National Toxicology Program

National Toxicology Program

Testing Status of p-Nitrotoluene - 10944-E


CASRN: 99-99-0

Formula: C7-H7-N-O2

Synonyms/Common Names

  • 1-Methyl-4-Nitrobenzene (9CI)
  • 4-Nitrotoluene

Known Uses

Synthesis of intermediates and explosives, stilbene and fuchsin dyes. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Gavage)  (C60537A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 2 weeks (Dosed-Feed)  (C60537B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Gavage)  (C60537A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0,25,360, M: 0,10,160 MG/KG/10 PER GROUP.
  • 13 weeks (Dosed-Feed)  (C60537B)  Report Complete 
    • TOX-23 (NIH Number: 93-3346)
      Toxicity Studies of o-,m-, and p- Nitrotoluenes (CASRNs 88-72-2, 99-08-1, 99-99-0) Administered in Dosed Feed to F344/N Rats and B6C3F1 Mice
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 625, 1250, 2500, 5000, 10000 PPM/10 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Dosed-Feed)  (C62537D)  Report Complete 
    • TR-498 (NIH Number: 02-4432)  (Peer Review Approval 05/03/2001A )
      Toxicology and Carcinogenesis Studies of p-Nitrotoluene (CASRN 99-99-0) in F344/N Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Equivocal Evidence 
      • Female Rats Some Evidence 
      • Male Mice Equivocal Evidence 
      • Female Mice No Evidence 
    • Dose: R&M: 0, 1250, 2500, OR 5000 PPM; 50/SEX/DOSE.

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage; Intravenous)  (S0157)  Completed 
    • Male Rats: F344/N
    • Dose: PO: 1.07,10.7,107 MG/KG; IV: 8 MG/KG.
  • Absorption Disposition Metabolism Elimination (Gavage)  (S0633)  Completed 
    • Citation: Comparative Metabolism and Excretion of 14[C] P- Nitrotoluene following Oral Administration to Fischer 344 Rats and B6C3F1 Mice.
    • Male and Female Rats: F344/N; Male and Female Mice: B6C3F1
    • Dose: all dose formulations were prepared at target volume of 5 mL/kg. Both were administered 2 and 200 mg/kg..

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (961037)  Completed 
    • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Mouse Lymphoma  (762326)  Completed 
    • Mice
    •  Positive 
  • Micronucleus  (A06877)  Completed 
    • Rats: Fischer 344
    • Male Negative 
  • Micronucleus  (A22897)  Completed 
    • Mice: B6C3F1
    • Male Negative 
  • Salmonella  (828809)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    •  Negative 

Organ Systems Toxicity

  • 14 days Immunotoxicity (Gavage)  (IMM87033)  Completed 
    • Female Mice: B6C3F1
    • Dose: 0, 200, 400, 600 MG/KG; POSITIVE CONTROL (TOLUENE) AT 600 MG/KG.
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.