National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-m930063

Testing Status of Diisopropylcarbodiimide - M930063

 

CASRN: 693-13-0

Formula: C7-H14-N2

Synonyms/Common Names

  • DIC
  • 1,3-Diisopropylcarbodiimide
  • N,N'-Diisopropylcarbodiimide

Known Uses

As a peptide coupling reagent; as a condensing reagent in dipeptide synthesis; in preparation of polyimide precursor coatings for electrophoretic image display fabrication; as a military nerve agent stabilizer.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 2 weeks (Topical Application)  (C93020)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 10, 30, 90, 269, OR 806 MG/ML; 5/SEX/SPECIES/GROUP.
  • 20 weeks (Topical Application)  (C93020B)  Report Complete 
    • GMM-10 (NIH Number: 07-4427)  (Peer Review Approval 09/28/2005A )
      Toxicology Study of Diispropylcarbodiimide (CASRN 693-13-0) in Genetically Modified (FVB Tg.AC Hemizygous) Mice and Carcinogenicity Study of Diispropylcarbodiimide in Genetically Modified [B6.129-Trp53tm1Brd (N5) Haploinsufficient Mice (Dermal Studies)
    • Mice: Tg.AC (FVB/N) Hemizygous
    • Dose: FEMALE MICE: 0, 4.38, 8.75, 17.5, 35, OR 70 MG/KG; 10/GROUP.
  • 27 weeks (Topical Application)  (C93020C)  Report Complete 
    • GMM-10 (NIH Number: 07-4427)  (Peer Review Approval 09/28/2005A )
      Toxicology Study of Diispropylcarbodiimide (CASRN 693-13-0) in Genetically Modified (FVB Tg.AC Hemizygous) Mice and Carcinogenicity Study of Diispropylcarbodiimide in Genetically Modified [B6.129-Trp53tm1Brd (N5) Haploinsufficient Mice (Dermal Studies)
    • Mice: P53 +/- (C57BL/6)
    • Dose: FEMALE MICE: 0, 4.38, 8.75, 17.5, 35, OR 70 MG/KG; 15/GROUP.
  • 13 weeks (Topical Application)  (C93020)  Report Complete 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 10, 20, 40, 80, OR 160 MG/KG M: 0, 17.5, 35, 70, 140, OR 280 10 SEX/SPECIES/GROUP, 6 GROUPS.

Long-Term Carcinogenicity

  • 2 years (Topical Application)  (C93020D)  Report Complete 
    • TR-523 (NIH Number: 07-4473)  (Peer Review Approval 09/28/2005A )
      Toxicology and Carcinogenesis Studies of Diisopropylcarbodiimide (CASRN 693-13-0) in F344/N Rats and B6C3F1 Mice (Dermal Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R&M: 0, 10, 20, OR 40 MG/KG; 50/SEX/SPECIES.

Special Studies

  • Absorption Disposition Metabolism Elimination (Intravenous; Topical Application)  (S0662)  Completed 
    • Citation: Disposition of Dermally and Intravenously Administered [14C]Dicyclohexylcarbodlimide in Fischer 344 Rats and B6C3F1 Mice January 4, 1999
    • Male Rats: F344/N; Male Mice: B6C3F1
    • Dose: dermal: 4-42 mg/kg (rats); 7-82 mg/kg (mice) IV: 8 mg/kg (dose vol. 1.3 ml/kg) (mice-only) for dermal needed to use charcoal-impregnated cellulose filters.

Genetic Toxicology

  • Micronucleus  (A04322)  Completed 
    • Citation: Witt, K.L., Tice, R.R., Shelby, M.D., Chhabra, R.S., and Zeiger, E. Induction of Micronucleated Erythrocytes in Rodents by Diisopropylcarbodiimide and Dicyclohexylcarbodiimide: Dependence on Exposure Protocol. Environ. Molec. Mutag. Vol. 33 (1999) 65-74
    • Mice: B6C3F1
    • Male Equivocal 
  • Micronucleus  (A22799)  Completed 
    • Citation: Witt, K.L., Tice, R.R., Shelby, M.D., Chhabra, R.S., and Zeiger, E. Induction of Micronucleated Erythrocytes in Rodents by Diisopropylcarbodiimide and Dicyclohexylcarbodiimide: Dependence on Exposure Protocol. Environ. Molec. Mutag. Vol. 33 (1999) 65-74
    • Mice: B6C3F1
    • Male Positive 
    • Female Positive 
  • Micronucleus  (A55884)  Completed 
    • Citation: Witt, K.L., Tice, R.R., Shelby, M.D., Chhabra, R.S., and Zeiger, E. Induction of Micronucleated Erythrocytes in Rodents by Diisopropylcarbodiimide and Dicyclohexylcarbodiimide: Dependence on Exposure Protocol. Environ. Molec. Mutag. Vol. 33 (1999) 65-74
    • Mice: B6C3F1
    • Male Positive 
  • Micronucleus  (A76999)  Completed 
    • Citation: Witt, K.L., Tice, R.R., Shelby, M.D., Chhabra, R.S., and Zeiger, E. Induction of Micronucleated Erythrocytes in Rodents by Diisopropylcarbodiimide and Dicyclohexylcarbodiimide: Dependence on Exposure Protocol. Environ. Molec. Mutag. Vol. 33 (1999) 65-74
    • Rats: Fischer 344
    • Male Negative 
  • Salmonella  (A69920)  Completed 
    •  Equivocal 
  • Salmonella  (A92772)  Completed 
    •  Negative 

Organ Systems Toxicity

  • 4 days Immunotoxicity (Topical Application)  (IMM95010)  Completed 
    • Female Mice: B6C3F1
    • Dose: .03, .15, .3, 1.5, AND 3% DIC IN ACETONE (99.8%) FOR SENSIZATION, AND 10% DIC FOR CHALLENGE IN THE MEST AND 1.5, 3 AND 10% DIC IN THE LLNA. .25% DNFB USED AS POSITIVE CONTROL FOR SENSITIZATION, AND .5% FOR CHALLENGE..
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.