National Toxicology Program

National Toxicology Program

Testing Status of tris(Aziridinyl)-phosphine sulfide (Thio-TEPA) - 11109-K


CASRN: 52-24-4

Formula: C6-H12-N3-P-S

Synonyms/Common Names

  • 1,1',1''-Phosphinothioylidynetrisaziridine (9CI)
  • thio-TEPA
  • Thiotepa
  • Triethylenethiophosphorotriamide

Known Uses

Treatment of megaloblastic anemia. Poultry feed supplement. In foods & beverages.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Intraperitoneal Injection)  (C01649)  Completed 
    • Rats: Sprague Dawley; Mice: B6C3F1
  • 13-Week (Intraperitoneal Injection)  (C01649)  Completed 
    • Rats: Sprague Dawley; Mice: B6C3F1
    • Dose: R: 0,.14,2.8, M: 0,.23,4.6 MG/KG/5 PER GROUP.

Long-Term Carcinogenicity

  • 2-Year (Intraperitoneal Injection)  (C01649)  Report Complete 
    • TR-058 (NIH Number: 78-1308)  (Peer Review Approval 04/26/1978A )
      Bioassay of Thio-TEPA for Possible Carcinogenicity (CASRN 52-24-4)
    • Rats: Sprague Dawley; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Positive 
      • Female Rats Positive 
      • Male Mice Positive 
      • Female Mice Positive 
    • Dose: R: 0,.7,1.4,2.8, M: 0,1.15,2.3 MG/KG/35 PER GROUP.

Genetic Toxicology

  • Salmonella  (373221)  Completed 
    • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelman, K. Salmonella Mutagenicity Tests V. Results from the Testing of 311 Chemicals Environ. Molec. Mutagen. Vol. 19 (Suppl 21) (1992) 2-141
    •  Positive 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.