TR 386 Mouse Pathology Tables
INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
TETRANITROMETHANE
NTP Experiment-Test: 05072-02 Report: PEIRPT03
Study Type: CHRONIC Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
Facility: Midwest Research Institute
Chemical CAS #: 509-14-8
Lock Date: None
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50
Early Deaths
Natural Death 5 4 10
Accidently Killed 2 1
Moribund Sacrifice 12 17 16
Survivors
Terminal Sacrifice 30 27 23
Natural Death 1
Moribund Sacrifice 1 1
Animals Examined Microscopically 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (42) (40) (36)
Infiltration Cellular, Multifocal, Lymphocyte 1 (3%)
Intestine Large, Cecum (47) (44) (45)
Peyer's Patch, Hyperplasia, Lymphoid 1 (2%) 1 (2%) 1 (2%)
Intestine Large, Colon (49) (47) (46)
Infiltration Cellular, Multifocal, Lymphocyte 1 (2%)
Intestine Small, Jejunum (45) (46) (44)
Inflammation, Focal, Granulomatous 1 (2%)
Liver (49) (50) (50)
Basophilic Focus 1 (2%) 1 (2%)
Cytomegaly 1 (2%)
Eosinophilic Focus 3 (6%)
Fatty Change, Focal 1 (2%)
Granuloma, Multifocal 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation, Multifocal 1 (2%) 10 (20%) 7 (14%)
Infiltration Cellular, Focal, Lymphocyte 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 4 (8%) 2 (4%) 6 (12%)
Inflammation, Acute, Multifocal 1 (2%)
Inflammation, Multifocal, Subacute 4 (8%) 3 (6%)
Necrosis, Acute, Multifocal 3 (6%) 4 (8%) 3 (6%)
Necrosis, Chronic, Multifocal 1 (2%)
Necrosis, Focal, Subacute 2 (4%) 2 (4%)
Vacuolization Cytoplasmic, Focal 1 (2%)
Vacuolization Cytoplasmic, Multifocal 1 (2%)
Bile Duct, Hyperplasia, Multifocal 1 (2%)
Centrilobular, Fatty Change, Diffuse 1 (2%)
Centrilobular, Necrosis, Acute, Diffuse 1 (2%)
Periportal, Vacuolization Cytoplasmic,
Diffuse 1 (2%)
Portal, Inflammation, Chronic, Multifocal 2 (4%)
Right Lateral Lobe, Hepatodiaphragmatic
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Nodule 1 (2%) 1 (2%)
Vein, Thrombosis 1 (2%)
Mesentery (3) (1) (5)
Infiltration Cellular, Multifocal, Lymphocyte 3 (100%) 1 (20%)
Inflammation, Suppurative 1 (20%)
Pancreas (49) (50) (50)
Abscess, Multiple 1 (2%)
Cyst 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 10 (20%) 9 (18%) 7 (14%)
Acinus, Atrophy, Diffuse 1 (2%) 1 (2%)
Acinus, Atrophy, Multifocal 1 (2%)
Salivary Glands (48) (50) (50)
Infiltration Cellular, Focal, Lymphocyte 1 (2%) 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 26 (54%) 17 (34%) 19 (38%)
Stomach, Forestomach (48) (50) (49)
Abscess 1 (2%)
Epithelium, Hyperplasia, Diffuse 1 (2%)
Epithelium, Hyperplasia, Focal 2 (4%)
Epithelium, Hyperplasia, Multifocal 1 (2%)
Stomach, Glandular (47) (49) (49)
Infiltration Cellular, Focal, Lymphocyte 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 1 (2%) 1 (2%)
Necrosis, Acute, Multifocal 1 (2%)
Mucosa, Degeneration, Multifocal 1 (2%)
Tooth (2) (2)
Abscess 1 (50%)
Developmental Malformation 1 (50%)
Inflammation, Chronic Active 1 (50%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (2)
Aorta, Inflammation, Chronic Active, Focal 1 (50%)
Artery, Inflammation, Chronic, Multifocal 1 (50%)
Heart (47) (50) (50)
Cardiomyopathy 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 1 (2%) 1 (2%)
Inflammation, Acute, Multifocal 1 (2%)
Inflammation, Chronic, Multifocal 1 (2%)
Mineralization, Multifocal 1 (2%)
Necrosis, Acute, Focal 1 (2%)
Pigmentation 1 (2%)
Artery, Mineralization, Multifocal 1 (2%)
Atrium Left, Thrombosis 1 (2%) 1 (2%)
Epicardium, Inflammation, Chronic, Focal 1 (2%)
Epicardium, Inflammation, Chronic Active,
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM - CONT
Focal 1 (2%) 1 (2%)
Epicardium, Inflammation, Suppurative 1 (2%)
Mitral Valve, Bacterium 2 (4%)
Mitral Valve, Inflammation, Chronic Active 2 (4%)
Valve, Pigmentation 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland (49) (49) (50)
Capsule, Accessory Adrenal Cortical Nodule 1 (2%) 2 (4%) 1 (2%)
Capsule, Accessory Adrenal Cortical Nodule,
Multiple 1 (2%)
Capsule, Inflammation, Chronic Active,
Suppurative 1 (2%)
Subcapsular, Hyperplasia, Diffuse 4 (8%) 4 (8%) 4 (8%)
Subcapsular, Hyperplasia, Focal 1 (2%) 1 (2%)
Subcapsular, Hyperplasia, Multifocal 43 (88%) 43 (88%) 43 (86%)
Adrenal Gland, Cortex (49) (48) (50)
Cyst 1 (2%)
Hemorrhage, Acute 1 (2%)
Hyperplasia, Focal 1 (2%) 1 (2%)
Hyperplasia, Multifocal 1 (2%)
Adrenal Gland, Medulla (48) (46) (47)
Hyperplasia, Focal 2 (4%) 2 (4%)
Islets, Pancreatic (49) (50) (49)
Hyperplasia, Focal 3 (6%) 3 (6%)
Hyperplasia, Multifocal 10 (20%) 2 (4%) 3 (6%)
Hypoplasia 1 (2%)
Pituitary Gland (49) (49) (48)
Congestion 1 (2%)
Cyst 2 (4%) 1 (2%)
Pars Distalis, Angiectasis 8 (16%) 7 (14%) 3 (6%)
Pars Distalis, Hyperplasia 1 (2%)
Pars Distalis, Hyperplasia, Focal 4 (8%) 4 (8%) 4 (8%)
Pars Distalis, Hyperplasia, Multifocal 2 (4%)
Pars Distalis, Hypertrophy, Focal 5 (10%) 1 (2%)
Pars Distalis, Hypertrophy, Multifocal 1 (2%)
Thyroid Gland (48) (50) (50)
Cyst 2 (4%) 1 (2%)
Infiltration Cellular, Focal, Lymphocyte 1 (2%) 2 (4%)
Infiltration Cellular, Multifocal, Lymphocyte 3 (6%) 1 (2%)
Inflammation, Acute, Focal 1 (2%) 1 (2%)
Inflammation, Chronic, Focal 1 (2%) 2 (4%)
Inflammation, Chronic, Multifocal 4 (8%) 2 (4%) 2 (4%)
Inflammation, Chronic Active, Focal 1 (2%) 2 (4%) 1 (2%)
Inflammation, Chronic Active, Multifocal 2 (4%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
C-Cell, Hyperplasia, Focal 1 (2%)
Follicle, Cyst 1 (2%)
Follicle, Cyst, Multiple 1 (2%) 2 (4%) 3 (6%)
Follicular Cell, Hyperplasia, Diffuse 1 (2%)
Follicular Cell, Hyperplasia, Focal 1 (2%) 3 (6%) 2 (4%)
Follicular Cell, Hyperplasia, Multifocal 7 (15%) 9 (18%) 5 (10%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (2) (1) (1)
Atrophy 1 (50%)
Cyst 1 (100%)
Cyst, Multiple 1 (50%) 1 (100%)
Ovary (48) (49) (50)
Abscess 1 (2%)
Angiectasis, Focal 1 (2%)
Cyst 8 (17%) 8 (16%) 9 (18%)
Metaplasia, Focal, Osseous 1 (2%)
Mineralization 1 (2%)
Pigmentation 1 (2%) 1 (2%)
Thrombosis 1 (2%)
Interstitium, Hyperplasia 1 (2%)
Periovarian Tissue, Infiltration Cellular,
Lymphocyte 3 (6%) 1 (2%) 2 (4%)
Periovarian Tissue, Inflammation, Chronic
Active 1 (2%)
Uterus (49) (50) (50)
Abscess 1 (2%)
Abscess, Multiple 1 (2%)
Adenomyosis 1 (2%)
Amyloid Deposition 1 (2%)
Angiectasis, Multifocal 1 (2%)
Cyst 1 (2%) 3 (6%)
Dilatation 2 (4%) 1 (2%)
Hyperplasia, Atypical, Focal, Glandular 1 (2%)
Hyperplasia, Cystic 1 (2%) 3 (6%) 3 (6%)
Infiltration Cellular, Multifocal, Lymphocyte 1 (2%)
Inflammation, Chronic Active 1 (2%)
Thrombosis 1 (2%)
Thrombosis, Multiple 1 (2%)
Bilateral, Hyperplasia, Cystic 33 (67%) 40 (80%) 35 (70%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Epithelium, Hyperplasia, Focal 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (48) (50) (50)
Atrophy, Diffuse 1 (2%)
Atrophy, Focal 1 (2%)
Myeloid Cell, Hyperplasia 1 (2%) 3 (6%)
Lymph Node (50) (50) (50)
Hemorrhage, Acute 1 (2%)
Inguinal, Hyperplasia, Lymphoid 2 (4%) 3 (6%)
Lumbar, Hyperplasia, Lymphoid 1 (2%)
Mediastinal, Hyperplasia 1 (2%) 1 (2%)
Mediastinal, Hyperplasia, Lymphoid 1 (2%) 1 (2%) 4 (8%)
Pancreatic, Hemorrhage, Acute 1 (2%)
Renal, Hematopoietic Cell Proliferation 1 (2%)
Renal, Hemorrhage, Subacute 1 (2%)
Renal, Hyperplasia, Lymphoid 1 (2%)
Lymph Node, Mandibular (48) (43) (45)
Cyst 3 (7%)
Hemorrhage, Acute 2 (5%)
Hemorrhage, Subacute 1 (2%) 1 (2%)
Hyperplasia 1 (2%) 1 (2%)
Hyperplasia, Lymphoid 6 (13%) 6 (14%) 7 (16%)
Hyperplasia, Re Cell 1 (2%)
Infiltration Cellular, Histiocyte 1 (2%)
Inflammation, Subacute 1 (2%)
Pigmentation, Hemosiderin 1 (2%) 2 (4%)
Lymph Node, Mesenteric (41) (36) (37)
Angiectasis 1 (2%)
Depletion Lymphoid 1 (2%) 1 (3%)
Hematopoietic Cell Proliferation 4 (10%) 4 (11%) 5 (14%)
Hemorrhage, Acute 10 (24%) 4 (11%) 9 (24%)
Hemorrhage, Subacute 2 (5%) 1 (3%)
Hyperplasia, Lymphoid 1 (2%) 3 (8%)
Spleen (48) (49) (50)
Ectasia, Focal 1 (2%)
Edema 1 (2%)
Hematopoietic Cell Proliferation 10 (21%) 9 (18%) 14 (28%)
Hyperplasia, Lymphoid 2 (4%) 5 (10%) 5 (10%)
Infarct 1 (2%)
Pigmentation, Hemosiderin 1 (2%) 2 (4%)
Capsule, Ectopic Tissue 1 (2%)
Capsule, Inflammation, Chronic Active 1 (2%)
Thymus (48) (44) (32)
Angiectasis, Focal 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Depletion Lymphoid 4 (8%) 6 (14%) 6 (19%)
Ectopic Parathyroid Gland 3 (6%) 1 (2%) 2 (6%)
Hyperplasia, Lymphoid 3 (6%) 6 (14%) 4 (13%)
Metaplasia, Focal, Osseous 1 (3%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (48) (46) (45)
Ectasia, Multifocal 8 (17%) 4 (9%) 2 (4%)
Hyperplasia, Diffuse 7 (15%) 8 (17%) 6 (13%)
Inflammation, Chronic Active, Multifocal 1 (2%)
Skin (48) (46) (50)
Inflammation, Chronic Active, Focal 1 (2%)
Inflammation, Chronic Active, Multifocal 1 (2%)
Hair Follicle, Inflammation, Chronic Active 1 (2%)
Subcutaneous Tissue, Edema 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic
Active 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (49) (50) (50)
Fibrous Osteodystrophy 12 (24%) 10 (20%) 10 (20%)
Osteoporosis, Focal 1 (2%)
Skeletal Muscle (2) (1)
Inflammation, Chronic, Focal 1 (50%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (49) (50) (50)
Abscess 1 (2%)
Compression 9 (18%) 2 (4%) 8 (16%)
Hemorrhage, Multifocal 1 (2%)
Hydrocephalus 2 (4%) 1 (2%)
Mineralization, Multifocal 26 (53%) 26 (52%) 20 (40%)
Meninges, Infiltration Cellular, Multifocal,
Lymphocyte 1 (2%)
Perivascular, Infiltration Cellular,
Multifocal, Lymphocyte 2 (4%) 3 (6%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (46) (45) (47)
Exudate 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 2 (4%) 1 (2%)
Mineralization 1 (2%)
Lung (49) (50) (50)
Embolus Tumor, Multiple 1 (2%)
Hemorrhage, Acute, Focal 1 (2%)
Hemorrhage, Acute, Multifocal 1 (2%)
Hemorrhage, Multifocal, Subacute 1 (2%)
Infiltration Cellular, Diffuse, Lymphocyte 1 (2%)
Infiltration Cellular, Focal, Lymphocyte 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 16 (33%) 2 (4%) 2 (4%)
Inflammation, Acute, Focal 1 (2%)
Pigmentation, Diffuse, Hemosiderin 1 (2%)
Alveolar Epithelium, Hyperplasia, Focal 2 (4%) 13 (26%)
Alveolar Epithelium, Hyperplasia, Multifocal 7 (14%) 41 (82%)
Alveolus, Infiltration Cellular, Diffuse,
Histiocyte 1 (2%) 2 (4%)
Alveolus, Infiltration Cellular, Focal,
Histiocyte 1 (2%)
Alveolus, Infiltration Cellular, Multifocal,
Histiocyte 1 (2%) 8 (16%) 32 (64%)
Bronchiole, Hyperplasia, Focal 2 (4%)
Bronchiole, Hyperplasia, Multifocal 5 (10%) 41 (82%)
Interstitium, Inflammation, Chronic Active,
Focal 1 (2%)
Mediastinum, Infiltration Cellular,
Multifocal, Lymphocyte 2 (4%)
Mediastinum, Inflammation, Chronic 1 (2%)
Pleura, Hyperplasia 1 (2%)
Pleura, Infiltration Cellular, Lymphocyte 2 (4%)
Nose (49) (50) (50)
Lumen, Exudate 3 (6%) 30 (60%) 33 (66%)
Lumen, Foreign Body 1 (2%)
Mucosa, Inflammation, Chronic 2 (4%) 1 (2%)
Mucosa, Inflammation, Chronic Active 9 (18%) 10 (20%) 23 (46%)
Mucosa, Ulcer 1 (2%) 2 (4%)
Nasolacrimal Duct, Exudate 1 (2%)
Nasolacrimal Duct, Inflammation, Chronic 1 (2%)
Nasolacrimal Duct, Inflammation, Chronic
Active 1 (2%) 1 (2%)
Olfactory Epithelium, Atrophy 2 (4%) 4 (8%) 2 (4%)
Respiratory Epithelium, Hyperplasia 2 (4%) 5 (10%) 17 (34%)
Respiratory Epithelium, Metaplasia, Squamous 2 (4%) 8 (16%)
Sinus, Hyperplasia 1 (2%)
Sinus, Inflammation, Chronic Active 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Trachea (50) (50) (50)
Inflammation, Chronic 2 (4%) 2 (4%)
Inflammation, Chronic Active 2 (4%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (2)
Abscess 1 (50%)
Atrophy 1 (50%)
Harderian Gland (1) (1) (1)
Inflammation, Chronic Active 1 (100%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (49) (50) (50)
Cytoplasmic Alteration, Multifocal 1 (2%)
Infarct 3 (6%)
Infiltration Cellular, Lymphocyte 20 (41%) 14 (28%) 15 (30%)
Inflammation, Acute, Multifocal 1 (2%)
Metaplasia, Focal, Osseous 1 (2%) 1 (2%)
Nephropathy, Chronic 2 (4%)
Pigmentation, Diffuse 1 (2%)
Cortex, Mineralization, Multifocal 1 (2%)
Interstitial Tissue, Inflammation, Chronic,
Multifocal 2 (4%)
Renal Tubule, Hyperplasia, Focal 1 (2%)
Renal Tubule, Necrosis, Acute, Multifocal 1 (2%)
Urinary Bladder (48) (49) (47)
Infiltration Cellular, Focal, Lymphocyte 3 (6%) 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 18 (38%) 17 (35%) 17 (36%)
Artery, Inflammation, Chronic Active,
Multifocal 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50
Early Deaths
Moribund Sacrifice 9 19 17
Natural Death 4 3 18
Accidently Killed 2
Survivors
Terminal Sacrifice 37 26 15
Animals Examined Microscopically 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (40) (39) (31)
Ectasia 1 (3%)
Infiltration Cellular, Focal, Lymphocyte 2 (6%)
Infiltration Cellular, Multifocal, Lymphocyte 2 (5%) 3 (8%)
Intestine Large, Cecum (48) (47) (40)
Peyer's Patch, Hyperplasia, Lymphoid 6 (13%) 3 (6%)
Intestine Large, Colon (49) (46) (48)
Infiltration Cellular, Multifocal, Lymphocyte 1 (2%)
Intestine Small, Duodenum (49) (48) (44)
Ectopic Tissue 1 (2%)
Lumen, Hemorrhage, Acute 1 (2%)
Intestine Small, Ileum (45) (47) (44)
Peyer's Patch, Hyperplasia, Lymphoid 1 (2%)
Intestine Small, Jejunum (46) (46) (42)
Hyperplasia 1 (2%)
Lumen, Hemorrhage, Acute 1 (2%)
Liver (50) (50) (50)
Angiectasis, Multifocal 1 (2%)
Basophilic Focus 2 (4%) 2 (4%) 2 (4%)
Basophilic Focus, Multiple 1 (2%)
Cytomegaly, Multifocal 1 (2%)
Cytoplasmic Alteration, Multifocal 1 (2%)
Eosinophilic Focus 1 (2%) 1 (2%)
Fatty Change, Focal 1 (2%)
Fatty Change, Multifocal 1 (2%)
Hematopoietic Cell Proliferation, Multifocal 1 (2%) 2 (4%) 2 (4%)
Hepatodiaphragmatic Nodule 1 (2%) 1 (2%)
Hyperplasia, Focal 1 (2%)
Hyperplasia, Multifocal, Nodular 1 (2%)
Infarct 2 (4%) 5 (10%) 2 (4%)
Infiltration Cellular, Focal, Lymphocyte 1 (2%) 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 4 (8%) 1 (2%) 3 (6%)
Inflammation, Chronic, Multifocal 1 (2%) 3 (6%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Inflammation, Granulomatous, Multifocal 1 (2%)
Inflammation, Multifocal, Subacute 2 (4%) 4 (8%)
Mitotic Alteration 1 (2%)
Mixed Cell Focus 1 (2%)
Necrosis, Acute, Multifocal 3 (6%) 3 (6%) 2 (4%)
Necrosis, Chronic, Multifocal 1 (2%)
Pigmentation, Multifocal 1 (2%)
Thrombosis 1 (2%)
Artery, Mineralization, Multifocal 1 (2%)
Bile Duct, Hyperplasia, Multifocal 4 (8%) 3 (6%) 3 (6%)
Centrilobular, Fatty Change, Multifocal 2 (4%) 1 (2%)
Centrilobular, Necrosis, Acute, Multifocal 1 (2%)
Centrilobular, Necrosis, Diffuse 1 (2%) 1 (2%)
Hepatocyte, Atrophy, Multifocal 1 (2%)
Kupffer Cell, Hyperplasia, Multifocal 1 (2%)
Oval Cell, Hyperplasia 1 (2%)
Mesentery (3) (1)
Angiectasis, Multifocal 1 (33%)
Fat, Inflammation, Chronic 1 (33%)
Pancreas (50) (49) (49)
Hyperplasia, Focal 1 (2%)
Artery, Inflammation, Chronic Active 1 (2%)
Duct, Cyst 1 (2%)
Duct, Dilatation 1 (2%)
Salivary Glands (50) (48) (50)
Infiltration Cellular, Multifocal, Lymphocyte 25 (50%) 21 (44%) 7 (14%)
Stomach, Forestomach (46) (49) (50)
Inflammation, Chronic Active, Focal 1 (2%)
Ulcer 1 (2%)
Epithelium, Hyperplasia, Diffuse 1 (2%)
Epithelium, Hyperplasia, Focal 1 (2%)
Stomach, Glandular (47) (49) (49)
Inflammation, Acute, Focal 1 (2%)
Inflammation, Chronic 1 (2%)
Mucosa, Mineralization 1 (2%) 1 (2%)
Tooth (3) (2) (8)
Abscess 1 (50%)
Dysplasia 2 (67%) 2 (100%) 5 (63%)
Inflammation, Acute 1 (13%)
Inflammation, Chronic 2 (25%)
Inflammation, Chronic Active 1 (33%) 3 (38%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 11
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (1) (1)
Inflammation, Chronic 1 (100%)
Artery, Inflammation, Chronic, Multifocal 1 (100%)
Mesenteric Artery, Inflammation, Chronic 1 (100%)
Mesenteric Artery, Thrombosis 1 (100%)
Renal Artery, Inflammation, Chronic,
Multifocal 1 (100%)
Heart (49) (50) (50)
Cardiomyopathy 2 (4%)
Inflammation, Acute, Multifocal 1 (2%)
Inflammation, Chronic, Focal 1 (2%)
Inflammation, Chronic, Multifocal 1 (2%)
Aortic Valve, Inflammation, Chronic Active,
Focal 1 (2%)
Epicardium, Hyperplasia, Focal 1 (2%)
Mitral Valve, Bacterium 1 (2%)
Mitral Valve, Inflammation, Focal, Subacute 1 (2%)
Perivascular, Granuloma 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland (50) (49) (50)
Capsule, Accessory Adrenal Cortical Nodule 3 (6%)
Subcapsular, Hyperplasia, Focal 4 (8%) 4 (8%) 3 (6%)
Subcapsular, Hyperplasia, Multifocal 32 (64%) 27 (55%) 28 (56%)
Adrenal Gland, Cortex (49) (47) (50)
Cyst 1 (2%)
Hyperplasia, Focal 5 (10%) 7 (15%) 4 (8%)
Hyperplasia, Multifocal 1 (2%) 3 (6%)
Hypertrophy, Focal 6 (12%) 3 (6%) 7 (14%)
Hypertrophy, Multifocal 6 (12%) 3 (6%) 1 (2%)
Adrenal Gland, Medulla (47) (43) (47)
Hyperplasia, Focal 1 (2%) 1 (2%)
Hyperplasia, Multifocal 1 (2%)
Islets, Pancreatic (50) (49) (48)
Hyperplasia, Focal 1 (2%) 2 (4%)
Hyperplasia, Multifocal 18 (36%) 7 (14%) 23 (48%)
Pituitary Gland (48) (48) (48)
Pars Distalis, Cyst 1 (2%) 1 (2%)
Pars Distalis, Cyst, Multiple 1 (2%)
Pars Distalis, Hyperplasia, Focal 1 (2%)
Thyroid Gland (50) (48) (50)
Inflammation, Chronic, Focal 1 (2%)
Inflammation, Chronic, Multifocal 1 (2%)
Follicle, Cyst 1 (2%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 12
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Follicle, Cyst, Multiple 3 (6%) 2 (4%)
Follicular Cell, Hyperplasia, Focal 1 (2%) 2 (4%)
Follicular Cell, Hyperplasia, Multifocal 3 (6%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (50) (50) (50)
Granuloma 1 (2%)
Granuloma Sperm 1 (2%)
Inflammation, Chronic, Multifocal 1 (2%)
Penis (1) (2)
Inflammation, Chronic Active 2 (100%)
Preputial Gland (3) (4) (4)
Abscess 2 (50%)
Atrophy 2 (50%)
Ectasia 2 (67%) 1 (25%) 3 (75%)
Inflammation, Chronic 1 (33%) 1 (25%) 2 (50%)
Prostate (48) (48) (46)
Ectasia, Multifocal 1 (2%) 1 (2%)
Granuloma 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 1 (2%)
Inflammation, Acute, Suppurative 1 (2%)
Inflammation, Chronic 1 (2%)
Inflammation, Chronic Active 1 (2%) 1 (2%) 2 (4%)
Serosa, Inflammation, Acute, Focal,
Suppurative 1 (2%)
Seminal Vesicle (50) (50) (49)
Abscess 1 (2%)
Ectasia 3 (6%)
Inflammation, Chronic 1 (2%)
Testes (50) (50) (50)
Interstitial Cell, Hyperplasia, Diffuse 2 (4%)
Seminiferous Tubule, Atrophy, Multifocal 8 (16%)
Seminiferous Tubule, Degeneration, Multifocal 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 13
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (48) (50) (49)
Hyperplasia, Focal, Re Cell 1 (2%)
Metaplasia, Focal, Osseous 1 (2%)
Myeloid Cell, Hyperplasia 1 (2%) 2 (4%)
Lymph Node (50) (50) (50)
Bronchial, Hyperplasia, Lymphoid 1 (2%)
Inguinal, Hyperplasia 1 (2%)
Inguinal, Hyperplasia, Lymphoid 3 (6%)
Inguinal, Hyperplasia, Plasma Cell 1 (2%)
Mediastinal, Hyperplasia 3 (6%)
Mediastinal, Hyperplasia, Lymphoid 2 (4%) 1 (2%)
Mediastinal, Hyperplasia, Plasma Cell 1 (2%)
Lymph Node, Mandibular (37) (39) (43)
Hyperplasia 1 (3%)
Hyperplasia, Lymphoid 2 (5%) 1 (3%) 2 (5%)
Hyperplasia, Re Cell 2 (5%)
Pigmentation, Hemosiderin 1 (3%) 1 (3%) 3 (7%)
Lymph Node, Mesenteric (46) (48) (39)
Angiectasis 1 (3%)
Hematopoietic Cell Proliferation 13 (28%) 7 (15%) 5 (13%)
Hemorrhage, Acute 27 (59%) 25 (52%) 19 (49%)
Hemorrhage, Subacute 5 (11%) 3 (6%) 1 (3%)
Hyperplasia, Lymphoid 3 (7%) 4 (8%) 4 (10%)
Hyperplasia, Re Cell 1 (3%)
Pigmentation, Hemosiderin 1 (2%)
Spleen (50) (49) (50)
Angiectasis, Multifocal 1 (2%)
Fibrosis, Diffuse 1 (2%)
Hematopoietic Cell Proliferation 18 (36%) 10 (20%) 8 (16%)
Hyperplasia, Lymphoid 3 (6%) 1 (2%) 2 (4%)
Hyperplasia, Re Cell 1 (2%)
Necrosis, Focal, Subacute 1 (2%)
Thymus (45) (38) (32)
Cyst 1 (2%)
Depletion Lymphoid 2 (4%) 6 (19%)
Hyperplasia, Lymphoid 1 (2%) 1 (3%)
Necrosis 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (48) (49) (49)
Prepuce, Inflammation, Chronic Active 2 (4%) 1 (2%) 3 (6%)
Prepuce, Ulcer 1 (2%) 1 (2%) 3 (6%)
Subcutaneous Tissue, Abscess, Chronic 1 (2%)
Subcutaneous Tissue, Edema 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 14
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM - CONT
Subcutaneous Tissue, Inflammation, Diffuse,
Subacute 1 (2%)
Subcutaneous Tissue, Inflammation, Focal,
Granulomatous 1 (2%)
Subcutaneous Tissue, Inflammation, Focal,
Subacute 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (49) (50) (50)
Fibrous Osteodystrophy 3 (6%)
Osteoporosis 1 (2%)
Sternum, Developmental Malformation 2 (4%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50)
Compression 1 (2%)
Hemorrhage, Acute, Multifocal 1 (2%)
Infarct, Subacute 1 (2%)
Inflammation, Acute, Focal 1 (2%)
Mineralization, Multifocal 31 (62%) 29 (58%) 30 (60%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (50) (46) (47)
Inflammation, Chronic 1 (2%) 1 (2%)
Inflammation, Chronic Active 2 (4%)
Lung (50) (50) (50)
Hemorrhage, Acute 2 (4%) 1 (2%) 4 (8%)
Hemorrhage, Subacute 1 (2%)
Infiltration Cellular, Multifocal, Lymphocyte 4 (8%) 1 (2%) 1 (2%)
Inflammation, Acute, Focal 1 (2%)
Necrosis, Acute, Multifocal 2 (4%)
Thrombosis 1 (2%)
Alveolar Epithelium, Hyperplasia, Focal 2 (4%) 13 (26%) 1 (2%)
Alveolar Epithelium, Hyperplasia, Multifocal 8 (16%) 45 (90%)
Alveolus, Infiltration Cellular, Diffuse,
Histiocyte 1 (2%) 2 (4%)
Alveolus, Infiltration Cellular, Focal,
Histiocyte 1 (2%)
Alveolus, Infiltration Cellular, Multifocal,
Histiocyte 6 (12%) 4 (8%) 20 (40%)
Artery, Thrombosis, Multifocal 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 15
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Bronchiole, Hyperplasia, Focal 4 (8%)
Bronchiole, Hyperplasia, Multifocal 5 (10%) 40 (80%)
Bronchiole, Alveolus, Inflammation, Acute,
Multifocal, Suppurative 1 (2%)
Mediastinum, Infiltration Cellular,
Multifocal, Lymphocyte 1 (2%) 1 (2%)
Nose (49) (50) (49)
Lumen, Exudate 1 (2%) 1 (2%) 29 (59%)
Mucosa, Inflammation, Acute 1 (2%)
Mucosa, Inflammation, Chronic Active 1 (2%) 2 (4%) 5 (10%)
Nasolacrimal Duct, Exudate 1 (2%)
Nasolacrimal Duct, Hyperplasia 1 (2%)
Nasolacrimal Duct, Inflammation, Acute 1 (2%)
Nasolacrimal Duct, Inflammation, Chronic
Active 1 (2%) 1 (2%) 1 (2%)
Olfactory Epithelium, Atrophy 1 (2%)
Respiratory Epithelium, Hyperplasia 3 (6%) 6 (12%) 5 (10%)
Respiratory Epithelium, Ulcer, Focal 1 (2%)
Trachea (50) (47) (49)
Inflammation, Chronic Active 2 (4%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (1)
Atrophy 1 (100%)
Lacrimal Gland (1)
Infiltration Cellular, Multifocal, Lymphocyte 1 (100%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (50) (50)
Abscess 1 (2%)
Abscess, Multiple 1 (2%)
Hydronephrosis 1 (2%) 3 (6%) 2 (4%)
Infarct 1 (2%) 3 (6%)
Infiltration Cellular, Plasma Cell 1 (2%)
Infiltration Cellular, Lymphocyte 18 (36%) 15 (30%) 7 (14%)
Mineralization, Multifocal 2 (4%) 1 (2%)
Nephropathy, Chronic 3 (6%) 5 (10%)
Bilateral, Hydronephrosis 2 (4%)
Bilateral, Pelvis, Inflammation, Acute 2 (4%) 1 (2%)
Capsule, Fibrosis, Focal 1 (2%)
Cortex, Cyst 1 (2%)
Medulla, Necrosis, Acute, Focal 1 (2%)
Pelvis, Inflammation, Acute 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 16
NTP Experiment-Test: 05072-02 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC TETRANITROMETHANE Date: 09/09/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:17:51
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 0.5 PPM 2 PPM
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Proximal Convoluted Renal Tubule, Necrosis,
Acute, Multifocal 1 (2%)
Renal Tubule, Cytoplasmic Alteration,
Multifocal 1 (2%)
Renal Tubule, Hyperplasia, Atypical, Focal 2 (4%) 1 (2%)
Renal Tubule, Hyperplasia, Focal 1 (2%)
Renal Tubule, Hyperplasia, Multifocal 1 (2%) 2 (4%)
Urinary Bladder (48) (47) (47)
Infiltration Cellular, Multifocal, Lymphocyte 1 (2%) 1 (2%)
Inflammation, Chronic, Diffuse 1 (2%) 1 (2%)
Inflammation, Chronic, Multifocal 1 (2%)
Inflammation, Chronic Active, Diffuse 2 (4%)
Ulcer, Multifocal 1 (2%)
Transitional Epithelium, Hyperplasia,
Atypical, Diffuse 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 17
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