TDMS Study 91005-07 Pathology Tables
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
FINAL #1 RATS
Facility: Southern Research Institute
Chemical CAS #: 7220-79-3
Lock Date: 09/05/03
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
Page 1
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|
| 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM | |
|__________________________________________________________________________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Jejunum | + + + + + + + + + + + A + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +|
Basophilic Focus | X X X X X X 2 X X X X 2 X X X X X|
Clear Cell Focus | X X |
Degeneration, Cystic | 1 |
Eosinophilic Focus | X |
Hepatodiaphragmatic Nodule | X 2 3 |
Inflammation, Chronic | 1 2 2 |
Necrosis, Focal | |
Bile Duct, Hyperplasia | 2 2 2 |
Centrilobular, Necrosis | |
Hepatocyte, Vacuolization Cytoplasmic| 2 2 3 2 |
|__________________________________________________________________________|
Mesentery | + + + + + + + + + + +|
Fat, Necrosis | 3 4 2 3 3 3 2 3 2 3 3|
|__________________________________________________________________________|
Oral Mucosa | + |
Pharyngeal, Cyst | |
|__________________________________________________________________________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +|
Atrophy | 2 2 1 1 1 3 1 1 1 2 1 2 1 1 2 1 |
Acinus, Hyperplasia, Focal | 2 |
|__________________________________________________________________________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +|
__________________________________________|__________________________________________________________________________|
Page 2
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|
| 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM - cont | |
|__________________________________________________________________________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 |
Inflammation, Chronic Active | |
Ulcer | 3 2 |
Epithelium, Hyperplasia | |
|__________________________________________________________________________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 |
Erosion | 2 3 |
Ulcer | 2 |
|__________________________________________________________________________|
Tongue | + |
Hyperplasia | 2 |
_____________________________________________________________________________________________________________________|
CARDIOVASCULAR SYSTEM | |
|__________________________________________________________________________|
Blood Vessel | + |
|__________________________________________________________________________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cardiomyopathy | 1 1 1 1 1 1 2 1 1 1 1 1 2 2 1 2 1 |
_____________________________________________________________________________________________________________________|
ENDOCRINE SYSTEM | |
|__________________________________________________________________________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +|
Accessory Adrenal Cortical Nodule | 1 1 1 1 1 |
Degeneration, Fatty | 1 1 3 1 2 1 2 1 |
Hyperplasia, Focal | 1 2 2 |
Hypertrophy, Focal | 2 1 3 2 2 |
Necrosis | 2 |
|__________________________________________________________________________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | |
|__________________________________________________________________________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 1 1 1 1 1 1 1 1 |
Metaplasia, Hepatocyte | 2 |
|__________________________________________________________________________|
__________________________________________|__________________________________________________________________________|
Page 3
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|
| 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ENDOCRINE SYSTEM - cont | |
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + M + + + +|
|__________________________________________________________________________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Pars Distalis, Angiectasis | 2 2 2 2 2 2 2 2 2 2 2 2 |
Pars Distalis, Cyst | 2 2 2 2 2 2 3 3 2 2 2|
Pars Distalis, Hyperplasia, Focal | 2 |
Pars Intermedia, Cyst | |
|__________________________________________________________________________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ultimobranchial Cyst | 1 |
C-Cell, Hyperplasia | 2 3 2 2 1|
Follicle, Cyst | 2 2 |
_____________________________________________________________________________________________________________________|
GENERAL BODY SYSTEM | |
None | |
_____________________________________________________________________________________________________________________|
GENITAL SYSTEM | |
|__________________________________________________________________________|
Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 3 |
Inflammation, Chronic | |
|__________________________________________________________________________|
Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 1 2 3 1 |
|__________________________________________________________________________|
Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | X |
Hemorrhage | |
Hydrometra | 3 |
Cervix, Cyst | X |
Endometrium, Hyperplasia, Cystic | 2 |
_____________________________________________________________________________________________________________________|
HEMATOPOIETIC SYSTEM | |
|__________________________________________________________________________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 4 2 1 2|
|__________________________________________________________________________|
Lymph Node | + + + + + + |
__________________________________________|__________________________________________________________________________|
Page 4
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|
| 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
HEMATOPOIETIC SYSTEM - cont | |
Mediastinal, Congestion | |
Mediastinal, Hyperplasia, Lymphoid | |
Pancreatic, Hyperplasia, Lymphoid | |
|__________________________________________________________________________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M|
|__________________________________________________________________________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +|
Fibrosis | |
Hematopoietic Cell Proliferation | 4 |
Necrosis | 3 |
Capsule, Fibrosis | 1 1 1 1|
Lymphoid Follicle, Hyperplasia | |
|__________________________________________________________________________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
INTEGUMENTARY SYSTEM | |
|__________________________________________________________________________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia, Atypical | 1 |
Hyperplasia | 2 4 3 2 2|
Duct, Dilatation | 3 2 2 2 2 3 3 2 4 2 2 2 3 3 4 2 3 2 |
Duct, Hyperplasia | 2 2 |
|__________________________________________________________________________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
MUSCULOSKELETAL SYSTEM | |
|__________________________________________________________________________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cranium, Osteopetrosis | 2 |
|__________________________________________________________________________|
Skeletal Muscle | |
_____________________________________________________________________________________________________________________|
NERVOUS SYSTEM | |
|__________________________________________________________________________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +|
Compression | 2 |
__________________________________________|__________________________________________________________________________|
Page 5
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 5| 7| 6| 7| 7| 6| 7| 6| 6| 7| 6| 6| 7| 3| 7| 6| 7| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 3| 3| 9| 3| 4| 3| 3| 5| 3| 5| 7| 3| 4| 3| 3| 4| 3| 9| 3| 3| 3| 3| 3| 1|
| 4| 4| 4| 1| 4| 8| 6| 6| 2| 6| 2| 4| 4| 0| 8| 4| 4| 4| 8| 4| 6| 6| 6| 6| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
NERVOUS SYSTEM - cont | |
Hemorrhage | 2 |
|__________________________________________________________________________|
Peripheral Nerve | + |
|__________________________________________________________________________|
Spinal Cord | + |
_____________________________________________________________________________________________________________________|
RESPIRATORY SYSTEM | |
|__________________________________________________________________________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +|
Congestion | 2 |
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 1 1 1|
Alveolar Epithelium, Hyperplasia | |
|__________________________________________________________________________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | 2 |
Inflammation, Chronic | 2 2 2 2 |
|__________________________________________________________________________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
SPECIAL SENSES SYSTEM | |
|__________________________________________________________________________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cataract | 2 |
Cornea, Inflammation, Chronic | |
Retina, Degeneration | 3 |
|__________________________________________________________________________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 1 |
Pigmentation | 2 2 |
_____________________________________________________________________________________________________________________|
URINARY SYSTEM | |
|__________________________________________________________________________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +|
Infarct | 2 |
Nephropathy | 1 1 1 1 1 1 |
|__________________________________________________________________________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +|
__________________________________________|__________________________________________________________________________
Page 6
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3| |
| 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Basophilic Focus | 2 X X X X X X X X X X X X X X X X X X X| 37 2.0|
Clear Cell Focus | X X X X X X | 8 |
Degeneration, Cystic | | 1 1.0|
Eosinophilic Focus | 2 X | 3 2.0|
Hepatodiaphragmatic Nodule | 2 2 2 X | 7 2.2|
Inflammation, Chronic | 1 4 2 | 6 2.0|
Necrosis, Focal | 2 | 1 2.0|
Bile Duct, Hyperplasia | 2 4 2 3 | 7 2.4|
Centrilobular, Necrosis | 2 1 | 2 1.5|
Hepatocyte, Vacuolization Cytoplasmic| | 4 2.3|
|__________________________________________________________________________|____________|
Mesentery | + + + + + +| 17 |
Fat, Necrosis | 2 3 3 3 3 3| 17 2.8|
|__________________________________________________________________________|____________|
Oral Mucosa | + | 2 |
Pharyngeal, Cyst | 3 | 1 3.0|
|__________________________________________________________________________|____________|
Pancreas | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 7
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3| |
| 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM - cont | | |
Atrophy | 1 1 2 1 4 1 2 1 1 1 | 26 1.5|
Acinus, Hyperplasia, Focal | 2 1 | 3 1.7|
|__________________________________________________________________________|____________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Edema | 3 | 2 2.5|
Inflammation, Chronic Active | 1 | 1 1.0|
Ulcer | 2 2 | 4 2.3|
Epithelium, Hyperplasia | 2 2 | 2 2.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Edema | 2 | 2 2.0|
Erosion | | 2 2.5|
Ulcer | 2 | 2 2.0|
|__________________________________________________________________________|____________|
Tongue | | 1 |
Hyperplasia | | 1 2.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Blood Vessel | | 1 |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cardiomyopathy | 2 1 2 1 1 1 2 1 1 2 2 2 1 1 1 1 1 2 1 | 36 1.3|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Accessory Adrenal Cortical Nodule | 4 1 1 1 | 9 1.3|
Degeneration, Fatty | 1 1 1 | 11 1.4|
Hyperplasia, Focal | 1 1 | 5 1.4|
Hypertrophy, Focal | 2 1 2 2 1 4 1 | 12 1.9|
Necrosis | 4 | 2 3.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 8
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3| |
| 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Islets, Pancreatic | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 |
Hyperplasia | 1 1 1 1 1 | 13 1.0|
Metaplasia, Hepatocyte | 1 | 2 1.5|
|__________________________________________________________________________|____________|
Parathyroid Gland | + + + + + M + + + + + + + M + + + + + + + + + + +| 47 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Pars Distalis, Angiectasis | 2 2 3 3 2 2 1 2 2 2 2 2 2 2 2 2| 28 2.0|
Pars Distalis, Cyst | 2 2 2 4 2 2 2 2 2 1 | 21 2.1|
Pars Distalis, Hyperplasia, Focal | 2 2 2 2 | 5 2.0|
Pars Intermedia, Cyst | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ultimobranchial Cyst | 2 | 2 1.5|
C-Cell, Hyperplasia | 2 2 2 2 3 | 10 2.1|
Follicle, Cyst | | 2 2.0|
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 2 | 4 2.3|
Inflammation, Chronic | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 1 1 4 2 2| 11 1.9|
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 9
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3| |
| 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
GENITAL SYSTEM - cont | | |
Cyst | | 1 |
Hemorrhage | 2 | 1 2.0|
Hydrometra | | 1 3.0|
Cervix, Cyst | | 1 |
Endometrium, Hyperplasia, Cystic | 3 2 3 | 4 2.5|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 3 2 3 | 8 2.4|
|__________________________________________________________________________|____________|
Lymph Node | + + + + + + + + +| 15 |
Mediastinal, Congestion | 3 | 1 3.0|
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 2| 5 2.0|
Pancreatic, Hyperplasia, Lymphoid | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M| |
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Spleen | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 |
Fibrosis | 2 2 | 2 2.0|
Hematopoietic Cell Proliferation | 4 4 | 3 4.0|
Necrosis | | 1 3.0|
Capsule, Fibrosis | 1 1 1 1 | 8 1.0|
Lymphoid Follicle, Hyperplasia | 3 | 1 3.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia, Atypical | 1 2 | 3 1.3|
Hyperplasia | 2 2 3 2 2 2 3 2 2 2 2 2 2 | 18 2.3|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 10
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3| |
| 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
INTEGUMENTARY SYSTEM - cont | | |
Duct, Dilatation | 3 2 2 2 2 2 4 4 2 4 2 2 3| 31 2.6|
Duct, Hyperplasia | | 2 2.0|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cranium, Osteopetrosis | | 1 2.0|
|__________________________________________________________________________|____________|
Skeletal Muscle | + | 1 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Compression | 3 2 | 3 2.3|
Hemorrhage | | 1 2.0|
|__________________________________________________________________________|____________|
Peripheral Nerve | + + | 3 |
|__________________________________________________________________________|____________|
Spinal Cord | + + | 3 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Congestion | 1 1 1 1 | 5 1.2|
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 41 1.0|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 29 1.0|
Alveolar Epithelium, Hyperplasia | 3 | 1 3.0|
|__________________________________________________________________________|____________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Foreign Body | 1 2 2 | 4 1.8|
Inflammation, Chronic | 1 2 2 2 | 8 1.9|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 11
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 7| 7| 6| 5| 7| 7| 7| 7| 7| 6| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 1| 3| 3| 3| 3| 3| 3| 5| 6| 3| 3| 3| 3| 3| 6| 3| 4| 3| 3| 3| 3| 3| 3| |
| 4| 4| 6| 4| 4| 7| 7| 7| 7| 2| 3| 7| 7| 7| 7| 4| 8| 4| 8| 4| 3| 3| 3| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
RESPIRATORY SYSTEM - cont | | |
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cataract | | 1 2.0|
Cornea, Inflammation, Chronic | 2 | 1 2.0|
Retina, Degeneration | 2 | 2 2.5|
|__________________________________________________________________________|____________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 2 2 | 3 1.7|
Pigmentation | | 2 2.0|
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Infarct | | 1 2.0|
Nephropathy | 1 1 1 1 1 1 1 1 | 14 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 12
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|
| 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM | |
|__________________________________________________________________________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Colon | + + A + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Cecum | + + A + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Duodenum | + + A + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Jejunum | + + A + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Ileum | + + A + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +|
Basophilic Focus | X X 1 X 1 X X X X X X X X X X X X |
Clear Cell Focus | X |
Degeneration, Cystic | |
Eosinophilic Focus | X |
Hepatodiaphragmatic Nodule | X 2 |
Inflammation, Chronic | 2 2 1 |
Bile Duct, Hyperplasia | 1 2 |
Hepatocyte, Vacuolization Cytoplasmic| 1 2 2 2 |
|__________________________________________________________________________|
Mesentery | + + + + + |
Accessory Spleen | |
Fat, Necrosis | 2 3 3 3 2 |
|__________________________________________________________________________|
Pancreas | + + A + + + + + + + + + + + + + + + + + + + + + +|
Atrophy | 2 2 1 1 1 1 2 1 1 |
Inflammation | 1 |
Acinus, Hyperplasia, Focal | 2 |
|__________________________________________________________________________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 3 |
__________________________________________|__________________________________________________________________________|
Page 13
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|
| 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM - cont | |
Ulcer | 3 4 |
Epithelium, Hyperplasia | 2 |
|__________________________________________________________________________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 |
Erosion | 3 |
Ulcer | 3 |
|__________________________________________________________________________|
Tongue | |
Cyst | |
Inflammation | |
_____________________________________________________________________________________________________________________|
CARDIOVASCULAR SYSTEM | |
|__________________________________________________________________________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cardiomyopathy | 1 1 1 1 1 1 1 1 1 1 1 1 2 1 2 1 2 1 2 2 2|
Thrombosis | 2 |
_____________________________________________________________________________________________________________________|
ENDOCRINE SYSTEM | |
|__________________________________________________________________________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +|
Accessory Adrenal Cortical Nodule | 1 2 1 2|
Degeneration, Fatty | 2 2 2 1 2 2 3|
Hyperplasia, Focal | 1 2 1 |
Hypertrophy, Focal | 2 2 3 2 2 2 3|
Necrosis | |
|__________________________________________________________________________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 1 1|
|__________________________________________________________________________|
Islets, Pancreatic | + + A + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 1 1 1 1 1 |
|__________________________________________________________________________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Pars Distalis, Angiectasis | 2 1 2 2 2 2 2 1 2 2 2 2 1|
Pars Distalis, Cyst | 2 2 1 1 2 3 2 2 2 1 1 1 |
__________________________________________|__________________________________________________________________________|
Page 14
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|
| 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ENDOCRINE SYSTEM - cont | |
Pars Distalis, Hyperplasia, Focal | 1 2 2 2 2 |
|__________________________________________________________________________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
C-Cell, Hyperplasia | 1 1 1 2 2 2 |
Follicle, Cyst | |
_____________________________________________________________________________________________________________________|
GENERAL BODY SYSTEM | |
None | |
_____________________________________________________________________________________________________________________|
GENITAL SYSTEM | |
|__________________________________________________________________________|
Clitoral Gland | + + M + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 3 2 2 2 |
Inflammation, Chronic | 2 1 2 2 |
|__________________________________________________________________________|
Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 1 1 3 |
|__________________________________________________________________________|
Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | X X |
Hydrometra | 1 1 1 |
Endometrium, Hyperplasia, Cystic | 1 |
_____________________________________________________________________________________________________________________|
HEMATOPOIETIC SYSTEM | |
|__________________________________________________________________________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 4 3 2 2 2 3 |
|__________________________________________________________________________|
Lymph Node | + + + + + |
Mediastinal, Hyperplasia, Lymphoid | 2 2 3 2 2 |
|__________________________________________________________________________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M|
|__________________________________________________________________________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia, Lymphoid | 2 |
|__________________________________________________________________________|
Spleen | + + A + + + + + + + + + + + + + + + + + + + + + +|
Hematopoietic Cell Proliferation | 3 2 3 |
__________________________________________|__________________________________________________________________________|
Page 15
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|
| 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
HEMATOPOIETIC SYSTEM - cont | |
Capsule, Fibrosis | 1 1 1 1 1 1 1 1 1 1 |
|__________________________________________________________________________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
INTEGUMENTARY SYSTEM | |
|__________________________________________________________________________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Galactocele | X |
Hyperplasia, Atypical | 2 2 |
Hyperplasia | 2 2 2 2 2 2 3 2|
Duct, Dilatation | 2 2 2 3 2 3 2 2 2 2 2 4 4 2 3 2 2 |
|__________________________________________________________________________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 1 |
Inflammation, Chronic Active | 4 |
_____________________________________________________________________________________________________________________|
MUSCULOSKELETAL SYSTEM | |
|__________________________________________________________________________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
NERVOUS SYSTEM | |
|__________________________________________________________________________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +|
Compression | 3 2 2 |
Hemorrhage | |
|__________________________________________________________________________|
Peripheral Nerve | + + |
|__________________________________________________________________________|
Spinal Cord | + + |
_____________________________________________________________________________________________________________________|
RESPIRATORY SYSTEM | |
|__________________________________________________________________________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +|
Congestion | 1 |
Infiltration Cellular, Histiocyte | 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1|
Alveolar Epithelium, Hyperplasia | |
|__________________________________________________________________________|
__________________________________________|__________________________________________________________________________|
Page 16
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 6| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 3| 7| 7| 6| 7| 7| 7| 7| 7|
DAY ON TEST | 3| 8| 4| 0| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 2| 3| 9| 0| 3| 4| 3| 3| 3| 3| 3|
| 6| 7| 4| 2| 6| 7| 7| 7| 7| 7| 6| 6| 6| 6| 8| 7| 5| 2| 7| 1| 4| 4| 4| 4| 4|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
RESPIRATORY SYSTEM - cont | |
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | 1 |
Inflammation, Chronic | 1 1 1 |
|__________________________________________________________________________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
SPECIAL SENSES SYSTEM | |
|__________________________________________________________________________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cataract | 3 |
Inflammation, Chronic | 4 |
Retina, Degeneration | 4 |
|__________________________________________________________________________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 1 1 |
Pigmentation | |
|__________________________________________________________________________|
Zymbal's Gland | + |
_____________________________________________________________________________________________________________________|
URINARY SYSTEM | |
|__________________________________________________________________________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +|
Nephropathy | 1 1 1 1 2 1 1 1 1 1|
|__________________________________________________________________________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +|
__________________________________________|__________________________________________________________________________
Page 17
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7| |
DAY ON TEST | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3| |
| 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Basophilic Focus | X X X X X X X X X X X X X X X X X| 34 1.0|
Clear Cell Focus | X X X X | 5 |
Degeneration, Cystic | 2 | 1 2.0|
Eosinophilic Focus | X| 2 |
Hepatodiaphragmatic Nodule | 2 2 | 4 2.0|
Inflammation, Chronic | 2 | 4 1.8|
Bile Duct, Hyperplasia | 2 2 2 | 5 1.8|
Hepatocyte, Vacuolization Cytoplasmic| | 4 1.8|
|__________________________________________________________________________|____________|
Mesentery | + + + + + + + + + | 14 |
Accessory Spleen | 2 | 1 2.0|
Fat, Necrosis | 2 3 3 2 2 3 3 3 3 | 14 2.6|
|__________________________________________________________________________|____________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
Atrophy | 1 2 2 1 2 1 1 1 1 1 2 | 20 1.4|
Inflammation | | 1 1.0|
Acinus, Hyperplasia, Focal | | 1 2.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 18
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7| |
DAY ON TEST | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3| |
| 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM - cont | | |
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Edema | 2 | 3 2.3|
Ulcer | 2 | 3 3.0|
Epithelium, Hyperplasia | 2 | 2 2.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Edema | | 1 2.0|
Erosion | 2 | 2 2.5|
Ulcer | | 1 3.0|
|__________________________________________________________________________|____________|
Tongue | + | 1 |
Cyst | 2 | 1 2.0|
Inflammation | 3 | 1 3.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Cardiomyopathy | 1 1 1 1 1 2 1 1 1 1 1 1 1 2 1 1 2 1 1 1| 41 1.2|
Thrombosis | | 1 2.0|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Accessory Adrenal Cortical Nodule | 1 1 1 | 7 1.3|
Degeneration, Fatty | 2 2 2 2 1 1 | 13 1.8|
Hyperplasia, Focal | 2 2 2 1 2| 8 1.6|
Hypertrophy, Focal | 2 2 2 1 | 11 2.1|
Necrosis | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Hyperplasia | | 2 1.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 19
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7| |
DAY ON TEST | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3| |
| 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
Hyperplasia | 1 1 1 1 1 1 1 1 1 1 | 15 1.0|
|__________________________________________________________________________|____________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Pars Distalis, Angiectasis | 2 2 2 2 2 2 2 2 2 2 2 2 2 2| 27 1.9|
Pars Distalis, Cyst | 1 2 2 2 2 1 1 | 19 1.6|
Pars Distalis, Hyperplasia, Focal | 1 2 2 | 8 1.8|
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
C-Cell, Hyperplasia | 1 2 1 1 1 | 11 1.4|
Follicle, Cyst | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
Cyst | 3 1 1 | 7 2.0|
Inflammation, Chronic | 1 | 5 1.6|
|__________________________________________________________________________|____________|
Ovary | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Cyst | 2 | 4 1.8|
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Cyst | X | 3 |
Hydrometra | 1| 4 1.0|
Endometrium, Hyperplasia, Cystic | | 1 1.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 20
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7| |
DAY ON TEST | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3| |
| 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM - cont | | |
Hyperplasia | 2 4 | 8 2.8|
|__________________________________________________________________________|____________|
Lymph Node | + + + | 8 |
Mediastinal, Hyperplasia, Lymphoid | 2 2 | 7 2.1|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M| |
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Hyperplasia, Lymphoid | | 1 2.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
Hematopoietic Cell Proliferation | 2 2 | 5 2.4|
Capsule, Fibrosis | 1 2 2 1 1 1 1 | 17 1.1|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Galactocele | | 1 |
Hyperplasia, Atypical | 2 | 3 2.0|
Hyperplasia | 3 1 2 2 2 1 3 2 2 2 2| 19 2.1|
Duct, Dilatation | 2 3 3 2 4 3 2 3 2 2 4 | 28 2.5|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Inflammation, Chronic | 2 | 2 1.5|
Inflammation, Chronic Active | | 1 4.0|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 21
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7| |
DAY ON TEST | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3| |
| 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
NERVOUS SYSTEM - cont | | |
Brain | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Compression | 2 2 2 2 2| 8 2.1|
Hemorrhage | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Peripheral Nerve | + + | 4 |
|__________________________________________________________________________|____________|
Spinal Cord | + | 3 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Congestion | 1 | 2 1.0|
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 37 1.0|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 33 1.0|
Alveolar Epithelium, Hyperplasia | 2 2 2 2 | 4 2.0|
|__________________________________________________________________________|____________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Foreign Body | 1 1 | 3 1.0|
Inflammation, Chronic | 1 1 1 1 | 7 1.0|
|__________________________________________________________________________|____________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Cataract | 3 | 2 3.0|
Inflammation, Chronic | 4 | 2 4.0|
Retina, Degeneration | 4 | 2 4.0|
|__________________________________________________________________________|____________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Inflammation, Chronic | 2 | 3 1.3|
Pigmentation | 2 2 | 2 2.0|
|__________________________________________________________________________|____________|
Zymbal's Gland | | 1 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 22
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 5| 7| 6| 7| 5| 7| 7| 6| 7| 7| 7| 5| 6| 7| 7| 7| 7| 6| 7| 1| 7| 7| 7| 7| |
DAY ON TEST | 3| 6| 3| 4| 3| 7| 3| 3| 9| 3| 3| 3| 8| 2| 0| 3| 3| 3| 6| 3| 3| 1| 3| 3| 3| |
| 3| 8| 3| 8| 3| 6| 4| 4| 4| 4| 4| 4| 5| 1| 2| 3| 3| 3| 3| 3| 1| 0| 6| 6| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Nephropathy | 1 1 1 1 2| 15 1.1|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 23
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|
DAY ON TEST | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|
| 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM | |
|__________________________________________________________________________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +|
Perforation | |
|__________________________________________________________________________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + A + + +|
|__________________________________________________________________________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Jejunum | + + + + + A + + + + + + + M + + + + + + + A + + +|
|__________________________________________________________________________|
Intestine Small, Ileum | + + + A + A + + + + + + + A + + + + + + + + + + +|
|__________________________________________________________________________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +|
Basophilic Focus | X X X X X X X X X X X X X X X X X X |
Clear Cell Focus | X X X X |
Hepatodiaphragmatic Nodule | 2 2 2 X |
Inflammation, Chronic | 2 |
Necrosis, Focal | 1|
Bile Duct, Hyperplasia | 1 2 |
Centrilobular, Necrosis | 2 |
|__________________________________________________________________________|
Mesentery | + + + + |
Fat, Necrosis | 2 3 3 2 |
|__________________________________________________________________________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +|
Atrophy | 1 1 1 1 2 1 2 1 1 1 1 1 1|
Acinus, Hyperplasia, Focal | 2 |
|__________________________________________________________________________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation | |
|__________________________________________________________________________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 3 |
Ulcer | 3 |
__________________________________________|__________________________________________________________________________|
Page 24
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|
DAY ON TEST | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|
| 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM - cont | |
Epithelium, Hyperplasia | 1 |
|__________________________________________________________________________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 |
Ulcer | 2 |
|__________________________________________________________________________|
Tongue | + |
_____________________________________________________________________________________________________________________|
CARDIOVASCULAR SYSTEM | |
|__________________________________________________________________________|
Blood Vessel | + |
|__________________________________________________________________________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cardiomyopathy | 1 1 2 1 1 1 1 1 1 1 2 1 1 1 2 1 1 1 1|
_____________________________________________________________________________________________________________________|
ENDOCRINE SYSTEM | |
|__________________________________________________________________________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +|
Accessory Adrenal Cortical Nodule | 1 2 1 |
Degeneration, Fatty | 2 2 1 2 2 2 2 2 |
Hyperplasia, Focal | 1 1 1 1 2 |
Hypertrophy, Focal | 2 2 1 2 2 2 2 2 |
|__________________________________________________________________________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 3 |
|__________________________________________________________________________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 1 1 1 1 1 1 |
|__________________________________________________________________________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Pars Distalis, Angiectasis | 3 2 2 2 2 2 2 2 2 2 2 2 2 2|
Pars Distalis, Cyst | 2 2 2 2 2 1 2 1 2|
Pars Distalis, Hyperplasia, Focal | 2 2 |
|__________________________________________________________________________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ultimobranchial Cyst | 1 |
__________________________________________|__________________________________________________________________________|
Page 25
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|
DAY ON TEST | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|
| 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ENDOCRINE SYSTEM - cont | |
C-Cell, Hyperplasia | 3 1 3 1 1 1 |
Follicle, Cyst | 1 |
_____________________________________________________________________________________________________________________|
GENERAL BODY SYSTEM | |
None | |
_____________________________________________________________________________________________________________________|
GENITAL SYSTEM | |
|__________________________________________________________________________|
Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 2 |
Inflammation, Chronic | 4 |
|__________________________________________________________________________|
Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 3 2 1 1 |
|__________________________________________________________________________|
Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | X |
Hydrometra | 1 2|
|__________________________________________________________________________|
Vagina | |
Cyst | |
_____________________________________________________________________________________________________________________|
HEMATOPOIETIC SYSTEM | |
|__________________________________________________________________________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 3 2 3 2 |
|__________________________________________________________________________|
Lymph Node | + + + + + +|
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 |
Pancreatic, Hyperplasia, Lymphoid | |
|__________________________________________________________________________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M|
|__________________________________________________________________________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia, Lymphoid | 1 |
|__________________________________________________________________________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hematopoietic Cell Proliferation | 2 4 2 1 |
__________________________________________|__________________________________________________________________________|
Page 26
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|
DAY ON TEST | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|
| 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
HEMATOPOIETIC SYSTEM - cont | |
Capsule, Fibrosis | 1 1 2 1 1 1 1 1 |
Lymphoid Follicle, Atrophy | 3 |
|__________________________________________________________________________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
INTEGUMENTARY SYSTEM | |
|__________________________________________________________________________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | X|
Galactocele | X |
Hyperplasia, Atypical | |
Hyperplasia | 2 2 3 2|
Duct, Dilatation | 2 2 1 2 1 2 2 4 2 2 2 2 2 2 4 3 1 |
|__________________________________________________________________________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
MUSCULOSKELETAL SYSTEM | |
|__________________________________________________________________________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation | |
_____________________________________________________________________________________________________________________|
NERVOUS SYSTEM | |
|__________________________________________________________________________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +|
Compression | 3 2 2 2 2 |
Hemorrhage | |
|__________________________________________________________________________|
Peripheral Nerve | + + |
|__________________________________________________________________________|
Spinal Cord | + |
_____________________________________________________________________________________________________________________|
RESPIRATORY SYSTEM | |
|__________________________________________________________________________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +|
Congestion | 1 1 |
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 1 1|
Inflammation, Suppurative | 3 |
__________________________________________|__________________________________________________________________________|
Page 27
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 7| 7| 7| 7| 5| 5| 7| 5| 7| 4| 7|
DAY ON TEST | 3| 0| 3| 3| 3| 5| 3| 3| 3| 3| 4| 3| 3| 2| 3| 3| 3| 3| 9| 6| 3| 9| 3| 9| 3|
| 3| 2| 3| 0| 3| 1| 6| 6| 6| 6| 6| 6| 6| 1| 6| 6| 6| 6| 6| 3| 6| 3| 6| 2| 6|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
RESPIRATORY SYSTEM - cont | |
Necrosis | 3 |
Alveolar Epithelium, Hyperplasia | 2 |
|__________________________________________________________________________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | |
Inflammation, Chronic | 2 2 3 2 2 |
|__________________________________________________________________________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
SPECIAL SENSES SYSTEM | |
|__________________________________________________________________________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cataract | 1 1|
Anterior Chamber, Inflammation | |
Cornea, Inflammation, Chronic | 2 |
Retina, Degeneration | 3|
|__________________________________________________________________________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 1 |
Pigmentation | 2 |
|__________________________________________________________________________|
Zymbal's Gland | |
_____________________________________________________________________________________________________________________|
URINARY SYSTEM | |
|__________________________________________________________________________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | |
Infarct | 1 2 |
Nephropathy | 1 1 1 1 1 2 1 1 2 1 1 1|
|__________________________________________________________________________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +|
__________________________________________|__________________________________________________________________________
Page 28
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3| |
| 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Perforation | 3 | 1 3.0|
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + A + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + A + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + A + A + + + + + + + + + +| 47 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + A + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + A + A + + + + + + + + + +| 45 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + A + + + + + + + + + +| 46 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Basophilic Focus | 2 X X X X X X X X X 1 X X X X X X 2 X X| 38 1.7|
Clear Cell Focus | X X X X X | 9 |
Hepatodiaphragmatic Nodule | 2 2 X | 7 2.0|
Inflammation, Chronic | 2 1 2 | 4 1.8|
Necrosis, Focal | | 1 1.0|
Bile Duct, Hyperplasia | 1 2 2 2 | 6 1.7|
Centrilobular, Necrosis | | 1 2.0|
|__________________________________________________________________________|____________|
Mesentery | + + + + + + + + | 12 |
Fat, Necrosis | 3 2 4 3 3 2 2 | 11 2.6|
|__________________________________________________________________________|____________|
Pancreas | + + + + + + + + + + + + A + A + + + + + + + + + +| 48 |
Atrophy | 2 2 1 1 1 2 1 1 2 1 | 23 1.3|
Acinus, Hyperplasia, Focal | 2 | 2 2.0|
|__________________________________________________________________________|____________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation | 1 | 1 1.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 29
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3| |
| 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + A + + + + + + + + + +| 49 |
Edema | | 1 3.0|
Ulcer | | 1 3.0|
Epithelium, Hyperplasia | 2 | 2 1.5|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + A + A + + + + + + + + + +| 48 |
Edema | | 1 2.0|
Ulcer | | 1 2.0|
|__________________________________________________________________________|____________|
Tongue | | 1 |
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Blood Vessel | | 1 |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cardiomyopathy | 2 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 2| 36 1.2|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Accessory Adrenal Cortical Nodule | 1 1 1 2 1 | 8 1.3|
Degeneration, Fatty | 2 2 3 1 2 | 13 1.9|
Hyperplasia, Focal | 1 2 2 | 8 1.4|
Hypertrophy, Focal | 3 3 4 3 1 | 13 2.2|
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | | 1 3.0|
|__________________________________________________________________________|____________|
Islets, Pancreatic | + + + + + + + + + + + + A + A + + + + + + + + + +| 48 |
Hyperplasia | 1 1 1 1 1 1 1 1 | 15 1.1|
|__________________________________________________________________________|____________|
Parathyroid Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| 49 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 30
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3| |
| 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Pars Distalis, Angiectasis | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| 29 2.0|
Pars Distalis, Cyst | 1 2 2 1 2 2 1| 16 1.7|
Pars Distalis, Hyperplasia, Focal | 2 2 2 2 | 6 2.0|
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ultimobranchial Cyst | | 1 1.0|
C-Cell, Hyperplasia | 3 | 7 1.9|
Follicle, Cyst | 1 | 2 1.0|
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 1 2| 4 1.8|
Inflammation, Chronic | 2 | 2 3.0|
|__________________________________________________________________________|____________|
Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 1 3 3 2 | 9 2.0|
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | X | 2 |
Hydrometra | | 2 1.5|
|__________________________________________________________________________|____________|
Vagina | + | 1 |
Cyst | X | 1 |
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 3 2 2 4 | 9 2.6|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 31
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3| |
| 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Lymph Node | + + + + + | 11 |
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 | 8 2.0|
Pancreatic, Hyperplasia, Lymphoid | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M| |
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia, Lymphoid | | 1 1.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + A + + + + + + + + + +| 49 |
Hematopoietic Cell Proliferation | 4 2 4 | 7 2.7|
Capsule, Fibrosis | 1 1 1 1 | 12 1.1|
Lymphoid Follicle, Atrophy | | 1 3.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | X | 2 |
Galactocele | | 1 |
Hyperplasia, Atypical | 2 | 1 2.0|
Hyperplasia | 2 3 1 1 2 | 9 2.0|
Duct, Dilatation | 3 4 3 2 2 4 2 2 3 4 2 2 | 29 2.4|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation | 4 | 1 4.0|
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 32
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3| |
| 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
NERVOUS SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Compression | 2 | 6 2.2|
Hemorrhage | 4 | 1 4.0|
|__________________________________________________________________________|____________|
Peripheral Nerve | + + | 4 |
|__________________________________________________________________________|____________|
Spinal Cord | + + | 3 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Congestion | 1 1 1 | 5 1.0|
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 39 1.0|
Inflammation, Chronic | 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 29 1.0|
Inflammation, Suppurative | | 1 3.0|
Necrosis | | 1 3.0|
Alveolar Epithelium, Hyperplasia | 2 3 | 3 2.3|
|__________________________________________________________________________|____________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Foreign Body | 1 | 1 1.0|
Inflammation, Chronic | 2 1 1 1 | 9 1.8|
|__________________________________________________________________________|____________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cataract | 3 4 3 | 5 2.4|
Anterior Chamber, Inflammation | 2 | 1 2.0|
Cornea, Inflammation, Chronic | | 1 2.0|
Retina, Degeneration | 4 3 4 | 4 3.5|
|__________________________________________________________________________|____________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 33
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 5| 5| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 4| 9| 6| 3| 3| 3| 8| 3| 2| 3| 3| 2| 3| |
| 7| 7| 7| 7| 7| 4| 4| 4| 4| 7| 6| 6| 0| 6| 0| 6| 6| 6| 7| 6| 9| 6| 6| 3| 6| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
SPECIAL SENSES SYSTEM - cont | | |
Inflammation, Chronic | 1 2 | 3 1.3|
Pigmentation | | 1 2.0|
|__________________________________________________________________________|____________|
Zymbal's Gland | + | 1 |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 | 1 2.0|
Infarct | | 2 1.5|
Nephropathy | 3 1 1 1 1 1 1 1 1| 21 1.2|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 34
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|
DAY ON TEST | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|
| 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM | |
|__________________________________________________________________________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Colon | + + + + + + + + + + + + A + + + + + + + A + + + +|
Cyst | |
|__________________________________________________________________________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + A + + + +|
|__________________________________________________________________________|
Intestine Large, Cecum | A + + + + + + + + + + + A + + + A A + + A + + + +|
|__________________________________________________________________________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + A + + A + + + +|
Ectopic Tissue | |
|__________________________________________________________________________|
Intestine Small, Jejunum | A + + + A + + + + + + + + A + + A A + + A + + + +|
|__________________________________________________________________________|
Intestine Small, Ileum | + + + + + + + + + + + + A A + + A + + + A + + + +|
|__________________________________________________________________________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +|
Basophilic Focus | X X X X X X X X X X X X X X X X|
Clear Cell Focus | X X X |
Degeneration, Cystic | 1 |
Hepatodiaphragmatic Nodule | X 2 2 2 |
Inflammation, Chronic | 2 |
Bile Duct, Hyperplasia | 2 2 |
|__________________________________________________________________________|
Mesentery | + + + |
Fat, Necrosis | 2 2 3 |
|__________________________________________________________________________|
Oral Mucosa | + |
Pharyngeal, Cyst | 2 |
|__________________________________________________________________________|
Pancreas | + + + + + + + + + + + + + + + + + + + + A + + + +|
Atrophy | 1 1 2 4 1 2 1 1 2 2 2 2|
Acinus, Hyperplasia, Focal | |
|__________________________________________________________________________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + A + + + +|
__________________________________________|__________________________________________________________________________|
Page 35
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|
DAY ON TEST | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|
| 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM - cont | |
Erosion | |
Inflammation, Chronic Active | 1 |
Ulcer | 2 |
Epithelium, Hyperplasia | 2 1 |
|__________________________________________________________________________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + A + + + +|
|__________________________________________________________________________|
Tongue | |
Hyperplasia | |
_____________________________________________________________________________________________________________________|
CARDIOVASCULAR SYSTEM | |
|__________________________________________________________________________|
Blood Vessel | + |
|__________________________________________________________________________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cardiomyopathy | 1 1 1 1 1 2 1 1 1 2 1 2 2 1 |
_____________________________________________________________________________________________________________________|
ENDOCRINE SYSTEM | |
|__________________________________________________________________________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +|
Accessory Adrenal Cortical Nodule | 2 2 1 1 1 1 1 1 |
Degeneration, Fatty | 1 2 2 |
Hyperplasia, Focal | 2 2 1 2 |
Hypertrophy, Focal | 2 2 3 |
|__________________________________________________________________________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + A + + + +|
Hyperplasia | 1 1 1 1 1 1 1 1 |
|__________________________________________________________________________|
Parathyroid Gland | + + + + + + + + + M + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Pars Distalis, Angiectasis | 2 2 1 2 3 2 1 |
Pars Distalis, Cyst | 2 2 2 2 1 2 1 2 2 2 |
Pars Distalis, Hyperplasia, Focal | 2 1 2 |
|__________________________________________________________________________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
__________________________________________|__________________________________________________________________________|
Page 36
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|
DAY ON TEST | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|
| 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ENDOCRINE SYSTEM - cont | |
C-Cell, Hyperplasia | 2 |
Follicle, Cyst | 3 1 |
_____________________________________________________________________________________________________________________|
GENERAL BODY SYSTEM | |
None | |
_____________________________________________________________________________________________________________________|
GENITAL SYSTEM | |
|__________________________________________________________________________|
Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 2 2 2 2|
Inflammation, Chronic | 3 2 2 |
|__________________________________________________________________________|
Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 2 2 2 1 2 1 |
|__________________________________________________________________________|
Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | X |
Hemorrhage | 3 |
Hydrometra | 2 |
Endometrium, Hyperplasia, Cystic | 2 |
_____________________________________________________________________________________________________________________|
HEMATOPOIETIC SYSTEM | |
|__________________________________________________________________________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 2 |
|__________________________________________________________________________|
Lymph Node | + |
Mediastinal, Hyperplasia, Lymphoid | |
Pancreatic, Hyperplasia, Lymphoid | 2 |
|__________________________________________________________________________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M|
|__________________________________________________________________________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Spleen | + + + + + + + + + + + + + + + + + + + + A + + + +|
Hematopoietic Cell Proliferation | 2 2 1 1 |
Capsule, Fibrosis | 1 1 1 1 1 1 1 1 1 1 |
|__________________________________________________________________________|
__________________________________________|__________________________________________________________________________|
Page 37
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|
DAY ON TEST | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|
| 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
HEMATOPOIETIC SYSTEM - cont | |
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
INTEGUMENTARY SYSTEM | |
|__________________________________________________________________________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | |
Galactocele | X |
Hyperplasia | 2 |
Duct, Dilatation | 4 2 2 2 1 2 3 2 2 |
Duct, Hyperplasia | 2 |
|__________________________________________________________________________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
MUSCULOSKELETAL SYSTEM | |
|__________________________________________________________________________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
NERVOUS SYSTEM | |
|__________________________________________________________________________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +|
Compression | 2 |
|__________________________________________________________________________|
Peripheral Nerve | |
|__________________________________________________________________________|
Spinal Cord | |
_____________________________________________________________________________________________________________________|
RESPIRATORY SYSTEM | |
|__________________________________________________________________________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +|
Congestion | 1 1 2 2 |
Foreign Body | |
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 2 1 1 1 1 1|
Alveolar Epithelium, Hyperplasia | 2 3 |
|__________________________________________________________________________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | 2 1 |
Inflammation, Chronic | 2 2 2 |
__________________________________________|__________________________________________________________________________|
Page 38
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 5| 3| 7| 7| 2| 5| 7| 7| 6| 2| 2| 7| 7|
DAY ON TEST | 7| 2| 2| 2| 2| 3| 3| 3| 3| 4| 3| 3| 2| 4| 3| 3| 2| 7| 3| 3| 8| 8| 3| 3| 3|
| 7| 9| 9| 9| 5| 7| 7| 7| 7| 4| 6| 6| 7| 9| 6| 4| 3| 3| 4| 4| 7| 4| 9| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
RESPIRATORY SYSTEM - cont | |
|__________________________________________________________________________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
SPECIAL SENSES SYSTEM | |
|__________________________________________________________________________|
Ear | + |
|__________________________________________________________________________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cataract | |
Cornea, Inflammation, Chronic | |
Retina, Degeneration | |
|__________________________________________________________________________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 1 1 |
Pigmentation | 2 |
_____________________________________________________________________________________________________________________|
URINARY SYSTEM | |
|__________________________________________________________________________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 |
Infarct | 2 |
Nephropathy | 1 1 1 1 1 1 |
|__________________________________________________________________________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +|
__________________________________________|__________________________________________________________________________
Page 39
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
Cyst | 4 | 1 4.0|
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + A + + + + + + + + + + + + + + +| 44 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
Ectopic Tissue | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + A + + + + + + + + + + + + + + +| 43 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + A + + + + + + + + + + + +| 45 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Basophilic Focus | X X X X X X X X X X X X X X X X X X X X X X X X| 40 |
Clear Cell Focus | X X X X X| 8 |
Degeneration, Cystic | | 1 1.0|
Hepatodiaphragmatic Nodule | | 4 2.0|
Inflammation, Chronic | 2 1 2 2 | 5 1.8|
Bile Duct, Hyperplasia | | 2 2.0|
|__________________________________________________________________________|____________|
Mesentery | + + + + +| 8 |
Fat, Necrosis | 2 2 2 4 2| 8 2.4|
|__________________________________________________________________________|____________|
Oral Mucosa | | 1 |
Pharyngeal, Cyst | | 1 2.0|
|__________________________________________________________________________|____________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Atrophy | 1 1 1 1 1 1 2 3 1 1 1| 23 1.5|
Acinus, Hyperplasia, Focal | 2 | 1 2.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 40
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Erosion | 1 | 1 1.0|
Inflammation, Chronic Active | 1 | 2 1.0|
Ulcer | | 1 2.0|
Epithelium, Hyperplasia | 1 | 3 1.3|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Tongue | + | 1 |
Hyperplasia | 2 | 1 2.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Blood Vessel | + | 2 |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cardiomyopathy | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1| 32 1.2|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Accessory Adrenal Cortical Nodule | 2 1 1 1 | 12 1.3|
Degeneration, Fatty | 1 1 2 1 1| 8 1.4|
Hyperplasia, Focal | 2 3| 6 2.0|
Hypertrophy, Focal | 1 2 2 2 | 7 2.0|
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Hyperplasia | 1 1 1 1 1 1 1 | 15 1.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 41
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Pars Distalis, Angiectasis | 2 2 2 2 2 4 2 2 2 2 2 3 2 1| 21 2.0|
Pars Distalis, Cyst | 2 2 1 1 2 3 3 2 3 2 2 2 2| 23 2.0|
Pars Distalis, Hyperplasia, Focal | 2 2 2 | 6 1.8|
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
C-Cell, Hyperplasia | 2 1 3 2 | 5 2.0|
Follicle, Cyst | 2 2 | 4 2.0|
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 | 6 2.0|
Inflammation, Chronic | 2 | 4 2.3|
|__________________________________________________________________________|____________|
Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 3 2 1 | 10 1.8|
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | X X | 3 |
Hemorrhage | | 1 3.0|
Hydrometra | | 1 2.0|
Endometrium, Hyperplasia, Cystic | 2 | 2 2.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 4 2 3 | 6 2.5|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 42
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM - cont | | |
Lymph Node | + +| 3 |
Mediastinal, Hyperplasia, Lymphoid | 2 2| 2 2.0|
Pancreatic, Hyperplasia, Lymphoid | | 1 2.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M| |
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Hematopoietic Cell Proliferation | 2 2 2 1 | 8 1.6|
Capsule, Fibrosis | 1 1 1 1 1 1 1 1 1 1| 20 1.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 1 | 1 1.0|
Galactocele | | 1 |
Hyperplasia | 2 2 2 4 2 4 | 7 2.6|
Duct, Dilatation | 2 2 2 2 1 4 2 2 3 2 4 3 2 4 2| 24 2.4|
Duct, Hyperplasia | | 1 2.0|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Compression | 2 2 2 2 | 5 2.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 43
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
NERVOUS SYSTEM - cont | | |
Peripheral Nerve | + + | 2 |
|__________________________________________________________________________|____________|
Spinal Cord | + + | 2 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Congestion | 1 2 2 1 | 8 1.5|
Foreign Body | 3 | 1 3.0|
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 38 1.0|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 33 1.0|
Alveolar Epithelium, Hyperplasia | | 2 2.5|
|__________________________________________________________________________|____________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Foreign Body | 2 | 3 1.7|
Inflammation, Chronic | 2 1 1 | 6 1.7|
|__________________________________________________________________________|____________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Ear | + + | 3 |
|__________________________________________________________________________|____________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cataract | 2 2 2 | 3 2.0|
Cornea, Inflammation, Chronic | 2 3 | 2 2.5|
Retina, Degeneration | 4 4 | 2 4.0|
|__________________________________________________________________________|____________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 1 1 | 4 1.0|
Pigmentation | | 1 2.0|
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 44
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 7| 7| 7| 5| 6| 4| 7| 3| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 3| 2| 2| 2| 0| 7| 8| 3| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 7| 7| 7| 7| 7| 9| 9| 9| 9| 7| 0| 7| 2| 8| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
URINARY SYSTEM - cont | | |
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | | 1 2.0|
Infarct | 2 | 2 2.0|
Nephropathy | 1 1 1 1 1 1 1 1 1 1| 16 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 45
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|
DAY ON TEST | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|
| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM | |
|__________________________________________________________________________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ectopic Tissue | |
|__________________________________________________________________________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + A + +|
|__________________________________________________________________________|
Intestine Small, Ileum | + + + + + + + A + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +|
Basophilic Focus | X X X X |
Clear Cell Focus | X X X X X X X X|
Degeneration, Cystic | 1 |
Eosinophilic Focus | X X X X |
Hepatodiaphragmatic Nodule | X 2 3 2 2|
Necrosis, Focal | |
Bile Duct, Hyperplasia | 2 1 2 2 3 2 4 2 3 2 3 2 2 3 3 3 3 3 3 2 3 2|
Centrilobular, Necrosis | 2 |
Hepatocyte, Vacuolization Cytoplasmic| 2 2 3 |
|__________________________________________________________________________|
Mesentery | + + + + + + + |
Fibrosis | |
Fat, Necrosis | 2 3 3 3 3 3 |
|__________________________________________________________________________|
Oral Mucosa | |
|__________________________________________________________________________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +|
Atrophy | 2 2 4 1 1 3 2 2 1 4 4 2 1 3 2 1 2 2 2 1 2|
Acinus, Hyperplasia, Focal | 2 |
|__________________________________________________________________________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +|
__________________________________________|__________________________________________________________________________|
Page 46
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|
DAY ON TEST | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|
| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM - cont | |
|__________________________________________________________________________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 2 4 |
Erosion | 2 2 3 |
Ulcer | 4 |
Epithelium, Hyperplasia | 2 2 3 |
|__________________________________________________________________________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 |
Erosion | 2 2 |
Hyperplasia | 2 |
Inflammation | 2 |
Ulcer | 3 |
_____________________________________________________________________________________________________________________|
CARDIOVASCULAR SYSTEM | |
|__________________________________________________________________________|
Blood Vessel | + + |
|__________________________________________________________________________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cardiomyopathy | 1 1 2 2 2 2 2 1 2 2 1 1 1 2 1 1 2 2 2 2 1 2 2|
Pericardium, Inflammation, | |
Granulomatous | 3 |
_____________________________________________________________________________________________________________________|
ENDOCRINE SYSTEM | |
|__________________________________________________________________________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +|
Accessory Adrenal Cortical Nodule | 1 1 1 1 1 1 1 1 2 1 |
Degeneration, Fatty | 2 1 |
Hyperplasia, Focal | 1 1 1 1 |
Hypertrophy, Focal | 3 2 2 |
Necrosis | |
|__________________________________________________________________________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 2 2 3 2 |
|__________________________________________________________________________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 1 1 2 1 1|
|__________________________________________________________________________|
__________________________________________|__________________________________________________________________________|
Page 47
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|
DAY ON TEST | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|
| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ENDOCRINE SYSTEM - cont | |
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Pars Distalis, Angiectasis | 3 3 2 2 2 2 |
Pars Distalis, Cyst | X 4 2 2 |
Pars Distalis, Hyperplasia, Focal | 2 2 2 |
Pars Intermedia, Cyst | |
|__________________________________________________________________________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ultimobranchial Cyst | 2 |
C-Cell, Hyperplasia | 2 3 3 |
Follicle, Cyst | |
_____________________________________________________________________________________________________________________|
GENERAL BODY SYSTEM | |
|__________________________________________________________________________|
Peritoneum | |
_____________________________________________________________________________________________________________________|
GENITAL SYSTEM | |
|__________________________________________________________________________|
Coagulating Gland | |
|__________________________________________________________________________|
Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Penis | |
Fibrosis | |
|__________________________________________________________________________|
Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | X X 3 |
Inflammation, Chronic | 1 2 3 |
|__________________________________________________________________________|
Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 3 2 2 4 2 2 1 2 2 3 2 2 1 2 1 2 2|
|__________________________________________________________________________|
Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Testes | + + + + + + + + + + + + + + + + + + + + + + + + +|
Bilateral, Germinal Epithelium, | |
Atrophy | 4 |
__________________________________________|__________________________________________________________________________|
Page 48
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|
DAY ON TEST | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|
| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
GENITAL SYSTEM - cont | |
Germinal Epithelium, Atrophy | 1 |
_____________________________________________________________________________________________________________________|
HEMATOPOIETIC SYSTEM | |
|__________________________________________________________________________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 3 2 3 2 2 |
|__________________________________________________________________________|
Lymph Node | + + + + + + + + + + + + |
Deep Cervical, Inflammation, | |
Granulomatous | 2 |
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 2 |
Mediastinal, Inflammation, | |
Granulomatous | 3 |
Pancreatic, Hyperplasia, Lymphoid | 2 |
|__________________________________________________________________________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M|
|__________________________________________________________________________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia, Lymphoid | 2 2 1 2 2 |
|__________________________________________________________________________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +|
Fibrosis | 2 |
Hematopoietic Cell Proliferation | 2 1 1 3 2 1 |
Necrosis | |
Capsule, Fibrosis | |
|__________________________________________________________________________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
INTEGUMENTARY SYSTEM | |
|__________________________________________________________________________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | X X X X X X X X X 1 1 2 2 |
Hyperplasia | |
|__________________________________________________________________________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +|
Lip, Inflammation | |
_____________________________________________________________________________________________________________________|
MUSCULOSKELETAL SYSTEM | |
__________________________________________|__________________________________________________________________________|
Page 49
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|
DAY ON TEST | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|
| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
MUSCULOSKELETAL SYSTEM - cont | |
|__________________________________________________________________________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | |
|__________________________________________________________________________|
Skeletal Muscle | |
_____________________________________________________________________________________________________________________|
NERVOUS SYSTEM | |
|__________________________________________________________________________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +|
Compression | 2 3 3 3 |
Hemorrhage | 3 |
|__________________________________________________________________________|
Peripheral Nerve | + + |
|__________________________________________________________________________|
Spinal Cord | + |
_____________________________________________________________________________________________________________________|
RESPIRATORY SYSTEM | |
|__________________________________________________________________________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +|
Congestion | 2 2 |
Hemorrhage | 2 |
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1|
Inflammation, Chronic | 1 1 1 1 1 3 1 1 1|
Alveolar Epithelium, Hyperplasia | 2 2 2 4|
Mediastinum, Inflammation, | |
Granulomatous | 3 |
|__________________________________________________________________________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | 2 2 X 2 |
Inflammation, Chronic | 3 2 2 1 2 3 2 2|
|__________________________________________________________________________|
Pleura | + |
Inflammation, Granulomatous | 3 |
|__________________________________________________________________________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation | 3 |
_____________________________________________________________________________________________________________________|
SPECIAL SENSES SYSTEM | |
__________________________________________|__________________________________________________________________________|
Page 50
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 7| 7| 4| 7| 6| 7| 7| 6| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 7| 7|
DAY ON TEST | 3| 3| 6| 3| 3| 3| 3| 5| 3| 4| 3| 3| 6| 0| 3| 6| 3| 7| 3| 3| 3| 9| 4| 3| 3|
| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 1| 2| 2| 0| 2| 3| 5| 2| 2| 2| 4| 2| 3| 3|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
SPECIAL SENSES SYSTEM - cont | |
|__________________________________________________________________________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cataract | |
Inflammation, Chronic | |
Retina, Degeneration | |
|__________________________________________________________________________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 2 |
Pigmentation | 2 |
_____________________________________________________________________________________________________________________|
URINARY SYSTEM | |
|__________________________________________________________________________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +|
Infarct | 1 2 |
Nephropathy | 3 2 1 3 3 2 1 1 1 1 1 1 1 2 1 2 2 2 2 |
|__________________________________________________________________________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hemorrhage | 2 |
__________________________________________|__________________________________________________________________________
Page 51
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3| |
| 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + A + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ectopic Tissue | X | 1 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + A A + + + + + + + + + + + + + + +| 47 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + A + + + + + + + + + + + + + + + +| 48 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Basophilic Focus | X | 5 |
Clear Cell Focus | X X X X X X | 14 |
Degeneration, Cystic | 2 2 2 2 | 5 1.8|
Eosinophilic Focus | X | 5 |
Hepatodiaphragmatic Nodule | 2 2 2 2 | 9 2.1|
Necrosis, Focal | 2 | 1 2.0|
Bile Duct, Hyperplasia | 3 2 4 2 2 2 2 2 2 2 3 2 2 2 3 4 2 3 3| 41 2.5|
Centrilobular, Necrosis | 1 | 2 1.5|
Hepatocyte, Vacuolization Cytoplasmic| 1 3 | 5 2.2|
|__________________________________________________________________________|____________|
Mesentery | + + + + + + + + + | 16 |
Fibrosis | 2 | 1 2.0|
Fat, Necrosis | 2 3 3 2 3 2 2 2 | 14 2.6|
|__________________________________________________________________________|____________|
Oral Mucosa | + | 1 |
|__________________________________________________________________________|____________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 52
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3| |
| 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM - cont | | |
Atrophy | 2 1 4 2 2 1 1 1 2 1 2 1 3 2 3 2 3 4 1 1 2 4| 43 2.1|
Acinus, Hyperplasia, Focal | 2 2 2 | 4 2.0|
|__________________________________________________________________________|____________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Edema | 2 3 2 | 6 2.5|
Erosion | | 3 2.3|
Ulcer | 2 4 4 | 4 3.5|
Epithelium, Hyperplasia | 2 | 4 2.3|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Edema | | 1 2.0|
Erosion | 2 2 | 4 2.0|
Hyperplasia | | 1 2.0|
Inflammation | | 1 2.0|
Ulcer | 4 | 2 3.5|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Blood Vessel | + + | 4 |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cardiomyopathy | 2 2 2 2 1 2 1 2 2 1 2 2 1 1 1 1 1 2 2 2 2 2 2 2| 47 1.6|
Pericardium, Inflammation, | | |
Granulomatous | | 1 3.0|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Accessory Adrenal Cortical Nodule | 1 1 1 1 1 1 1 1 1 1 1 1 2| 23 1.1|
Degeneration, Fatty | 1 2 2 3 2| 7 1.9|
Hyperplasia, Focal | 1 2| 6 1.2|
Hypertrophy, Focal | 2 3 2| 6 2.3|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 53
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3| |
| 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
Necrosis | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 1 2 | 7 2.0|
|__________________________________________________________________________|____________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 1 1 2 1 1 1 2 1 | 13 1.2|
|__________________________________________________________________________|____________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Pars Distalis, Angiectasis | 2 2 2 2 2 2 2 2 | 14 2.1|
Pars Distalis, Cyst | 2 2 | 6 2.4|
Pars Distalis, Hyperplasia, Focal | 3 2 2 2 2 2 | 9 2.1|
Pars Intermedia, Cyst | 1| 1 1.0|
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ultimobranchial Cyst | 2 1 | 3 1.7|
C-Cell, Hyperplasia | 3 3 1 2 2| 8 2.4|
Follicle, Cyst | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
|__________________________________________________________________________|____________|
Peritoneum | + | 1 |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Coagulating Gland | + | 1 |
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Penis | + | 1 |
Fibrosis | 2 | 1 2.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 54
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3| |
| 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
GENITAL SYSTEM - cont | | |
Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 | 4 2.5|
Inflammation, Chronic | 2 2 | 5 2.0|
|__________________________________________________________________________|____________|
Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 1 2 2 3 4 1 1 2 3 3 1 1 1 3 | 31 2.0|
|__________________________________________________________________________|____________|
Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Bilateral, Germinal Epithelium, | | |
Atrophy | | 1 4.0|
Germinal Epithelium, Atrophy | | 1 1.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 3 | 7 2.4|
|__________________________________________________________________________|____________|
Lymph Node | + + + + + + + + + | 21 |
Deep Cervical, Inflammation, | | |
Granulomatous | | 1 2.0|
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 | 9 2.0|
Mediastinal, Inflammation, | | |
Granulomatous | | 1 3.0|
Pancreatic, Hyperplasia, Lymphoid | 2 | 2 2.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | M M M M + M M M M M M M M M M M M M M M M M M M M| 1 |
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia, Lymphoid | 4 3 | 7 2.3|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Fibrosis | | 1 2.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 55
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3| |
| 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM - cont | | |
Hematopoietic Cell Proliferation | 2 2 1 1 1 | 11 1.5|
Necrosis | 2 4 | 2 3.0|
Capsule, Fibrosis | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + + + + M + + + + + +| 49 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 3 2 2 2 2 3 1 1 2 1 | 23 1.8|
Hyperplasia | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Lip, Inflammation | 4 | 1 4.0|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | X | 1 |
|__________________________________________________________________________|____________|
Skeletal Muscle | + + + | 3 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Compression | 3 3 4 | 7 3.0|
Hemorrhage | | 1 3.0|
|__________________________________________________________________________|____________|
Peripheral Nerve | + + + + | 6 |
|__________________________________________________________________________|____________|
Spinal Cord | + + + | 4 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 56
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3| |
| 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
RESPIRATORY SYSTEM - cont | | |
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Congestion | 1 | 3 1.7|
Hemorrhage | | 1 2.0|
Infiltration Cellular, Histiocyte | 2 1 1 1 1 1 1 1 1 1 2 1| 28 1.1|
Inflammation, Chronic | 1 1 2 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1| 29 1.1|
Alveolar Epithelium, Hyperplasia | 2 1 1 | 7 2.0|
Mediastinum, Inflammation, | | |
Granulomatous | | 1 3.0|
|__________________________________________________________________________|____________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Foreign Body | 2 2 2 | 7 2.0|
Inflammation, Chronic | 2 2 2 1 | 12 2.0|
|__________________________________________________________________________|____________|
Pleura | | 1 |
Inflammation, Granulomatous | | 1 3.0|
|__________________________________________________________________________|____________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation | | 1 3.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cataract | 2 | 1 2.0|
Inflammation, Chronic | 4 | 1 4.0|
Retina, Degeneration | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 2 | 2 2.0|
Pigmentation | 2 | 2 2.0|
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Infarct | | 2 1.5|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 57
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 6| 6| 6| 7| 7| 6| 5| 6| 5| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 2| 4| 9| 3| 2| 8| 8| 4| 8| 3| 3| 1| 3| 3| 3| 1| 3| 4| 3| 3| 3| 3| |
| 3| 3| 1| 0| 1| 4| 0| 9| 5| 6| 9| 6| 0| 0| 2| 2| 2| 0| 6| 0| 4| 2| 2| 0| 0| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A |
0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
URINARY SYSTEM - cont | | |
Nephropathy | 3 2 1 1 3 2 1 1 1 1 2 2 3 1 2 3 | 35 1.7|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hemorrhage | | 1 2.0|
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 58
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|
DAY ON TEST | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|
| 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM | |
|__________________________________________________________________________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ectopic Tissue | 2 |
|__________________________________________________________________________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + A A + + + + + + + A|
|__________________________________________________________________________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +|
Basophilic Focus | X X X |
Clear Cell Focus | X X X X X X X |
Degeneration, Cystic | 1 |
Eosinophilic Focus | |
Hepatodiaphragmatic Nodule | 2 2 |
Inflammation, Chronic | |
Necrosis, Focal | 1 |
Bile Duct, Hyperplasia | 3 2 2 2 1 2 2 2 2 2 2 3 2 2 3 2 2 |
Hepatocyte, Vacuolization Cytoplasmic| 2 |
|__________________________________________________________________________|
Mesentery | + + + |
Fat, Necrosis | 2 2 3 |
|__________________________________________________________________________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +|
Atrophy | 2 2 3 1 2 1 2 2 2 1 2 1 3 1|
Acinus, Hyperplasia, Focal | 1 2 2 1 |
|__________________________________________________________________________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ulcer | 2 |
__________________________________________|__________________________________________________________________________|
Page 59
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|
DAY ON TEST | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|
| 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM - cont | |
|__________________________________________________________________________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +|
Erosion | 3 3 |
Mineralization | 2 |
Ulcer | 2 |
_____________________________________________________________________________________________________________________|
CARDIOVASCULAR SYSTEM | |
|__________________________________________________________________________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cardiomyopathy | 2 1 2 1 1 2 2 2 2 1 1 2 1 1 1 2 1 1 1 2 1 1 1 1 2|
Mineralization | 3 |
Thrombosis | 2 |
_____________________________________________________________________________________________________________________|
ENDOCRINE SYSTEM | |
|__________________________________________________________________________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +|
Accessory Adrenal Cortical Nodule | 2 1 1 1 1 1 1 1 1 2 1 |
Degeneration, Fatty | 1 |
Hyperplasia, Focal | 2 1 |
Hypertrophy, Focal | 1 |
|__________________________________________________________________________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 1 2 2 2 2 1 2 2 |
|__________________________________________________________________________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 2 1 2 1 2 1 4 1 |
|__________________________________________________________________________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Pars Distalis, Angiectasis | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2|
Pars Distalis, Cyst | 1 1 2 |
Pars Distalis, Hyperplasia, Focal | 2 2 3 1 |
Pars Distalis, Hyperplasia, | |
Multifocal | |
|__________________________________________________________________________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ultimobranchial Cyst | 1 |
__________________________________________|__________________________________________________________________________|
Page 60
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|
DAY ON TEST | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|
| 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ENDOCRINE SYSTEM - cont | |
C-Cell, Hyperplasia | 1 |
Follicle, Cyst | 2 |
_____________________________________________________________________________________________________________________|
GENERAL BODY SYSTEM | |
|__________________________________________________________________________|
Peritoneum | |
_____________________________________________________________________________________________________________________|
GENITAL SYSTEM | |
|__________________________________________________________________________|
Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 2 |
|__________________________________________________________________________|
Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 2 2 2 2 2 2 3 1 2 2 1 2 1 |
|__________________________________________________________________________|
Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Testes | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
HEMATOPOIETIC SYSTEM | |
|__________________________________________________________________________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 4 3 3 2 |
|__________________________________________________________________________|
Lymph Node | + + + + + + +|
Deep Cervical, Hyperplasia, Lymphoid | 3 |
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2|
Pancreatic, Hyperplasia, Lymphoid | 2 |
|__________________________________________________________________________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M|
|__________________________________________________________________________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia, Lymphoid | 2 2|
|__________________________________________________________________________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +|
Fibrosis | 2 1 |
__________________________________________|__________________________________________________________________________|
Page 61
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|
DAY ON TEST | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|
| 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
HEMATOPOIETIC SYSTEM - cont | |
Hematopoietic Cell Proliferation | 3 3 1 2 1 4 1 2|
Capsule, Fibrosis | 1 1 |
Lymphoid Follicle, Hyperplasia | |
|__________________________________________________________________________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
INTEGUMENTARY SYSTEM | |
|__________________________________________________________________________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 3 1 2 2 2 1 1 |
|__________________________________________________________________________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
MUSCULOSKELETAL SYSTEM | |
|__________________________________________________________________________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cranium, Osteopetrosis | 2 |
|__________________________________________________________________________|
Skeletal Muscle | |
_____________________________________________________________________________________________________________________|
NERVOUS SYSTEM | |
|__________________________________________________________________________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +|
Compression | 2 2 2 2 2 2 |
Hemorrhage | 1 2 |
|__________________________________________________________________________|
Peripheral Nerve | |
|__________________________________________________________________________|
Spinal Cord | |
_____________________________________________________________________________________________________________________|
RESPIRATORY SYSTEM | |
|__________________________________________________________________________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +|
Congestion | 2 1 |
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 |
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 |
Alveolar Epithelium, Hyperplasia | 1 2 3 |
|__________________________________________________________________________|
__________________________________________|__________________________________________________________________________|
Page 62
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 6| 7| 6| 7| 5| 4| 7| 7| 7| 6| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 5|
DAY ON TEST | 7| 3| 8| 3| 7| 7| 3| 3| 3| 5| 3| 3| 3| 3| 3| 6| 2| 3| 3| 3| 3| 3| 3| 3| 4|
| 3| 2| 2| 0| 6| 3| 2| 2| 2| 2| 2| 2| 1| 1| 1| 1| 0| 2| 2| 2| 2| 2| 2| 2| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
5 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
RESPIRATORY SYSTEM - cont | |
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | 1 |
Inflammation, Chronic | 1 |
|__________________________________________________________________________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
SPECIAL SENSES SYSTEM | |
|__________________________________________________________________________|
Eye | + + + + + + + + + + + + + + + A + + + + + + + + +|
Cataract | 3 |
Bilateral, Anterior Chamber, | |
Inflammation, Acute | 2 |
Retina, Degeneration | 3 |
|__________________________________________________________________________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 1 1 1 |
Pigmentation | |
|__________________________________________________________________________|
Zymbal's Gland | |
_____________________________________________________________________________________________________________________|
URINARY SYSTEM | |
|__________________________________________________________________________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hydronephrosis | 3 |
Nephropathy | 2 2 3 1 1 1 3 1 2 2 3 1 1 2 1 1 1 1|
|__________________________________________________________________________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 4 |
__________________________________________|__________________________________________________________________________
Page 63
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5| |
DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6| |
| 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ectopic Tissue | | 1 2.0|
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Basophilic Focus | X X X | 6 |
Clear Cell Focus | X X X X X X| 13 |
Degeneration, Cystic | 2 1 | 3 1.3|
Eosinophilic Focus | X | 1 |
Hepatodiaphragmatic Nodule | | 2 2.0|
Inflammation, Chronic | 1 | 1 1.0|
Necrosis, Focal | | 1 1.0|
Bile Duct, Hyperplasia | 3 3 2 2 2 2 4 2 2 2 3 3 2 2 2 2 2 3 | 35 2.3|
Hepatocyte, Vacuolization Cytoplasmic| | 1 2.0|
|__________________________________________________________________________|____________|
Mesentery | + + + + + + + + | 11 |
Fat, Necrosis | 2 3 2 1 3 3 | 9 2.3|
|__________________________________________________________________________|____________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Atrophy | 1 1 2 2 1 2 1 4 1 1 2 4 2 1 2 3 2| 31 1.8|
Acinus, Hyperplasia, Focal | 2 2| 6 1.7|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 64
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5| |
DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6| |
| 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM - cont | | |
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ulcer | | 1 2.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Erosion | | 2 3.0|
Mineralization | | 1 2.0|
Ulcer | | 1 2.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cardiomyopathy | 2 2 1 2 2 1 1 2 2 2 2 1 2 1 2 1 1 1 1 1 2| 46 1.5|
Mineralization | | 1 3.0|
Thrombosis | | 1 2.0|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Accessory Adrenal Cortical Nodule | 2 2 1 1 1 1 1 1 1 1 1 1 | 23 1.2|
Degeneration, Fatty | 1 1 1 2 2 | 6 1.3|
Hyperplasia, Focal | 1 2 2 | 5 1.6|
Hypertrophy, Focal | 1 2 2 2 | 5 1.6|
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 1 3 2 1 | 12 1.8|
|__________________________________________________________________________|____________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 1 1 2 | 13 1.7|
|__________________________________________________________________________|____________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 65
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5| |
DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6| |
| 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
Pars Distalis, Angiectasis | 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 | 30 2.0|
Pars Distalis, Cyst | | 3 1.3|
Pars Distalis, Hyperplasia, Focal | 2 1 2 3 | 8 2.0|
Pars Distalis, Hyperplasia, | | |
Multifocal | 3 | 1 3.0|
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ultimobranchial Cyst | | 1 1.0|
C-Cell, Hyperplasia | 3 | 2 2.0|
Follicle, Cyst | 2 | 2 2.0|
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
|__________________________________________________________________________|____________|
Peritoneum | + | 1 |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 1 1 2 1 | 5 1.4|
|__________________________________________________________________________|____________|
Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 2 1 2 2 2 2 1 2 1 2 2 2 2 1 2 | 28 1.8|
|__________________________________________________________________________|____________|
Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 3 3| 7 2.9|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 66
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5| |
DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6| |
| 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM - cont | | |
Lymph Node | + + + + + + + + + + | 17 |
Deep Cervical, Hyperplasia, Lymphoid | | 1 3.0|
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 2 | 9 2.0|
Pancreatic, Hyperplasia, Lymphoid | 2 2 2 | 4 2.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | M M + M M M M M M M M M M M M M M M M M M M M M M| 1 |
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia, Lymphoid | 2 | 3 2.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Fibrosis | | 2 1.5|
Hematopoietic Cell Proliferation | 2 1 1 3| 12 2.0|
Capsule, Fibrosis | 1 2 2 1 1 | 7 1.3|
Lymphoid Follicle, Hyperplasia | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 1 3 1 2 1 1 2 1 2 | 18 1.7|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cranium, Osteopetrosis | | 1 2.0|
|__________________________________________________________________________|____________|
Skeletal Muscle | + | 1 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 67
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5| |
DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6| |
| 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
NERVOUS SYSTEM - cont | | |
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Compression | 2 2 2 2 | 10 2.0|
Hemorrhage | | 2 1.5|
|__________________________________________________________________________|____________|
Peripheral Nerve | + + + | 3 |
|__________________________________________________________________________|____________|
Spinal Cord | + | 1 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Congestion | 1 2 | 4 1.5|
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 2 1 1 | 29 1.0|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 | 23 1.0|
Alveolar Epithelium, Hyperplasia | 2 4 2 2 2 2 | 9 2.2|
|__________________________________________________________________________|____________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Foreign Body | 1 1 1 | 4 1.0|
Inflammation, Chronic | 1 2 | 3 1.3|
|__________________________________________________________________________|____________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
Cataract | 3 2 | 3 2.7|
Bilateral, Anterior Chamber, | | |
Inflammation, Acute | | 1 2.0|
Retina, Degeneration | 3 | 2 3.0|
|__________________________________________________________________________|____________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | | 3 1.0|
Pigmentation | 2 | 1 2.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 68
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 5| 5| 7| 5| |
DAY ON TEST | 5| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 7| 3| 4| 3| 2| 8| 9| 1| 6| |
| 2| 0| 0| 0| 0| 0| 0| 0| 3| 3| 3| 1| 1| 1| 0| 0| 3| 0| 0| 0| 2| 2| 0| 2| 8| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A |
5 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
SPECIAL SENSES SYSTEM - cont | | |
Zymbal's Gland | + | 1 |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hydronephrosis | | 1 3.0|
Nephropathy | 3 2 2 1 1 3 2 1 1 3 2 2 2 1 4 4 1 2 1 1 1| 39 1.8|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 2 | 2 3.0|
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 69
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|
DAY ON TEST | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|
| 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM | |
|__________________________________________________________________________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Cecum | + + + + + + A + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ulcer | 2 |
|__________________________________________________________________________|
Intestine Small, Jejunum | + + + + + + A + + + + + + + + + + + + + + + A + A|
|__________________________________________________________________________|
Intestine Small, Ileum | + + + + + + A + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +|
Basophilic Focus | X X X |
Clear Cell Focus | X X X X X X X |
Degeneration, Cystic | 1 |
Eosinophilic Focus | X X X |
Hepatodiaphragmatic Nodule | |
Bile Duct, Hyperplasia | 3 2 2 3 1 2 2 2 1 1 2 2 2 2 2 2 2|
Centrilobular, Necrosis | |
Hepatocyte, Vacuolization Cytoplasmic| 3 2|
|__________________________________________________________________________|
Mesentery | + + + +|
Fat, Necrosis | 3 2 3 3|
|__________________________________________________________________________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +|
Atrophy | 4 1 2 2 2 2 3 2 4 1 2 2 2 2 2 1 1 1 |
Acinus, Hyperplasia, Focal | 2 2 1 2 2 2 1 |
|__________________________________________________________________________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 |
Ulcer | 3 2|
__________________________________________|__________________________________________________________________________|
Page 70
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|
DAY ON TEST | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|
| 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM - cont | |
Epithelium, Hyperplasia | 2 2|
|__________________________________________________________________________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +|
Erosion | 3 |
Ulcer | 2 2|
_____________________________________________________________________________________________________________________|
CARDIOVASCULAR SYSTEM | |
|__________________________________________________________________________|
Blood Vessel | |
|__________________________________________________________________________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cardiomyopathy | 2 2 1 1 1 2 1 1 1 2 1 1 1 1 1 2 2 2 2 1 2 1 1 1|
Thrombosis | |
_____________________________________________________________________________________________________________________|
ENDOCRINE SYSTEM | |
|__________________________________________________________________________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +|
Accessory Adrenal Cortical Nodule | 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 |
Degeneration, Fatty | 2 1 2|
Hyperplasia, Focal | 2 |
Hypertrophy, Focal | 2 2 2 2 |
|__________________________________________________________________________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 1 1 2 |
Thrombosis | |
|__________________________________________________________________________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 2 2 1 2 2 1 1 |
Metaplasia, Hepatocyte | 2 |
|__________________________________________________________________________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Pars Distalis, Angiectasis | 2 2 2 2 2 3 2 |
Pars Distalis, Cyst | 1 2 3 1|
Pars Distalis, Hyperplasia, Focal | 1 2 1 2 |
|__________________________________________________________________________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
__________________________________________|__________________________________________________________________________|
Page 71
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|
DAY ON TEST | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|
| 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ENDOCRINE SYSTEM - cont | |
C-Cell, Hyperplasia | 2 2 2 1 2 1 |
Follicle, Cyst | 1 1 |
Follicular Cell, Hyperplasia | |
_____________________________________________________________________________________________________________________|
GENERAL BODY SYSTEM | |
|__________________________________________________________________________|
Peritoneum | |
_____________________________________________________________________________________________________________________|
GENITAL SYSTEM | |
|__________________________________________________________________________|
Coagulating Gland | + |
Inflammation | 4 |
|__________________________________________________________________________|
Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 1 2 |
|__________________________________________________________________________|
Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 1 2 1 2 3 3 4 2 2 2 1 2 1 2 4 1 |
|__________________________________________________________________________|
Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Testes | + + + + + + + + + + + + + + + + + + + + + + + + +|
Bilateral, Germinal Epithelium, | |
Atrophy | 2 4 |
_____________________________________________________________________________________________________________________|
HEMATOPOIETIC SYSTEM | |
|__________________________________________________________________________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 4 2 |
|__________________________________________________________________________|
Lymph Node | + + + + + |
Deep Cervical, Hyperplasia, Lymphoid | 4 |
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 2 |
Pancreatic, Hyperplasia, Lymphoid | |
|__________________________________________________________________________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M|
__________________________________________|__________________________________________________________________________|
Page 72
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|
DAY ON TEST | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|
| 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
HEMATOPOIETIC SYSTEM - cont | |
|__________________________________________________________________________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia, Lymphoid | 2 |
|__________________________________________________________________________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +|
Fibrosis | |
Hematopoietic Cell Proliferation | 1 1 3 1 4 1 1 1 |
Capsule, Fibrosis | 1 2 1 1 2 1 |
Lymphoid Follicle, Hyperplasia | |
|__________________________________________________________________________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
INTEGUMENTARY SYSTEM | |
|__________________________________________________________________________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 2 2 2 2 3 1 2 1 1 1|
Hyperplasia | |
|__________________________________________________________________________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 2 2 4 |
_____________________________________________________________________________________________________________________|
MUSCULOSKELETAL SYSTEM | |
|__________________________________________________________________________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
NERVOUS SYSTEM | |
|__________________________________________________________________________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +|
Compression | 2 2 2 2 2 |
|__________________________________________________________________________|
Peripheral Nerve | + + + + |
|__________________________________________________________________________|
Spinal Cord | + + + |
_____________________________________________________________________________________________________________________|
RESPIRATORY SYSTEM | |
|__________________________________________________________________________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +|
Congestion | 1 1 |
__________________________________________|__________________________________________________________________________|
Page 73
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 5| 6| 6| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 4| 7| 6|
DAY ON TEST | 3| 3| 3| 3| 3| 8| 3| 1| 9| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 8| 3| 9|
| 0| 0| 0| 2| 2| 6| 7| 9| 0| 1| 1| 1| 3| 3| 3| 9| 0| 0| 2| 2| 2| 0| 9| 0| 0|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
25 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
RESPIRATORY SYSTEM - cont | |
Foreign Body | 2 |
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 |
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 |
Necrosis | 3 |
Alveolar Epithelium, Hyperplasia | 3 2 1 |
|__________________________________________________________________________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | 2 2 1 2|
Inflammation, Chronic | 2 2 2 2 1 1 1 1 2|
|__________________________________________________________________________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation | 2 |
Perforation | 2 |
_____________________________________________________________________________________________________________________|
SPECIAL SENSES SYSTEM | |
|__________________________________________________________________________|
Eye | + + + + + + A + + + + + + + + + + + + + + + A + +|
Cataract | 2 3 2 4 |
Inflammation, Chronic | 2 |
Retina, Degeneration | 3 4 3 4 |
|__________________________________________________________________________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 |
Inflammation, Chronic | 1 |
Pigmentation | |
|__________________________________________________________________________|
Zymbal's Gland | + |
_____________________________________________________________________________________________________________________|
URINARY SYSTEM | |
|__________________________________________________________________________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | |
Nephropathy | 1 2 1 1 3 1 1 1 1 1 1 2 1 1 |
|__________________________________________________________________________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 3 |
__________________________________________|__________________________________________________________________________
Page 74
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ulcer | | 1 2.0|
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Basophilic Focus | X X X X X| 8 |
Clear Cell Focus | X X X X X X X X X | 16 |
Degeneration, Cystic | 2 | 2 1.5|
Eosinophilic Focus | X | 4 |
Hepatodiaphragmatic Nodule | 2 X 3 | 3 2.5|
Bile Duct, Hyperplasia | 2 2 2 1 1 2 2 2 2 2 2 2 2 3 2 1 2 2 2 2 2| 38 1.9|
Centrilobular, Necrosis | 2 | 1 2.0|
Hepatocyte, Vacuolization Cytoplasmic| | 2 2.5|
|__________________________________________________________________________|____________|
Mesentery | + + + + + | 9 |
Fat, Necrosis | 3 4 3 3 | 8 3.0|
|__________________________________________________________________________|____________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Atrophy | 1 2 1 4 2 2 3 2 2 2 1 2 3 1 2 3 2| 35 2.0|
Acinus, Hyperplasia, Focal | 2 1 3 2 2 1 2 3 | 15 1.9|
|__________________________________________________________________________|____________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 75
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Edema | | 1 2.0|
Ulcer | | 2 2.5|
Epithelium, Hyperplasia | 1 | 3 1.7|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Erosion | | 1 3.0|
Ulcer | | 2 2.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Blood Vessel | + | 1 |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cardiomyopathy | 2 2 2 1 1 2 2 2 2 2 1 1 2 1 1 1 2 1 1 2 2 1 1| 47 1.4|
Thrombosis | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Accessory Adrenal Cortical Nodule | 1 1 1 2 1 2 2 1 1 1 1 3 2| 28 1.3|
Degeneration, Fatty | 2 2 2 1 | 7 1.7|
Hyperplasia, Focal | 1 1 2 2 | 5 1.6|
Hypertrophy, Focal | 1 2 2 2 2 | 9 1.9|
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 1 2 2 2 2| 8 1.6|
Thrombosis | 3 | 1 3.0|
|__________________________________________________________________________|____________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 2 2 2 3 2 2 2 1 | 17 1.8|
Metaplasia, Hepatocyte | | 1 2.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 76
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Pars Distalis, Angiectasis | 1 4 2 2 3 2 1 2 2| 16 2.1|
Pars Distalis, Cyst | 2 2 | 6 1.8|
Pars Distalis, Hyperplasia, Focal | 2 2 2 2 2 | 9 1.8|
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
C-Cell, Hyperplasia | 2 2 2 | 9 1.8|
Follicle, Cyst | 1 1 | 4 1.0|
Follicular Cell, Hyperplasia | 3 | 1 3.0|
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
|__________________________________________________________________________|____________|
Peritoneum | + | 1 |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Coagulating Gland | + | 2 |
Inflammation | | 1 4.0|
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 2 3| 4 2.0|
|__________________________________________________________________________|____________|
Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 2 2 1 2 2 2 2 2 1 2 2| 27 2.0|
|__________________________________________________________________________|____________|
Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Bilateral, Germinal Epithelium, | | |
Atrophy | | 2 3.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 77
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 | 4 2.5|
|__________________________________________________________________________|____________|
Lymph Node | + + + + + | 10 |
Deep Cervical, Hyperplasia, Lymphoid | | 1 4.0|
Mediastinal, Hyperplasia, Lymphoid | 2 2 4 2 2 | 10 2.2|
Pancreatic, Hyperplasia, Lymphoid | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M| |
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia, Lymphoid | 2 2 2 | 4 2.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Fibrosis | 2 2 | 2 2.0|
Hematopoietic Cell Proliferation | 1 2 4 1 1 1 1 1 1| 17 1.5|
Capsule, Fibrosis | 2 2 1 2 2 1 | 12 1.5|
Lymphoid Follicle, Hyperplasia | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 2 2 2 2 1 2 1 2| 20 1.8|
Hyperplasia | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 3 2 | 5 2.6|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 78
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Compression | 2 2 2 4 | 9 2.2|
|__________________________________________________________________________|____________|
Peripheral Nerve | | 4 |
|__________________________________________________________________________|____________|
Spinal Cord | | 3 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Congestion | 2 | 3 1.3|
Foreign Body | | 1 2.0|
Infiltration Cellular, Histiocyte | 1 1 1 2 2 1 2 1 1 1 1 1 1 1 1 1 1 1 1| 36 1.1|
Inflammation, Chronic | 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 | 36 1.0|
Necrosis | | 1 3.0|
Alveolar Epithelium, Hyperplasia | 3 | 4 2.3|
|__________________________________________________________________________|____________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Foreign Body | 2 1 1 2 2 2 2 1 | 12 1.7|
Inflammation, Chronic | 2 2 2 2 2 1 2 1 2 2 1 | 20 1.7|
|__________________________________________________________________________|____________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation | | 1 2.0|
Perforation | | 1 2.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
Cataract | | 4 2.8|
Inflammation, Chronic | | 1 2.0|
Retina, Degeneration | | 4 3.5|
|__________________________________________________________________________|____________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 79
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| |
DAY ON TEST | 3| 3| 3| 3| 1| 1| 3| 3| 3| 1| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| |
| 1| 1| 3| 3| 0| 9| 0| 0| 0| 2| 6| 1| 1| 1| 1| 1| 1| 0| 0| 0| 3| 3| 3| 1| 1| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A |
25 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
SPECIAL SENSES SYSTEM - cont | | |
Hyperplasia | | 1 2.0|
Inflammation, Chronic | | 1 1.0|
Pigmentation | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Zymbal's Gland | | 1 |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 3 | 1 3.0|
Nephropathy | 1 1 1 1 1 1 2 1 1 1 2 1 1 2 1 2 1 1 2| 33 1.3|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | | 1 3.0|
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 80
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|
DAY ON TEST | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|
| 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM | |
|__________________________________________________________________________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +|
Perforation | 3 4 |
|__________________________________________________________________________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation | |
|__________________________________________________________________________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Intestine Large, Cecum | + + A + + + + + + + + + + + + + + + + + + + + A +|
Inflammation, Chronic | 2 |
|__________________________________________________________________________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + A +|
|__________________________________________________________________________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + A +|
|__________________________________________________________________________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + A +|
Hyperplasia, Lymphoid | 3 |
|__________________________________________________________________________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +|
Basophilic Focus | X X 2 X|
Clear Cell Focus | X X X X X X X|
Degeneration, Cystic | 2 |
Eosinophilic Focus | X X |
Hepatodiaphragmatic Nodule | 2 2 |
Inflammation, Chronic | 1 |
Bile Duct, Hyperplasia | 2 2 2 3 2 2 2 2 2 1 1 1 2 2 3 2 2 2 2|
Centrilobular, Necrosis | |
Hepatocyte, Vacuolization Cytoplasmic| 3 |
Serosa, Inflammation | 2 |
|__________________________________________________________________________|
Mesentery | + + + |
Accessory Spleen | |
Fat, Necrosis | 2 2 |
|__________________________________________________________________________|
Oral Mucosa | + + |
Pharyngeal, Cyst | |
Pharyngeal, Inflammation | |
__________________________________________|__________________________________________________________________________|
Page 81
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|
DAY ON TEST | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|
| 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ALIMENTARY SYSTEM - cont | |
|__________________________________________________________________________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +|
Atrophy, Focal | |
Atrophy | 2 1 2 1 1 1 1 1 1 2 2 4 2 3 2 1 |
Cyst | 2 |
Inflammation, Chronic | 2 |
Acinus, Hyperplasia, Focal | 2 1 3 2 2 1 |
|__________________________________________________________________________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 |
|__________________________________________________________________________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | |
Erosion | 1 |
Ulcer | |
Epithelium, Hyperplasia | 1 |
|__________________________________________________________________________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +|
Edema | 2 |
Erosion | 2 |
Ulcer | 4 |
_____________________________________________________________________________________________________________________|
CARDIOVASCULAR SYSTEM | |
|__________________________________________________________________________|
Blood Vessel | |
|__________________________________________________________________________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cardiomyopathy | 2 1 1 4 2 1 1 2 2 2 3 2 2 1 1 2 2 2 2 2 1 1 1 |
_____________________________________________________________________________________________________________________|
ENDOCRINE SYSTEM | |
|__________________________________________________________________________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +|
Accessory Adrenal Cortical Nodule | 1 1 1 2 1 1 2 2 1 1 1 1 1 1 2 1|
Degeneration, Fatty | 2 |
Hyperplasia, Focal | 1 1 1 |
Hypertrophy, Focal | 2 2 |
Necrosis | 3 |
|__________________________________________________________________________|
__________________________________________|__________________________________________________________________________|
Page 82
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|
DAY ON TEST | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|
| 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
ENDOCRINE SYSTEM - cont | |
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 2 |
|__________________________________________________________________________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 1 1 1 1 1 1 1 2 3 2 1 1|
|__________________________________________________________________________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Pars Distalis, Angiectasis | 2 2 2 2 2 2 2 2 3 |
Pars Distalis, Cyst | 2 2 1 |
Pars Distalis, Hyperplasia, Focal | 2 2 2 3 3 2 2 |
|__________________________________________________________________________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Ultimobranchial Cyst | 2 1 |
C-Cell, Hyperplasia | 2 2 |
Follicle, Cyst | |
_____________________________________________________________________________________________________________________|
GENERAL BODY SYSTEM | |
|__________________________________________________________________________|
Peritoneum | |
_____________________________________________________________________________________________________________________|
GENITAL SYSTEM | |
|__________________________________________________________________________|
Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | |
Inflammation, Chronic | |
|__________________________________________________________________________|
Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +|
Inflammation, Chronic | 2 4 1 1 2 2 2 2 3 2 1 2 |
|__________________________________________________________________________|
Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Testes | + + + + + + + + + + + + + + + + + + + + + + + + +|
Germinal Epithelium, Atrophy | 3 |
__________________________________________|__________________________________________________________________________|
Page 83
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|
DAY ON TEST | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|
| 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
HEMATOPOIETIC SYSTEM | |
|__________________________________________________________________________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | 2 3 2 3 3 |
|__________________________________________________________________________|
Lymph Node | + + + + + + + + +|
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 2 2 2|
Pancreatic, Hyperplasia, Lymphoid | |
|__________________________________________________________________________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M|
|__________________________________________________________________________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia, Lymphoid | 2 4 2 2 2|
|__________________________________________________________________________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +|
Fibrosis | 2 |
Hematopoietic Cell Proliferation | 2 2 2 1 4 2 2 2 1 |
Hemorrhage | 2 |
Capsule, Fibrosis | 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 1 2|
|__________________________________________________________________________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +|
_____________________________________________________________________________________________________________________|
INTEGUMENTARY SYSTEM | |
|__________________________________________________________________________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Cyst | 2 1 2 3 2 2 1 2 3 |
|__________________________________________________________________________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperkeratosis | 2 |
Inflammation, Chronic | 2 |
Subcutaneous Tissue, Mineralization | |
_____________________________________________________________________________________________________________________|
MUSCULOSKELETAL SYSTEM | |
|__________________________________________________________________________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +|
|__________________________________________________________________________|
Skeletal Muscle | + |
_____________________________________________________________________________________________________________________|
NERVOUS SYSTEM | |
__________________________________________|__________________________________________________________________________|
Page 84
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|
DAY ON TEST | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|
| 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
NERVOUS SYSTEM - cont | |
|__________________________________________________________________________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +|
Compression | 3 2 1 |
|__________________________________________________________________________|
Peripheral Nerve | + + + +|
|__________________________________________________________________________|
Spinal Cord | + + + +|
_____________________________________________________________________________________________________________________|
RESPIRATORY SYSTEM | |
|__________________________________________________________________________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +|
Congestion | 2 2 2 1 2 |
Foreign Body | 2 2 |
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 |
Inflammation, Chronic | 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1|
Inflammation, Suppurative | |
Necrosis | 2 |
Alveolar Epithelium, Hyperplasia | 2 2 3 2 |
|__________________________________________________________________________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | 2 2 2 1|
Inflammation, Chronic | 2 2 2 2 2 1 2|
|__________________________________________________________________________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +|
Foreign Body | 2 |
Necrosis | 2 |
_____________________________________________________________________________________________________________________|
SPECIAL SENSES SYSTEM | |
|__________________________________________________________________________|
Eye | + + A + + + + + + + + + + + + + + + + + + + + A +|
Cataract | 3 3 |
Inflammation, Chronic | 4 |
Phthisis Bulbi | X|
Anterior Chamber, Inflammation, Acute| 2 |
Retina, Degeneration | 4 4 |
|__________________________________________________________________________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hyperplasia | |
__________________________________________|__________________________________________________________________________|
Page 85
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
_____________________________________________________________________________________________________________________
| 7| 7| 5| 7| 7| 6| 1| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 5| 7|
DAY ON TEST | 3| 2| 0| 3| 3| 5| 9| 6| 3| 3| 3| 3| 3| 3| 1| 2| 2| 3| 3| 0| 3| 3| 3| 9| 3|
| 0| 9| 8| 3| 3| 2| 1| 3| 3| 3| 3| 3| 1| 1| 5| 9| 9| 0| 1| 3| 1| 1| 1| 7| 2|
__________________________________________|__________________________________________________________________________|
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|
50 MG/KG | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|
__________________________________________|__________________________________________________________________________|
SPECIAL SENSES SYSTEM - cont | |
Inflammation, Chronic | 1 1 1 |
Pigmentation | 2 |
|__________________________________________________________________________|
Zymbal's Gland | |
_____________________________________________________________________________________________________________________|
URINARY SYSTEM | |
|__________________________________________________________________________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hydronephrosis | |
Nephropathy | 2 1 1 1 1 2 1 1 1 1 1 1 1 1 2 3 1|
|__________________________________________________________________________|
Urethra | |
Inflammation | |
|__________________________________________________________________________|
Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +|
Hemorrhage | |
Inflammation, Chronic | |
__________________________________________|__________________________________________________________________________
Page 86
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7| |
DAY ON TEST | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3| |
| 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Perforation | | 2 3.5|
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + A + + + + + + + + + + + + + +| 47 |
Inflammation, Chronic | | 1 2.0|
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + A + + + + + + + + + + + + + +| 48 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + A + + + + + + + + + + + + + +| 48 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + A + + + + + + + + + + + + + +| 48 |
Hyperplasia, Lymphoid | | 1 3.0|
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Basophilic Focus | X X | 6 2.0|
Clear Cell Focus | X X X 2 X | 12 2.0|
Degeneration, Cystic | | 1 2.0|
Eosinophilic Focus | X | 3 |
Hepatodiaphragmatic Nodule | 2 2 | 4 2.0|
Inflammation, Chronic | | 1 1.0|
Bile Duct, Hyperplasia | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2| 37 2.0|
Centrilobular, Necrosis | 2 2 | 2 2.0|
Hepatocyte, Vacuolization Cytoplasmic| 1 3 | 3 2.3|
Serosa, Inflammation | | 1 2.0|
|__________________________________________________________________________|____________|
Mesentery | + + + + + + + + + | 12 |
Accessory Spleen | 2 | 1 2.0|
Fat, Necrosis | 3 3 3 2 3 | 7 2.6|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 87
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7| |
DAY ON TEST | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3| |
| 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Oral Mucosa | + + | 4 |
Pharyngeal, Cyst | 4 4 | 2 4.0|
Pharyngeal, Inflammation | 2 3 | 2 2.5|
|__________________________________________________________________________|____________|
Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Atrophy, Focal | 1 | 1 1.0|
Atrophy | 2 4 1 2 2 1 1 2 2 2 2 3 1 4 2 2 | 32 1.9|
Cyst | | 1 2.0|
Inflammation, Chronic | | 1 2.0|
Acinus, Hyperplasia, Focal | 1 2 2 1 3 2| 12 1.8|
|__________________________________________________________________________|____________|
Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | | 1 2.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Edema | 1 4 | 2 2.5|
Erosion | | 1 1.0|
Ulcer | 2 3 | 2 2.5|
Epithelium, Hyperplasia | 1 2 | 3 1.3|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Edema | 2 | 2 2.0|
Erosion | | 1 2.0|
Ulcer | | 1 4.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Blood Vessel | + | 1 |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cardiomyopathy | 2 3 1 1 2 1 2 1 2 2 2 1 1 1 1 1 2 2 2 1 2 2| 45 1.7|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 88
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7| |
DAY ON TEST | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3| |
| 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Accessory Adrenal Cortical Nodule | 1 1 1 1 1 1 1 2 1 | 25 1.2|
Degeneration, Fatty | 1 | 2 1.5|
Hyperplasia, Focal | 2 1 | 5 1.2|
Hypertrophy, Focal | 2 2 1 3 2 2 | 8 2.0|
Necrosis | | 1 3.0|
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | | 2 2.0|
|__________________________________________________________________________|____________|
Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 2 2 1 1 2 1 1 1 2 1 2 1| 26 1.4|
|__________________________________________________________________________|____________|
Parathyroid Gland | + + + + + + + + + + + + + + + + + + M + + + + + +| 49 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Pars Distalis, Angiectasis | 2 2 3 2 3 2 | 15 2.2|
Pars Distalis, Cyst | 2 3 | 5 2.0|
Pars Distalis, Hyperplasia, Focal | 2 3 2 2 3 2| 13 2.3|
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Ultimobranchial Cyst | | 2 1.5|
C-Cell, Hyperplasia | 3 2 | 4 2.3|
Follicle, Cyst | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
|__________________________________________________________________________|____________|
Peritoneum | + + | 2 |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 89
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7| |
DAY ON TEST | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3| |
| 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
GENITAL SYSTEM - cont | | |
Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 1 2 | 3 1.7|
Inflammation, Chronic | 2 1 2 2 | 4 1.8|
|__________________________________________________________________________|____________|
Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Inflammation, Chronic | 1 2 2 2 3 3 3 2 2 2 2 2 2 2| 26 2.1|
|__________________________________________________________________________|____________|
Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Germinal Epithelium, Atrophy | | 1 3.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 3 4 2 | 9 2.7|
|__________________________________________________________________________|____________|
Lymph Node | + + + + + + + + + | 18 |
Mediastinal, Hyperplasia, Lymphoid | 2 2 2 2 2 2 2 2 | 15 2.0|
Pancreatic, Hyperplasia, Lymphoid | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | M M M M M M M M M M M M M M M M M M M M M M M M M| |
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia, Lymphoid | 2 2 1 2 | 9 2.1|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Fibrosis | 2 | 2 2.0|
Hematopoietic Cell Proliferation | 2 3 1 1 2 1 1 1 1 2 1 | 20 1.7|
Hemorrhage | 3 | 2 2.5|
Capsule, Fibrosis | 2 1 2 1 2 2 2 2 1 2 2 1 2| 30 1.8|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
_____________________________________________________________________________________________________________________| |
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 90
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7| |
DAY ON TEST | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3| |
| 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Cyst | 2 2 2 2 1 2 2 2 2 2 1 | 20 1.9|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperkeratosis | | 1 2.0|
Inflammation, Chronic | | 1 2.0|
Subcutaneous Tissue, Mineralization | 2| 1 2.0|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
|__________________________________________________________________________|____________|
Skeletal Muscle | + + + | 4 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Compression | 3 2 3 | 6 2.3|
|__________________________________________________________________________|____________|
Peripheral Nerve | + + | 6 |
|__________________________________________________________________________|____________|
Spinal Cord | + + | 6 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Congestion | 2 2 | 7 1.9|
Foreign Body | | 2 2.0|
Infiltration Cellular, Histiocyte | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1| 26 1.0|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 4 1 1 1| 30 1.2|
Inflammation, Suppurative | 3 | 1 3.0|
Necrosis | | 1 2.0|
Alveolar Epithelium, Hyperplasia | 1 3 | 6 2.2|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 91
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7| |
DAY ON TEST | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3| |
| 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
RESPIRATORY SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Foreign Body | 1 2 2 | 7 1.7|
Inflammation, Chronic | 2 2 1 2 2 2 3 2 | 15 1.9|
|__________________________________________________________________________|____________|
Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Foreign Body | | 1 2.0|
Necrosis | | 1 2.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 |
Cataract | 2 | 3 2.7|
Inflammation, Chronic | | 1 4.0|
Phthisis Bulbi | | 1 |
Anterior Chamber, Inflammation, Acute| | 1 2.0|
Retina, Degeneration | 3 | 3 3.7|
|__________________________________________________________________________|____________|
Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hyperplasia | 2 | 1 2.0|
Inflammation, Chronic | 1 | 4 1.0|
Pigmentation | 2 2 | 3 2.0|
|__________________________________________________________________________|____________|
Zymbal's Gland | + | 1 |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 |
Hydronephrosis | 2 3 | 2 2.5|
Nephropathy | 1 1 2 1 1 2 1 1 1 1 1 1 1 1 3 1 2| 34 1.3|
|__________________________________________________________________________|____________|
Urethra | + | 1 |
Inflammation | 3 | 1 3.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 92
NTP Experiment-Test: 91005-07 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 03/25/05
Route: GAVAGE Time: 10:12:13
__________________________________________________________________________________________________________________________________
| 6| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 5| 7| 4| 7| 6| 4| 7| 7| 5| 7| 7| |
DAY ON TEST | 8| 3| 5| 3| 3| 2| 2| 2| 3| 2| 6| 2| 2| 1| 7| 3| 6| 3| 9| 0| 3| 3| 0| 3| 3| |
| 7| 2| 8| 1| 1| 9| 9| 9| 1| 6| 6| 6| 9| 7| 6| 2| 5| 0| 2| 1| 2| 2| 4| 3| 3| |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O |
FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A |
50 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| |
_____________________________________________________________________________________________________________________|____________|
URINARY SYSTEM - cont | | |
Urinary Bladder | + + + + + + + + + + A + + + + + + + + + + + + + +| 49 |
Hemorrhage | 2 | 1 2.0|
Inflammation, Chronic | 3 | 1 3.0|
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 93
------------------------------------------------------------
---------- END OF REPORT ----------
------------------------------------------------------------