TR 396 Mouse Pathology Tables
NTP STATISTICAL ANALYSIS OF PRIMARY TUMORS REPORT: PEIRPT08
LAB: IRDC MONOCHLOROACETIC ACID DATE: 09/18/94
EXPERIMENT: 05705 TEST: 01 TIME: 19:59:13
TEST TYPE: CHRONIC CAGES FROM 0000 TO LAST CAGE PAGE: 1
CONT: 05700-05 ROUTE: GAVAGE NTP C#: 60231
PATHOLOGIST: D. GOODMAN S. EUSTIS CAS: 79-11-8
------------------------------------------------------------------------------------------------------------------------------------
REASONS FOR REMOVAL: ALL
REMOVAL DATE RANGE: ALL
TREATMENT GROUPS: INCLUDE ALL
NTP STATISTICAL ANALYSIS OF PRIMARY TUMORS REPORT: PEIRPT08
LAB: IRDC MONOCHLOROACETIC ACID DATE: 09/18/94
EXPERIMENT: 05705 TEST: 01 TIME: 19:59:13
TEST TYPE: CHRONIC CAGES FROM 0000 TO LAST CAGE
CONT: 05700-05 ROUTE: GAVAGE NTP C#: 60231
PATHOLOGIST: D. GOODMAN S. EUSTIS CAS: 79-11-8
MICE(B6C3F1)
----------------------------
FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.
----------------
Adrenal Gland
Bone Marrow
Brain
Clitoral Gland
Epididymis
Gallbladder
Heart
Islets, Pancreatic
Kidney
Liver
Lung
Nose
Ovary
Pancreas
Parathyroid Gland
Pituitary Gland
Preputial Gland
Prostate
Salivary Glands
Spleen
Testes
Thymus
Thyroid Gland
Urinary Bladder
NTP STATISTICAL ANALYSIS OF PRIMARY TUMORS REPORT: PEIRPT08
LAB: IRDC MONOCHLOROACETIC ACID DATE: 09/18/94
EXPERIMENT: 05705 TEST: 01 TIME: 19:59:13
TEST TYPE: CHRONIC CAGES FROM 0000 TO LAST CAGE
CONT: 05700-05 ROUTE: GAVAGE NTP C#: 60231
PATHOLOGIST: D. GOODMAN S. EUSTIS CAS: 79-11-8
SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
IN THE ANALYSIS OF THE STUDY OF MONOCHLOROACETIC ACID
====================================================================================================
MALE MICE
-------------
ORGAN MORPHOLOGY
------- ------------
Lung Alveolar/Bronchiolar Carcinoma
Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
Skin Neurofibrosarcoma
All Organs Benign Tumors
Malignant and Benign Tumors
====================================================================================================
FEMALE MICE
-------------
ORGAN MORPHOLOGY
------- ------------
Lung Alveolar/Bronchiolar Adenoma
Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
Pituitary Gland: Pars Distalis or Unspecified Site
Adenoma
Thyroid Gland: Follicular Cell
Adenoma
Carcinoma or Adenoma
All Organs Malignant Lymphoma And Histiocytic Sarcoma
Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,
NOS, or Undifferentiated Cell Type
Malignant Tumors
Malignant and Benign Tumors
====================================================================================================
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 1
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Adrenal Cortex |
| Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 2/60 (3%) 2/59 (3%) 2/60 (3%) | 1/59 (2%) 0/1 (0%) 0/60 (0%) |
|ADJUSTED (b) | 4.3% 5.1% 9.5% | 2.4% 0.0% 0.0% |
|TERMINAL (d) | 2/46 (4%) 2/39 (5%) 2/21 (10%) | 1/41 (2%) 0/0 0/44 (0%) |
|FIRST INCIDENCE (DAYS) | 729 (T) 729 (T) 729 (T) | 729 (T) --- --- |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.307 P=0.634 P=0.393 | P=0.296N (e) P=0.486N |
|INCIDENTAL TUMOR | P=0.307 P=0.634 P=0.393 | P=0.296N (e) P=0.486N |
|LOGISTIC REGRESSION | P=0.307 P=0.634 P=0.393 | P=0.296N (e) P=0.486N |
|COCHRAN-ARMITAGE | P=0.600 | P=0.304N |
|FISHER EXACT | P=0.684 P=0.691N | P=0.983N P=0.496N |
|=================================================================================================================================|
| |
|Harderian Gland |
| Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 2/60 (3%) 3/60 (5%) 2/60 (3%) | 1/60 (2%) 0/60 (0%) 0/60 (0%) |
|ADJUSTED (b) | 4.3% 7.2% 7.1% | 2.4% 0.0% 0.0% |
|TERMINAL (d) | 2/46 (4%) 2/39 (5%) 1/21 (5%) | 1/42 (2%) 0/40 (0%) 0/44 (0%) |
|FIRST INCIDENCE (DAYS) | 729 (T) 670 467 | 729 (T) --- --- |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.312 P=0.433 P=0.442 | P=0.266N P=0.510N P=0.491N |
|INCIDENTAL TUMOR | P=0.516 P=0.470 P=0.650 | P=0.266N P=0.510N P=0.491N |
|LOGISTIC REGRESSION | P=0.458 P=0.451 P=0.609 | P=0.266N P=0.510N P=0.491N |
|COCHRAN-ARMITAGE | P=0.593 | P=0.270N |
|FISHER EXACT | P=0.500 P=0.691N | P=0.500N P=0.500N |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 2
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Liver |
| Hemangioma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 2/60 (3%) 0/59 (0%) 0/59 (0%) | 0/60 (0%) 0/60 (0%) 0/60 (0%) |
|ADJUSTED (b) | 4.3% 0.0% 0.0% | 0.0% 0.0% 0.0% |
|TERMINAL (d) | 2/46 (4%) 0/39 (0%) 0/21 (0%) | 0/42 (0%) 0/40 (0%) 0/44 (0%) |
|FIRST INCIDENCE (DAYS) | 729 (T) --- --- | --- --- --- |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.168N P=0.276N P=0.423N | (e) (e) (e) |
|INCIDENTAL TUMOR | P=0.168N P=0.276N P=0.423N | (e) (e) (e) |
|LOGISTIC REGRESSION | P=0.168N P=0.276N P=0.423N | (e) (e) (e) |
|COCHRAN-ARMITAGE | P=0.098N | (e) |
|FISHER EXACT | P=0.252N P=0.252N | (e) (e) |
|=================================================================================================================================|
| |
|Liver |
| Hepatocellular Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 6/60 (10%) 6/59 (10%) 1/59 (2%) | 1/60 (2%) 1/60 (2%) 2/60 (3%) |
|ADJUSTED (b) | 12.7% 14.8% 4.2% | 2.4% 2.5% 4.5% |
|TERMINAL (d) | 5/46 (11%) 5/39 (13%) 0/21 (0%) | 1/42 (2%) 1/40 (3%) 2/44 (5%) |
|FIRST INCIDENCE (DAYS) | 702 673 695 | 729 (T) 729 (T) 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.285N P=0.505 P=0.274N | P=0.395 P=0.751 P=0.516 |
|INCIDENTAL TUMOR | P=0.189N P=0.562 P=0.169N | P=0.395 P=0.751 P=0.516 |
|LOGISTIC REGRESSION | P=0.224N P=0.535 P=0.220N | P=0.395 P=0.751 P=0.516 |
|COCHRAN-ARMITAGE | P=0.059N | P=0.378 |
|FISHER EXACT | P=0.607 P=0.060N | P=0.752N P=0.500 |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 3
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Liver |
| Hepatocellular Carcinoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 6/60 (10%) 2/59 (3%) 5/59 (8%) | 0/60 (0%) 1/60 (2%) 0/60 (0%) |
|ADJUSTED (b) | 11.7% 4.7% 19.9% | 0.0% 2.5% 0.0% |
|TERMINAL (d) | 3/46 (7%) 1/39 (3%) 3/21 (14%) | 0/42 (0%) 1/40 (3%) 0/44 (0%) |
|FIRST INCIDENCE (DAYS) | 517 670 581 | --- 729 (T) --- |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.322 P=0.188N P=0.292 | P=0.721N P=0.490 (e) |
|INCIDENTAL TUMOR | P=0.517N P=0.132N P=0.639 | P=0.721N P=0.490 (e) |
|LOGISTIC REGRESSION | P=0.530N P=0.146N P=0.617N | P=0.721N P=0.490 (e) |
|COCHRAN-ARMITAGE | P=0.440N | P=0.730 |
|FISHER EXACT | P=0.142N P=0.512N | P=0.500 (e) |
|=================================================================================================================================|
| |
|Liver |
| Hepatocellular Carcinoma or Hepatocellular Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 12/60 (20%) 8/59 (14%) 6/59 (10%) | 1/60 (2%) 2/60 (3%) 2/60 (3%) |
|ADJUSTED (b) | 23.6% 19.1% 23.3% | 2.4% 5.0% 4.5% |
|TERMINAL (d) | 8/46 (17%) 6/39 (15%) 3/21 (14%) | 1/42 (2%) 2/40 (5%) 2/44 (5%) |
|FIRST INCIDENCE (DAYS) | 517 670 581 | 729 (T) 729 (T) 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.528N P=0.359N P=0.581 | P=0.408 P=0.483 P=0.516 |
|INCIDENTAL TUMOR | P=0.224N P=0.266N P=0.259N | P=0.408 P=0.483 P=0.516 |
|LOGISTIC REGRESSION | P=0.282N P=0.295N P=0.346N | P=0.408 P=0.483 P=0.516 |
|COCHRAN-ARMITAGE | P=0.082N | P=0.391 |
|FISHER EXACT | P=0.244N P=0.107N | P=0.500 P=0.500 |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 4
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Liver |
| Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 12/60 (20%) 8/59 (14%) 6/59 (10%) | 1/60 (2%) 2/60 (3%) 2/60 (3%) |
|ADJUSTED (b) | 23.6% 19.1% 23.3% | 2.4% 5.0% 4.5% |
|TERMINAL (d) | 8/46 (17%) 6/39 (15%) 3/21 (14%) | 1/42 (2%) 2/40 (5%) 2/44 (5%) |
|FIRST INCIDENCE (DAYS) | 517 670 581 | 729 (T) 729 (T) 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.528N P=0.359N P=0.581 | P=0.408 P=0.483 P=0.516 |
|INCIDENTAL TUMOR | P=0.224N P=0.266N P=0.259N | P=0.408 P=0.483 P=0.516 |
|LOGISTIC REGRESSION | P=0.282N P=0.295N P=0.346N | P=0.408 P=0.483 P=0.516 |
|COCHRAN-ARMITAGE | P=0.082N | P=0.391 |
|FISHER EXACT | P=0.244N P=0.107N | P=0.500 P=0.500 |
|=================================================================================================================================|
| |
|Lung |
| Alveolar/Bronchiolar Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 9/60 (15%) 10/59 (17%) 5/60 (8%) | 0/60 (0%) 2/14 (14%) 2/60 (3%) |
|ADJUSTED (b) | 19.6% 25.6% 20.7% | 0.0% 21.6% 4.5% |
|TERMINAL (d) | 9/46 (20%) 10/39 (26%) 3/21 (14%) | 0/42 (0%) 1/5 (20%) 2/44 (5%) |
|FIRST INCIDENCE (DAYS) | 729 (T) 729 (T) 594 | --- 636 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.373 P=0.342 P=0.487 | P=0.217 P=0.060 P=0.249 |
|INCIDENTAL TUMOR | P=0.441 P=0.342 P=0.599 | P=0.198 P=0.079 P=0.249 |
|LOGISTIC REGRESSION | P=0.462 P=0.342 P=0.602 | P=0.192 P=0.020 * P=0.249 |
|COCHRAN-ARMITAGE | P=0.174N | P=0.211 |
|FISHER EXACT | P=0.484 P=0.197N | P=0.034 * P=0.248 |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 5
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Lung |
| Alveolar/Bronchiolar Carcinoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 4/60 (7%) 0/59 (0%) 0/60 (0%) | 0/60 (0%) 1/14 (7%) 0/60 (0%) |
|ADJUSTED (b) | 8.7% 0.0% 0.0% | 0.0% 20.0% 0.0% |
|TERMINAL (d) | 4/46 (9%) 0/39 (0%) 0/21 (0%) | 0/42 (0%) 1/5 (20%) 0/44 (0%) |
|FIRST INCIDENCE (DAYS) | 729 (T) --- --- | --- 729 (T) --- |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.044N* P=0.086N P=0.203N | P=0.689N P=0.101 (e) |
|INCIDENTAL TUMOR | P=0.044N* P=0.086N P=0.203N | P=0.689N P=0.101 (e) |
|LOGISTIC REGRESSION | P=0.043N* P=0.086N P=0.203N | P=0.689N P=0.101 (e) |
|COCHRAN-ARMITAGE | P=0.015N* | P=0.702 |
|FISHER EXACT | P=0.061N P=0.059N | P=0.189 (e) |
|=================================================================================================================================|
| |
|Lung |
| Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 12/60 (20%) 10/59 (17%) 5/60 (8%) | 0/60 (0%) 3/14 (21%) 2/60 (3%) |
|ADJUSTED (b) | 26.1% 25.6% 20.7% | 0.0% 41.2% 4.5% |
|TERMINAL (d) | 12/46 (26%) 10/39 (26%) 3/21 (14%) | 0/42 (0%) 2/5 (40%) 2/44 (5%) |
|FIRST INCIDENCE (DAYS) | 729 (T) 729 (T) 594 | --- 636 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.477N P=0.579N P=0.526N | P=0.246 P=0.003 ** P=0.249 |
|INCIDENTAL TUMOR | P=0.411N P=0.579N P=0.421N | P=0.229 P=0.004 ** P=0.249 |
|LOGISTIC REGRESSION | P=0.387N P=0.579N P=0.410N | P=0.213 P=0.001 ** P=0.249 |
|COCHRAN-ARMITAGE | P=0.049N* | P=0.235 |
|FISHER EXACT | P=0.424N P=0.057N | P=0.006 ** P=0.248 |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 6
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Mammary Gland |
| Carcinoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 0/60 (0%) 0/60 (0%) 0/60 (0%) | 2/60 (3%) 2/60 (3%) 2/60 (3%) |
|ADJUSTED (b) | 0.0% 0.0% 0.0% | 4.3% 5.0% 4.5% |
|TERMINAL (d) | 0/46 (0%) 0/39 (0%) 0/21 (0%) | 1/42 (2%) 2/40 (5%) 2/44 (5%) |
|FIRST INCIDENCE (DAYS) | --- --- --- | 682 729 (T) 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | (e) (e) (e) | P=0.588N P=0.669 P=0.686N |
|INCIDENTAL TUMOR | (e) (e) (e) | P=0.576 P=0.652 P=0.654 |
|LOGISTIC REGRESSION | (e) (e) (e) | P=0.581 P=0.668 P=0.670 |
|COCHRAN-ARMITAGE | (e) | P=0.600 |
|FISHER EXACT | (e) (e) | P=0.691N P=0.691N |
|=================================================================================================================================|
| |
|Ovary |
| Granulosa Cell Tumor, Benign, Malignant, NOS |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | | 1/59 (2%) 2/24 (8%) 0/60 (0%) |
|ADJUSTED (b) | | 2.4% 7.9% 0.0% |
|TERMINAL (d) | | 1/42 (2%) 1/17 (6%) 0/44 (0%) |
|FIRST INCIDENCE (DAYS) | | 729 (T) 651 --- |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | | P=0.369N P=0.287 P=0.491N |
|INCIDENTAL TUMOR | | P=0.417N P=0.182 P=0.491N |
|LOGISTIC REGRESSION | | P=0.389N P=0.197 P=0.491N |
|COCHRAN-ARMITAGE | | P=0.369N |
|FISHER EXACT | | P=0.199 P=0.496N |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 7
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Pituitary Gland: Pars Distalis or Unspecified Site |
| Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 0/56 (0%) 0/57 (0%) 0/56 (0%) | 5/55 (9%) 6/6 (100%) 7/57 (12%) |
|ADJUSTED (b) | 0.0% 0.0% 0.0% | 11.9% 100.0% 17.1% |
|TERMINAL (d) | 0/43 (0%) 0/38 (0%) 0/20 (0%) | 4/41 (10%) 5/5 (100%) 7/41 (17%) |
|FIRST INCIDENCE (DAYS) | --- --- --- | 724 675 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | (e) (e) (e) | P=0.342 P<0.001 ** P=0.382 |
|INCIDENTAL TUMOR | (e) (e) (e) | P=0.309 P<0.001 ** P=0.356 |
|LOGISTIC REGRESSION | (e) (e) (e) | P=0.310 P<0.001 ** P=0.375 |
|COCHRAN-ARMITAGE | (e) | P=0.377 |
|FISHER EXACT | (e) (e) | P<0.001 ** P=0.406 |
|=================================================================================================================================|
| |
|Skin |
| Fibrosarcoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 1/60 (2%) 4/60 (7%) 3/60 (5%) | 2/60 (3%) 2/60 (3%) 1/60 (2%) |
|ADJUSTED (b) | 2.2% 8.8% 9.5% | 4.6% 3.9% 2.3% |
|TERMINAL (d) | 1/46 (2%) 0/39 (0%) 0/21 (0%) | 1/42 (2%) 0/40 (0%) 1/44 (2%) |
|FIRST INCIDENCE (DAYS) | 729 (T) 581 495 | 717 545 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.080 P=0.147 P=0.127 | P=0.394N P=0.664 P=0.484N |
|INCIDENTAL TUMOR | P=0.410 P=0.236 P=0.433 | P=0.570N P=0.659 P=0.558N |
|LOGISTIC REGRESSION | P=0.341 P=0.170 P=0.267 | P=0.392N P=0.682N P=0.510N |
|COCHRAN-ARMITAGE | P=0.253 | P=0.391N |
|FISHER EXACT | P=0.182 P=0.309 | P=0.691N P=0.500N |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 8
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Skin |
| Neurofibrosarcoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 0/60 (0%) 0/60 (0%) 2/60 (3%) | 0/60 (0%) 0/60 (0%) 0/60 (0%) |
|ADJUSTED (b) | 0.0% 0.0% 8.0% | 0.0% 0.0% 0.0% |
|TERMINAL (d) | 0/46 (0%) 0/39 (0%) 1/21 (5%) | 0/42 (0%) 0/40 (0%) 0/44 (0%) |
|FIRST INCIDENCE (DAYS) | --- --- 599 | --- --- --- |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.038 * (e) P=0.104 | (e) (e) (e) |
|INCIDENTAL TUMOR | P=0.074 (e) P=0.192 | (e) (e) (e) |
|LOGISTIC REGRESSION | P=0.066 (e) P=0.170 | (e) (e) (e) |
|COCHRAN-ARMITAGE | P=0.096 | (e) |
|FISHER EXACT | (e) P=0.248 | (e) (e) |
|=================================================================================================================================|
| |
|Stomach, Forestomach |
| Squamous Cell Papilloma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 0/60 (0%) 0/60 (0%) 0/60 (0%) | 0/60 (0%) 0/60 (0%) 2/60 (3%) |
|ADJUSTED (b) | 0.0% 0.0% 0.0% | 0.0% 0.0% 4.5% |
|TERMINAL (d) | 0/46 (0%) 0/39 (0%) 0/21 (0%) | 0/42 (0%) 0/40 (0%) 2/44 (5%) |
|FIRST INCIDENCE (DAYS) | --- --- --- | --- --- 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | (e) (e) (e) | P=0.103 (e) P=0.249 |
|INCIDENTAL TUMOR | (e) (e) (e) | P=0.103 (e) P=0.249 |
|LOGISTIC REGRESSION | (e) (e) (e) | P=0.103 (e) P=0.249 |
|COCHRAN-ARMITAGE | (e) | P=0.096 |
|FISHER EXACT | (e) (e) | (e) P=0.248 |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 9
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Thyroid Gland: Follicular Cell |
| Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 1/60 (2%) 0/58 (0%) 0/57 (0%) | 1/60 (2%) 1/1 (100%) 2/60 (3%) |
|ADJUSTED (b) | 2.2% 0.0% 0.0% | 2.4% 100.0% 4.2% |
|TERMINAL (d) | 1/46 (2%) 0/39 (0%) 0/21 (0%) | 1/42 (2%) 1/1 (100%) 1/44 (2%) |
|FIRST INCIDENCE (DAYS) | 729 (T) --- --- | 729 (T) 729 (T) 640 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.364N P=0.533N P=0.656N | P=0.407 P=0.016 * P=0.506 |
|INCIDENTAL TUMOR | P=0.364N P=0.533N P=0.656N | P=0.374 P=0.016 * P=0.464 |
|LOGISTIC REGRESSION | P=0.364N P=0.533N P=0.656N | P=0.373 P=0.016 * P=0.480 |
|COCHRAN-ARMITAGE | P=0.277N | P=0.399 |
|FISHER EXACT | P=0.508N P=0.513N | P=0.033 * P=0.500 |
|=================================================================================================================================|
| |
|Thyroid Gland: Follicular Cell |
| Carcinoma or Adenoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | |
|----------- | | |
|OVERALL (a) | 2/60 (3%) 0/58 (0%) 0/57 (0%) | 1/60 (2%) 1/1 (100%) 2/60 (3%) |
|ADJUSTED (b) | 4.3% 0.0% 0.0% | 2.4% 100.0% 4.2% |
|TERMINAL (d) | 2/46 (4%) 0/39 (0%) 0/21 (0%) | 1/42 (2%) 1/1 (100%) 1/44 (2%) |
|FIRST INCIDENCE (DAYS) | 729 (T) --- --- | 729 (T) 729 (T) 640 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.168N P=0.276N P=0.423N | P=0.407 P=0.016 * P=0.506 |
|INCIDENTAL TUMOR | P=0.168N P=0.276N P=0.423N | P=0.374 P=0.016 * P=0.464 |
|LOGISTIC REGRESSION | P=0.168N P=0.276N P=0.423N | P=0.373 P=0.016 * P=0.480 |
|COCHRAN-ARMITAGE | P=0.101N | P=0.399 |
|FISHER EXACT | P=0.256N P=0.261N | P=0.033 * P=0.500 |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 10
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|Uterus |
| Carcinoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | | # # # |
|----------- | | |
|OVERALL (a) | | 0/60 (0%) 0/60 (0%) 2/60 (3%) |
|ADJUSTED (b) | | 0.0% 0.0% 4.0% |
|TERMINAL (d) | | 0/42 (0%) 0/40 (0%) 1/44 (2%) |
|FIRST INCIDENCE (DAYS) | | --- --- 100 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | | P=0.099 (e) P=0.241 |
|INCIDENTAL TUMOR | | P=0.209 (e) P=0.433 |
|LOGISTIC REGRESSION | | P=0.190 (e) P=0.391 |
|COCHRAN-ARMITAGE | | P=0.096 |
|FISHER EXACT | | (e) P=0.248 |
|=================================================================================================================================|
| |
|All Organs |
| Hemangioma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 3/60 (5%) 1/60 (2%) 0/60 (0%) | 0/60 (0%) 0/60 (0%) 1/60 (2%) |
|ADJUSTED (b) | 6.5% 2.1% 0.0% | 0.0% 0.0% 2.3% |
|TERMINAL (d) | 3/46 (7%) 0/39 (0%) 0/21 (0%) | 0/42 (0%) 0/40 (0%) 1/44 (2%) |
|FIRST INCIDENCE (DAYS) | 729 (T) 639 --- | --- --- 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.146N P=0.361N P=0.289N | P=0.279 (e) P=0.509 |
|INCIDENTAL TUMOR | P=0.104N P=0.331N P=0.289N | P=0.279 (e) P=0.509 |
|LOGISTIC REGRESSION | P=0.106N P=0.337N P=0.289N | P=0.279 (e) P=0.509 |
|COCHRAN-ARMITAGE | P=0.061N | P=0.270 |
|FISHER EXACT | P=0.309N P=0.122N | (e) P=0.500 |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 11
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|All Organs |
| Hemangiosarcoma or Hemangioma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 4/60 (7%) 2/60 (3%) 1/60 (2%) | 1/60 (2%) 0/60 (0%) 1/60 (2%) |
|ADJUSTED (b) | 8.7% 4.6% 4.8% | 1.7% 0.0% 2.3% |
|TERMINAL (d) | 4/46 (9%) 1/39 (3%) 1/21 (5%) | 0/42 (0%) 0/40 (0%) 1/44 (2%) |
|FIRST INCIDENCE (DAYS) | 729 (T) 639 729 (T) | 511 --- 729 (T) |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.326N P=0.411N P=0.473N | P=0.661 P=0.507N P=0.754 |
|INCIDENTAL TUMOR | P=0.270N P=0.385N P=0.473N | P=0.536 P=0.443N P=0.509 |
|LOGISTIC REGRESSION | P=0.265N P=0.384N P=0.473N | P=0.638N P=0.488N P=0.744N |
|COCHRAN-ARMITAGE | P=0.119N | P=0.668N |
|FISHER EXACT | P=0.340N P=0.182N | P=0.500N P=0.752N |
|=================================================================================================================================|
| |
|All Organs |
| Histiocytic Sarcoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 0/60 (0%) 0/60 (0%) 0/60 (0%) | 3/60 (5%) 2/60 (3%) 3/60 (5%) |
|ADJUSTED (b) | 0.0% 0.0% 0.0% | 6.2% 4.3% 6.3% |
|TERMINAL (d) | 0/46 (0%) 0/39 (0%) 0/21 (0%) | 1/42 (2%) 0/40 (0%) 1/44 (2%) |
|FIRST INCIDENCE (DAYS) | --- --- --- | 513 590 664 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | (e) (e) (e) | P=0.575 P=0.531N P=0.651 |
|INCIDENTAL TUMOR | (e) (e) (e) | P=0.352 P=0.475N P=0.345 |
|LOGISTIC REGRESSION | (e) (e) (e) | P=0.578N P=0.477N P=0.665 |
|COCHRAN-ARMITAGE | (e) | P=0.588N |
|FISHER EXACT | (e) (e) | P=0.500N P=0.660N |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 12
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|All Organs |
| Malignant Lymphoma And Histiocytic Sarcoma |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 8/60 (13%) 7/60 (12%) 5/60 (8%) | 32/60 (53%) 20/60 (33%) 15/60 (25%) |
|ADJUSTED (b) | 16.1% 16.0% 21.3% | 59.0% 38.0% 30.3% |
|TERMINAL (d) | 5/46 (11%) 4/39 (10%) 3/21 (14%) | 20/42 (48%) 9/40 (23%) 10/44 (23%) |
|FIRST INCIDENCE (DAYS) | 637 573 679 | 513 390 640 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.388 P=0.598 P=0.420 | P=0.003N** P=0.076N P=0.003N** |
|INCIDENTAL TUMOR | P=0.466N P=0.483N P=0.553N | P=0.010N* P=0.019N* P=0.011N* |
|LOGISTIC REGRESSION | P=0.546N P=0.560N P=0.581 | P=0.007N** P=0.021N* P=0.007N** |
|COCHRAN-ARMITAGE | P=0.234N | P<0.001N** |
|FISHER EXACT | P=0.500N P=0.279N | P=0.021N* P=0.001N** |
|=================================================================================================================================|
| |
|All Organs |
| Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed, |
| NOS, or Undifferentiated Cell Type |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 8/60 (13%) 7/60 (12%) 5/60 (8%) | 29/60 (48%) 18/60 (30%) 13/60 (22%) |
|ADJUSTED (b) | 16.1% 16.0% 21.3% | 55.4% 35.2% 27.3% |
|TERMINAL (d) | 5/46 (11%) 4/39 (10%) 3/21 (14%) | 19/42 (45%) 9/40 (23%) 10/44 (23%) |
|FIRST INCIDENCE (DAYS) | 637 573 679 | 625 390 640 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.388 P=0.598 P=0.420 | P=0.003N** P=0.085N P=0.003N** |
|INCIDENTAL TUMOR | P=0.466N P=0.483N P=0.553N | P=0.009N** P=0.033N* P=0.008N** |
|LOGISTIC REGRESSION | P=0.546N P=0.560N P=0.581 | P=0.003N** P=0.033N* P=0.004N** |
|COCHRAN-ARMITAGE | P=0.234N | P=0.001N** |
|FISHER EXACT | P=0.500N P=0.279N | P=0.030N* P=0.002N** |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 13
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|All Organs |
| Benign Tumors |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 20/60 (33%) 21/60 (35%) 11/60 (18%) | 14/60 (23%) 11/60 (18%) 13/60 (22%) |
|ADJUSTED (b) | 42.5% 49.6% 40.7% | 32.6% 25.7% 28.1% |
|TERMINAL (d) | 19/46 (41%) 18/39 (46%) 6/21 (29%) | 13/42 (31%) 9/40 (23%) 11/44 (25%) |
|FIRST INCIDENCE (DAYS) | 702 639 467 | 724 636 640 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.298 P=0.256 P=0.399 | P=0.412N P=0.381N P=0.448N |
|INCIDENTAL TUMOR | P=0.531N P=0.311 P=0.499N | P=0.478N P=0.367N P=0.520N |
|LOGISTIC REGRESSION | P=0.534 P=0.302 P=0.539N | P=0.504N P=0.424N P=0.533N |
|COCHRAN-ARMITAGE | P=0.043N* | P=0.455N |
|FISHER EXACT | P=0.500 P=0.047N* | P=0.327N P=0.500N |
|=================================================================================================================================|
| |
|All Organs |
| Malignant Tumors |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 21/60 (35%) 14/60 (23%) 13/60 (22%) | 36/60 (60%) 27/60 (45%) 20/60 (33%) |
|ADJUSTED (b) | 39.3% 29.3% 44.5% | 64.2% 47.4% 38.9% |
|TERMINAL (d) | 14/46 (30%) 6/39 (15%) 6/21 (29%) | 22/42 (52%) 11/40 (28%) 13/44 (30%) |
|FIRST INCIDENCE (DAYS) | 517 573 495 | 511 253 100 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.352 P=0.261N P=0.296 | P=0.010N* P=0.200N P=0.008N** |
|INCIDENTAL TUMOR | P=0.137N P=0.085N P=0.255N | P=0.021N* P=0.054N P=0.026N* |
|LOGISTIC REGRESSION | P=0.179N P=0.081N P=0.289N | P=0.015N* P=0.024N* P=0.006N** |
|COCHRAN-ARMITAGE | P=0.061N | P=0.002N** |
|FISHER EXACT | P=0.114N P=0.078N | P=0.072N P=0.003N** |
|=================================================================================================================================|
DATE: 09/18/94 EXPERIMENT: 05705 TEST: 01 PAGE 14
STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
| | | |
| | MALES | FEMALES |
| | | |
|DOSE | CONTROL 50 MG/KG 100MG/KG | CONTROL 50 MG/KG 100MG/KG |
| | MALE MALE MALE | FEMALE FEMALE FEMALE |
|=================================================================================================================================|
| |
|All Organs |
| Malignant and Benign Tumors |
| |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES | # # # | # # # |
|----------- | | |
|OVERALL (a) | 32/60 (53%) 32/60 (53%) 21/60 (35%) | 41/60 (68%) 33/60 (55%) 27/60 (45%) |
|ADJUSTED (b) | 60.2% 65.2% 66.6% | 73.2% 58.3% 52.7% |
|TERMINAL (d) | 25/46 (54%) 22/39 (56%) 11/21 (52%) | 27/42 (64%) 17/40 (43%) 20/44 (45%) |
|FIRST INCIDENCE (DAYS) | 517 573 467 | 511 253 100 |
|-----------------------| | |
|STATISTICAL TESTS (f) | | |
|-----------------------| | |
|LIFE TABLE | P=0.096 P=0.269 P=0.121 | P=0.021N* P=0.255N P=0.017N* |
|INCIDENTAL TUMOR | P=0.404N P=0.454 P=0.418N | P=0.052N P=0.090N P=0.058N |
|LOGISTIC REGRESSION | P=0.441N P=0.367 P=0.437N | P=0.039N* P=0.049N* P=0.021N* |
|COCHRAN-ARMITAGE | P=0.027N* | P=0.007N** |
|FISHER EXACT | P=0.573N P=0.033N* | P=0.094N P=0.008N** |
|=================================================================================================================================|
(a) Number of tumor-bearing animals / number of animals examined at site.
(b) Kaplan-Meier estimated lifetime tumor incidence after adjustment for
intercurrent mortality.
(d) Observed incidence at terminal kill.
(f) Beneath the control incidence are the P-values associated with the trend
test. Beneath the dosed group incidence are the P-values corresponding to
pairwise comparisons between the controls and that dosed group. The life
table analysis regards tumors in animals dying prior to terminal kill as
being (directly or indirectly) the cause of death. The incidental tumor
test regards these lesions as non-fatal. Logistic regression is an alternative
method for analyzing the incidence of non-fatal tumors. The Cochran-Armitage
and Fisher's exact tests compare directly the overall incidence rates.
For all tests, a negative trend is indicated by "N".
(e) Value of Statistic cannot be computed.
(I) Interim sacrifice
(T) Terminal sacrifice
# Tumor rates based on number of animals necropsied.
* To the right of any statistical result, indicates significance at (P<=0.05).
** To the right of any statistical result, indicates significance at (P<=0.01).