National Toxicology Program

National Toxicology Program

Testing Status of Aspartame - M88167


CASRN: 22839-47-0


Formula: C14-H18-N2-O5

Synonyms/Common Names

  • L-Phenylalanine, N-L-alpha-aspartyl-, 1-methyl ester

Known Uses

Artificial sweetener

Chemical Properties

Toxicity Effects (HSDB)

Genetic Toxicology

  • Micronucleus  (A20159)  Completed 
    • Mice: P53 +/- (C57BL/6)
    • Male Negative 
    • Female Positive 
  • Micronucleus  (A37530)  Completed 
    • Rats: Fischer 344
    • Male Negative 
  • Micronucleus  (A56067)  Completed 
    • Mice: Tg.AC (FVB/N) Hemizygous
    • Male Negative 
    • Female Negative 
  • Micronucleus  (A66023)  Completed 
    • Mice: P16(Ink4a)/(+/-) (C57BL/6)
    • Male Negative 
    • Female Negative 
  • Micronucleus  (F37530)  Completed 
    • Rats: Fischer 344
    • Male Negative 
    • Female NA 
  • Salmonella  (A13862)  Completed 
    •  Negative 

Organ Systems Toxicity

  • Neurotoxicology Assessment (Gavage)  (NTA94035)  Completed 
    • Citation: Animal Neurobehavioral Test Battery in NTP Assessment In: Advances in Neurobehavioral Toxicology: Applications in Environmental & Occupational Health (Barry L. Johnson, ED.), Lewis Publishers, Inc. pp. 403-418 (1990).
    • Male Rats: F344/N
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.