TDMS Study 99034-01 Pathology Tables
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
FINAL #1 MICE
Facility: Battelle Columbus Laboratory
Chemical CAS #: 71-43-2
Lock Date: 10/15/01
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
Page 1
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 8| 8| 9| 9| 9| 8| 8| 9| 8| 9| 9| 9| | |
| 0| 0| 0| 9| 9| 0| 0| 0| 9| 9| 0| 9| 0| 0| 0| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
0 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 | 10 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
Hyperplasia | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
Infiltration Cellular, Lymphoid | 1 | 1 1.0|
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 2
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 8| 8| 9| 9| 9| 8| 8| 9| 8| 9| 9| 9| | |
| 0| 0| 0| 9| 9| 0| 0| 0| 9| 9| 0| 9| 0| 0| 0| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
0 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Clitoral Gland | + | 1 |
Pigmentation | 3 | 1 3.0|
|__________________________________________________________________________|____________|
Ovary | + + + + I + + + + + + + + + + | 14 |
Cyst | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + | 15 |
Endometrium, Hyperplasia, Cystic | 1 1 1 1 1 1 1 1 1 1 1 1 | 12 1.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Lymph Node | M + + + + + + + + + + + + + M | 13 |
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + I + + + + + + + + + + + + | 14 |
Hyperplasia, Lymphoid | 1 | 1 1.0|
Infiltration Cellular, | | |
Polymorphonuclear | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 1 1 1 1 1 | 5 1.0|
Infiltration Cellular, Lymphocyte | 4 | 1 4.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 3
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 8| 8| 9| 9| 9| 8| 8| 9| 8| 9| 9| 9| | |
| 0| 0| 0| 9| 9| 0| 0| 0| 9| 9| 0| 9| 0| 0| 0| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
0 MG/KG | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
Inflammation, Granulomatous | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 2 | 2 1.5|
Alveolar Epithelium, Hyperplasia, | | |
Focal | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 1 1 1 1 | 5 1.0|
Infiltration Cellular, Lymphoid | 1 | 1 1.0|
Infiltration Cellular, Lymphocyte | 2 | 1 2.0|
Nephropathy | 1 1 1 | 3 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 4
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 9| 8| 8| 9| 9| 8| 9| 9| 9| 8| 9| 8| | |
| 0| 0| 0| 0| 9| 9| 0| 0| 9| 0| 0| 0| 9| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | A |
25 MG/KG | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 1 1 1 1 1 1 | 6 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Blood Vessel | + | 1 |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 5
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 9| 8| 8| 9| 9| 8| 9| 9| 9| 8| 9| 8| | |
| 0| 0| 0| 0| 9| 9| 0| 0| 9| 0| 0| 0| 9| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | A |
25 MG/KG | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Ovary | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + | 15 |
Endometrium, Hyperplasia, Cystic | 1 2 1 1 1 1 1 2 1 1 1 1 1 1 1 | 15 1.1|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Lymph Node | + + + + + + + M + M M + + + M | 11 |
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 |
Atrophy | 1 | 1 1.0|
Hyperplasia, Lymphoid | 2 1 1 1 | 4 1.3|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
Atrophy | 2 2 | 2 2.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 1 2 1 1 1 | 5 1.2|
Lymphoid Follicle, Atrophy | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
Hyperplasia, Atypical | 2 | 1 2.0|
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 6
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 9| 8| 8| 9| 9| 8| 9| 9| 9| 8| 9| 8| | |
| 0| 0| 0| 0| 9| 9| 0| 0| 9| 0| 0| 0| 9| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | A |
25 MG/KG | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
INTEGUMENTARY SYSTEM - cont | | |
Mammary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
Pigmentation | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 1 | 2 1.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 1 1 1 | 4 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 7
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 9| 8| 8| 9| 9| 9| 9| 9| 9| 8| 9| 9| 9| 8| | |
| 9| 0| 9| 9| 0| 0| 0| 0| 0| 0| 9| 0| 0| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
50 MG/KG | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 1 1 1 1 1 1 | 6 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 2 2 2 | 3 2.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 8
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 9| 8| 8| 9| 9| 9| 9| 9| 9| 8| 9| 9| 9| 8| | |
| 9| 0| 9| 9| 0| 0| 0| 0| 0| 0| 9| 0| 0| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
50 MG/KG | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Ovary | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + | 15 |
Inflammation, Suppurative | 1 | 1 1.0|
Endometrium, Hyperplasia, Cystic | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 | 15 1.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Lymph Node | + + M M M + + + + + + + + + M | 11 |
Mediastinal, Atrophy | 2 1 | 2 1.5|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 |
Atrophy | 1 1 | 2 1.0|
Hyperplasia, Lymphoid | 2 1 | 2 1.5|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
Atrophy | 1 1 1 | 3 1.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 1 1 | 2 1.0|
Lymphoid Follicle, Atrophy | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
Hyperplasia, Atypical | 4 | 1 4.0|
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
Inflammation, Granulomatous | 2 | 1 2.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 9
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 9| 8| 8| 9| 9| 9| 9| 9| 9| 8| 9| 9| 9| 8| | |
| 9| 0| 9| 9| 0| 0| 0| 0| 0| 0| 9| 0| 0| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
50 MG/KG | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
INTEGUMENTARY SYSTEM - cont | | |
Pigmentation | 1 1 1 1 1 1 1 1 | 8 1.0|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
Fibrosis | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 1 1 1 1 | 5 1.0|
Nephropathy | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 10
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 9| 9| 8| 8| 9| 9| 9| 8| 9| 8| 9| 8| | |
| 0| 0| 0| 0| 0| 9| 9| 0| 0| 0| 9| 0| 9| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
100 | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | L |
MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 2 | 1 2.0|
Inflammation, Chronic | 1 1 1 1 | 4 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
Erosion | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 11
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 9| 9| 8| 8| 9| 9| 9| 8| 9| 8| 9| 8| | |
| 0| 0| 0| 0| 0| 9| 9| 0| 0| 0| 9| 0| 9| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
100 | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | L |
MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Ovary | + + + + + + + + + + + + + + + | 15 |
Cyst | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + | 15 |
Inflammation, Suppurative | 1 | 1 1.0|
Endometrium, Hyperplasia, Cystic | 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 | 15 1.1|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Lymph Node | + + + + I + + + + M + + M + + | 12 |
Mediastinal, Atrophy | 1 2 1 1 | 4 1.3|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 |
Atrophy | 1 1 1 1 | 4 1.0|
Hyperplasia, Lymphoid | 1 1 1 3 1 | 5 1.4|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
Atrophy | 1 2 2 2 2 2 2 1 | 8 1.8|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 2 1 1 | 3 1.3|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
Hyperplasia, Atypical | 3 | 1 3.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 12
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 9| 9| 9| 9| 9| 8| 8| 9| 9| 9| 8| 9| 8| 9| 8| | |
| 0| 0| 0| 0| 0| 9| 9| 0| 0| 0| 9| 0| 9| 0| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
100 | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| | L |
MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
Inflammation, Suppurative | 1 | 1 1.0|
Pigmentation | 2 1 1 2 1 1 1 1 1 1 1 1 1 1 1 | 15 1.1|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
Fibrosis | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 1 1 1 1 1 1 1 | 8 1.0|
Nephropathy | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 13
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 9| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 8| 9| 9| | |
| 9| 0| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 9| 0| 0| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
200 | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | L |
MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 1 | 1 1.0|
Inflammation, Chronic | 1 1 1 1 1 1 1 1 1 1 1 | 11 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
Mineralization | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
Myocardium, Inflammation, Chronic | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 14
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 9| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 8| 9| 9| | |
| 9| 0| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 9| 0| 0| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
200 | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | L |
MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Ovary | + + + + + + + + + + + + + + + | 15 |
Angiectasis | 1 | 1 1.0|
Cyst | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Uterus | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 | 1 1.0|
Inflammation, Suppurative | 1 | 1 1.0|
Endometrium, Hyperplasia, Cystic | 1 1 1 1 1 1 1 1 1 1 1 1 2 1 | 14 1.1|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Lymph Node | + + + + + + + + + + M + + + M | 13 |
Lumbar, Hyperplasia, Lymphoid | 3 | 1 3.0|
Lumbar, Pigmentation | 2 | 1 2.0|
Mediastinal, Atrophy | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 |
Hyperplasia, Lymphoid | 1 1 1 | 3 1.0|
Infiltration Cellular, | | |
Polymorphonuclear | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
Atrophy | 2 1 1 1 2 1 | 6 1.3|
Hyperplasia, Lymphoid | 2 | 1 2.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 15
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 9| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 8| 9| 9| | |
| 9| 0| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 9| 0| 0| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
200 | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | L |
MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 1 1 1 2 1 1 1 | 7 1.1|
Lymphoid Follicle, Atrophy | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
Hyperplasia, Atypical | 2 | 1 2.0|
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | + + + + + + + + + + + + + + + | 15 |
Hyperplasia | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
Inflammation, Suppurative | 2 | 1 2.0|
Pigmentation | 1 1 1 1 1 2 2 1 1 1 1 1 1 1 1 | 15 1.1|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
Fibrosis | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 1 | 2 1.0|
Alveolar Epithelium, Hyperplasia, | | |
Focal | 1 2 1 | 3 1.3|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 16
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 9| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 8| 9| 9| | |
| 9| 0| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 9| 0| 0| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A |
200 | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | L |
MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 1 1 1 1 1 1 | 7 1.0|
Nephropathy | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 17
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 9| 8| 8| 8| 9| 8| 8| 8| 8| 8| 9| 8| 9| 8| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 1 1 1 | 3 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
Hyperplasia | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 18
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 9| 8| 8| 8| 9| 8| 8| 8| 8| 8| 9| 8| 9| 8| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + | 15 |
Unilateral, Hypospermia | 4 | 1 4.0|
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + | 15 |
Rete Testes, Inflammation, | | |
Granulomatous | 2 | 1 2.0|
Unilateral, Germinal Epithelium, | | |
Atrophy | 2 | 1 2.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
Pigmentation, Hemosiderin | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Lymph Node | M + M + + M + + + + + + + M + | 11 |
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 |
Hyperplasia, Lymphoid | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
Atrophy | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | M M M M M M M M M M M M M M M | |
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 19
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 9| 8| 8| 8| 9| 8| 8| 8| 8| 8| 9| 8| 9| 8| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
MUSCULOSKELETAL SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 1 | 2 1.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 | 1 1.0|
Nephropathy | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 20
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 8| 8| 9| 8| 9| 9| 8| 8| 9| 8| 8| 8| 8| 8| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
25 MG/KG | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 1 1 1 1 1 1 | 6 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
Myocardium, Inflammation, Chronic | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 21
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 8| 8| 9| 8| 9| 9| 8| 8| 9| 8| 8| 8| 8| 8| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
25 MG/KG | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
Pigmentation, Hemosiderin | 1 1 1 1 1 1 1 1 1 1 1 1 1 | 13 1.0|
|__________________________________________________________________________|____________|
Lymph Node | + + M + + + M M + + M + + + + | 11 |
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 |
Hyperplasia, Lymphoid | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
Atrophy | 2 2 | 2 2.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | M M M M M + M M M M M M M M M | 1 |
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
Pigmentation | 1 1 2 1 1 1 1 1 1 1 1 1 2 1 1 | 15 1.1|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 22
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 8| 8| 9| 8| 9| 9| 8| 8| 9| 8| 8| 8| 8| 8| 9| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
25 MG/KG | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| | L |
| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Nephropathy | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 23
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 9| 9| 8| 9| 8| 8| 8| 8| 8| 8| 9| 9| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
50 MG/KG | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
Mineralization | 1 | 1 1.0|
Glands, Cyst | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
Myocardium, Inflammation, Chronic | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 24
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 9| 9| 8| 9| 8| 8| 8| 8| 8| 8| 9| 9| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
50 MG/KG | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
Pigmentation, Hemosiderin | 1 1 1 1 1 1 1 1 1 1 1 1 2 | 13 1.1|
|__________________________________________________________________________|____________|
Lymph Node | + + + + + + + + + M M + + M + | 12 |
Mediastinal, Atrophy | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 15 |
Hyperplasia, Lymphoid | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + M + + + + + | 14 |
Atrophy | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | M M M M M M M M M M M M M M M | |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 25
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 9| 9| 8| 9| 8| 8| 8| 8| 8| 8| 9| 9| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
50 MG/KG | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| | L |
| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
INTEGUMENTARY SYSTEM - cont | | |
Skin | + + + + + + + + + + + + + + + | 15 |
Pigmentation | 2 2 1 2 1 2 1 2 1 1 2 2 2 2 1 | 15 1.6|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
Pigmentation, Hemosiderin | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 | 1 1.0|
Alveolar Epithelium, Hyperplasia, | | |
Focal | 1 | 1 1.0|
Bronchiole, Hyperplasia, Focal | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 26
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 8| 8| 8| 9| 9| 8| 8| 8| 9| 8| 9| 9| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
100 | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L |
MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 1 1 1 | 3 1.0|
|__________________________________________________________________________|____________|
Salivary Glands | + | 1 |
Submandibular Gland, Inflammation, | | |
Chronic | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
Inflammation, Acute | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
Glands, Cyst | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 27
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 8| 8| 8| 9| 9| 8| 8| 8| 9| 8| 9| 9| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
100 | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L |
MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + | 15 |
Germinal Epithelium, Atrophy | 2 | 1 2.0|
Germinal Epithelium, Syncytial | | |
Alteration | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
Atrophy | 1 2 1 1 1 1 1 1 1 1 | 10 1.1|
Pigmentation, Hemosiderin | 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 | 15 2.0|
|__________________________________________________________________________|____________|
Lymph Node | + + M + + + + M M M + + M + M | 9 |
Mediastinal, Atrophy | 2 1 1 1 1 2 | 6 1.3|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + M + + + + + + + + + + + | 14 |
Atrophy | 1 1 2 1 2 1 | 6 1.3|
Infiltration Cellular, | | |
Polymorphonuclear | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
Atrophy | 2 2 2 3 2 2 3 1 1 2 3 1 2 | 13 2.0|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 28
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 8| 8| 8| 9| 9| 8| 8| 8| 9| 8| 9| 9| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
100 | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L |
MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 1 1 | 2 1.0|
Lymphoid Follicle, Atrophy | 2 1 2 3 3 1 1 2 2 1 2 2 2 1 2 | 15 1.8|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
Atrophy | 4 4 3 2 3 3 2 | 7 3.0|
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | M M M M M M M M M M M M M M M | |
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
Pigmentation | 2 2 3 3 2 2 2 2 3 2 2 2 2 3 2 | 15 2.3|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 1 1 | 3 1.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
Harderian Gland | + | 1 |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 29
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 8| 8| 8| 9| 9| 8| 8| 8| 9| 8| 9| 9| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
100 | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | L |
MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | |
_____________________________________________________________________________________________________________________|____________|
URINARY SYSTEM - cont | | |
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 | 1 1.0|
Nephropathy | 1 1 | 2 1.0|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 30
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 1| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 1| 9| 9| 8| 8| 9| 8| 8| 9| 8| 8| 8| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
200 | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L |
MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ALIMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Intestine Large, Colon | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Rectum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Large, Cecum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Intestine Small, Ileum | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Liver | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Salivary Glands | + | 1 |
Submandibular Gland, Atrophy | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Stomach, Forestomach | + + + + + + + + + + + + + + + | 15 |
Erosion | 3 | 1 3.0|
Hyperplasia | 4 1 1 | 3 2.0|
|__________________________________________________________________________|____________|
Stomach, Glandular | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
CARDIOVASCULAR SYSTEM | | |
|__________________________________________________________________________|____________|
Blood Vessel | + | 1 |
|__________________________________________________________________________|____________|
Heart | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
ENDOCRINE SYSTEM | | |
|__________________________________________________________________________|____________|
Adrenal Cortex | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 31
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 1| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 1| 9| 9| 8| 8| 9| 8| 8| 9| 8| 8| 8| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
200 | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L |
MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
ENDOCRINE SYSTEM - cont | | |
|__________________________________________________________________________|____________|
Adrenal Medulla | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Pituitary Gland | + + + + + + + + + + + + + + + | 15 |
|__________________________________________________________________________|____________|
Thyroid Gland | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
GENERAL BODY SYSTEM | | |
None | | |
_____________________________________________________________________________________________________________________| |
GENITAL SYSTEM | | |
|__________________________________________________________________________|____________|
Epididymis | + + + + + + + + + + + + + + + | 15 |
Granuloma Sperm | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Testes | + + + + + + + + + + + + + + + | 15 |
Germinal Epithelium, Syncytial | | |
Alteration | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
HEMATOPOIETIC SYSTEM | | |
|__________________________________________________________________________|____________|
Bone Marrow | + + + + + + + + + + + + + + + | 15 |
Atrophy | 1 1 1 2 2 1 3 1 1 1 1 1 | 12 1.3|
Pigmentation, Hemosiderin | 3 3 3 3 3 3 3 3 1 3 3 3 3 3 | 14 2.9|
|__________________________________________________________________________|____________|
Lymph Node | M M + + + + M + M M + + M M M | 7 |
Mediastinal, Atrophy | 1 2 1 1 2 3 | 6 1.7|
|__________________________________________________________________________|____________|
Lymph Node, Mandibular | + + + + + M + + + + + + + + + | 14 |
Atrophy | 1 1 1 2 1 1 1 1 1 1 2 | 11 1.2|
Infiltration Cellular, | | |
Polymorphonuclear | 1 | 1 1.0|
|__________________________________________________________________________|____________|
Lymph Node, Mesenteric | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 32
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 1| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 1| 9| 9| 8| 8| 9| 8| 8| 9| 8| 8| 8| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
200 | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L |
MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
HEMATOPOIETIC SYSTEM - cont | | |
Atrophy | 2 2 2 2 1 3 1 2 2 1 1 1 2 | 13 1.7|
Infiltration Cellular, | | |
Polymorphonuclear | 2 | 1 2.0|
|__________________________________________________________________________|____________|
Spleen | + + + + + + + + + + + + + + + | 15 |
Hematopoietic Cell Proliferation | 1 2 2 1 1 1 1 1 | 8 1.3|
Lymphoid Follicle, Atrophy | 3 3 3 3 3 3 2 2 3 3 3 3 3 3 | 14 2.9|
|__________________________________________________________________________|____________|
Thymus | + + + + + + + + + + + + + + + | 15 |
Atrophy | 3 3 1 3 4 2 4 3 4 3 2 3 3 | 13 2.9|
Mineralization | 1 | 1 1.0|
_____________________________________________________________________________________________________________________| |
INTEGUMENTARY SYSTEM | | |
|__________________________________________________________________________|____________|
Mammary Gland | M M M M M M M M M M M M M M M | |
|__________________________________________________________________________|____________|
Skin | + + + + + + + + + + + + + + + | 15 |
Pigmentation | 3 2 3 2 2 2 3 3 3 3 3 2 2 3 | 14 2.6|
_____________________________________________________________________________________________________________________| |
MUSCULOSKELETAL SYSTEM | | |
|__________________________________________________________________________|____________|
Bone | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
NERVOUS SYSTEM | | |
|__________________________________________________________________________|____________|
Brain | + + + + + + + + + + + + + + + | 15 |
_____________________________________________________________________________________________________________________| |
RESPIRATORY SYSTEM | | |
|__________________________________________________________________________|____________|
Lung | + + + + + + + + + + + + + + + | 15 |
Inflammation, Chronic Active | 1 1 1 1 1 | 5 1.0|
_____________________________________________________________________________________________________________________| |
SPECIAL SENSES SYSTEM | | |
|__________________________________________________________________________|____________|
_____________________________________________________________________________________________________________________|____________|
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 33
NTP Experiment-Test: 99034-01 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:54:28
__________________________________________________________________________________________________________________________________
| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | |
DAY ON TEST | 1| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | |
| 1| 9| 9| 8| 8| 9| 8| 8| 9| 8| 8| 8| 8| 8| 8| | |
__________________________________________|__________________________________________________________________________| T (*) |
| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O |
P16(INK4A)/(+/-) (C57BL/6) MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T |
ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | A |
200 | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| | L |
MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | |
_____________________________________________________________________________________________________________________|____________|
SPECIAL SENSES SYSTEM - cont | | |
Eye | + | 1 |
_____________________________________________________________________________________________________________________| |
URINARY SYSTEM | | |
|__________________________________________________________________________|____________|
Kidney | + + + + + + + + + + + + + + + | 15 |
Casts Protein | 1 1 | 2 1.0|
Nephropathy | 1 1 2 1 | 4 1.3|
|__________________________________________________________________________|____________|
Urinary Bladder | + + + + + + + + + + + + + + + | 15 |
__________________________________________________________________________________________________________________________________
* : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade
+ : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as:
X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate
I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked
Page 34
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