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Testing Status of Pentabromodiphenyl Ether Mixture [DE-71 (Technical Grade)] M20287

 

CASRN: 32534-81-9

Formula: C12-H5-Br5-O

Synonyms/Common Names

  • Benzene, 1,1'-oxybis-, pentabromo deriv. (9CI)
  • DE71
  • DE 71
  • Pentabromodiphenyl oxide
  • Pentabromophenoxybenzene

Short-Term Toxicity

  • 5 days (Gavage)  (C20287D)  Completed 
    • 5-Day Transcriptomic Dose-Response Studies
    • Citation: Gwinn WM, Auerbach SS, Parham F, Stout MD, Waidyanatha S, Mutlu E, Collins B, Paules RS, Merrick BA, Ferguson S, Ramaiahgari S, Bucher JR, Sparrow B, Toy H, Gorospe J, Machesky N, Shah RR, Balik-Meisner MR, Mav D, Phadke DP, Roberts G, DeVito MJ. Evaluation of 5-day In Vivo Rat Liver and Kidney With High-throughput Transcriptomics for Estimating Benchmark Doses of Apical Outcomes. Toxicol Sci. 2020 Aug 1;176(2):343-354. doi: https://doi.org/10.1093/toxsci/kfaa081.Pubmed Abstract
    • Rats: Harlan Sprague-Dawley
    • Dose: Male rats: 0, 0.076, 0.25, 0.3, 0.6, 3, 10, 20, 40, or 100 mg/kg; N=4.
  • 13 weeks (Gavage)  (C20209)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 0.01, 5, 50, 100, or 500 mg/kg; R: 20/sex/dose; M: 10/sex/dose.
  • GD 6 to PND 21 (Gavage)  (C20287B)  Completed 
    • Rats: Wistar Han
    • Dose: 0, 0.1, 15, or 50 mg/kg; 10/group.
  • GD 6 to PND 21 (Gavage)  (C20287C)  Completed 
    • In Utero - PND 22 Exposure to DE-71
    • Citation: Dunnick JK, Shockley KR, Morgan DL, Travlos GS, Gerrish K, Ton TT, Wilson R, Brar SS, Brix AE, Waidyanatha S, Mutlu E, Pandiri AKR. Hepatic Transcriptomic Patterns in the Neonatal Rat After Pentabromodiphenyl Ether Exposure. Toxicol Pathol. 2020 Feb;48(2):338-349. https://doi.org/10.1177/0192623319888433. Epub 2019 Dec 12.
    • Rats: Wistar Han
    • Dose: 0, 0.1, 15, or 50 mg/kg; 10/group.

Long-Term Carcinogenicity

  • 2 years (Gavage)  (C20209)  Completed 
    • TR-589 (NIH Number: 16-5931)  (Peer Review Approval 06/25/2015A )
      Toxicology Studies of a Pentabromodiphenyl Ether Mixture [DE-71 (Technical Grade)] (CASRN 32534-81-9) in F344/N Rats and B6C3F1/N Mice and Toxicology and Carcinogenesis Studies of a Pentabromodiphenyl Ether Mixture [DE-71 (Technical Grade)] in Wistar Han [Crl:WI(Han)] Rats and B6C3F1/N Mice (Gavage Studies)
    • Rats: Wistar Han; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Clear Evidence 
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: M: 0, 3, 30, or 100 mg/kg; 50/sex/dose. R: 0, 3, 15, or 50 mg/kg; 50/sex/dose.

Special Studies

  • C. elegans  (W20287)  Completed 
    • C. elegans: N2
    • Dose: Growth:0, 0.1, 0.16, 0.25, 0.40, 0.63, 1, 1.6, 2.5, 4, 6.3, 10uMRepro:0, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.3, 13, 25, 50, 100uMFeeding:0, 0.5, 0.79, 1.3, 2.0, 3.2, 5.0, 7.9, 13, 20, 32, 50uM.

Genetic Toxicology

  • Micronucleus  (A27878)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Micronucleus  (A34110)  Completed 
    • Citation: Witt KL, Livanos E, Kissling GE, Torous DK, Caspary W, Tice RR, Recio L. Comparison of flow cytometry- and microscopy-based methods for measuring micronucleated reticulocyte frequencies in rodents treated with nongenotoxic and genotoxic chemicals. Mutat Res. 2008 Jan 8;649(1-2):101-13. Epub 2007 Aug 11.
    • Mice: B6C3F1
    • Male Negative 
  • Micronucleus  (F34110)  Completed 
    • Citation: Witt KL, Livanos E, Kissling GE, Torous DK, Caspary W, Tice RR, Recio L. Comparison of flow cytometry- and microscopy-based methods for measuring micronucleated reticulocyte frequencies in rodents treated with nongenotoxic and genotoxic chemicals. Mutat Res. 2008 Jan 8;649(1-2):101-13. Epub 2007 Aug 11.
    • Mice: B6C3F1
    • Male Negative 
    • Female NA 
  • Salmonella  (873255)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative 
  • Salmonella  (A82572)  Completed 
    •  Negative 
  • Salmonella  (A83261)  Completed 
    •  Negative 

Toxicogenomics

  • 6 GD - PND 21, 2 years Microarray Analysis (Gavage)  (T20287)  Completed 
    • Rats: Wistar Han
    • Dose: Control and high dose; 5 male pups, 5 female pups, and 5 male rats/dose group.
  • Microarray Analysis (Gavage)  (T20287B)  Completed 
    • In Utero-PND 22 Exposure to DE-71
    • Rats: Wistar Han
  • 5 days Transcriptome Sequencing (Gavage)  (T20287C)  Completed 
    • 5-Day Transcriptomic Dose-Response Studies
    • Citation: Gwinn WM, Auerbach SS, Parham F, Stout MD, Waidyanatha S, Mutlu E, Collins B, Paules RS, Merrick BA, Ferguson S, Ramaiahgari S, Bucher JR, Sparrow B, Toy H, Gorospe J, Machesky N, Shah RR, Balik-Meisner MR, Mav D, Phadke DP, Roberts G, DeVito MJ. Evaluation of 5-day In Vivo Rat Liver and Kidney With High-throughput Transcriptomics for Estimating Benchmark Doses of Apical Outcomes. Toxicol Sci. 2020 Aug 1;176(2):343-354. doi: https://doi.org/10.1093/toxsci/kfaa081.Pubmed Abstract
    • Rats: Harlan Sprague-Dawley