking Water Studies)
    • Rats: F344/N
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Clear Evidence 
    • Dose: R: 0, 80, 170, 330 PPM /70, 45, 75, 70 PER GROUP RESPECTIVELY.

Special Studies

  • Metabolism (Dosed-Feed)  (S0017)  Completed 
    • Citation: Lynn RK, Donielson DW, Ilias AM, Kennish JM, Wong K, Matthews HB. Metabolism of bisazobiphenyl dyes derived from benzidine, 3,3'-dimethylbenzidine or 3,3'-dimethoxybenzidine to carcinogenic aromatic amines in the dog and rat. Toxicol Appl Pharmacol. 1980 Nov;56(2):248-58.Pubmed Abstract
    • Rats; Dog

Genetic Toxicology

  • Mouse Lymphoma  (640872)  Completed 
    • Citation: Mitchell, A.D., Rudd, C.J., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for 63 coded chemicals tested at SRI International. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 37-102
    • Mice
  • Mouse Lymphoma  (948661)  Completed 
    • Citation: Myhr, B.C., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for sixty-three coded chemicals tested at Litton Bionetics, Inc. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 103-194
    • Mice