ICCVAM Biennial Report 2024-2025

Daniel AB, van der Zalm AJ, Allen DG, Clippinger AJ, Kleinstreuer NC. 2025. Addendum to the prospective and retrospective evaluation of the eye irritation potential of agrochemical formulations. Research Triangle Park, NC: National Toxicology Program. https://doi.org/10.22427/NTP-NICEATM-1.

ICCVAM. 2024. Validation, Qualification, and Regulatory Acceptance of New Approach Methodologies. Research Triangle Park (NC): National Institute of Environmental Health Sciences. https://doi.org/10.22427/NICEATM-2.

ICCVAM. 2024. ICCVAM Biennial Progress Report 2022-2023. Research Triangle Park (NC): National Institute of Environmental Health Sciences. https://ntp.niehs.nih.gov/sites/default/files/2024-09/2022-2023_ICCVAMBienRpt_FD_508.pdf.

Reinke EN, Strickland JA, Truax J, Allen DG, Germolec D, Kleinstreuer NC. 2025. Report on the Validation of the Electrophilic Allergen Screening Assay (EASA) for the Identification of Potential Skin Sensitizers. Research Triangle Park, NC: National Institute of Environmental Health Sciences. https://doi.org/10.22427/NICEATM-3.

Strickland JA, Maldonado H, Farley-Dawson EA, LaPratt T, To KT, Truax JF, Reinke E, Allen DG, Germolec D, Kleinstreuer N. 2025. Evaluation of substances of regulatory interest using non-animal skin sensitization test methods. Research Triangle Park, NC: Division of Translational Toxicology. https://doi.org/10.22427/NICEATM-05.

Strickland JA, Maldonado H, LaPratt T, Truax JF, To KT, Farley-Dawson E, Reinke E, Allen DG, Germolec D, Kleinstreuer N. 2025. Evaluation of the Genomic Allergen Rapid Detection (GARD™)skin test method using substances of regulatory interest. Research Triangle Park, NC: Division of Translational Toxicology. https://doi.org/10.22427/NICEATM-06.

All Federal Register notices issued by NICEATM can be found on the NTP website at http://ntp.niehs.nih.gov/go/frn.

NIEHS. 2024. ICCVAM Biennial Progress Report: 2022-2023; availability of report. Federal Register. 89(179): 75549-75550.

NIEHS. 2024. ICCVAM Communities of Practice webinar on implementing computational approaches for regulatory safety assessments. Federal Register. 89(11): 2963-2964.

NIEHS. 2024. ICCVAM notice of public meeting; request for public input. Federal Register. 89(90): 38906-38907.

A full list of NICEATM and ICCVAM publications is available on the National Toxicology Program website.

Balls M, Bass R, Curren R, Fentem J, Goldberg A, Hartung T, Herrmann K, Kleinstreuer NC, Libowitz L, Parascandola J, Rowan A, Spielmann H, Stephens ML, Thomas RS, Tsaioun K. 2024. 60 Years of the 3Rs symposium: Lessons learned and the road ahead. ALTEX 41(2):179-201. https://doi.org/10.14573/altex.2403061.

Bean TG, Beasley VR, Berny P, Eisenreich KM, Elliott JE, Eng ML, Fuchsman PC, Johnson MS, King MD, Mateo R, Meyer CB, Salice CJ, Rattner BA. 2024. Toxicological effects assessment for wildlife in the 21st century: Review of current methods and recommendations for a path forward. Integr Environ Assess Manag 20(3):699-724. https://doi.org/10.1002/ieam.4795.

Behl M, Karmaus A, Rao M, Lane T, Harris J, Sachs C, Borrel A, Oyetade O, Unnkrishnan A, Hamm J, Hogberg HT. 2025. De-risking seizure liability: integrating adverse outcome pathways (AOPs), new approach methodologies (NAMs), and in silico approaches while highlighting knowledge gaps. Toxicol Sci 207(2):306-319. https://doi.org/10.1093/toxsci/kfaf109.

Bishop PL, Mansouri K, Eckel WP, Lowit MB, Allen D, Blankinship A, Lowit AB, Harwood DE, Johnson T, Kleinstreuer NC. 2024. Evaluation of in silico model predictions for mammalian acute oral toxicity and regulatory application in pesticide hazard and risk assessment. Reg Toxicol Pharmacol 149:105614. https://doi.org/10.1016/j.yrtph.2024.105614.

Blum J, Brull M, Hengstler JG, Dietrich DR, Gruber AJ, Dipalo M, Kraushaar U, Mangas I, Terron A, Fritsche E, Marx-Stoelting P, Hardy B, Schepky A, Escher SE, Hartung T, Landsiedel R, Odermatt A, Sachana M, Koch K, Donmez A, Masjosthusmann S, Bothe K, Schildknecht S, Beilmann M, Beltman JB, Fitzpatrick S, Mangerich A, Rehm M, Tangianu S, Zickgraf FM, Kamp H, Burger G, van de Water B, Kleinstreuer N, White A, Leist M. The long way from raw data to NAM-based information: overview on data layers and processing steps. ALTEX 42(1):167-180. https://doi.org/10.14573/altex.2412171.

Celardo I, Aschner M, Ashton RS, Carstens KE, Cediel-Ulloa A, Cöllen E, Crofton KM, Debad SJ, Dreser N, Fitzpatrick S, Fritsche E, Gutsfeld S, Hardy B, Hartung T, Hessel E, Heusinkveld H, Hogberg HT, Hsieh JH, Kanda Y, Knight GT, Knudsen T, Koch K, Kuchovska E, Mangas I, Marty MS, Melching-Kollmuss S, Müller I, Müller P, Myhre O, Paparella M, Pitzer E, Bal-Price A, Sachana M, Schlüppmann K, Shafer TJ, Schäfer J, Smirnova L, Tal T, Tanaskov Y, Tangianu S, Testa G, Ückert AK, Whelan M, Leist M. 2025. Developmental neurotoxicity (DNT): A call for implementation of new approach methodologies for regulatory purposes: Summary of the 5th International Conference on DNT Testing. ALTEX 42(2):323-349. https://doi.org/10.14573/altex.2503191.

Cöllen E, Bartmann K, Blum J, Carstens K, Celardo I, Chatterjee N, Corvaro M, Dreser N, Fritsche E, Hartung T, Hogberg HT, Knudsen T, Koch K, Kreutz A, Lislien M, Magel V, Marty SM, Pallocca G, Bal-Price A, Rovida C, Sachana M, Shafer TJ, Smirnova L, Suciu I, Tanaskov Y, Tangianu S, Wolfbeisz C, Leist M. 2025. Mapping out strategies to further develop human-relevant, new approach methodology (NAM)-based developmental neurotoxicity (DNT) testing. ALTEX 42(2):308-322. https://doi.org/10.14573/altex.2501091.

Cong G, Patton RM, Chao F, Svoboda DL, Casey WM, Schmitt CP, Murphy C, Erickson JN, Combs P, Auerbach SS. 2024. Completion of the DrugMatrix toxicogenomics database using ToxCompl. BioRxiv. https://doi.org/10.1101/2024.03.26.586669.

Daniel AB, van der Zalm AJ, Raabe HA, Clippinger AJ, Choksi NY, Reinke EN, Allen DG, Kleinstreuer NC. 2025. Defined approaches to predict GHS and EPA classifications for ocular irritation potential of agrochemical formulations. Cutan Ocular Toxicol 44(3):233-249. https://doi.org/10.1080/15569527.2025.2499552.

Daood NJ, Carey SR, Chung E, Wang T, Kreutz A, Girireddy M, Chakravarti S, Kleinstreuer NC, Tiley JB, Aleksunes LM, Zhu H. 2025. Machine learning modeling for ABC transporter efflux and inhibition: Data curation, model development, and new compound interaction predictions. Mol Pharm 22(11):7022-7035. https://doi.org/10.1021/acs.molpharmaceut.5c01065.

Debad S, Allen D, Bandele O, Bishop C, Blaylock M, Brown P, Bunger MK, Co JY, Crosby L, Daniel AB, Ferguson SS, Ford K, Gamboa da Costa G, Gilchrist KH, Grogg MW, Gwinn M, Hartung T, Hogan SP, Jeong YE, Kass GE, Kenyon E, Kleinstreuer NC, Kujala V, Lundquist P, Matheson J, McCullough SD, Melton-Celsa A, Musser S, Oh I, Oyetade OB, Patil SU, Petersen EJ, Sadrieh N, Sayes CM, Scruggs BS, Tan YM, Thelin B, Nelson MT, Tarazona JV, Wambaugh JF, Yang JY, Yu C, Fitzpatrick S. 2024. Trust your gut: Establishing confidence in gastrointestinal models - An overview of the state of the science and contexts of use. ALTEX 41(3):402-424. https://doi.org/10.14573/altex.2403261.

Debad SJ, Aungst J, Carstens K, Ferrer M, Fitzpatrick S, Fritsche E, Geng Y, Hartung T, Hogberg HT, Li R, Mangas I, Marty S, Musser S, Perron M, Rattan S, Rüegg J, Sachana M, Schenke M, Shafer TJ, Smirnova L, Talpos J, Tanguay RL, Terron A, Bandele O. 2025. State of the science on assessing developmental neurotoxicity using new approach methods. ALTEX 42(1):121-144. https://doi.org/10.14573/altex.2410231.

Do T, Kim R, Auerbach S, Casey W, Rooney A, Cong G. 2025. Cross-tissue, cross-platform prediction and validation of toxicogenomics profiles with deep learning and clustering. 2024 IEEE International Conference on Data Mining Workshops 718-725. https://doi.org/10.1109/ICDMW65004.2024.00099.

Ehrlich D, Krishna S, Kleinstreuer N. 2024. Data-driven derivation of an adverse outcome pathway linking vascular endothelial growth factor receptor (VEGFR), endocrine disruption, and atherosclerosis. ALTEX 41(4):617-632. https://doi.org/10.14573/altex.2403211.

Fadeel B, Alexander J, Antunes SC, Dalhoff K, Fritsche E, Hogberg HT, Huaux F, Oredsson S, Pietroiusti P, Svingen T, Wilks MF. 2025. Five grand challenges in toxicology (editorial). Front Toxicol 6:1533238. https://doi.org/10.3389/ftox.2024.1533238.

Fleming JF, House JS, Chappel JR, Motsinger-Reif AA, Reif DM. 2024. Guided optimization of ToxPi model weights using a semi-automated approach. Comput Toxicol 29:100294. https://doi.org/10.1016/j.comtox.2023.100294.

Foster C, Wignall J, Kovatch S, Choksi N, Allen D, Trgovcich J, Rochester JR, Ceger P, Daniel A, Hamm J, Truax J, Blake B, McIntyre B, Sutherland V, Stout MD, Kleinstreuer N. 2024. Standardizing extracted data using automated application of controlled vocabularies. Environ Health Perspect 132(2):027006. https://pmc.ncbi.nlm.nih.gov/articles/PMC10863721/.

Gao Y, Mughal Z, Jaramillo-Villegas JA, Corradi M, Borrel A, Lieberman B, Sharif S, Shaffer J, Fecho K, Chatrath A, Maertens A, Teunis MAT, Kleinstreuer N, Hartung T, Luechtefeld T. 2025. BioBricks.ai: a versioned data registry for life sciences data assets. Front Artif Intell. https://doi.org/10.3389/frai.2025.1599412.

Germolec D, Johnson V, Reinke E, Strickland J, Kleinstreuer N. 2024. Exploring applicability domain and predictive value across in vitro platforms to detect potential dermal sensitizers. Toxicol Letters 399(S2):S22. https://doi.org/10.1016/j.toxlet.2024.07.068.

Groff K, Allen D, Casey W, Clippinger AJ. 2024. Progress and remaining opportunities to increase the use of animal-free antibodies in the USA. Alternatives to Laboratory Animals 52(5):285-289. https://doi.org/10.1177/02611929241266472.

Hamm JT, Hsieh J-H, Roberts GK, Collins B, Gorospe J, Sparrow B, Walker NJ, Truong L, Tanguay RL, Dyballa S, Minana R, Schiavone V, Terriente J, Weiner A, Muriana A, Quevedo C, Ryan KR. 2024. Interlaboratory study of zebrafish in toxicology: Systematic Evaluation of the Application of Zebrafish in Toxicology's (SEAZIT's) evaluation of developmental toxicity. Toxics 12(1):93. https://doi.org/10.3390/toxics12010093.
Recognized as a "2024 Paper of the Year" by the NIEHS Environmental Factor newsletter, January 2025.

Hari A, AbdulHameed MDM, Balik-Meisner MR, Mav D, Phadke DP, Scholl EH, Shah RR, Casey W, Auerbach SS, Wallqvist A, Pannala VR. 2024. Exposure to PFAS chemicals induces sex-dependent alterations in key rate-limiting steps of lipid metabolism in liver steatosis. Front Toxicol 6:1390196. https://doi.org/10.3389/ftox.2024.1390196.

Hari A, Balik-Meisner MR, Mav D, Phadke DP, Scholl EH, Shah RR, Casey W, Auerbach, Wallqvist A, Pannala VR. 2025. Genome-scale metabolic modeling predicts per- and polyfluoroalkyl substance-mediated early perturbations in liver metabolism. Toxic 13(8):684. https://doi.org/10.3390/toxics13080684.

Hartung T, King NMP, Kleinstreuer N, Leist M, Tagle D. 2024. Leveraging biomarkers and translational medicine for preclinical safety - Lessons for advancing the validation of alternatives to animal testing (editorial). ALTEX 41(4):545-566. https://doi.org/10.14573/altex.2410011.

Hartung T, Kleinstreuer N. 2025. Challenges and opportunities for validation of AI-based new approach methods. ALTEX 42(1):3-21. https://doi.org/10.14573/altex.2412291.

Herzler M, Abedini J, Allen DG, Germolec D, Gordon J, Ko HS, Matheson J, Reinke I, Strickland J, Thierse HJ, To K, Truax J, Vanselow JT, Kleinstreuer N. 2024. Use of human predictive patch test (HPPT) data for the classification of skin sensitization hazard and potency. Arch Toxicol 98:1253-1269. https://doi.org/10.1007/s00204-023-03656-4.

Hogberg HT, Fritsche E, Gröters S, Kovi RC, Rao DB, Terron A, Winter MJ. 2025. 2024 International Academy of Toxicologic Pathology (IATP) satellite symposium: New Approach Methodologies (NAMs) for Neurotoxicity Assessment and Regulatory Perspectives. Toxicologic Pathology 53(4):305-320. https://doi.org/10.1177/01926233251335719.

Hogberg HT, Tsaioun K, Breidenbach JD, Elmore B, Filipovska J, Garcia-Reyero N, Hargreaves AJ, Joshi O, Omeragic E, Plant S, Ram R, Virmani I, Waspe J, Macmillan DS. 2024. A systematic scoping review of the neurological effects of COVID-19. NeuroToxicology 103:16-26. https://doi.org/10.1016/j.neuro.2024.05.003.

Johnson VJ, Luster MI, Edwards A, Kashon M, Burleson GR, Burleson FG, Germolec DR. 2025. An in vitro test battery using human whole blood for immunotoxicity hazard identification: Proof of concept studies with dexamethasone and benzo (a) pyrene. Toxicology 515:154153. https://doi.org/10.1016/j.tox.2025.154153.

Keebaugh AJ, Steele ML, Islas-Robles A, Phillips J, Hilberer A, Cantrell K, Chushak YG, Mattie DR, Clewell RA, Merrill EA. 2025. Development of preliminary candidate surface guidelines for air force-relevant dermal sensitizers using New Approach Methodologies. Toxics 13(8):660. https://doi.org/10.3390/toxics13080660.

Kleinstreuer N, Hartung T. 2024. Artificial intelligence (AI) – it's the end of the tox as we know it (and I feel fine)*. Arch Toxicol 98:735-754. https://link.springer.com/article/10.1007/s00204-023-03666-2.

Kreutz A, Chang X, Hogberg HT, Wetmore BA. 2024. Advancing understanding of human variability through toxicokinetic modeling, in vitro-in vivo extrapolation, and new approach methodologies. Hum Genomics 18:129. https://doi.org/10.1186/s40246-024-00691-9.

Kreutz A, Chang X, Lawless M, Proença S, Schaller S, Kleinstreuer N, Hogberg HT. 2025. Comparison of physiologically based pharmacokinetic modeling platforms for developmental neurotoxicity in vitro to in vivo extrapolation. Toxicol Sci. https://doi.org/10.1093/toxsci/kfaf147.

Kreutz A, Oyetade OB, Chang X, Hsieh JH, Behl M, Allen DG, Kleinstreuer NC, Hogberg HT. 2024. Integrated approach for testing and assessment for developmental neurotoxicity (DNT) to prioritize aromatic organophosphorus flame retardants. Toxics 12(6):437. https://doi.org/10.3390/toxics12060437.

Luo X, Zhang L, Sakamuru S, Adesina PA, Zhao J, Xia M, Xu T, Ngan DK, Travers J, Wood S, Sukhawanit R, Garrison A, Klumpp-Thomas C, Ferguson SS, Zhu H, Hall MD, Reif DM, Huang R. 2025. Systematic evaluation of Tox21 compounds that target β-adrenergic receptors and their role in cardiotoxicity. Toxicol Appl Pharmacol 505:117567. https://doi.org/10.1016/j.taap.2025.117567.

Maertens A, Kincaid B, Bridgeford E, Brochot C, Carvalho e Silva A, Dorne JCM, Geris L, Husøy T, Kleinstreuer N, Ladeira LCM, Middleton A, Reynolds J, Rodriguez B, Roggen EL, Russo G, Thayer K, Hartung T. 2025. From cellular perturbation to probabilistic risk assessments. ALTEX 42(3):413-434. https://doi.org/10.14573/altex.2501291.

Mansouri K, Moreira-Filho JT, Lowe CN, Charest N, Martin T, Tkachenko V, Judson R, Conway M, Kleinstreuer NC, Williams AJ. 2024. Free and open-source QSAR-ready workflow for automated standardization of chemical structures in support of QSAR modeling. J Cheminform 16:19. https://doi.org/10.1186/s13321-024-00814-3.

Mansouri K, Taylor K, Auerbach S, Ferguson S, Frawley R, Hsieh JH, Jahnke G, Kleinstreuer N, Mehta S, Moreira-Filho JT, Parham F, Rider C, Rooney AA, Wang A, Sutherland V. 2024. Unlocking the potential of clustering and classification approaches: navigating supervised and unsupervised chemical similarity. Environ Health Perspect 132(8):085002. https://pmc.ncbi.nlm.nih.gov/articles/PMC11302584/.
Recognized as a "2024 Paper of the Year" by the NIEHS Environmental Factor newsletter, January 2025.

Marciano LPA, Kleinstreuer N, Chang X, Costa LF, Silvério ACP, Martins I. 2024. A novel approach to triazole fungicides risk characterization: Bridging human biomonitoring and computational toxicology. Sci Total Environ 953:176003. https://doi.org/10.1016/j.scitotenv.2024.176003.

Mathisen GH, Bearth A, Jones LB, Hoffmann S, Vist GE, Ames HM, Husøy T, Svendsen C, Tsaioun K, Ashikaga T, Bloch D, Cavoski A, Chiu WA, Davies HG, Giusti A, Hartung T, Hirabayashi Y, Hogberg HT, Joglekar R, Kojima H, Krishnan K, Kwon S, Osborne OJ, Roggen E, Rooney AA, Rousselle C, Sass JB, Sepai O, Simanainen U, Thayer KA, Tong W, Wikoff D, Wright F, Whaley P. 2024. Time for CHANGE: system-level interventions for bringing forward the date of effective use of NAMs in regulatory toxicology. Arch Toxicol 98:2299–2308. https://doi.org/10.1007/s00204-024-03802-6.

Mathisen GH, Svendsen C, Vist GE, Husøy T, Ames HM, Bearth A, Audebert M, Bernard A, Beronius A, Bruzell EM, Di Consiglio E, Davenport M, Druwe I, Geci R, Gundert-Remy U, Hartung T, Hoffmann S, Hogberg HT, Hooijmans CR, Lizarraga LE, Olker JH, Prieto P, Robinson JF, Rooney AA, Sebollela A, Smith NM, Spilioti E, Spyropoulou A, Tcheremenskaia O, Testai E, Wang A, Zilliacus J, Whaley P. 2025. Identification of concepts of importance for the assessment of internal validity of in vitro toxicology studies using a modified Delphi technique. Evid Based Toxicol 3(1)2551013 https://doi.org/10.1080/2833373X.2025.2551013.

Mauge-Lewis KA, Ramaiahgari SC, Auerbach SS, Roberts GK, Waidyanatha S, Fenton SE, Phadke DP, Balik-Meisner MR, Tandon A, Mav D, Howard B, Shah R, Sparrow B, Gorospe J, Ferguson SS. 2025. Unraveling human hepatocellular responses to PFAS and aqueous film-forming foams (AFFFs) for molecular hazard prioritization and in vivo translation. Env Sci Technology 59(5):2423-2435. https://doi.org/10.1021/acs.est.4c10595.

Mengeling BJ. Ettayapuram Ramaprasad AS, Smith MT, Turkieh D, Kleinstreuer NC, Mansouri K, Durkin KA, La Merrill MA, Furlow JD. 2025. An in silico to in vivo approach identified retinoid-X receptor activating tert-butylphenols used in food contact materials. Sci Reports 15:26102. https://doi.org/10.1038/s41598-025-09244-z.

Moreira-Filho JT, Ranganath D, Conway M, Schmitt C, Kleinstreuer N, Mansouri K. 2024. Democratizing cheminformatics: interpretable chemical grouping using an automated KNIME workflow. J Cheminformatics 16:101. https://doi.org/10.1186/s13321-024-00894-1.

Moreira-Filho JT, Ranganath D, Tieghi RS, Patton R, Sutherland V, Schmitt C, Rooney AA, Fostel J, Walker VR, Saddler T, Reif D, Mansouri K, Kleinstreuer N. 2025. Automating data extraction from scientific literature and general PDF files using large language models and KNIME: an application in toxicology. WIREs Computational Molecular Science. https://doi.org/10.1002/wcms.70047.

Moyer HL, Vergara L, Stephan C, Sakolish C, Lin HC, Chiu WA, Villenave R, Hewitt P, Ferguson SS, Rusyn I. 2025. Human small intestinal tissue models to assess barrier permeability: comparative analysis of Caco-2 cells, jejunal and duodenal enteroid-Derived Cells, and EpiIntestinalTM tissues in membrane-based Cultures with and without flow. Bioengineering 12(8):809. https://doi.org/10.3390/bioengineering12080809.

Ngan DK, Sakamuru S, Zhao J, Xia M, Ferguson SS, Reif DM, Simeonov A, Huang R. 2025. Application of cytochrome P450 enzyme assays to predict p53 inducers and AChE inhibitors that require metabolic activation. Toxicol Appl Pharmacol 499:117315. https://doi.org/10.1016/j.taap.2025.117315.

Ooka M, Sakamuru S, Zhao J, Qu Y, Fang Y, Tao D, Huang R, Ferguson S, Reif D, Simeonov A, Xia M. 2024. Use of Tox21 screening data to profile PFAS bioactivities on nuclear receptors, cellular stress pathways, and cytochrome p450 enzymes. J Hazard Mater 473:134642. https://doi.org/10.1016/j.jhazmat.2024.134642.

Ooka M, Zhao J, Zhang L, Huang R, Sakamuru S, TeKrony C, Hsieh JH, Collins BJ, Dunnick JK, Dixon D, Xia M. 2025. Profiling of environmental mixtures containing metals for their toxicity pathways and mechanism of action. Environ Sci Technol 59(10):4778-4787. https://doi.org/10.1021/acs.est.4c07995.

Oyetade O, Allen DG, Carder J, Farley-Dawson EA, Reinke E, Marko S, Kleinstreuer NC, Hogberg HT. 2025. Ways to broaden the awareness, consideration and adoption of new approach methodologies (NAMs). ALTEX 42(4):714-726. https://doi.org/10.14573/altex.2505281.

Parham F, Eccles KM, Rider CV, Sakamuru S, Xia M, Huang R, Tice RR, Dinse GE, Devito MJ. 2025. Lessons learned from evaluating defined chemical mixtures in a high throughput estrogen receptor assay system. Toxicol Sci 205(1):191-204. https://doi.org/10.1093/toxsci/kfaf020.

Patlewicz G, Judson RS, Williams AJ, Butler T, Barone Jr S, Carstens KE, Cowden J, Dawson JL, Degitz SJ, Fay K, Henry TR, Lowit A, Padilla S, Paul Friedman K, Phillips MB, Turk D, Wambaugh JF, Wetmore BA, Thomas RS. 2024. Development of chemical categories for per-and polyfluoroalkyl substances (PFAS) and the proof-of-concept approach to the identification of potential candidates for tiered toxicological testing and human health assessment. Comput Toxicol 31:100327. https://doi.org/10.1016/j.comtox.2024.100327

Pradeep P, Bouhifd M, Dorne JL, Herzler M, Kleinstreuer N, Mansouri K, Pirow R, Schmeisser S, Schmitt B, Schütte K, Simanainen U. Weyrich, A, Sobanski, T. 2025. P30-38 Towards phasing out acute toxicity in vivo testing in EU chemical safety assessments. Toxicol Lett 411(Supp):S390-b. https://doi.org/10.1016/j.toxlet.2025.07.899.

Raabe HA, Costin GE, Allen DG, Lowit A, Corvaro M, O'Dell L, Breeden-Alemi J, Page K, Perron M, Flint Silva T, Westerink W, Baker E, Sullivan K. 2025. Human relevance of in vivo and in vitro skin irritation tests for hazard classification of pesticides. Cut Ocular Toxicol 44(1):1-21. https://doi.org/10.1080/15569527.2024.2387596.

Rattner BA, Bean TG, Beasley VR, Berny P, Eisenreich KM, Elliott JE, Eng ML, Fuchsman PC, King MD, Mateo R, Meyer CB, O’Brien JM, Salice CJ. 2024. Wildlife ecological risk assessment in the 21st century: Promising technologies to assess toxicological effects. Integr Environ Assess Manag 20(3):725-48. https://doi.org/10.1002/ieam.4806.

Reinke EN, Reynolds J, Gilmour N, Reynolds G, Strickland J, Germolec D, Allen DG, Maxwell G, Kleinstreuer NC. 2025. The skin allergy risk assessment-integrated chemical environment (SARA-ICE) defined approach to derive points of departure for skin sensitization. Current Research in Toxicology 8:100205. https://doi.org/10.1016/j.crtox.2024.100205.

Reyes DR, Esch MB, Ewart L, Nasiri R, Herland A, Sung K, Piergiovanni M, Lucchesi C, Shoemaker JT, Vukasinovic J, Nakae H, Hickman J, Pant K, Taylor A, Heinz N, Ashammakhi N. 2024. From animal testing to in vitro systems: advancing standardization in microphysiological systems. Lab Chip. 24(5):1076-87. https://doi.org/10.1039/D3LC00994G.

Roberts RA, Behl M, Hogberg HT, Dunayevich E, Morton M, Rockley K, Walker AL, Pierson J. 2025. Use of New Approach Methodologies (NAMs) in epilepsy research and seizure risk assessment. Drug Discov Today 31(1):104569. https://doi.org/10.1016/j.drudis.2025.104569.

Roncaglioni A, Kovarich S, Mansouri K, Tetko IV. 2025. Advancing human and environmental safety science using in silico methods (editorial). Chem Res Toxicol 38:1281-1282. https://doi.org/10.1021/acs.chemrestox.5c00293.

Sakamuru S, Ma D, Pierro JD, Baker NC, Kleinstreuer N, Cali JJ, Knudsen TB, Xia M. 2024. Development and validation of CYP26A1 inhibition assay for high-throughput screening. Biotechnology Journal 19(6):2300659. https://doi.org/10.1002/biot.202300659.

Sakamuru S, Travers J, Klumpp-Thomas C, Huang R, Witt K, Ferguson SS, Simmons SO, Reif D, Simeonov A, Xia M. 2025. Profiling the Tox21 compound library for their inhibitory effects on cytochrome P450 enzymes. Int J Mol Sci 26(11):4976. https://doi.org/10.3390/ijms26114976.

Sakolish C, Tsai HD, Lin HC, Bajaj P, Villenave R, Ferguson SS, Stanko JP, Becker RA, Hewitt P, Chiu WA, Rusyn I. 2025. Comparative analysis of proximal tubule cell sources for in vitro studies of renal proximal tubule toxicity. Biomedicines 13(3):563. https://doi.org/10.3390/biomedicines13030563.

Scheufen Tieghi R, Melo-Filho CC, Martin H, Moreira-Filho JT, LaPratt T, Allen D, Strickland J, Tropsha A, Kleinstreuer N, Muratov EN. 2025. External validation of STopTox – novel alternative method (NAM) for acute systemic and topical toxicity. Environ Health Perspect. https://pubmed.ncbi.nlm.nih.gov/40393008/.

Scheufen Tieghi R, Moreira-Filho JT, Martin HJ, Wellnitz J, Camamary Otoch M, Rath M, Tropsha A, Muratov EN, Kleinstreuer N. 2024. A novel machine learning model and a web portal for predicting the human skin sensitization effects of chemical agents. Toxics 12(11):803. https://doi.org/10.3390/toxics12110803.
Recognized as an "Intramural Paper of the Month" by the NIEHS Environmental Factor newsletter, January 2025.

Scheufen Tieghi R, Rath M, Moreira-Filho JT, Wellnitz J, Martin HJ, Gates K, Hogberg HT, Kleinstreuer N, Tropsha A, Muratov EN. 2025. DeTox: An in-silico alternative to animal testing for predicting developmental toxicity potential. Environ Health Perspect. https://pubmed.ncbi.nlm.nih.gov/40388119/.

Seal S, Mahale M, García-Ortegón M, Joshi CK, Hosseini-Gerami L, Beatson A, Greenig M, Shekhar M, Patra A, Weis C, Mehrjou A, Badré A, Paisley B, Lowe R, Singh S, Shah F, Johannesson B, Williams D, Rouquie D, Clevert DA, Schwab P, Richmond N, Nicolaou CA, Gonzalez RJ, Naven R, Schramm C, Vidler LR, Mansouri K, Walters WP, Wilk DD, Spjuth O, Carpenter AE, Bender A. 2025. Machine learning for toxicity prediction using chemical structures: Pillars for success in the real world. Chem Res Toxicol 38:759-807. https://doi.org/10.1021/acs.chemrestox.5c00033.

Simpson NE, Bourcier T, Sadrieh N. 2025. A Food and Drug Administration/Center for Drug Evaluation and Research nonclinical perspective on the use of human-induced pluripotent stem cell-derived cardiomyocyte data for cardiovascular safety assessment and regulatory decisions. Toxicol Sci 206(2):219-27. https://doi.org/10.1093/toxsci/kfaf064.

Smirnova L, Hogberg HT, Leist M, Hartung T. 2024. Revolutionizing developmental neurotoxicity testing – a journey from animal models to advanced in vitro systems. ALTEX 41(2):152-178. https://doi.org/10.14573/altex.2403281.

Stem AD, Scheufen Tieghi R, Chatzi VL, Kleinstreuer N, Valvi D, Thompson DC, Vasiliou V. 2025. Synergistic toxicity in alcohol-associated liver disease and PFAS exposure. Toxicol Sci. https://doi.org/10.1093/toxsci/kfaf110.

Stratford K, Kang JC, Healy SM, Tu A, Valerio LG. 2024. Investigative analysis of blood-brain barrier penetrating potential of electronic nicotine delivery systems (e-cigarettes) chemicals using predictive computational models. Expert Opin Drug Metab Toxicol 20(7):647-663. https://doi.org/10.1080/17425255.2024.2366385.

Stucki AO, Sauer UG, Allen DG, Kleinstreuer NC, Perron MM, Yozzo KL, Lowit AB, Clippinger AJ. 2024. Differences in the anatomy and physiology of the human and rat respiratory tracts and impact on toxicological assessments. Reg Toxicol Pharmacol 150:105648. https://doi.org/10.1016/j.yrtph.2024.105648.

Sunderic K, Wright AM, Kleinstreuer N, Ledbetter V, Milora KA, Happel C, Shaughnessy D, Johnstone CP, Casco DA, Macioce N, Marzec J, Kano K, Ochocinska MJ, Tagle DA. 2025. Complement-ARIE: Catalyzing the development and adoption of new approach methodologies. NAM J 1:100026. https://doi.org/10.1016/j.namjnl.2025.100026.

Tieghi RS, Høeg TB, Makary MA, Kleinstreuer N. 2025. The promise of animal testing alternatives at the US Food and Drug Administration and National Institutes of Health. NEJM AI 3(1):AIpc2500587. https://doi.org/10.1056/AIpc2500587.

Tieghi R, Rath M, Moreira-Filho J, Wellnitz J, Martin H-J, Gates K, Hogberg-Durdock H, Kleinstreuer N, Tropsha A, Muratov E. 2024. PregPred: an in-silico alternative to animal testing for predicting developmental toxicity potential. ChemRxiv. https://doi.org/10.26434/chemrxiv-2024-b0m7w.

To KT, Strickland J, Reinke E, Borrel A, Truax J, Maldonado H, Allen D, Kleinstreuer N. 2024. Computational application of internationally harmonized defined approaches to skin sensitization: DASS App. BMC Bioinformatics 25:4. https://doi.org/10.1186/s12859-023-05617-1.

Wambaugh JF, Paul Friedman K, Beal MA, Moffat I, Hughes MF, Nong A, Dorne JCM, Ashraf MW, Barton-Maclaren TS, DeVito M, Ferguson SS, Judson RS, Long AS, Paini A, Sampani S, Thomas RS, Wetmore BA. 2025. Applying new approach methods for toxicokinetics for chemical risk assessment. Chem Res Toxicol 38:1408-1441. https://doi.org/10.1021/acs.chemrestox.5c00161.

Wambaugh JF, Sipes NS, Mercado GP, Arnot JA, Bertato L, Brown TN, Chirico N, Cook C, Dawson DE, Davidson-Fritz SE, Ferguson SS, Goldsmith MR, Grulke CM, Judson RS, Mansouri K, Patlewicz G, Papa E, Pradeep P, Sangion A, Sayre RR, Thomas RS, Tornero-Velez R, Wetmore BA, Devito MJ. 2025. Collaborative evaluation of in silico predictions for high throughput toxicokinetics. Toxicol In Vitro 110:106150. https://doi.org/10.1016/j.tiv.2025.106150.

Wei Z, Xu T, Strickland J, Zhang L, Fang Y, Tao D, Simeonov A, Huang R, Kleinstreuer NC, Xia M. 2024. Use of in vitro methods combined with in silico analysis to identify potential skin sensitizers in the Tox21 10K compound library. Front Toxicol 6:1321857. https://doi.org/10.3389/ftox.2024.1321857.

Willett C, Pallocca G, Carusi A, Alépée N, Browne P, Darracq-Ghitalla-Ciok M, Grimm FA, Ingram J, Holden L, Mansouri K, Rabbe H, Roper C, Santizo K, Schmitt BG, Xiang L, Ward TA, Zhou B. 2025. The Decision Tree approach as a strategy for the global phase out of animal testing for acute and local toxicity for chemicals: recommendations from an expert workshop. Regul Toxicol Pharmacol. 164:105969. https://doi.org/10.1016/j.yrtph.2025.105969.

Yao J, Peretz J, Bebenek I, Avila A, Alapatt T, Lee B, Patel D, Brown P, Davis-Bruno K. 2025. FDA/CDER/OND experience with New Approach Methodologies (NAMs). Int J Toxicol 45(2):136-56. https://doi.org/10.1177/10915818251384270.

Yang S, Carstens KE, Ipaye I, Chen X, Hogberg HT, Kleinstreuer N, Knudsen TB, Xia M. 2025. High-throughput cytokine detection platform for evaluation of chemical induced microglial activation. SLAS Technology 35:100347. https://doi.org/10.1016/j.slast.2025.100347.

Yang S, Zhang L, Khan K, Travers J, Huang R, Jovanovic VM, Veeramachaneni R, Sakamuru S, Tristan CA, Davis EE, Klumpp-Thomas C, Witt KL, Simeonov A, Shaw MD, Xia M. 2024. Identification of environmental compounds that may trigger early female puberty by activating human GnRHR and KISS1R. Endocrinology. 165(10):bqae103. https://doi.org/10.1210/endocr/bqae103.

A searchable list of NICEATM and ICCVAM abstracts is available on the National Toxicology Program website.

Allen DG, Hull V, Reinke EN, Daniel AB, To KT, Karmaus AL, Mansouri K, Kleinstreuer NC. 2024. Curation and characterization of a reference rat acute inhalation toxicity database [Abstract 4249]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Bancos S. 2024. Biocompatibility assessment of ophthalmic medical devices [Abstract 1190]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Bhandari S, Biswas K, Bello A, Ross M, Wang C, Liu P, HewaSahabanduge C, Stueckle T, Bello D. 2025. Characterization of PFAS-containing aqueous film-forming foams and their thermal degradation products: Implication for human and environmental health [Abstract 3457]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Bodas M, Guppi S, Mandler K, McKinney W, Knepp AK, Keeley S, Battelli L, Qian Y, Stueckle TA. 2025. Whole body inhalation of polycarbonate emissions generated during 3D-printing process alters metabolism and redox status in rat liver [Abstract 3477]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Borrel A, Tedla G, To KT, Hill B, Karmaus AL, Wang A, Luechtefeld T, Reif D, Allen DG, Kleinstreuer NC. 2024. Modern in vitro imputation modeling and machine learning to predict chemical carcinogenicity [Abstract 2013]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Borrel A. 2024. Deep-learning profile QSAR modeling to impute in vitro assay results and predict chemical carcinogenesis mechanisms [Abstract 1088]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Borrel A, Cuomo D, Hill B, Reisfeld B, Karmaus A, Osborne G, Feshuk M, Blake B, Druwe I, Wang A, Bisson W, Madia F, Facchin C, De Conti A, Rigutto G, McHale C, Smith M, Chiu WA, Kleinstreuer N. 2024. Mapping Tox21/ToxCast HTS data to key characteristics of carcinogens [Abstract 75]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024.

Burns ES, Olgun NS, Friend SA, McKinney WG, Sinsel EW, Reynolds JS, Leonard SS. 2024. Peracetic acid effects on human bronchial cells in an air liquid interface [Abstract 4308]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Camacho JA, Santillo MF, Patton G, Kabadi SV. 2025. Consideration of dermal studies for safety evaluation of food contact substance (FCS) impurities, triethanolamine (TEA), trimethylolpropane triacrylate (TMPTA), and diethanolamine (DEA) [Abstract 3181]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Carstens K. 2025. New approach methods for evaluation of developmental neurotoxicity: Fit-for-purpose analysis approaches in a multidimensional data landscape [Abstract 1207]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Casey W. 2025. Regulatory challenges and opportunities in assessing metabolic competence of in vitro systems [Abstract 1175]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Casey W. 2025. Translating experience into progress: advancing regulatory acceptance of NGRA [Abstract 747]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Casey W. 2025. Leveraging strategic funding and public-private partnerships to establish confidence in NAMs for biomedical research and regulatory testing [Abstract 746]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Chang X, Palmer J, Donley E, Reinke E, Allen D, Kleinstreuer N. 2024. IVIVE to facilitate animal-free risk assessment of potential developmental toxicants [Abstract 4425]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Chang X. 2024. In vitro to in vivo extrapolation application to promote the use of NAMs for assessing inhalation toxicity [Abstract 1303]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Chen T, Bekkala A, Yan J, Chen Y, Pan B, You L, Johnson F, Inselman A, Tyler T, Zhou T. 2024. Evaluation of reproductive, developmental, and genetic toxicities of eugenol using Caenorhabditis elegans model [Abstract 3759]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Chen X, Sukhawanit R, Wood S, Sakamuru S, Yang S, Huang R, Klumpp-Thomas C, Simeonov A, Xia M. 2025. Identifying Wnt/β-catenin signaling modulators using a quantitative high-throughput screen [Abstract 3534]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Chen Y, Salazar K, Mansouri K, Kleinstreuer N. 2025. A machine learning approach for predicting inhalation acute toxicity (LC50) values of chemicals using a two-stage regression model [Abstract 5215]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Conley J, Harrill J, Carstens K, Shafer T. 2024. Development of a chemically induced long-term potentiation assay for developmental neurotoxicity screening [Abstract 161]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024.

Crittenden P, Markley LC, Patton G. 2024. Postmarket safety evaluation of the food packaging impurity trimethylolpropane [Abstract 4514]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Cuomo D, Karmaus A, Hill B, Feshuk M, Osborne G, Blake B, Druwe I, Wang A, Rigutto G, McHale C, Smith MT, Facchin C, De Conti A, Madia F, Kleinstreuer N, Chiu WA, Borrel A. 2025. Mapping Tox21/ToxCast high-throughput screening assay endpoints to key characteristics of carcinogens [Abstract 4698]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Daniel A, Casey W, Chen T, Crizer D, Ferguson S, Gordon J, Hamm J, Magurany K, Morefield S, Reinke E, Sprankle C, Fitzpatrick S, Kleinstreuer N. 2025. Validation powerlifting: strengthening confidence in NAMs with flexibility and fitness for purpose [Abstract 155]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Daniel A, van der Zalm A, Raabe H, Clippinger A, Reinke E, Allen D, Kleinstreuer N. 2024. Defined approaches for predicting GHS and EPA eye irritation classification of agrochemicals [Abstract 80]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. Winner, Outstanding Poster Presentation Award.

Daniel AB, van der Zalm AJ, Raabe HA, Clippinger AJ, Kleinstreuer NC, Allen DG. 2024. Predicting GHS hazard classification for eye irritation/corrosion potential of agrochemical formulations using defined approaches [Abstract 3460]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

de Aguiar Marciano L, Filipe Costa L, Silva Cardoso N, de Araújo Paula F, Pupin Silvério A, Kleinstreuer N, Martins I. 2024. Risk assessment of exposure to triazole fungicides by human biomonitoring and mechanistic data [Abstract 3325]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Ehrlich D, Krishna S, Kleinstreuer N. 2025. Data-driven derivation of an adverse outcome pathway linking vascular endothelial growth factor receptor (VEGFR), endocrine disruption, and atherosclerosis [Abstract 3559]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Embry MR, Nyguen A, Ferguson S, Waidyanatha S, Lawless M, Liu Y, Krzykwa J, Mitchell CA, Liu F, Fairman K, Li M, Roe A. 2024. Contextualizing botanical constituents in silico toxicology results using ADME data [Abstract 4317]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Everett LJ, Wehmas L, Vitense K, Davidson-Fritz S, Lambert J, Cowden J, Hughes MF, Harrill A, Mutlu E, Auerbach S, DeVito M, Thomas R. 2025. An analytical framework to optimize transcriptomic dose-response modeling for use in regulatory assessments [Abstract 3250]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Ferguson S. 2024. Predicting human liver injury and botanical drug interactions with 3d liver microtissues and high-throughput transcriptomics [Abstract 1067]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Ferguson S, Mauge-Lewis KA, Ramaiahgari SC, Auerbach SS, Roberts GK, Waidyanatha S, Fenton SE, Phadke DP, Balik-Meisner MR, Tandon A, Mav D, Howard B, Shah R, Sparrow B, Gorospe J. 2025. Unraveling human hepatocellular responses to PFAS and aqueous film-forming foams (AFFFs) [Abstract 3314]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Fies W, Luh J, Snow S, Martin E, Haber L, Bradley M, Reinke E, Borrel A, Hooker E, Bevington C, Gordon J, Cho T. 2024. Class-based hazard, exposure, and dose-response assessment of polyhalogenated organophosphate flame retardants [Abstract 59]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024.

Fitzpatrick S. 2024. Regulatory considerations for application of in vitro models of the immune system to safety and risk assessment [Abstract 1119]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Geriesh S, Li L, Heyward S, Gaffney J, Cottier K, Chen M, Sadrieh N, Wang H. 2025. Hepatic micropatterned co-culture in vitro models for the evaluation of DILI [Abstract 2014]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Germolec D. 2024. Gardair testing of federal agency partners chemicals of concern. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024.

Gombar VK, Sedkyh AY, Green AJ, Ross A, Shah RR, Witt KW, Casey W. 2024. Strain-specific mutagenicity models for ICH M7 submission [Abstract 4341]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Gwinn W, Roberts G, Yao P, Stout M, Ryan K, Waidyanatha, Gupta A, Pearson S, Richey J, Moyer B, Shaw J, Mukherjee S, Sparrow B. 2024. In vitro evaluation of inhalation toxicity induced by 2,3-pentanedione vapor using a VITROCELL 48 2.0 plus exposure system and air-liquid interface (ALI) airway model [Abstract 4292]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Haber L, Bradley MA, Martin EM, Snow SJ, Robertson JM, Fies W, Borrel A, Reinke E, Bevington C, Gordon J, Cho T, Hooker E, Hatlelid K. 2025. Class-based hazard and dose-response assessment of polyhalogenated organophosphate flame retardants [Abstract 4774]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Hagan B, Patlewicz G, Shah I. 2024. Metabolic similarity in read-across prediction: a case study using graph convolutional networks to predict genotoxicity outcomes from metabolic networks [Abstract 62]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024.

Hari A, Balik-Meisner MR, Mav D, Phadke DP, Scholl EH, Shah RR, Casey W, Auerbach SS, Wallqvist A, Pannala VR. 2025. Genome-scale metabolic modeling predicts PFAS-mediated early perturbations in liver metabolism [Abstract 3306]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Harrill A. 2025. Development of a new US EPA human health assessment product using transcriptomics and short-term in vivo studies [Abstract 1023]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Harrill A. 2025. Integrating new approach methods to inform chemical safety evaluation at the US EPA [Abstract 493]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Harrill A. 2025. Opportunities to build confidence in use of NAMs for systemic toxicity [Abstract 149]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Hill B, Hamm J, Hsieh JH, Reinke EN, Hogberg HT, Ryan K. 2025. Understanding the range of phenotypic responses for the embryonic zebrafish developmental toxicity assay [Abstract 3527]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Hill B, Hamm J, Hsieh JH, Knapp B, Reinke E, Hogberg H, Ryan K. 2025. Understanding the range of phenotypic responses for the embryonic zebrafish developmental toxicity assay [Abstract 286]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Hill B, Auman JT, Unnikrishnan A, Karmaus A, Hogberg H, Reinke E, Kleinstreuer N. 2024. Incorporating ontologies into high-throughput screening assay annotations to facilitate interpretation and increase data use [Abstract 40]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024.

Hill BN, Reinke EN, Auman JT, Unnikrishnan A, Allen DG, Kleinstreuer NC. 2024. Incorporating ontologies into high throughput screening assay annotations to increase data use and interpretation [Abstract 4385]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Hogberg H. 2024. Behavioral testing in zebrafish embryo as a complementary assay to enhance the developmental neurotoxicity in vitro battery (DNT-IVB) [Abstract 1233]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Hogberg H. 2025. Integrated approaches to testing and assessment case study of mifepristone to determine developmental neurotoxic potential [Abstract 1172]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Hogberg HT, Kreutz A. 2025. Application of in vitro data and physiologically based kinetic modeling in developmental neurotoxicity hazard identification and chemical risk assessment [Abstract]. Presented at the 65th Annual Meeting of the Society for Birth Defects Research and Prevention, Denver, CO, USA, 29 June–2 July 2025.

Hogberg H, Mangas I, Sachana M. 2025. Advancing defined approaches for developmental neurotoxicity using the integrated testing and assessment framework template [Abstract 607]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Hogberg-Durdock H, Hamm J, Borrel A, Karmaus A, Lane T, Unnikrishnan A, Urbina F, Behl M. 2024. Identifying seizure-inducing biological targets: a collaborative approach using AOP networks, in vitro assays, and computational tools [Abstract 1045]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Holt L, Rattan S, Kabadi SV, Cheng T. 2024. Updating the safety assessment of oral exposure to anthraquinone and vinylidene chloride based on a review of recently published toxicity data [Abstract 4524]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Hou S, Ji C, Ruiz P. 2025. Machine learning models to predict potential thyroid hormone disruptors for chemical risk assessment [Abstract 3402]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Hsieh J, Wang Z, Nolte S, Marvel S, Hill B, Hamm J, Roberts G, Schmitt, Ryan K. 2024. SEAZIT-DIVER: a web application for integrating and visualizing inter-laboratory data on substance-induced developmental toxicity phenotypes in zebrafish embryos [Abstract 4354]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Huddleston ME, Ingram K, Jenkins M, Zaets A, Grogg M, Nelson MT. 2024. Alveolus-on-a-chip reveals distinctive soot-based toxicity biomarkers in extracellular vesicles [Abstract 4294]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Hull V. 2025. Recent advances in exposure predictions: model refinement and accessibility [Abstract]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Hull V, LaPratt T, Reinke E, Kleinstreuer N. 2025. Integrating structure-based chemical taxonomies to focus queries of NAMs data [Abstract 90]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Hull V, Hill B, Unnikrishnan A, Reinke E, Kleinstreuer N, Hogberg H. 2025. Enhancing a curated high-throughput screening pipeline to improve toxicological interpretations of in vitro data [Abstract 706]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025.

Hull V, LaPratt TR, Karmaus AL, To KT, Unnikrishnan A, Allen DG, Kleinstreuer NC. 2024. Curating chemical use categories and exposure predictions to inform chemical assessment [Abstract 4322]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Hunt PR, Ferguson M, Olejnik N, Fitzpatrick SC, Yourick J, Sprando RL. 2025. 5-fluorouracil, hydroxyurea, and ribavirin induce both irreversible and reversible developmental effects in Caenorhabditis elegans [Abstract 4819]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Jackson B, Minnema L, Wu Y, Schlosser C, Hofstra A, Carstens K, Shafer T. 2024. Utility of developmental neurotoxicity in vitro battery to address regulatory challenges [Abstract 4245]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Jackson BL, Wu Y, Schlosser C, Hofstra A, Minnema L, Fritsche E, Klose J, Shafer T, Carstens K, Karmaus A. 2025. Leveraging the developmental neurotoxicity in vitro battery to contextualize in vivo apical observations [Abstract 4650]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Jackson B, Wu Y, Schlosser C, Hofstra A, Minnema L, Shafer T, Carstens K, Karmaus A. 2024. Integrating mechanistic data to contextualize in vivo apical observations using the developmental neurotoxicity in vitro battery [Abstract 145]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024.

Ji C, Prussia AJ, Mumtaz M, Ruiz P. 2024. Toward the application of in silico tools to chemical mixtures of environmental incidences [Abstract 4320]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Ji C, Ruiz P. 2025. Serum per- and polyfluoroalkyl substances and lipids: A systematic review and meta-analysis [Abstract 4574]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Johnson VJ, Reinke EN, LaPratt TR, Strickland J, To KT, Larne O, Johansson H, Forreryd A, Allen DG, Kleinstreuer NC, Germolec DR. 2024. Testing of regulatory-relevant chemicals for skin and respiratory sensitization hazard [Abstract 4189]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Judson R, Smith D, DeVito M, Wambaugh JF, Wetmore BA, Friedman KP, Padilla S, Weitekamp C, Patlewicz G, Thomas RS, Sayre RR, Olker J, Degitz S, Padilla S, Harrill J, Shafer T, Carstens K. 2024. A comparison of in vitro and in vivo points of departure with human biomonitoring levels for per- and polyfluoroalkyl substances (PFAS) [Abstract 3569]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Kleinstreuer N. 2024. Cardiotoxicity NAMs across the translational toxicology pipeline [Abstract]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Kleinstreuer N. 2025. Building and applying high-confidence computational models in med tech [Abstract 1299]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Kleinstreuer N. 2025. Advancing one health preparedness: integrating computational dynamic and kinetic systems models into regulatory frameworks [Abstract 1325]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Kleinstreuer N. 2025. Resources to support validating NAMs for DART [Abstract]. Presented at the 65th Annual Meeting of the Society for Birth Defects Research and Prevention, Denver, CO, USA, 29 June–2 July 2025. 

Kleinstreuer N. 2025. From data to decisions: AI’s role in modern toxicology [Abstract 187]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Ko A, Vahanan MM, Carstens K, Harrell A, Paul Friedman K, Feshuk M. 2024. Generating GD211-aligned documentation for ToxCast to support assay interpretation and data use [Abstract 51]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Krajnak K, Mandler K, McKinney W, Knepp A, Jackson M, Waugh S, Chapman P, Matheson J, Thomas T, Qian Y. 2024. Effects of polycarbonate emissions generated by 3-dimensional printing on circulating steroids and anterior pituitary hormones [Abstract 3918]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Kreutz A. 2024. In vitro to in vivo extrapolation for developmental neurotoxicity: a comparison of physiologically based pharmacokinetic models [Abstract]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Kreutz A. 2025. In vitro to in vivo extrapolation for developmental neurotoxicity [Abstract 1105]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Kreutz A, Karmaus AL, Oyetade OB, Paul Friedman K, Allen DG, Reinke EN, Paparella M, Kleinstreuer NC. 2025. Quantifying the reproducibility and variability of in vivo guideline toxicology studies [Abstract 3022]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Kreutz A, Reisfeld B, Chang X, Hamm J, Ryan K, Hogberg H, Hsieh JH. 2025. Implementation of human and zebrafish physiologically based pharmacokinetic modeling for developmental neurotoxicity hazard assessment [Abstract]. Presented at the 19th Biannual Meeting of the International Neurotoxicity Association, Sommarøy, Norway, 7–12 September 2025. 

Kreutz A, Chang X, Lawless M, Shafer TJ, Wambaugh JF, Wetmore BA, Allen DG, Hogberg HT. 2024. In vitro to in vivo extrapolation for developmental neurotoxicity: a comparison of physiologically based pharmacokinetic models [Abstract 4454]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Krishna S, Chang X, Eccles K, Messier K, Kleinstreuer N. 2025. Multiscale risk characterization of chemical cardiotoxicity: integrated in vitro and in silico multiscale modeling reveals cardiovascular risk from chemical exposures [Abstract 2061]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Krishna S, Beverly B, Walker VR, Kleinstreuer NC, Hannani A, Whately H, Trgovcich J, Rochester J, Sprowles J, Miller M, Tracy W, Sibrizzi C, Rooney AA. 2024. Artificial intelligence approaches to identify and categorize health-relevant research for big questions: case study of cardiovascular disease and environmental exposures [Abstract 4392]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Kruger L, Ngan K, Xu T, Xia M, Simeonov A, Huang R. 2025. In vitro profiling of cosmetic ingredients within the Tox21 10K compound library for bioactivity and potential toxicity [Abstract 3375]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Lane TR, Snyder S, Harris JS, Hamm J, Urbina F, Unnikrishnan A, Borrel A, Karmaus A, Behl M, Hogberg-Durdock H. 2024. Seizure predictions using adverse outcome pathway-based fast interpretable greedy-tree sums modeling [Abstract 4405]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

LaPratt T, Hull V, Reinke E, Kleinstreuer N. 2024. Structure-based chemical taxonomy to focus chemical queries [Abstract 39]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Leary AJ, Patlewicz G, Shah I. 2024. An exploration of the use of hybrid fingerprints in generalized read-across and their impact on predictive performance for selected in vivo toxicity outcomes [Abstract 42]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Li X, He X, Chen S, Xu H, Guo X, Manjanatha MG, Mei N. 2025. Establishing TK6 cells co-expressing multiple human cytochrome P450s for genotoxicity testing [Abstract 4101]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Lin H, Sakolish C, Moyer HL, Ferguson SS, Stanko JP, Carmichael PL, Hewitt P, Hoffmann S, Rusyn I, Chiu WA. 2024. Predicting renal clearance of PFAS with a human kidney proximal tubule tissue chip and a novel physiologically-based kidney model [Abstract 3508]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Liu J, Rider C, Waidyanatha S, Qu W, Dunlap P, Roberts G, Ferguson S. 2025. Effects of PFAS mixtures exposure on 3D human liver microtissues: A toxicity evaluation using in vitro markers [Abstract 3944]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Londenberg A, Api AM, Boislève F, Bowden AM, Collins S, Joshi K, Moustakasb H, Muldoon J, Patlewicz G, Zocher M, Singal M, Sadekar N, Escher S. 2025. Development of TTC values for inhalable substances [Abstract 304]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Lowit A. 2024. Update on collaborations to support implementation of in vitro and computational approaches from the US EPA Office of Pollution Prevention and Toxics [Abstract 1097]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Lowit A. 2025. Leadership at the confluence of science and government [Abstract]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Lundin B, Knight G, Fedorchak N, Krucki K, Birtele M, Iyer N, Robinson J, Iskandar B, Quadrato G, Carstens K, Willett R, Ashton R. 2025. RosetteArray platform for quantitative high-throughput screening of human developmental neurotoxicity and neurodevelopmental disorder risk factors [Abstract 4663]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Luo X, Xu T, Ngan DK, Xia M, Zhao J, Sakamuru A, Simeonov A, Huang R. 2025. Prediction of chemical-induced acute toxicity using in vitro assay data and chemical structure [Abstract 3392]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Luz A, Corton C, Allran J, Beachum C, Lowit A. 2025. Establishing a non-linear cancer mode of action for di-isononyl phthalate [Abstract 4707]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Lynch C, Sakamuru S, Travers J, Zhao T, Klumpp-Thomas C, Huang R, Xia M. 2025. Human pregnane X receptor antagonist identification employing a high-throughput screen [Abstract 4501]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Mansouri K, Kleinstreuer N. 2025. The CATMoS model: an in silico tool for predicting acute oral toxicity [Abstract 101]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Mansouri K, Kleinstreuer N. 2025. Assessing the utility of the collaborative acute toxicity modeling suite (CATMoS) from ecological risk assessment to human health hazard [Abstract 102]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Mansouri K, Moreira-Filho JT, Kleinstreuer N. 2025. Understanding the concept of similarity and its applications to toxicological research and risk assessment [Abstract 100]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Markley L, Verma R, Arvidson K, Chen T, Bandele O. 2024. Mutagenicity predictions for hazard prioritization of photoinitiators used in printing inks on the exterior of food packaging [Abstract 4522]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Marko S, Allen DG, Hogberg H, Oyetade OB, Kleinstreuer NC, Carder J. 2024. Opportunities for encouraging the consideration of alternative methods [Abstract 3495]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Mav D, Ross A, Balik-Meisner M, Phadke D, Auerbach S, Merrick BA. 2024. Transcriptomic read-across for characterization of chemical mechanisms-of-action [Abstract 4447]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Maxwell G, Reynolds J, Gilmour N, Strickland J, Reinke EN, Germolec D, Truax J, Allen DG, Blaylock M, LaPratt T, Reynolds G, Kleinstreuer N. 2024. Application of skin allergy risk assessment-integrated chemical environment defined approach SARA-ICE DA to assess skin sensitization potency of isothiazolinone compounds [Abstract 3506]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Mayil Vahanan M, Shafer TJ, Paul Friedman K, Carstens KE. 2024. Evaluating two analysis methods for the microelectrode array network formation assay [Abstract 4458]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

McKim JM, Austin D, Sprando R, Hermansky S, Mattes W, Fitzpatrick S. 2024. A novel meso-scale MPS integrated organ platform to evaluate usnic acid kinetics, organ distribution and cytotoxicity: a case study [Abstract 4193]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

McPherson CA, Combs P, Cushman J, Erickson J, Hsieh J, Hall L, Hogberg HT, Kendricks K, Sills R. 2024. Integrating screening level developmental neurotoxicity (DNT) information of chemicals in a new approach methods (NAMs) battery to identify compounds for future study [Abstract 3819]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Miller SL, Chappel J, Hickman E, Koval L, Kim Y, Rebuli M, Jaspers I, Rider C, Reif D, Rager JE. 2024. Transcriptomics similarity scoring using toxicological databases to anchor in vivo wildfire smoke exposure data to human pulmonary disease signatures [Abstract 4362]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Miller SL, Hickman E, Kim YH, Rider C, Reif DM, Jaspers I, Rebuli ME, Rager JE. 2025. Characterizing individual and mixtures-based chemical contributions to wildfire smoke toxicity through in vitro transcriptomics screening [Abstract 4570]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Modaresi S, Salminen AT, Davis KJ, Felton RP, Sun J, Beland FA, Derr K, Brown PC, Ferrer M, Katz LM, Kleinstreuer NC, Leshin J, Manga P, Sadrieh N, Xia M, Fitzpatrick SC, Camacho L. 2025. Evaluation and comparison of in vitro permeation testing IVPT and barrier function in alternative skin models and excised human skin [Abstract 3123]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Moreira-Filho J, Ranganath D, Tieghi R, Patton R, Sutherland V, Schmitt C, Rooney A, Walker V, Fostel J, Saddler T, Reif D, Mansouri K, Kleinstreuer N. 2025. Automating data extraction from scientific literature and general PDF files using large language models and KNIME: an application in toxicology [Abstract 4047]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Moreira-Filho J, Ranganath D, Conway M, Waidyanatha S, Collins B, Schmitt C, Kleinstreuer N, Mansouri K. 2024. Modeling and visualization (MoVIZ): democratizing cheminformatics with a user-friendly guided pipeline [Abstract 4356]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Mutlu E, Everett L, Wehmas L, DeVito M, Harrill A, Linnenbrink M, Watkins A, Weitekamp C, Thomas R. 2025. Implementation of the new EPA Transcriptomics Assessment Product (ETAP) for data poor chemicals [Abstract 4829]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Negi CK, Sakolish S, Ferguson S, Villenave R, Hewitt P, Rusyn I. 2025. Comparative analysis of species-specific hepatocyte function and drug effects in a liver microphysiological system PhysioMimix LC12 and 96-well plates [Abstract 4504]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Ngan DK, Sakamuru A, Zhao J, Xia M, Ferguson SS, Reif DM, Simeonov A, Huang R. 2025. Application of cytochrome P450 enzyme assays to predict compounds that require metabolic activation [Abstract 3394]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Ochocinska M. 2025. Validation and qualification network (VQN) public-private partnerships for adoption and implementation of new approach methodologies (NAMs) [Abstract 287]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Oktay AB, Tandon A, Howard BE, Balik-Meisner MR, Phadke DP, Mav D, Green AJ, Pearson A, Ferguson SS, Shah RR. 2025. AI-driven 3D image analysis of liver spheroids integrated with transcriptomics for toxicology assessment [Abstract 3567]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Oktay A, Tandon A, Howard BE, Pearson A, Ferguson SS, Shah R. 2024. Nucleus and cell quantification of 3D cell painting liver spheroids with AI [Abstract 156]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Ooka M, Sakamuru S, Huang R, Ferguson S, Reif D, Simeonov A, Xia M. 2024. Comprehensive analysis of the effects of PFAS on the cellular pathways and potential toxicities [Abstract 3541]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Ooka M, Zhao J, Zhang L, Huang R, Sakamuru S, Hsieh J, Collins B, Dunnick J, Dixon D, Xia M. 2025. Profiling of environmental heavy metal compound mixtures for their carcinogenicity and mechanism of action [Abstract 3726]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Oyetade O, Kreutz A, Chang X, Hsieh JH, Behl M, Reinke E, Hogberg H. 2024. Integrated approach for testing and assessment for developmental neurotoxicity (DNT) to prioritize aromatic organophosphorus flame retardants [Abstract 55]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Oyetade OB, Chang X, Kreutz A, Hsieh JH, Behl M, Sipes N, Allen DG, Hogberg HT. 2024. OPFR prioritization using the developmental neurotoxicity in vitro test battery: an update to OECD IATA case study [Abstract 3822]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Park JK, Bandele O. 2024. Cardiovascular endpoints for food substances: scoping review [Abstract 4521]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Pearson A, Travlos G, Roberts GK, Ferguson SS. 2024. In vitro 3D human renal proximal tubule microtissues for nephrotoxicity screening studies [Abstract 2055]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Perone DM, Dickey AN, Truong L, Corliss BA, Reif DM, Wright FA, Tanguay RL. 2025. Uncovering genetic susceptibility to retene in zebrafish through genome-wide association study [Abstract 4479]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Perron M. 2025. Moving toward fit-for-purpose systematic review approaches [Abstract]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Perron M. 2025. Utilization of non-animal approaches to support pesticide assessments in the United States [Abstract 92]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Qu W, Liu J, Pearson A, Dunlap P, Waidyanatha S, Battelle B, Ferguson S. 2024. Evaluation of per- and polyfluoroalkyl substances hepatic accumulation with human 3D liver microtissues [Abstract 3550]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Qu W, Liu J, Waidyanatha S, Stiffler B, Ferguson S. 2025. Does 3D microtissue biomass alter hepatocellular modeling of per- and polyfluoroalkyl substances (PFAS) toxicity? [Abstract 3313]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Ramaiahgari S, Auerbach S, Saddler T, Waidyanatha S, Dixon D, Ferguson S. 2024. High-throughput transcriptomics (S1500+) analysis of 2D and 3D HepaRG liver cells highlight their utility as new approach methods in chemical hazard assessment [Abstract 4232]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024 

Rangananth D, Moreira-Filho JT, Mansouri K, Kleinstreuer N. 2025. Automating the generation of QSAR prediction reporting format reports using OPERA models and KNIME [Abstract 508]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Ranganath D, Moreira-Filho JT, Collins BJ, Waidyanatha S, Casey W, Kleinstreuer N, Mansouri K. 2025. Democratizing cheminformatics: case studies using the MoVIZ pipeline for automated chemical grouping and toxicological insights [Abstract 3382]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Rattan S, Alexander AK, Kabadi SV, Cheng T. 2025. Evaluating safety of a food contact substance impurity, 1,8-naphthalenediamine based on published toxicity data on a structural analog [Abstract 3134]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Reinke E. 2024. GARD™Air testing of federal agency partners chemicals of concern [Abstract]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Reinke E. 2025. Integrating NAMs for quantitative skin sensitization potency assessment into regulatory frameworks: experience from the work in OECD TG 497 [Abstract 92]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Reinke E. 2025. Ensuring transparency and rigor in the peer review of new approach methods for skin sensitization assessment [Abstract 91]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Reinke E, Reynolds E, Reynolds J, Gilmour N, Marco B, Maxwell G, Kleinstreuer N. 2025. SARA-ICE: a self-contained model for predicting a human relevant point-of-departure for skin sensitization [Abstract 508]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Reinke E, Gilmour N, Reynolds G, Reynolds J, Marco B, Borrel A, Maxwell G, Kleinstreuer N. 2024. SARA-ICE: a self-contained model for predicting a human-relevant point-of-departure for skin sensitization [Abstract 45]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Reinke E, Ulrey A, Hoffman S, Basketter D, Corsini E, Ashikaga T, Gerberick GF. 2024. Peer review for validation studies - building confidence and transparency into a new validation paradigm [Abstract 77]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Reinke EN, LaPratt T, Strickland JA, Germolec D, Truax J, Reynolds J, Reynolds G, Gilmour N, Allen DG, Maxwell G, Kleinstreuer NC. 2024. Application of the SARA-ICE skin sensitization defined approach to a diverse chemical set – a comparative case study [Abstract 4324]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024.

Reisfeld B, Borrel A, Chang X, Daniel A, Hill B, Hull V, To KT, Unnikrishnan A, Green A, McAfee E, Phillips J, Reinke E, Mansouri K, Kleinstreuer N. 2024. The Integrated Chemical Environment (ICE): advancing data availability and computational tool accessibility for the development, evaluation, and application of new approach methods [Abstract 47]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Robertson J, Snow SJ, Martin EM, Haber L, Bradley MA, Hatlelid K, Bevington C, Gordon J, Cho T, Hooker E. 2025. Comparison of noncancerous dose-response values for organohalogen flame retardants using traditional animal toxicological and NAMs data [Abstract 4830]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Rogers JD, Patlewicz G, Baker E, Bettmann A, Clippinger A, Paul Friedman K. 2024. High-throughput safety pharmacology screening to better understand data-poor chemicals [Abstract 57]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Ruiz P, Luan S, Ji C, Zarus G. 2024. Assessing the risks of environmental ototoxic chemicals on human health: a computational modeling perspective [Abstract 4368]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Sakamuru S, Sukhawanit R, Wood S, Huang R, Klumpp-Thomas C, Simeonov A, Kleinstreuer N, Knudsen TB, Xia M. 2025. Inhibitors of cytochrome P450 26A1 identified from screening the Tox21 10K compound library [Abstract 3519]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Sakamuru S, Travers J, Klumpp-Thomas C, Huang R, Witt KL, Ferguson SS, Simmons SO, Simeonov A, Xia M. 2024. Profiling the Tox21 library compounds for cytochrome P450 inhibitory activity [Abstract 3475]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Sakolish C, Moyer H, Barlow N, Roe H, Tsai H, Ford L, Bajaj P, Villenave R, Ferguson S, Stanko J, Hewitt P, Baltazar M, Rusyn I. 2025. Microphysiological systems as predictive tools for kidney nephrotoxicants: A comparison across four platforms [Abstract 3216]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Scheufen Tieghi R. 2025. DeTox: an in silico alternative to animal testing for predicting developmental toxicity potential [Abstract 1103]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Scheufen Tieghi R, Moreira-Filho J, Martin H, Wellnitz J, Otoch MC, Rath M, Tropsha A, Muratov E, Kleinstreuer N. 2025. Using ML for human skin sensitization prediction - a novel in silico tool using HPPT data [Abstract 3389]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Scheufen Tieghi R, Beasley JM, Moreira-Filho JT, Hogberg HT, Tropsha A, Kleinstreuer N, Muratov E. 2025. Elucidation of adverse outcome pathways for compound-induced developmental neurotoxicity using knowledge graphs [Abstract 87]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Scheufen Tieghi R, Rath MK, Moreira-Filho JT, Martin H, Beasley J, Hogberg HT, Kleinstreuer N, Muratov E. 2024. PregPred: a model to predict the developmental toxicity potential of environmental chemicals and FDA-approved drugs and cosmetics [Abstract 3850]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Scheufen Tieghi R, Beasley JM, Moreira-Filho JT, Hogberg HT, Tropsha A, Kleinstreuer N, Muratov E. 2025. Elucidation of adverse outcome pathways for compound-induced developmental neurotoxicity using knowledge graphs [Abstract]. Presented at the 65th Annual Meeting of the Society for Birth Defects Research and Prevention, Denver, CO, USA, 29 June–2 July 2025. 

Scheufen Tieghi R, Moreira-Filho JT, Rath M, Wellnitz J, Martin HJ, Gates K, Hogberg-Durdock HT, Kleinstreuer N, Tropsha A, Muratov EN. 2024. DeTox: an in silico alternative to animal testing for predicting developmental toxicity potential [Abstract 41]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. Winner, Suzanne Fitzpatrick Student Travel Award. 

Scheufen Tieghi R, Moreira-Filho JT, Martin HJ, Wellnitz J, Otoch C, Rath M, Tropsha A, Muratov EN, Kleinstreuer N. 2024. HuSSPred: an in-silico HPPT-based tool for human skin sensitization prediction [Abstract 62]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Schwartz-Zoche M, Api AM, Joshi K, Collins S, Patlewicz G, Bowden AM, Boisleve F, Singal M, Giusti A, Sadekar N, Escher SE. 2024. Development of a curated dataset to facilitate the derivation of inhalation threshold of toxicological concern (TTC) values [Abstract 43]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Sepehr E, Zhao Y, Vaught C, Camacho JA, Welch B, Yourick J, Sprando RL, Hunt PR. 2025. An accurate method based on ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry for the quantification of five cannabinoids in juvenile and adult Caenorhabditis elegans matrices [Abstract 3207]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Shafer TJ, Freudenrich TM, Wallace K, Carstens KE. 2024. Re-testing of false negative compounds in new approach methods (NAMs) for developmental neurotoxicity screening [Abstract 3828]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Shi Q, Ren L, Papineau KS, Sauld J, Ewart L, Avigan MI, Mendrick DL, Oliphant JH, Brown PC, Davis-Bruno KL, Frederic M, Patterson TA, Gamboa G, Schnackenberg LK, Sadrieh N. 2024. Donor-dependent responses to acetaminophen in human primary liver cells cultured in a liver microphysiological system [Abstract 4174]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Silver BB, Gerrish K, Tokar EJ. 2024. Cell-free DNA as a biomarker of toxicity and tissue-level events in cerebral organoids [Abstract 3278]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Souders CL, Rice PA, Kabadi SV. 2024. Development of a database of parameters for evaluating the biopersistence potential of substances [Abstract 4515]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Stueckle TA, Kim SH, Afshari A, Guppi S, Bunker K, Lersch T, Casuccio G, Friend S, Erdely A, Lee EG. 2025. Exposure and in vitro hazard characterization of microplastic dusts released from machining of boron nitride nanotube-enabled composites along its lifecycle [Abstract 4124]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Sullivan K, Reinke E, Macmillan DS, Raabe H, Germolec D, Allen D, Choi E, Filipovska J, Kleinstreuer N. 2024. Advancing flexibility for defined approach guidelines: developing performance standards to incorporate alternate information sources into the defined approach for skin sensitization guideline [Abstract 4576]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Sutherland V, Hogberg HT, Behl M, Hsieh J, Mansouri K, Mehta S, Roberts G, Ryan K, Taylor KW, Walker N, Walker VR, Witchey S, Rooney AA. 2024. Organophosphate flame retardants: a comprehensive toxicological assessment [Abstract 3818]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Tan C. 2025. A weight of evidence approach to inform dose setting [Abstract 1258]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Tedla G, Borrel A, Karmaus A, Hull V, To KT, Auman JT, Allen DG, Kleinstreuer N. 2024. Machine learning-based development of cytotoxicity flags within the Tox21/ToxCast assays [Abstract 4383]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Thompson JA, Stueckle TA, Boots T, Sinsel EW, McKinney W, Johnston RA. 2025. Effect of peracetic acid vapor on THP-1 macrophages in an air-liquid-interface culture [Abstract 3218]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

To KT, Borrel A, Reinke E, Kleinstreuer N. 2024. DASS App v2.0: implementing OECD guideline No. 497 updates [Abstract 54]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. Winner, Outstanding Poster Presentation Award. 

Tong ZB, Huang R, Sakamuru S, Xia M, Simeonov A, Gerhold DL. 2025. MT1-G activation for identifying chemical chelators causing cytotoxicity of human dopaminergic neurons [Abstract 4615]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Tropsha A, Muratov E, Kleinstreuer N. 2025. Systemic and topical toxicity (STOPTOX) predictor: model development and external validation [Abstract 2058]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Unnikrishnan A, To KT, Herzler M, Germolec D, Reinke E, Kleinstreuer N. 2025. Web application to classify and subcategorize skin sensitizers using human data [Abstract 3850]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Unnikrishnan A, To K, Herzler M, Germolec D, Reinke E, Kleinstreuer N. 2025. Web application to classify and subcategorize skin sensitizers using human data [Abstract 297]. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Unnikrishnan A, Chang X, Kreutz A, LaPratt T, Hogberg H, Sluka JP, Wambaugh J, Li D, Linakis M, Reinke E, Kleinstreuer N. 2025. Physiologically based pharmacokinetic PBPK modeling approach to quantify chemical distribution in brain and adipose compartments. Presented at the 13th World Congress on Alternatives and Animal Use in the Life Sciences, Rio de Janeiro, Brazil, 31 August–4 September 2025. 

Unnikrishnan A, Chang X, Kreutz A, Hull V, Tedla GE, Hogberg HT, Sluka JP, Wambaugh JF, Allen DG, Kleinstreuer N. 2024. PBPK modeling to predict chemical distribution in brain and adipose tissues [Abstract 4433]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Unnikrishnan A, To KT, Borrel A, Hull V, Daniel A, Reinke E, Kleinstreuer N. 2024. Harmonizing tox data to enhance confidence and utility [Abstract 46]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Vahanan MM, Shafer TJ, Carstens K. 2024. Comparison of data analysis approaches for a microelectrode array neural network formation assay [Abstract 70]. Presented at the 13th Annual Meeting of the American Society for Cellular and Computational Toxicology, Research Triangle Park, NC, USA, 28–30 October 2024. 

Weitekamp CA, Paul Friedman K, Auerbach S, Bandele O, Barton-Maclaren TS, Fitzpatrick S, Long AS, Lowit A, Mezencev R, Perron M, Preito P, Rasenberg M, Santillo M, Simanainen U, Smith D, Whelan M, White P, Yozzo K, Harrill AH, Thomas RS. 2025. Quantitative and qualitative concordance between clinical and nonclinical toxicity data [Abstract 2060]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Whitehead TM, Strickland J, Conduit GJ, Borrel A, Mucs D, Baskerville-Abraham I. 2024. Toxicological data gap filling of ingredients; comparison of machine learning based imputation and traditional QSAR methods [Abstract 4366]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Word L, Willis C, Padilla S, Weitekamp C, Carstens K, Everett L, Knapp B, Britton K, Judson R, Harrill J. 2024. A new approach method (NAM) to screen for the impact of endogenous stress on chemical toxicity [Abstract 3504]. Presented at the Annual Meeting of the Society of Toxicology, Salt Lake City, UT, USA, 10–14 March 2024. 

Xia M. 2025. Evaluation of PFAS effects on nuclear receptors, pathways, and cytochrome p450 enzymes using Tox21 screening data [Abstract 1202]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Xu R, Xu T, Zhao J, Xia M, Simeonov A, Huang R. 2025. Prediction of ocular toxicity using machine learning models [Abstract 3391]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Yang S, Knudsen T, Carstens K, Hogberg HT, Xia M. 2025. Development of a high-throughput assay platform for neuroinflammation detection using iPSC-derived microglia [Abstract 4629]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Yeilsey DJ, Shi Q, Schnackenberg LK, Ren L, Papineau KS, Oliphant JJ, Avigan MI, Brown PC, Davis-Bruno KL, Moulin F, Costa GG, Patterson TA, Sadrieh N. 2025. Differential toxicity of regorafenib in primary human hepatocytes maintained in sandwich culture and in a commercial liver-on-a-chip system [Abstract 4517]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025. 

Zurlinden TJ, Chiang C, Pradeep P, Patlewicz G, Judson R, Dzierlenga M, Kapraun K, Schlosser P, Christensen K, Lehmann G. 2025. Determining species-specific half-lives for all 209 PCB congeners through a Bayesian hierarchical framework and QSAR modeling [Abstract 4065]. Accepted for presentation at the Annual Meeting of the Society of Toxicology, Orlando, FL, USA, 16–20 March 2025.

Sprankle C. March 2024. NIH endorses recommendations on use of non-animal methods. Environmental Factor [cited 14 July 2026]. https://www.niehs.nih.gov/news/factor/2024/3/science-highlights/non-animal-methods.

Sprankle C. May 2024. NIEHS shares research to identify chemicals’ effects on development. Environmental Factor [cited 14 July 2026]. https://www.niehs.nih.gov/news/factor/2024/5/science-highlights/developmental-neurotox.

Sprankle C. July 2024. Public forum highlights collaborations to replace animal testing. Environmental Factor [cited 14 July 2026]. https://www.niehs.nih.gov/news/factor/2024/7/science-highlights/non-animal-methods.

Sprankle C. October 2024. News in Brief: Forum showcases new approaches to identify carcinogens. Environmental Factor [cited 14 July 2026]. https://www.niehs.nih.gov/news/factor/2024/10/beyond-the-bench/news-in-brief.

Sprankle C. November 2024. News in Brief: Panel advises federal agencies on advancing nonanimal testing methods. Environmental Factor [cited 14 July 2026]. https://www.niehs.nih.gov/news/factor/2024/11/beyond-the-bench/news-in-brief.

Sprankle C. August 2025. NIEHS leads international guideline update on nonanimal testing. Environmental Factor [cited 14 July 2026]. https://www.niehs.nih.gov/news/factor/2025/8/beyond-the-bench/chemical-toxic-potency.

Sprankle C. December 2025. Panel recognizes federal efforts to advance nonanimal testing methods. Environmental Factor [cited 14 July 2026]. https://www.niehs.nih.gov/news/factor/2025/12/science-highlights/alt-toxicological-methods.