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Testing Status of 1,2-Dibromo-2,4-dicyanobutane M950118

 

CASRN: 35691-65-7

Formula: C6-H6-Br2-N2

Synonyms/Common Names

  • 2-Bromo-2-(bromomethyl)glutaronitrile
  • 2-Bromo-2-(bromomethyl)pentanedinitrile
  • Dibromodicyanobutane
  • Metacide 38
  • Methyldibromoglutaronitrile
  • Tektamer 38

Short-Term Toxicity

  • 2 weeks (Topical Application)  (C97003B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 37, 75, 150, 300, OR 600 MG/KG (GIVEN IN TWO DOSES); M: 0, 75, 150, 300, 600, OR 1200 MG/KG; 5/SEX/SPECIES/DOSE.
  • 2 weeks (Gavage)  (C97003)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 37, 75, 150, 300, OR 600 MG/KG; M: 0, 75, 150, 300, 600, OR 1200 MG/KG; 5/SEX/SPECIES/DOSE.
  • 13 weeks (Topical Application)  (C97003C)  Completed 
    • Rats: F344/N; Mice: B6C3F1/N
    • Dose: R&M: 0, 0.2, 0.6, 2, 6, OR 18 MG/KG, 5X PER WEEK; 10/SEX/DOSE.

Long-Term Carcinogenicity

  • 2 years (Topical Application)  (C97003D)  Completed 
    • TR-555 (NIH Number: 10-5896)  (Peer Review Approval 02/27/2008A )
      Toxicology and Carcinogenesis Studies of 1,2-Dibromo-2,4-dicyanobutane (CASRN 35691-65-7) in F344/N Rats and B6C3F1 Mice (Dermal Studies)
    • Rats: F344/N; Mice: B6C3F1/N
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R: 0, 2, 6, OR 18 MG/KG AND M: 0, 0.6, 2, OR 6 MG/KG; 50/SEX/SPECIES/D0SE.

Special Studies

  • Mechanisms (Intravenous)  (S0783)  Completed 
    • Citation: Sauer JM, Smith RL, Bao J, Kattnig MJ, Kuester RK, Sipes IG. Metabolic and dispositional fate of 1,2-dibromo-2,4-dicyanobutane in the male fischer 344 Rat. Drug Metab Dispos. 1998 May;26(5):429-36.Pubmed Abstract
    • Male Rats: F344/N
  • Mechanisms (Intraperitoneal Injection)  (S0784)  Completed 
    • Citation: Bao J, Sauer JM, Smith RL, Kuester RK, Kattnig MJ, Sipes IG. Sulfhydryl-dependent biotransformation and macromolecular binding of 1,2-dibromo-2,4-dicyanobutane in blood. Drug Metab Dispos. 1998 Oct;26(10):1001-7.Pubmed Abstract
    • Male Rats: F344/N
  • Metabolism (Intravenous)  (S0806)  Completed 
    • Citation: Bao J, Sauer JM, Smith RL, Kuester RK, Kattnig MJ, Sipes IG. Sulfhydryl-dependent biotransformation and macromolecular binding of 1,2-dibromo-2,4-dicyanobutane in blood. Drug Metab Dispos. 1998 Oct;26(10):1001-7.Pubmed Abstract
    • Male Rats: F344/N
    • Dose: [14C]BCB (80 mg/kg, 120 uCi/kg, 2 ml/kg), in a vehicle of 10% emulphor/15% ethanol/75% saline solution was administered via the indwell-ing jugular vein cannula..
  • Metabolism (Gavage; Intravenous; Topical Application)  (S0807)  Completed 
    • Citation: Sauer JM, Smith RL, Bao J, Kattnig MJ, Kuester RK, Sipes IG. Metabolic and dispositional fate of 1,2-dibromo-2,4-dicyanobutane in the male fischer 344 Rat. Drug Metab Dispos. 1998 May;26(5):429-36.Pubmed Abstract
    • Male Rats: F344/N
    • Dose: [14C]BCB (8 mg/kg, 120 uCi/kg), in emulphor/ethanol/saline, 3:4:12 (v/v/v) was administered via a jugular vein cannula; [14C]BCB (80 mg/kg, 100 uCi/kg) in corn oil (5 ml/kg) was administered orally by gavage; 100 ul of [14C]BCB (25 mg/kg, 50 uCi/kg) in ethanol/propylene glycol, 3:7 (v/v) was applied to the shaved backs of the animals..

Genetic Toxicology

  • Micronucleus  (A65692)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (A11889)  Completed 
    •  Negative 
  • Salmonella  (A92777)  Completed 
    •  Negative