Skip to Main Navigation
Skip to Page Content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Testing Status of 4-Vinylcyclohexene 10480-T


CASRN: 100-40-3

Formula: C8-H12

Synonyms/Common Names

  • 4-Ethenylcyclohexene (9CI)

Short-Term Toxicity

  • 14-Day (Gavage)  (C54999)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Gavage)  (C54999)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 50, 800, M: 0, 75, 1200 MG/KG/10 PER GROUP.

Long-Term Carcinogenicity

  • 2-Year (Gavage)  (C54999)  Completed 
    • TR-303 (NIH Number: 86-2559)  (Peer Review Approval 03/29/1985A )
      Toxicology and Carcinogenesis Studies of 4-Vinylcyclohexene (CASRN 100-40-3) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Inadequate Study 
      • Female Rats Inadequate Study 
      • Male Mice Inadequate Study 
      • Female Mice Clear Evidence 
    • Dose: 0, 200, 400 MG/KG/50 PER GROUP.

Special Studies

  • Absorption Distribution Metabolism Excretion (Gavage)  (S0084)  Completed 
    • Citation: Smith BJ, Carter DE, Sipes IG. Comparison of the disposition and in vitro metabolism of 4-vinylcyclohexene in the female mouse and rat. Toxicol Appl Pharmacol. 1990 Sep 15;105(3):364-71.Pubmed Abstract
    • Citation: Smith BJ, Mattison DR, Sipes IG. The role of epoxidation in 4-vinylcyclohexene-induced ovarian toxicity. Toxicol Appl Pharmacol. 1990 Sep 15;105(3):372-81. Pubmed Abstract
    • Female Rats: Fischer 344; Female Mice: B6C3F1
    • Dose: 5 mg/ml. 50 mg/ml.
  • Metabolism (Intraperitoneal Injection)  (S0800)  Completed 
    • Citation: Doerr JK, Hooser SB, Smith BJ, Sipes IG. Ovarian toxicity of 4-vinylcyclohexene and related olefins in B6C3F1 mice: role of diepoxides. Chem Res Toxicol. 1995 Oct-Nov;8(7):963-9.Pubmed Abstract
    • Female Mice: B6C3F1
    • Dose: Structure-Activity Studies: at either a dose equimolar to that of VCH (7.5 mmol/kg), or at a lower dose which had been determined fromprevious studies as the maximum tolerated dose by mice over 30 day period w/o significant weight loss. In Vivo Metabolism Studies: The mice were administered a single dose (7.5 mmol/kg), ip, of test com-pound in sesame seed oil..
  • Metabolism (Intraperitoneal Injection)  (S0801)  Completed 
    • Citation: Doerr-Stevens JK, Liu J, Stevens GJ, Kraner JC, Fontaine SM, Halpert JR, Sipes IG. Induction of cytochrome P-450 enzymes after repeated exposure to 4-vinylcyclohexene in B6C3F1 mice. Drug Metab Dispos. 1999 Feb;27(2):281-7.Pubmed Abstract
    • Female Mice: B6C3F1
    • Dose: VCH (7.5 mmol/ky/day x 10 days i.p.), sesame seed oil (vehicle control, 2.5 ml/kg/day x 10 days i.p.), PB (80 mg/kg/day x 5 days i.p.), DEX (100 mg/kg/day x 3 days i.p.), or acetone (ACE) [in drinking water 1 % (v/v) x 5 days]. PB was made up as 3.2% solution (w/v) in 0.9% NaCl, and DEX was in a 2% Tween 80 solution (v/v)..

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (169960)  Completed 
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Negative 
  • Mouse Lymphoma  (971117)  Completed 
    • Mice
    •  Positive 
  • Salmonella  (609542)  Completed 
    •  Negative 
  • Salmonella  (777152)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative 

Organ Systems Toxicity

  • 27 weeks Continuous Breeding (Legacy) (Gavage)  (RACB90035)  Completed 
    • Male and Female Mice: Swiss
    • Dose: N/A; 0, 100, 250, 500 MG/KG/DAY; N/A; 0, 500 MG/KG/DAY; .
  • single Total Reproductive Capacity (Intraperitoneal Injection)  (TRC98044)  Completed 
    • Female Mice: (SEC/E X C57BL/E)F1
    • Dose: 2000 MG/KG.