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Testing Status of Methotrexate C04671


CASRN: 59-05-2

Formula: C20-H22-N8-O5

Synonyms/Common Names

  • N-(4-(((2,4-Diamino-6-pteridinyl)methyl)methylamino)benzoyl)-L-glutamic acid (9CI)

Known Uses

Chemotherapeutic agent, antirheumatic (Merck 1989)

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Intraperitoneal Injection)  (C04671)  Completed 
    • Rats: Sprague Dawley; Mice: Swiss
  • 13-Week (Intraperitoneal Injection)  (C04671)  Completed 
    • Rats: Sprague Dawley; Mice: Swiss

Long-Term Carcinogenicity

  • 2-Year (Intraperitoneal Injection)  (C04671)  Completed 
    •   (Peer Review Approval NA) Citation: EK Weisburger, Bioassay Program for Carcinogenic Hazards of Cancer Chemotherapeutic Agents. Cancer 40:1935-1949 (1977)
    • Rats: Sprague Dawley; Mice: Swiss
    • Dose: R: 0, .15, .30, M: 0, .5, 1.0 MG/KG/25 PER GROUP.

Organ Systems Toxicity

  • Total Reproductive Capacity (Intraperitoneal Injection)  (TRC98039)  Completed 
    • Female Mice: SEC/E X C57B1/E
    • Dose: 100 MG/KG.