National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-m20287

Testing Status of Pentabromodiphenyl Ether Mixture [DE-71 (Technical Grade)] - M20287

 

CASRN: 32534-81-9

Formula: C12-H5-Br5-O

Synonyms/Common Names

  • Benzene, 1,1'-oxybis-, pentabromo deriv. (9CI)
  • DE71
  • DE 71
  • Pentabromodiphenyl oxide
  • Pentabromophenoxybenzene

Known Uses

Flame retardant

Chemical Properties

Toxicity Effects (HSDB)

Nomination Background

Planned Carcinogenicity/Toxicity

  • GD 6 to PND 21 (gestation dams) (Gavage) Selected (C20287)  
    • BDE Toxicogenomics Study
    • Rats: Wistar Han
  • PND 1 to PND 4 (lactation dams) (Gavage) Selected (C20287)  
    • BDE Toxicogenomics Study
    • Rats: Wistar Han
  • PND 4 to PND 21 (female pups) (Gavage) Selected (C20287)  
    • BDE Toxicogenomics Study
    • Rats: Wistar Han
  • PND 4 to PND 21 (male pups) (Gavage) Selected (C20287)  
    • BDE Toxicogenomics Study
    • Rats: Wistar Han
  • 26 weeks (Dermal) Selected (C20209B)  
    • Mice: Tg/RASH2/CB6F1
  • 13 weeks (Gavage) Selected (C20287)  
    • BDE Toxicogenomics Study
    • Rats: Wistar Han; Mice: B6C3F1

Short-Term Toxicity

  • 13 weeks (Gavage)  (C20209)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 0.01, 5, 50, 100, or 500 mg/kg; R: 20/sex/dose; M: 10/sex/dose.
  • GD 6 to PND 21 (Gavage)  (C20287B)  Completed 
    • Tier One BDE TGMX Study
    • Rats: Wistar Han
    • Dose: 0, 0.1, 15, or 50 mg/kg; 10/group (Alion study number 2013-228-215).
  • GD6 to PND 21 (Gavage)  (C20287C)  Completed 
    • Rats: Wistar Han
    • Dose: 0, 0.1, 15, or 50 mg/kg; 10/group (Alion study number 2014-060-328).

Long-Term Carcinogenicity

  • 2 years (Gavage)  (C20209)  Report Complete 
    • TR-589 (NIH Number: 16-5931)  (Peer Review Approval 06/25/2015A )
      Toxicology Studies of a Pentabromodiphenyl Ether Mixture [DE-71 (Technical Grade)] (CASRN 32534-81-9) in F344/N Rats and B6C3F1/N Mice and Toxicology and Carcinogenesis Studies of a Pentabromodiphenyl Ether Mixture [DE-71 (Technical Grade)] in Wistar Han [Crl:WI(Han)] Rats and B6C3F1/N Mice (Gavage Studies)
    • Rats: Wistar Han; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Clear Evidence 
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: M: 0, 3, 30, or 100 mg/kg; 50/sex/dose. R: 0, 3, 15, or 50 mg/kg; 50/sex/dose.

Special Studies

  • C. elegans  (W20287)  Completed 
    • C. elegans: N2

Genetic Toxicology

  • Micronucleus  (A27878)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Micronucleus  (A34110)  Completed 
    • Citation: Witt KL, Livanos E, Kissling GE, Torous DK, Caspary W, Tice RR, Recio L. Comparison of flow cytometry- and microscopy-based methods for measuring micronucleated reticulocyte frequencies in rodents treated with nongenotoxic and genotoxic chemicals. Mutat Res. 2008 Jan 8;649(1-2):101-13. Epub 2007 Aug 11.
    • Mice: B6C3F1
    • Male Negative 
  • Micronucleus  (F34110)  Completed 
    • Citation: Witt KL, Livanos E, Kissling GE, Torous DK, Caspary W, Tice RR, Recio L. Comparison of flow cytometry- and microscopy-based methods for measuring micronucleated reticulocyte frequencies in rodents treated with nongenotoxic and genotoxic chemicals. Mutat Res. 2008 Jan 8;649(1-2):101-13. Epub 2007 Aug 11.
    • Mice: B6C3F1
    • Male Negative 
    • Female NA 
  • Salmonella  (873255)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative 
  • Salmonella  (A82572)  Completed 
    •  Negative 
  • Salmonella  (A83261)  Completed 
    •  Negative 

Toxicogenomics

  • Microarray Analysis (Gavage) Selected (T20287B)  
    • Tier One BDE TGMX Study
    • Rats: Wistar Han
  • 5 days Microarray Analysis (Gavage) Selected (T20287C)  
    • 5-Day Transcriptomic Dose-Response Studies
    • Rats: Harlan Sprague-Dawley
    • Dose: 0, 0.4, 0.75, 1.5, 3, 15, 50, 100, or 200 mg/kg.
  • 6 GD - PND 21, 2 years Microarray Analysis (Gavage)  (T20287)  Completed 
    • Rats: Wistar Han
    • Dose: Control and high dose; 5 male pups, 5 female pups, and 5 male rats/dose group.
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.