National Toxicology Program

National Toxicology Program

Testing Status of alpha-Pinene - M030014


CASRN: 80-56-8

Formula: C10-H16

Synonyms/Common Names

  • Acintene A
  • Cyclic Dexadiene
  • 2-Pinene
  • 2,6,6-Trimethylbicyclo(3.1.1)-2-hept-2-ene

Known Uses

Solvent for protective coatings, polishes, and waxes; synthesis of camphene, camphor, terpin hydrate, terpineol, synthetic pine oil, terpine esters and others; lube oil additives, flavoring odorant. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14 days (Inhalation)  (C20302)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 100, 200, 400, 800, or 1600 ppm; 5/sex/species/dose.
  • 13 weeks (Inhalation)  (C20302)  Completed 
    • TOX-81 (NIH Number: 16-5937)
      NTP Technical Report on the Toxicity Studies of alpha-Pinene (CASRN 80-56-8) Administered by Inhalation to F344/N Rats and B6C3F1/N Mice
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 25, 50, 100, 200, or 400 ppm; R: 20/sex/dose; M: 10/sex/dose.

Long-Term Carcinogenicity

  • 2 years (Inhalation)  (C20302B)  Histopath in progress 
    • Rats: Harlan Sprague-Dawley; Mice: B6C3F1
    • Dose: Rats: 0, 50, 100, or 200 ppm 50/sex/exposure concentration; Mice: 0, 100, 200, or 400 ppm 50/sex exposure concentration.

Special Studies

  • 7 days Toxicokinetic Study (Inhalation) Selected (K03014)  
    • 7-day inhalation
    • Male and Female Rats: Harlan Sprague-Dawley; Male and Female Mice: B6C3F1/N
    • Dose: 50 and 100 ppm.

Genetic Toxicology

  • Micronucleus  (A82162)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (A29697)  Completed 
    •  Negative 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.