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Tox21 Toolbox

The Tox21 Toolbox contains data-analysis tools for accessing and visualizing Tox21 quantitative high-throughput screening (qHTS) 10K library data, as well as integrating with other publicly available data.


  • Tox21 Data Analysis tools facilitate the analysis and visualization of Tox21 data.
    • BMDExpress 2 allows users to perform genomic dose-response analyses, with the goal of identifying gene set/pathway-level potency estimates.
  • Tox21 Data Visualization tools provide quick, easy-to-use Tox21 qHTS data access, visualization, and clustering.
    • Tox21 Curve Browser (beta version) lets users visualize the concentration-response curves of compounds of interest, with the ability to superimpose assay results per compound for easy comparison.
    • Tox21 Activity Profiler (beta version) lets users prioritize compounds through compound structure and/or Tox21 qHTS data activity clustering visualization. Plots and data are available for download.
  • Tox21 Data Integration applications integrate data from external resources to facilitate interpretation of Tox21 data.
    • Tox21 Enricher (beta version) allows users to upload a set of compounds and find enriched toxicological characteristics from biological data sources such as DrugMatrix®, PubChem, Comparative Toxicogenomics Database, and Leadscope.
    • Tox21 IVIVE Dose Equivalent (beta version) provides dose estimates for the likelihood of in vivo human biological interactions, using Tox21 data and high-throughput toxicokinetics modeling.
  • DrugMatrix® and ToxFX® tools increase the efficiency of the toxicological assessment process.
    • DrugMatrix® is one of the world's largest toxicogenomic reference resources.
    • ToxFX® is the companion database to DrugMatrix® and contains a reporting system that generates fully annotated reports in minutes.

External Tools

  • PubChem provides access to large-scale screening data (including, but not limited to, Tox21 qHTS data). This tool also allows access to the medicinal chemistry and informatics necessary to identify chemical probes that study the functions of genes, cells, and biochemical pathways. PubChem is organized as three linked databases—PubChem Substance, PubChem Compound, and PubChem BioAssaywithin NCBI's Entrez information retrieval system.
  • Tox21 Data Browser (beta version) allows users to access chemical quality-control information. This tool was developed by the National Center for Advancing Translational Sciences (NCATS).
  • EPA hosts a collection of flat files for Tox21 and ToxCast programs as well as the EPA iCSS Dashboard. These include assay and chemical descriptions, HTS data, and other related files.