Testing Status of 1,2-Dibromoethane 10513-F

CASRN: 106-93-4

Formula: C2-H4-Br2

Synonyms/Common Names

• EDB
• Ethylene dibromide

Short-Term Toxicity

• 14-Day (Inhalation)  (C00522B)  Completed 
  • Rats: F344/N; Mice: B6C3F1
• 13 weeks (Gavage)  (C00522A)  Completed 
  • Rats: Osborne Mendel; Mice: B6C3F1
  • Dose: 0, 40, 251 MG/KG/5 PER GROUP.
• 13-Week (Inhalation)  (C00522B)  Completed 
  • Rats: F344/N; Mice: B6C3F1
  • Dose: 0, 3, 75 PPM/10R - 20M PER GROUP.

Long-Term Carcinogenicity

• 2 years (Gavage)  (C00522A)  Completed 
  • TR-086 (NIH Number: 78-1336)  (Peer Review Approval 04/26/1978A )
    Bioassay of 1,2-Dibromoethane for Possible Carcinogenicity (CASRN 106-93-4)
  • Rats: Osborne Mendel; Mice: B6C3F1
  • Carcinogenesis Results
    • Male Rats Positive 
    • Female Rats Positive 
    • Male Mice Positive 
    • Female Mice Positive 
  • Dose: MR: 0, 38, 41 (AVG), FR: 0, 37, 39 (AVG), M: 0, 62, 107 MG/KG/50 PER GROUP.
• 2-Year (Inhalation)  (C00522B)  Completed 
  • TR-210 (NIH Number: 82-1766)  (Peer Review Approval 06/27/1980A )
    Carcinogenesis Bioassay of 1,2-Dibromoethane (CASRN 106-93-4) in F344 Rats and B6C3F1 Mice (Inhalation Study)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
    • Male Rats Positive 
    • Female Rats Positive 
    • Male Mice Positive 
    • Female Mice Positive 
  • Dose: 0, 10, 40 PPM/50 PER GROUP.

Special Studies

• Cell Proliferation (Gavage)  (S0762)  Completed 
  • Citation: Ghanayem BI, Maronpot RR, Matthews HB. Association of chemically induced forestomach cell proliferation and carcinogenesis. Cancer Lett. 1986 Sep;32(3):271-8.Pubmed Abstract
  • Male Rats: F344/N

Genetic Toxicology

• Chromosome Aberrations  (738945)  Completed 
  • Mice
  •  Negative 
• Drosophila  (782145)  Completed 
  • Citation: Foureman, P., Mason, J.M., Valencia, R., Zimmering, S. Chemical Mutagenesis Testing in Drosophila. X. Results of 70 coded compounds tested for the National Toxicology Program. Environ. Molec. Mutagen. Vol. 23 (1994) 208-227
  • Sex-Linked Recessive Lethal Positive 
  • Reciprocal Translocation Positive 
• In Vitro Cytogenetics (CA/SCE)  (665769)  Completed 
  • Citation: Ivett, J.L., Brown, B.M., Rodgers, C., Anderson, B.E., Resnick, M.A., Zeiger, E. Chromosomal aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro IV: Results for 15 chemicals Environ. Molec. Mutagen. Vol. 14 (1989) 165-187
  • Sister Chromatid Exchange Positive 
  • Chromosome Aberrations Positive 
• Micronucleus  (A71010)  Completed 
  • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
  • Mice: B6C3F1
  • Male Positive 
• Mouse Lymphoma  (G50253)  Completed 
  • Citation: Mitchell, A.D., Rudd, C.J., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for 63 coded chemicals tested at SRI International. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 37-102
  • Mice
• Mouse Lymphoma  (G62050)  Completed 
  • Citation: Myhr, B.C., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for sixty-three coded chemicals tested at Litton Bionetics, Inc. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 103-194
  • Mice
• Salmonella  (529327)  Completed 
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelman, K. Salmonella Mutagenicity Tests V. Results from the Testing of 311 Chemicals Environ. Molec. Mutagen. Vol. 19 (Suppl 21) (1992) 2-141
  •  Positive 
• Salmonella  (N-9282)  Completed 
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli Environ. Mutagen. Vol. 7 (Suppl 5) (1985) 1-248
• Sister Chromatid Exchanges  (738945)  Completed 
  • Mice
  • Sister Chromatid  Positive (Nonstandard Protocol) 

Organ Systems Toxicity

• Biochemical Specific Locus (Gavage)  (BSLM37)  Completed 
  • Citation: Barnett, L.B., Felton, C.F., Gibson, B.J., Sharpe, D.S., Shelby, M.D., and Lewis, S.E. No heritable mutations are induced by ethylene dibromide at electrophoretically expressed loci. Mutation Res. 282:127-133 (1992).
  • Male Mice: DBA/2J
  • Mice Male Negative 
• Dominant Lethal (Male) (Dosed-Feed)  (DLM021)  Completed 
  • Citation: Barnett, L.B., Felton, C.F., Gibson, B.J., Sharpe, D.S., Shelby, M.D., and Lewis, S.E. No heritable mutations are induced by ethylene dibromide at electrophoretically expressed loci. Mutation Res. 282:127-133 (1992).
  • Male Mice: DBA/2J
  • Mice Male Negative 
• Total Reproductive Capacity (Dosed-Feed)  (TRC98012)  Completed 
  • Citation: Bishop, JB, Morris, RW, Seely, JC, Hughes, LA, Cain, KT, Generoso, WM. Alterations in the Reproductive Patterns of Female Mice Exposed to Xenobiotics. Fund. and Appl. Tox. 40: 191-204 (1997).
  • Female Mice: B6C3F1
  • Mice Female Negative