National Toxicology Program

National Toxicology Program

Abstract from Report IMM91028 on 2-Methoxyethanol

Report on the Assessment of Contact Hypersensitivity of 2-Methoxyethanol in Female B6C3F1 Mice (CAS No. 109-86-4)

Report Date: July 1991

The following abstract presents results of a study conducted by a contract laboratory for the National Toxicology Program. The findings have not been peer reviewed and were not evaluated in accordance with the levels of evidence criteria established by NTP in March 2009. The findings and conclusions for this study should not be construed to represent the views of the NTP or the U.S. Government.


2-Methoxyethanol, also known as methyl cellosolve or ethylene glycol monomethyl ether, was selected for evaluation as a sensitizing agent for contact hypersensitivity in mice. MEL is a solvent for low-viscosity cullulose acetate, natural resins, someI synthetic resins and some alcohol-soluble dyes. It is also used in dyeing leather, sealing moistureproof cellophane; in nail polishes, quick-drying varnishes and enamels, and wood stains.

The objective of this study was to determine the sensitizing potential of 2-methoxyethanol when applied dermally to female B6C3F1 mice.

2-Methoxyethanol was tested on female B6C3F1 mice. The doses of 2-methoxyethanol ranged from 3.0% to 30% in acetone/olive oil for sensitization and 30% for challenge. Mice received 20 ┬Ál by direct dermal application for 5 consecutive days to a prepared site. Site preparation included intradermal injection of Freund's complete adjuvant in some mice. DNFB (1-fluoro-2,4-dinitrobenzene) was used as a positive control at a concentration of 0.25% for sensitization and 0.5% for challenge. Measurement of the contact hypersensitivity response was accomplished by the radioisotopic assay and the Mouse Ear Swelling Test.

No statistically significant (p<0.05) or dose-dependent (p<0.05) contact hypersensitivity response-to 2-methoxyethanol was demonstrated by either the radioisotopic assay or MEST.

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