NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
Facility: Southern Research Institute
Chemical CAS #: 1825-21-4
Lock Date: None
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include 001 VEHICLE CONTROL
Include 004 VEHICLE CONTROL
Include 007 40 MG/KG
Include 010 40 MG/KG
Include 013 20 MG/KG
Include 016 20 MG/KG
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE VEHICLE 20 MG/KG 40 MG/KG
CONTROL
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50
Early Deaths
Moribund Sacrifice 17 18 29
Natural Death 8 6 5
Accidently Killed 1
Dosing Accident 1
Survivors
Terminal Sacrifice 24 25 16
Animals Examined Microscopically 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (46) (23) (49)
Dilatation 1 (2%)
Liver (50) (50) (50)
Angiectasis 1 (2%) 1 (2%) 1 (2%)
Basophilic Focus 2 (4%) 1 (2%)
Cytologic Alterations 1 (2%) 34 (68%) 39 (78%)
Eosinophilic Focus 5 (10%) 1 (2%) 1 (2%)
Eosinophilic Focus, Multiple 3 (6%)
Hematopoietic Cell Proliferation 9 (18%) 6 (12%) 9 (18%)
Inflammation, Subacute 1 (2%) 28 (56%) 32 (64%)
Mixed Cell Focus 2 (4%) 2 (4%)
Mixed Cell Focus, Multiple 5 (10%)
Necrosis 1 (2%) 2 (4%) 2 (4%)
Vacuolization Cytoplasmic 1 (2%)
Biliary Tract, Dilatation 1 (2%)
Biliary Tract, Hyperplasia 1 (2%) 16 (32%) 30 (60%)
Kupffer Cell, Pigmentation 37 (74%) 48 (96%)
Mesentery (25) (18) (24)
Hemorrhage 1 (6%)
Inflammation, Suppurative 8 (32%) 7 (39%) 8 (33%)
Fat, Necrosis 14 (56%) 8 (44%) 8 (33%)
Pancreas (50) (28) (50)
Acinar Cell, Atrophy 4 (8%) 3 (11%) 2 (4%)
Duct, Dilatation 1 (2%) 1 (4%) 2 (4%)
Salivary Glands (50) (25) (49)
Atrophy 1 (2%)
Foreign Body 1 (2%)
Stomach, Forestomach (50) (45) (50)
Cyst Epithelial Inclusion 1 (2%)
Foreign Body 1 (2%)
Hyperplasia 12 (24%) 7 (16%) 6 (12%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE VEHICLE 20 MG/KG 40 MG/KG
CONTROL
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (50) (25) (50)
Inflammation, Subacute 1 (2%)
Mineralization 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland (50) (50) (49)
Capsule, Spindle Cell, Hyperplasia 1 (2%)
Adrenal Gland, Cortex (50) (50) (49)
Accessory Adrenal Cortical Nodule 2 (4%) 1 (2%)
Hypertrophy, Focal 1 (2%)
Necrosis 1 (2%)
Parathyroid Gland (47) (25) (48)
Cyst 1 (4%)
Pituitary Gland (47) (49) (50)
Pars Distalis, Cyst 1 (2%)
Pars Distalis, Hyperplasia 12 (26%) 8 (16%) 4 (8%)
Thyroid Gland (50) (50) (50)
Inflammation, Subacute 1 (2%)
Follicular Cell, Hyperplasia 5 (10%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (1) (1)
Duct, Cyst 1 (100%) 1 (100%)
Ovary (45) (36) (50)
Abscess 12 (27%) 14 (39%) 18 (36%)
Cyst 3 (7%) 8 (22%) 5 (10%)
Hemorrhage 3 (8%)
Uterus (46) (43) (50)
Dilatation 9 (20%) 4 (8%)
Hyperplasia, Cystic 42 (91%) 36 (84%) 46 (92%)
Inflammation, Suppurative 5 (11%) 6 (14%) 10 (20%)
Mucosa, Cyst 2 (4%) 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE VEHICLE 20 MG/KG 40 MG/KG
CONTROL
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (25) (50)
Myeloid Cell, Hypercellularity 17 (34%) 11 (44%) 21 (42%)
Lymph Node (50) (50) (50)
Iliac, Hyperplasia, Lymphoid 1 (2%) 1 (2%) 2 (4%)
Iliac, Inflammation, Suppurative 1 (2%)
Mediastinal, Hyperplasia, Lymphoid 1 (2%)
Mediastinal, Inflammation, Suppurative 2 (4%) 1 (2%)
Renal, Hyperplasia, Lymphoid 1 (2%) 1 (2%) 4 (8%)
Renal, Inflammation, Suppurative 1 (2%)
Lymph Node, Mandibular (49) (47) (49)
Hyperplasia, Lymphoid 1 (2%)
Lymph Node, Mesenteric (50) (49) (50)
Angiectasis 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 2 (4%)
Spleen (50) (50) (50)
Hematopoietic Cell Proliferation 21 (42%) 18 (36%) 28 (56%)
Hyperplasia, Lymphoid 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (49) (23) (50)
Hyperplasia 1 (2%)
Skin (50) (26) (50)
Inflammation 1 (4%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (25) (50)
Hypertrophy 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (26) (50)
Hemorrhage 1 (2%)
Inflammation, Subacute 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (27) (50)
Abscess 1 (2%)
Foreign Body 1 (2%)
Infiltration Cellular, Histiocyte 1 (2%) 2 (7%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE VEHICLE 20 MG/KG 40 MG/KG
CONTROL
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Inflammation, Suppurative 1 (4%)
Alveolar Epithelium, Hyperplasia 1 (2%) 2 (7%)
Mediastinum, Inflammation, Suppurative 5 (10%) 5 (19%) 3 (6%)
Nose (50) (50) (50)
Foreign Body 7 (14%) 15 (30%) 19 (38%)
Fungus 1 (2%)
Inflammation, Suppurative 5 (10%) 13 (26%) 21 (42%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Harderian Gland (6) (2) (4)
Hyperplasia 2 (33%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (25) (50)
Amyloid Deposition 2 (4%)
Hydronephrosis 1 (2%) 2 (8%)
Nephropathy 12 (24%) 3 (12%) 8 (16%)
Cortex, Cyst 1 (2%)
Glomerulus, Inflammation 2 (8%)
Transitional Epithelium, Hyperplasia 1 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE VEHICLE 20 MG/KG 40 MG/KG
CONTROL
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50
Early Deaths
Natural Death 2 2
Moribund Sacrifice 17 20 22
Accidently Killed 1
Dosing Accident 1
Survivors
Terminal Sacrifice 30 27 27
Natural Death 1
Animals Examined Microscopically 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Intestine Large, Colon (50) (23) (50)
Inflammation, Suppurative 1 (2%)
Intestine Large, Rectum (50) (23) (50)
Inflammation, Suppurative 1 (2%)
Intestine Small, Ileum (50) (22) (49)
Hyperplasia 1 (5%)
Liver (50) (50) (50)
Angiectasis 1 (2%) 3 (6%)
Basophilic Focus 5 (10%) 1 (2%) 1 (2%)
Basophilic Focus, Multiple 1 (2%)
Clear Cell Focus 2 (4%) 1 (2%) 2 (4%)
Clear Cell Focus, Multiple 3 (6%) 3 (6%)
Cytologic Alterations 50 (100%) 50 (100%)
Eosinophilic Focus 8 (16%) 1 (2%)
Eosinophilic Focus, Multiple 2 (4%)
Fibrosis 1 (2%)
Hematopoietic Cell Proliferation 1 (2%) 2 (4%)
Inflammation, Subacute 49 (98%) 49 (98%)
Mixed Cell Focus 7 (14%) 4 (8%) 5 (10%)
Mixed Cell Focus, Multiple 2 (4%) 11 (22%) 22 (44%)
Necrosis 2 (4%) 1 (2%)
Biliary Tract, Dilatation 1 (2%) 2 (4%)
Biliary Tract, Fibrosis 1 (2%)
Biliary Tract, Hyperplasia 47 (94%) 48 (96%)
Kupffer Cell, Pigmentation 1 (2%) 50 (100%) 50 (100%)
Mesentery (2) (9) (4)
Hemorrhage 2 (22%)
Fat, Necrosis 2 (100%) 5 (56%) 2 (50%)
Pancreas (50) (23) (50)
Acinar Cell, Atrophy 5 (10%) 4 (17%) 3 (6%)
Duct, Dilatation 3 (6%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE VEHICLE 20 MG/KG 40 MG/KG
CONTROL
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Stomach, Forestomach (50) (50) (50)
Hyperplasia 19 (38%) 23 (46%) 24 (48%)
Stomach, Glandular (50) (48) (49)
Hyperplasia 1 (2%)
Tooth (36) (10) (24)
Dysplasia 35 (97%) 10 (100%) 23 (96%)
Foreign Body 1 (3%)
Inflammation, Chronic 1 (4%)
Inflammation, Subacute 1 (3%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (50) (23) (50)
Inflammation, Subacute 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland (50) (50) (49)
Capsule, Spindle Cell, Hyperplasia 4 (8%) 2 (4%) 3 (6%)
Adrenal Gland, Cortex (50) (50) (49)
Accessory Adrenal Cortical Nodule 1 (2%) 1 (2%)
Hypertrophy, Focal 9 (18%) 4 (8%) 7 (14%)
Adrenal Gland, Medulla (50) (50) (48)
Hyperplasia 13 (26%) 29 (60%)
Hypertrophy 3 (6%) 36 (75%)
Parathyroid Gland (49) (22) (50)
Cyst 2 (4%)
Pituitary Gland (44) (50) (47)
Pars Distalis, Cyst 3 (7%) 1 (2%) 4 (9%)
Pars Distalis, Hyperplasia 1 (2%) 1 (2%)
Thyroid Gland (50) (49) (50)
Follicular Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (50) (24) (50)
Granuloma Sperm 1 (2%)
Preputial Gland (22) (32) (26)
Inflammation, Subacute 3 (14%) 5 (16%) 2 (8%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE VEHICLE 20 MG/KG 40 MG/KG
CONTROL
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Duct, Cyst 20 (91%) 26 (81%) 26 (100%)
Seminal Vesicle (50) (24) (50)
Inflammation, Subacute 1 (4%)
Testes (50) (23) (50)
Atrophy 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (23) (50)
Myeloid Cell, Hypercellularity 2 (4%)
Lymph Node (50) (50) (50)
Inguinal, Hyperplasia, Lymphoid 1 (2%)
Mediastinal, Hyperplasia, Lymphoid 1 (2%)
Lymph Node, Mesenteric (50) (49) (50)
Angiectasis 1 (2%)
Congestion 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 2 (4%) 1 (2%)
Thrombosis 1 (2%)
Spleen (50) (50) (50)
Hematopoietic Cell Proliferation 7 (14%) 8 (16%) 10 (20%)
Hemorrhage 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (50) (29) (50)
Inflammation, Suppurative 1 (3%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (24) (50)
Hypertrophy 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (23) (50)
Hemorrhage 1 (2%)
Necrosis 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 05037-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC PENTACHLOROANISOLE Date: 06/23/97
Route: GAVAGE Time: 12:43:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE VEHICLE 20 MG/KG 40 MG/KG
CONTROL
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (32) (50)
Foreign Body 1 (3%)
Hemorrhage 1 (3%) 1 (2%)
Infiltration Cellular, Histiocyte 7 (14%) 3 (9%) 5 (10%)
Thrombosis 1 (2%)
Alveolar Epithelium, Hyperplasia 7 (14%) 4 (13%) 2 (4%)
Nose (50) (50) (50)
Exudate, Serous 1 (2%)
Foreign Body 2 (4%) 14 (28%) 10 (20%)
Inflammation, Suppurative 5 (10%) 12 (24%) 10 (20%)
Mucosa, Atrophy 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (25) (50)
Inflammation, Subacute 1 (4%)
Nephropathy 21 (42%) 12 (48%) 17 (34%)
Cortex, Cyst 4 (8%) 3 (12%) 4 (8%)
Renal Tubule, Hyperplasia 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
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