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Abstract for IMM88035

Assessment of Contact Hypersensitivity to Crotonaldehyde in Female B6C3F1 Mice

CASRN: 4170-30-3
Chemical Formula: C4H6O
Molecular Weight: 70.0904
Report Date: November 1989


The following abstract presents results of a study conducted by a contract laboratory for the National Toxicology Program. The findings have not been peer reviewed and were not evaluated in accordance with the levels of evidence criteria established by NTP in March 2009. The findings and conclusions for this study should not be construed to represent the views of the NTP or the U.S. Government.

Crotonaldehyde was selected for evaluation as a sensitizing agent for contact hypersensitivity in guinea pigs and mice. CRA is used as an intermediate in the manufacture of n-butanol, crotonic acid and sorbic acids; in resin and rubber antioxidant manufacture; also as a solvent in mineral oil purification; as a warning agent in fuel gas; as an alcohol denaturant; in the manufacture of butyraldehyde, quinaldine; in locating breaks and leaks in pipes; minor amounts are Used in manufacture of maleic acid, crotyl alcohol, butyl chloral hydrate and in rubber accelerators; in organic synthesis, in insecticides; and in chemical warfare.

The objective of this study was to determine the sensitizing potential of crotonaldehyde when applied dermally to female B6C3F1 mice.

Crotonaldehyde was tested on female B6C3F1 mice. The doses of crotonaldehyde ranged from 0.3% to 3.0% in a solution of 4 parts acetone to one part olive oil (4:1) for sensitization and 10% for challenge. Mice received 20 μl by direct dermal application for 5 consecutive days to a prepared site. 2,4-dinitrofluorobenzene, (99.6%) was used as a positive control at a concentration of 0.5%. Measurement of the contact hypersensitivity was accomplished by the radioisotopic assay.

A dose-dependent contact hypersensitivity response to crotonaldehyde could not be demonstrated in mice.