National Toxicology Program

National Toxicology Program

Testing Status of 3,3'-Dimethylbenzidine dihydrochloride - 10894-N


CASRN: 612-82-8

Related: 3,3'-DIMETHYLBENZIDINE 119-93-7

Formula: C14-H16-N2.2Cl-H

Synonyms/Common Names

  • 1,1'-Biphenyl)-4,4'-diamine, 3,3'-dimethyl-, dihydrochloride

Known Uses

Dye; chemical intermediate for azo dyes; curing agent for urethane resins

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Dosed-Water)  (C99990)  Completed 
    • Rats: F344/N
  • 13-Week (Dosed-Water)  (C99990)  Completed 
    • Rats: F344/N

Long-Term Carcinogenicity

  • 2 years (Dosed-Water)  (C99990)  Report Complete 
    • TR-390 (NIH Number: 91-2845)  (Peer Review Approval 04/25/1990A )
      Toxicology and Carcinogenesis Studies of 3,3'-Dimethylbenzidine Dihydrochloride (CASRN 612-82-8) in F344/N Rats (Drinking Water Studies)
    • Rats: F344/N
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Clear Evidence 
    • Dose: RATS ONLY: 0,30,70,150 PPM (70,45,75,70 PER GROUP RESPECTIVELY).

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (979364)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Drosophila  (475156)  Completed 
    • Citation: Valencia, R., Mason, J.M., Woodruff, R.C., and Zimmering, S. Chemical mutagenesis testing in Drosophila: III. Results of 48 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 325-348
    • Sex-Linked Recessive Lethal Positive 
    • Reciprocal Translocation Negative 
  • Salmonella  (497788)  Completed 
    • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelmans, K. Salmonella mutagenicity tests. IV. Results from the testing of 300 chemicals Environ. Molec. Mutagen. Vol. 11 (Suppl 12) (1988) 1-158
    •  Positive 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.