National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-m882482

Testing Status of 3,3',4,4'-Tetrachloroazoxybenzene - M882482

 

CASRN: 21232-47-3

Related: 3,3',4,4'-TETRACHLOROAZOBENZENE

Formula: C12-H6-Cl4-N2-O

Synonyms/Common Names

  • Diazene, bis(3,4-dichlorophenyl)-, 1-oxide (9CI)
  • TCAOB

Known Uses

Impurity in dichloroaniline and herbicides derived from DCA

Chemical Properties

Toxicity Effects (HSDB)

Nomination Background

Short-Term Toxicity

  • 2 weeks (Gavage)  (C88149)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 12.5, 32, 80, 200 OR 500 MG/KG BODY WEIGHT DAY; M: 1, 3.2, 10, 32 OR 100 MG/KG BODY WEIGHT DAY PLUS CONTROLS (M&F; 5/GROUP).
  • 13 weeks (Gavage)  (C88149)  Report Complete 
    • TOX-66 (NIH Number: 99-3946)  (Tox Peer Review 06/18/1998A )
      Toxicity Studies of 3,3',4,4'-Tetrachloroazoxybenzene Administered by Gavage to F344/N Rats and B6C3F1 Mice (CASRN 21232-47-3)
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 0.1, 1.0, 3.0, 10, OR 30 MG/KG BODY WEIGHT (M&F 10/GROUP).

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage)  (S0296)  Completed 
    • Male Rats: F344/N
    • Dose: 320, 32, 3.2 MG/KG.
  • Metabolism (Gavage; Intravenous)  (S0808)  Completed 
    • Citation: Ziegler TL, Pillai UA, Smith RL, Kattnig MJ, Liebler DC, Mayersohn M, Sipes IG. Absorption and disposition kinetics of 3,3',4,4'-tetrachloroazoxybenzene in the male Fischer 344 rat. Drug Metab Dispos. 1996 Sep;24(9):1009-14.Pubmed Abstract
    • Male Rats: F344/N
    • Dose: [14C]TCAOB (3.4 and 34 mg/kg, 50 uCi/kg) in corn oil was administered (5 ml/kg). [14C]TCAOB (3.4 mg/kg) was administered intravenously (2 ml/kg). TCAOB was adminstered intravenously into the jugular vein(3.4 mg/kg, 2 ml/kg) or orally (34 mg/kg, 5 ml/kg); dosing vehicle was the solution of emulphor:ethanol:water [1:1:1 (v/v/v)]..
  • Metabolism (Gavage; Intravenous)  (S0809)  Completed 
    • Citation: Pillai UA, Ziegler TL, Wang DX, Kattnig MJ, McClure T, Liebler DC, Mayersohn M, Sipes IG. 3,3',4,4'-tetrachloroazobenzene absorption, disposition, and metabolism in male Fischer 344 rats. Drug Metab Dispos. 1996 Feb;24(2):238-44.Pubmed Abstract
    • Male Rats: F344/N
    • Dose: [14C]TCAB (3.2 or 32 mg/kg; 5 ml/kg) in corn oil was administered by gavage. All received 40-50 uCi/kg. [14C]TCAB (3.2 mg/kg; 2 ml/kg) was dissolved in a mixture of emulphor:ehthanol:water (3:3:4) by heating in an 80 degree C water bath. The solution was administered as abolus dose in the jugular vein. Unlabeled TCAB (3.2 mg/kg; 2 ml/kg) was administered intravenously to JVC rat..

Genetic Toxicology

  • Micronucleus  (A27535)  Completed 
    • Citation: Witt, K.L., Zeiger, E., Tice, R.R., and van Birgelen, A.P.J.M. The genetic toxicity of 3,3',4,4'-tetrachloroazobenzene and 3,3',4,4'-tetrachloroazoxybenzene: discordance between acute mouse bone marrow and subchronic mouse peripheral blood micronucleus test results. Mutation Res. Vol. 472 (2000) 147-154
    • Mice: B6C3F1
    • Male Positive 
    • Female Positive 
  • Micronucleus  (A78292)  Completed 
    • Citation: Witt, K.L., Zeiger, E., Tice, R.R., and van Birgelen, A.P.J.M. The genetic toxicity of 3,3',4,4'-tetrachloroazobenzene and 3,3',4,4'-tetrachloroazoxybenzene: discordance between acute mouse bone marrow and subchronic mouse peripheral blood micronucleus test results. Mutation Res. Vol. 472 (2000) 147-154
    • Mice: B6C3F1
    • Male Negative 
  • Salmonella  (A88322)  Completed 
    •  Negative 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.