Testing Status of Di(2-ethylhexyl) Phthalate 10188-J
CASRN: 117-81-7
Related: CAS 103-23-1
Formula: C24-H38-O4
Synonyms/Common Names
- 1,2-Benzenedicarboxylic acid, bis(2-ethylhexyl) ester
- Bis(2-ethylhexyl) phthalate
- DEHP
- Diethylhexyl phthalate
Short-Term Toxicity
- 4 days (Gavage)
(C10188C)
Completed
- Liver Targeted Testing study of Toxcast Chemicals
- Rats: Harlan Sprague-Dawley
- Dose: MR: 0, 600 mg/kg/day.
- 5 days (Gavage)
(C10188D)
Data in Review
- 5-Day Transcriptomic Dose-Response Studies
- Rats: Harlan Sprague-Dawley
- Dose: MR: 0, 8, 16, 31.25, 62.5, 125, 250, 500, or 1000 mg/kg; N=4.
- 14 days (IV Injection and Oral)
(C20526)
Completed
- Monkeys: Rhesus
- Dose: 5 mg/kg D4-DEHP in 10% intralipid -- IV: 1, 5, or 9 weeks; oral: 10 weeks of age.
- 14-Day (Dosed-Feed)
(C52733A)
Completed
- Rats: F344/N; Mice: B6C3F1
- Dose: R&M: 0, 6300, 12,500, 25,000, 50,000, 100,000 ppm. 5 per group.
- 13-Week (Dosed-Feed)
(C52733A)
Completed
- Rats: F344/N; Mice: B6C3F1
- Dose: R: 0, 1600, 25,000 ppm. M: 0, 800, 12500 ppm. 10 per group.
Long-Term Carcinogenicity
- Perinatal + 2 years (Dosed-Feed)
(C10188)
Completed
- TR-601
Toxicology and Carcinogenesis Studies of Di(2-ethylhexyl) Phthalate (CASRN 117-81-7) Administered in Feed to Sprague Dawley (Hsd:Sprague Dawley SD) Rats - Rats: Harlan Sprague-Dawley
- Carcinogenesis Results
- Male Rats Clear Evidence
- Female Rats Clear Evidence
- Dose: R: 0, 300, 1000, 3000, or 10,000 ppm; 50/sex/group.
- TR-601
- 2 years (Dosed-Feed)
(C10188B)
Completed
- TR-601
Toxicology and Carcinogenesis Studies of Di(2-ethylhexyl) Phthalate (CASRN 117-81-7) Administered in Feed to Sprague Dawley (Hsd:Sprague Dawley SD) Rats - Rats: Harlan Sprague-Dawley
- Carcinogenesis Results
- Male Rats Clear Evidence
- Female Rats Clear Evidence
- Dose: R: 0, 300, 1000, 3000, or 10,000 ppm; 50/sex/group.
- TR-601
- 2-Year (Dosed-Feed)
(C52733A)
Completed
- TR-217 (NIH Number: 82-1773)
(Peer Review Approval 10/15/1980A )
Carcinogenesis Bioassay of Di(2-ethylhexyl)phthalate (CASRN 117-81-7) in F344 Rats and B6C3F1 Mice (Feed Studies) - Rats: F344/N; Mice: B6C3F1
- Carcinogenesis Results
- Male Rats Positive
- Female Rats Positive
- Male Mice Positive
- Female Mice Positive
- Dose: R: 0, 6000, 12,000 ppm. M: 0, 3000, 6000 ppm. 50 per group.
- TR-217 (NIH Number: 82-1773)
(Peer Review Approval 10/15/1980A )
Special Studies
- Absorption Disposition Metabolism Elimination (Topical Application)
(S0054)
Completed
- Male Rats: F344/N
- Mechanisms (Gavage)
(S0678)
Completed
- Citation: Albro PW, Corbett JT, Schroeder JL, Jordan S, Matthews HB. Pharmacokinetics, interactions with macromolecules and species differences in metabolism of DEHP. Environ Health Perspect. 1982 Nov;45:19-25.Pubmed Abstract
- Rats: F344/N; Rats: Sprague Dawley
- Dose: 1.8, 18.0, 180.0 mg/kg.
- Toxicokinetic Study (Gavage; Intravenous)
(S0974)
Completed
- Male Monkeys: Rhesus
- Dose: 5 mg/kg bwt D4-DEHP. 5 mg/kg bwt D4-DEHP [2 ml/kg of a 2.5 mg/ml dosing solution].
Genetic Toxicology
- Chromosome Aberrations
(A15927)
Completed
- Mice
- Negative (Nonstandard Protocol)
- 4 days Comet Assay (Gavage)
(G10188)
Completed
- Rats: Sprague Dawley
- Drosophila
(507426)
Completed
- Citation: Zimmering, S., Mason, J.M., and Valencia, R. Chemical mutagenesis testing in Drosophila. VII. Results of 22 coded compounds tested in larval feeding experiments. Environ. Molec. Mutagen. Vol. 14 (1989) 245-252
- Reciprocal Translocation/Sex-Linked Recessive Lethal Negative
- Drosophila
(967176)
Completed
- Citation: Yoon, J.S., Mason, J.M., Valencia, R., Woodruff, R.C., and Zimmering, S. Chemical mutagenesis testing in Drosophila: IV. Results of 45 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 349-367
- Reciprocal Translocation/Sex-Linked Recessive Lethal Negative
- In Vitro Cytogenetics (CA/SCE)
(008270)
Completed
- Sister Chromatid Exchange Positive
- Chromosome Aberrations Not Tested
- In Vitro Cytogenetics (CA/SCE)
(426348)
Completed
- Sister Chromatid Exchange Weakly Positive
- Chromosome Aberrations Not Tested
- In Vitro Cytogenetics (CA/SCE)
(515010)
Completed
- Sister Chromatid Exchange Positive
- Chromosome Aberrations Not Tested
- In Vitro Cytogenetics (CA/SCE)
(587571)
Completed
- Citation: Gulati, D., Witt, K., Anderson, B., Zeiger, E.,and Shelby, M. Chromosome aberration and sister chromatid exchange test in Chinese hamster ovary cells in vitro III: Results with 27 chemicals Environ. Molec. Mutagen. Vol. 13 (1989) 133-193
- Sister Chromatid Exchange Equivocal
- Chromosome Aberrations Not Tested
- In Vitro Cytogenetics (CA/SCE)
(594940)
Completed
- Sister Chromatid Exchange Negative
- Chromosome Aberrations Weakly Positive
- In Vitro Cytogenetics (CA/SCE)
(759843)
Completed
- Sister Chromatid Exchange Negative
- Chromosome Aberrations Negative
- In Vitro Cytogenetics (CA/SCE)
(890544)
Completed
- Citation: Gulati, D., Witt, K., Anderson, B., Zeiger, E.,and Shelby, M. Chromosome aberration and sister chromatid exchange test in Chinese hamster ovary cells in vitro III: Results with 27 chemicals Environ. Molec. Mutagen. Vol. 13 (1989) 133-193
- Sister Chromatid Exchange Equivocal
- Chromosome Aberrations Not Tested
- In Vitro Cytogenetics (CA/SCE)
(923560)
Completed
- Citation: Gulati, D., Sabharwal, P., and Shelby, M. Tests for the induction of chromosomal aberrations and sister chromatid exchanges in cultured Chinese hamster ovary (CHO) cells. In Evaluation of Short-Term Tests for Carcinogens, Elsevier/North Holland 413-426
- Citation: Gulati, D., Witt, K., Anderson, B., Zeiger, E.,and Shelby, M. Chromosome aberration and sister chromatid exchange test in Chinese hamster ovary cells in vitro III: Results with 27 chemicals Environ. Molec. Mutagen. Vol. 13 (1989) 133-193
- Sister Chromatid Exchange Positive
- Chromosome Aberrations Negative
- In Vitro Cytogenetics (CA/SCE)
(978423)
Completed
- Citation: Gulati, D., Witt, K., Anderson, B., Zeiger, E.,and Shelby, M. Chromosome aberration and sister chromatid exchange test in Chinese hamster ovary cells in vitro III: Results with 27 chemicals Environ. Molec. Mutagen. Vol. 13 (1989) 133-193
- Sister Chromatid Exchange Equivocal
- Chromosome Aberrations Not Tested
- Micronucleus
(A04384)
Completed
- Mice: Tg.AC (FVB/N) Homozygous
- Male Equivocal
- Female Positive
- Micronucleus
(A15851)
Completed
- Mice: Tg.AC (FVB/N) Homozygous
- Male Negative
- Female Negative
- Micronucleus
(A15927)
Completed
- Mice: B6C3F1
- Female Equivocal
- Mouse Lymphoma
(913224)
Completed
- Citation: Myhr, B., Bower, L., and Caspary, W. Assays for the induction of gene mutations at the thymine kinase locus in L1578Y mouse lymphoma cells in culture. In Evaluation of Short-Term Tests for Carcinogens, Elsevier/North Holland Vol. (1985) 555-568
- Mice
- Negative
- Salmonella
(064176)
Completed
- Citation: Zeiger, E., Haworth, S., Mortelmans, K., and Speck, W. Mutagenicity testing of Di(2-ethylhexyl)phthalate and related chemicals in Salmonella Environ. Mutagen. Vol. 7 (1985) 213-232
- Negative
- Salmonella
(195347)
Completed
- Citation: Zeiger, E., Haworth, S., Mortelmans, K., and Speck, W. Mutagenicity testing of Di(2-ethylhexyl)phthalate and related chemicals in Salmonella Environ. Mutagen. Vol. 7 (1985) 213-232
- Negative
- Salmonella
(483211)
Completed
- Citation: Zeiger, E., Haworth, S., Mortelmans, K., and Speck, W. Mutagenicity testing of Di(2-ethylhexyl)phthalate and related chemicals in Salmonella Environ. Mutagen. Vol. 7 (1985) 213-232
- Negative
- Salmonella
(894518)
Completed
- Citation: Zeiger, E., and Haworth, S. Tests with a preincubation modification of the Salmonella/microsome assay. In Evaluation of Short-Term Tests for Carcinogens Elsevier/North Holland 187-199
- Negative
- Salmonella
(960176)
Completed
- Citation: Zeiger, E., Haworth, S., Mortelmans, K., and Speck, W. Mutagenicity testing of Di(2-ethylhexyl)phthalate and related chemicals in Salmonella Environ. Mutagen. Vol. 7 (1985) 213-232
- Negative
- Salmonella
(965968)
Completed
- Negative
Organ Systems Toxicity
- 120 day minimum Continuous Breeding (Legacy) (Dosed-Feed)
(RACB82119)
Completed
- Citation: Lamb JC 4th, Chapin RE, Teague J, Lawton AD, Reel JR. Reproductive effects of four phthalic acid esters in the mouse. Toxicol Appl Pharmacol. 1987 Apr;88(2):255-69. doi: https://doi.org/10.1016/0041-008x(87)90011-1.
- Mice: Swiss CD-1
- Dose: [Task 1] 0, .01, .025, .05%. [Task 2] 0, .01, .025, .05%. [Task 3] 0, .01, .025, .05%. [Task 4] 0, .05%.
- 0 to 20 GD Continuous Breeding (Legacy) (Dosed-Feed)
(RACB84118)
Completed
- Rats: F344/N
- Dose: [Task 1] 0, .25, .5, 1.0%. [Task 2] 0, .25, .5, 1.0%. [Task 3] 0, .25, .5, 1.0%. [Task 4] N/A.
- 3rd generation Continuous Breeding (Legacy) (Dosed-Feed)
(RACB98004)
Completed
- Citation: Blystone CR, Kissling GE, Bishop JB, Chapin RE, Wolfe GW, Foster PM. Determination of the di-(2-ethylhexyl) phthalate NOAEL for reproductive development in the rat: importance of the retention of extra animals to adulthood. Toxicol Sci. 2010 Aug;116(2):640-6. doi: https://doi.org/10.1093/toxsci/kfq147. Epub 2010 May 19.
- Citation: National Toxicology Program (NTP). 2005. Diethylhexylphthalate (CASRN 117-81-7): Multigenerational reproductive assessment by continuous breeding when administered to Sprague-Dawley rats in the diet. Research Triangle Park, NC: National Toxicology Program. RACB Report 98004. https://ntp.niehs.nih.gov/go/racb98004abs
- Male and Female Rats: Sprague Dawley
- Dose: [Control] (1.5), 10, 30, 100, 300, 1000, 7500, OR 10,000 ppm.
- 1 to 17 GD Continuous Breeding (Legacy) (Dosed-Feed)
(T0086E)
Completed
- Female Mice: Swiss
- Dose: 0%, 0.010%, 0.025%, 0.050%.
- 0 to 20 GD Conventional Teratology (Dosed-Feed)
(TER84063)
Completed
- Female Rats: F344/N
- Rats Maternal Positive
- Rats Fetal Positive
- Dose: 0.5, 1.0, 1.5, 2.0 %.
- 1 to 17 GD Conventional Teratology (Dosed-Feed)
(TER84064)
Completed
- Female Mice: Swiss
- Mice Maternal Positive
- Mice Fetal Positive
- Dose: 0.025, 0.05, 0.10, 0.15 %.
- 7 to 14 GD Short-Term in vivo Repro. Tox. (Gavage)
(T0086F)
Completed
- Citation: Screening of Priority Chemicals for Potential Reproductive Hazard
- Female Mice: Swiss
- Dose: 9860 mg (10 ml undiluted)/kg body wt.
- Utero-Lactational (Gavage)
(R10188)
- Mice: B6C3F1
- Utero-Lactational (Gavage)
(R10188)
Completed
- Mice: NIH Swiss
Toxicogenomics
- 4 days Microarray Analysis (Gavage)
(T10188)
Completed
- Liver Targeted Testing study of Toxcast Chemicals
- Rats: Harlan Sprague-Dawley
- Dose: not provided; 6 rats.
- 5 days Transcriptome Sequencing (Gavage)
(T10188B)
Data in Review
- 5-Day Transcriptomic Dose-Response Studies
- Rats: Harlan Sprague-Dawley