National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-m200024

Testing Status of Formamide - M200024

 

CASRN: 75-12-7

Formula: C-H3-N-O

Synonyms/Common Names

  • Carbamaldehyde
  • Methanamide

Known Uses

Solvent, softener, intermediate in organic synthesis; water-soluble ink formulations. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Nomination Background

Short-Term Toxicity

  • 2 weeks (Gavage)  (C88123)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 160, 312, 625, 1250 OR 2500 MG/KG; 5/SEX/SPECIES/DOSE.
  • 13 weeks (Gavage)  (C88123)  Subchronic Toxicology Review 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 10, 20, 40, 80, OR 160 MG/KG; 10/SEX/SPECIES/DOSE.

Long-Term Carcinogenicity

  • 2 years (Gavage)  (C88123B)  Report Draft 
    • TR-541 (NIH Number: 08-5884)  (Peer Review Approval 05/16/2007A )
      Toxicology and Carcinogenesis Studies of Formamide (CASRN 75-12-7) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
    • Rats: F344/N; Mice: B6C3F1/N
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice Clear Evidence 
      • Female Mice Equivocal Evidence 
    • Dose: R&M: 0, 20, 40, OR 80 MG/KG; 50/SEX/DOSE.

Special Studies

  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0533)  Completed 
    • Male Rats: F344/N
    • Dose: 10 mg/kg.
  • Metabolism (in-vitro)  (S0540)  Completed 
    • Male and Female Rats: F344/N; Male and Female Mice: B6C3F1; Male Human (Cell Lines)
    • Dose: NA.
  • Toxicokinetic Study (Gavage; Intravenous)  (S0613)  Completed 
    • Rats; Mice; Rats; Mice
    • Dose: rats-IV: 10, 30, 90 mg/kg mice-IV: 20, 60, 100 mg/kg rats-gav: 36, 300, 600 mg/kg mice-gav:120, 1000, 2000 mg/kg.

Genetic Toxicology

  • Drosophila  (462104)  Completed 
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Drosophila  (763972)  Completed 
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Micronucleus  (A14799)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (096297)  Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Negative 
  • Salmonella  (799767)  Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Negative 
  • Salmonella  (A95988)  Completed 
    •  Negative 

Organ Systems Toxicity

  • Continuous Breeding (Legacy) (Dosed-Water)  (RACB90005)  Completed 
    • Citation: Final Report on the Reproductive Toxicity of Formamide in CD-1 Swiss Mice
    • Mice: Swiss
    • Dose: 0, 250, 500, 1000, 2000, 3000; 0, 100, 350, 750 PPM; 0, 100, 350, 750 PPM; 0, 100, 350, 750 PPM.
  • 6 to 19 GD Conventional Teratology (Gavage)  (TER97002)  Completed 
    • Citation: Final Report on the Developmental Toxicity of Formamide (CAS# 75-12-7) Administered by Gavage to Sprague-Dawley CD Rats on Gestational Days 6-19
    • Female Rats: Sprague Dawley
    • Dose: 0, 50, 100, OR 200 MG/KG/DAY.
  • 6 to 29 GD Conventional Teratology (Gavage)  (TER98002)  Completed 
    • Citation: Developmental Toxicity Evaluation of Formamide (CASRN 75-12-7) Administered by Gavage to New Zealand White Rabbits on Gestational Days 6 through 29.
    • Female Rabbit: New Zealand White
    • Dose: 0, 35, 70 OR 140 MG/KG/DAY.
  • 6 to 19 days GD Teratology Pilot Studies (Gavage)  (TRP97008)  Completed 
    • Female Rats: Sprague Dawley
    • Dose: 0, 62, 125, 250, 500, OR 1000 MG/KG/DAY.
  • 6 to 29 GD Teratology Pilot Studies (Gavage)  (TRP98002)  Completed 
    • Female Rabbit: New Zealand White
    • Dose: 0, 10, 20, 40, 80, OR 120 MG/KG/DAY.
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