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Testing Status of Benzethonium chloride 11146-F


CASRN: 121-54-0

Formula: C27-H42-N-O2.Cl

Synonyms/Common Names

  • N,N-Dimethyl-N-(2-(2-(4-(1,1,3,3-tetramethylbutyl)phenoxy)ethoxy)ethyl)-benzenemethanaminium chloride

Known Uses

Used primarily in cosmetics for its antimicrobial and cationic surfactant properties. Topical anti-infective (vet) and antiseptic. Germicide for cleansing food and dairy utensils. In controlling swimming pool algae and dandruff.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Topical Application)  (C61494A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Topical Application)  (C61494A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0, 1.56, 25.0 MG/KG/10 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Topical Application)  (C61494B)  Completed 
    • TR-438 (NIH Number: 95-3169)  (Peer Review Approval 06/21/1994A )
      Toxicology and Carcinogenesis Studies of Benzethonium Chloride (CASRN 121-54-0) in F344/N Rats and B6C3F1 Mice (Dermal Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R&M: 0, 0.15, 0.5, 1.5 MG/KG/50/GROUP.

Special Studies

  • Absorption Disposition Metabolism Elimination (Intravenous; Topical Application)  (S0573)  Completed 
    • Rats: F344/N

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (173525)  Completed 
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Negative 
  • Salmonella  (397650)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative 

Organ Systems Toxicity

  • Immunotoxicity (Topical Application)  (IMM89007)  Completed 
    • Citation: NTP Summary Reports on Immunotoxicology Vol. 1, No. 1-15: Assessment of Contact Hypersensitivity to Benzethonium Chloride in Female B6C3F1 Mice
    • Citation: Summary Reports on Immunotoxicology Vol. 1, No. 1-15
    • Female Mice: B6C3F1
    • Dose: 0, 1, 3, 10%.
  • 6 to 15 GD (COPULATORY PLUG = 0 GD) Short-Term in vivo Repro. Tox. (Gavage)  (T0091)  Completed 
    • Female Mice: Swiss