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Testing Status of 2-Methoxy-4-nitroaniline M060049

 

CASRN: 97-52-9

Formula: C7-H8-N2-O3

Synonyms/Common Names

  • 2-Amino-5-nitroanisole
  • Azoamine Pink O
  • 4-Nitro-o-anisidine

Short-Term Toxicity

  • 14 days (Dosed-Feed)  (C06049)  Completed 
    • Citation: Frawley RP, Witt KL, Cunny H, Germolec DR, Jackson-Humbles D, Malarkey D, Shockley KR, Stout M, Travlos G, Buccellato M, Fallacara D, Harris S, Kissling GE, Manheng W, Surh II, White K Jr, Auerbach SS. Evaluation of 2-methoxy-4-nitroaniline (MNA) in hypersensitivity, 14-day subacute, reproductive, and genotoxicity studies. Toxicology. 2020 Aug;441:152474. doi: https://doi.org/10.1016/j.tox.2020.152474. Epub 2020 May 5.Pubmed Abstract
    • Mice: B6C3F1
    • Dose: R: 8/dose; M: 5/sex/dose; R&M: 0, 625, 1250, 2500, 5000, or 10000 ppm.
  • Perinatal range finding (Dosed-Feed)  (C06049)  Completed 
    • Citation: Frawley RP, Witt KL, Cunny H, Germolec DR, Jackson-Humbles D, Malarkey D, Shockley KR, Stout M, Travlos G, Buccellato M, Fallacara D, Harris S, Kissling GE, Manheng W, Surh II, White K Jr, Auerbach SS. Evaluation of 2-methoxy-4-nitroaniline (MNA) in hypersensitivity, 14-day subacute, reproductive, and genotoxicity studies. Toxicology. 2020 Aug;441:152474. doi: https://doi.org/10.1016/j.tox.2020.152474. Epub 2020 May 5.Pubmed Abstract
    • Rats: Harlan Sprague-Dawley
    • Dose: R: 8/dose; M: 5/sex/dose; R&M: 0, 625, 1250, 2500, 5000, or 10000 ppm.

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage; Topical Application; Intravenous; Gavage; Topical Application; Intravenous)  (K06049)  Completed 
    • Citation: Mathews JM, Zhan Q, Etheridge AS, Patel PR, Black SR, Banks TT, Fennell TR, Snyder RW, Burgess JP, Warren SD, Surh I, Waidyanatha S. Metabolism and disposition of 2-methoxy-4-nitroaniline in male and female Harlan Sprague Dawley rats and B6C3F1/N mice. Xenobiotica. 2012 Dec;42(12):1213-24. doi: 10.3109/00498254.2012.697211. Epub 2012 Jun 23.Pubmed Abstract
    • Male and Female Rats: Harlan Sprague-Dawley; Male Rats: Harlan Sprague-Dawley; Male and Female Rats: Harlan Sprague-Dawley; Male and Female Mice: B6C3F1; Male Mice: B6C3F1; Male and Female Mice: B6C3F1
    • Dose: gavage; 1.5, 15, 150 mg/kg. intravenous; 15 mg/kg. topical application; 15 mg/kg.

Genetic Toxicology

  • Comet Assay  (G06049C)  Completed 
    • Mice
  • Salmonella  (G06049)  Completed 
    •  Positive 

Organ Systems Toxicity

  • Varies Immunotoxicity (Dermal)  (I06049)  Completed 
    • Female Mice: BALB/c
    • Dose: 0, 2.5%, 5%, 10%, 25%, or 50%.