National Toxicology Program

National Toxicology Program

Testing Status of Acetoin - M990018


CASRN: 513-86-0

Related: Artificial Butter Flavoring Mixture

Formula: C4-H8-O2

Synonyms/Common Names

  • Acetyl methyl carbinol
  • 3-Hydroxy-2-butanone

Known Uses

Found in cheddar cheese; used in flavoring & fragrances. Aroma carrier; preparation of flavors & essences. Used for flavoring margarine. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14 days (Inhalation)  (C20724)  Completed 
    • Rats: Wistar Han; Mice: B6C3F1
    • Dose: 0, 6.25, 25, 100, 400, or 800 ppm; 5/sex/species/conc.
  • 90 days (Inhalation)  (C20724)  Completed 
    • Rats: Wistar Han; Mice: B6C3F1
    • Dose: R&M: 0, 50, 100, 200, 400, or 800 ppm -- R: 20/sex/conc; M: 10/sex/conc.

Genetic Toxicology

  • Micronucleus  (G99018B)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Micronucleus  (G99018)  Completed 
    • Rats: Wistar Han
    • Male Negative 
    • Female Negative 
  • Salmonella  (A17696)  Completed 
    •  Negative 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.