National Toxicology Program

National Toxicology Program

Testing Status of Luminol - 11551-P


CASRN: 521-31-3

Formula: C8-H7-N3-O2

Synonyms/Common Names

  • 5-Amino-2,3-dihydro-1,4-phthalazinedione (9CI)
  • 3-Aminophthalic hydrazide
  • O-Aminophthalhyddrazide

Known Uses

Detection of copper, iron, peroxides, and cyanides. Used as an investigative tool in the processing of crime scenes for the presence of trace blood patterns. Luminol-enhanced chemiluminescence probes have been used to quantify and characterize the secretion of oxygen by phagocytozing cells.

Chemical Properties

Toxicity Effects (HSDB)

Nomination Background

Special Studies

  • Metabolism (Gavage; Intravenous; Topical Application)  (S0797)  Completed 
    • Citation: Sanders JM, Chen LJ, Burka LT, Matthews HB. Metabolism and disposition of luminol in the rat. Xenobiotica. 2000 Mar;30(3):263-72.Pubmed Abstract
    • Male Rats: F344/N

Genetic Toxicology

  • Micronucleus  (A29092)  Completed 
    • Rats: Fischer 344
    • Male Negative 
  • Salmonella  (108538)  Completed 
    • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelman, K. Salmonella Mutagenicity Tests V. Results from the Testing of 311 Chemicals Environ. Molec. Mutagen. Vol. 19 (Suppl 21) (1992) 2-141
    •  Negative 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.