Testing Status of Chlorambucil 10850-R

 

CASRN: 305-03-3

Formula: C14-H19-Cl2-N-O2

Synonyms/Common Names

  • 4-(Bis(2-chloroethyl)amino)benzenebutanoic acid

Known Uses

Chemotherapeutic agent used in the treatment of leukemia, lymphoma and Hodgkins

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Intraperitoneal Injection)  (C03485)  Completed 
    • Rats: Sprague Dawley; Mice: Swiss
  • 13-Week (Intraperitoneal Injection)  (C03485)  Completed 
    • Rats: Sprague Dawley; Mice: Swiss

Long-Term Carcinogenicity

  • 2-Year (Intraperitoneal Injection)  (C03485)  Report Complete 
    •   (Peer Review Approval NA) Citation: EK Weisburger, Bioassay Program for Carcinogenic Hazards of Cancer Chemotherapeutic Agents. Cancer 40:1935-1949 (1977)
    • Rats: Sprague Dawley; Mice: Swiss
    • Dose: R: 0, .24, .48, M: 0, .2, .4 MG/KG/25 PER GROUP.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (586147)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Drosophila  (253055)  Completed 
    • Citation: Woodruff, R.C., Mason, J.M., Valencia, R., and Zimmering, S. Chemical mutagenesis testing in Drosophila: V. Results of 53 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 677-702
    • Sex-Linked Recessive Lethal Positive 
    • Reciprocal Translocation Positive 
  • Micronucleus  (A05195)  Completed 
    • Mice: B6C3F1
    • Male Positive 
  • Salmonella  (724003)  Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Positive 

Organ Systems Toxicity

  • Dominant Lethal (Male) (Dosed-Feed)  (DLM96002)  Completed 
    • Citation: Generoso, WM, Witt, KL, Cain, KT, Hughes, L, Cacheiro, NLA, Lockhart, A-MC, Shelby, MD. Dominant lethal and heritable translocation tests with chlorambucil and melphalan in male mice. Mutat. Res. 345: 167-180. (1995)
    • Male Mice: C3H X 101
    • Mice Male Positive 
  • Heritable Translocation Test (Dosed-Feed)  (HTT96001)  Completed 
    • Citation: Generoso, WM, Witt, KL, Cain, KT, Hughes, L, Cacheiro, NLA, Lockhart, A-MC, Shelby, MD. Dominant lethal and heritable translocation tests with chlorambucil and melphalan in male mice. Mutat. Res. 345: 167-180. (1995)
    • Male Mice: C3H X 101
    • Mice Male Positive 
  • Morphological Specific Locus (Dosed-Feed)  (MSLM65)  Completed 
    • Citation: Russell, L.B., Hunsicker, P.R., Cacheiro, N.L.A., Bangham, J.W., Russell, W.L., and Shelby, M.D. Chlorambucil effectively induces deletion mutations in mouse germ cells, Proc. Natl. Acad. Sci. (USA), 86:3704-3708 (1989).
    • Male Mice: 101 X C3H
    • Mice Male Positive 
  • Morphological Specific Locus  (MSLT96001)  Completed 
    • Citation: Russell, LB, Hunsicker, PR, and Shelby, MD. Chlorambucil and bleomycin induce mutations in the specific-locus test in female mice. Mutation Res. 358: 25-35 (1996).
    • Female Mice: C3H X 101
    • Mice Female Positive 
  • Total Reproductive Capacity (Dosed-Feed)  (TRC98009)  Completed 
    • Citation: Bishop, JB, Morris, RW, Seely, JC, Hughes, LA, Cain, KT, Generoso, WM. Alterations in the Reproductive Patterns of Female Mice Exposed to Xenobiotics. Fund. and Appl. Tox. 40: 191-204 (1997).
    • Female Mice
    • Mice Female Positive