Testing Status of Isobutyl nitrite 10869-J

 

CASRN: 542-56-3

Formula: C4-H9-N-O2

Synonyms/Common Names

  • Isobutyl nitrite (AIDS)
  • Nitrous acid, 2-methylpropyl ester (9CI)

Known Uses

Industrial intermediate in chemical synthesis. It is also an ingredient of various incenses or room odorizers and is used as a euphoric. The chemical has also been used as a jet propellant and in the preparation of fuels.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 2 weeks (Inhalation)  (C61052B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13 weeks (Inhalation)  (C61052B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 10, 25, 75, 150, OR 300 PPM; 6 HR/DAY, 5 DAY/WEEK, 10/GROUP.

Long-Term Carcinogenicity

  • 2 years (Inhalation)  (C61052B)  Report Complete 
    • TR-448 (NIH Number: 96-3364)  (Peer Review Approval 11/29/1994A )
      Toxicology and Carcinogenesis Studies of Isobutyl Nitrite (CASRN 542-56-3) in F344 Rats and B6C3F1 Mice (Inhalation Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Clear Evidence 
      • Male Mice Some Evidence 
      • Female Mice Some Evidence 
    • Dose: R&M: 0, 37, 75, OR 150 PPM.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (016060)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • In Vitro Cytogenetics (CA/SCE)  (841266)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Drosophila  (399025)  Completed 
    • Citation: Woodruff, R.C., Mason, J.M., Valencia, R., and Zimmering, S. Chemical mutagenesis testing in Drosophila: V. Results of 53 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 677-702
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Micronucleus  (A87798)  Completed 
    • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
    • Mice: B6C3F1
    • Male Positive 
    • Female Positive 
  • Salmonella  (234350)  Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Positive 
  • Salmonella  (507924)  Completed 
    •  Positive 
  • Salmonella  (983397)  Completed 
    •  Positive