TDMS Study 05085-02 Pathology Tables
NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 Facility: Battelle Columbus Laboratory Chemical CAS #: 7785-87-7 Lock Date: None Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Page 1 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 4| 4| 7| 7| 7| 7| 6| 4| 7| 7| 7| 2| 7| 7| 7| 4| 2| 7| 3| 7| 3| DAY ON TEST | 2| 2| 2| 5| 5| 5| 2| 2| 2| 0| 2| 5| 2| 2| 3| 7| 3| 3| 3| 5| 7| 3| 2| 3| 9| | 9| 9| 9| 2| 2| 3| 9| 9| 9| 8| 0| 3| 9| 9| 0| 7| 0| 0| 0| 3| 6| 0| 3| 0| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Cytomegaly, Multifocal | 2 | Eosinophilic Focus | X X X | Inflammation, Chronic | | Vacuolization Cytoplasmic | 3 2 2| Hepatocyte, Atrophy | | Kupffer Cell, Hyperplasia | | __________________________________________________________________________| Mesentery | + + + | Fat, Inflammation, Chronic Active | 3 3 2 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Lymphocyte | | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | | Ulcer | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration | | Inflammation, Chronic | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | Hypertrophy | 2 1 | Vacuolization Cytoplasmic | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X | _____________________________________________________________________________________________________________________| Page 2 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 4| 4| 7| 7| 7| 7| 6| 4| 7| 7| 7| 2| 7| 7| 7| 4| 2| 7| 3| 7| 3| DAY ON TEST | 2| 2| 2| 5| 5| 5| 2| 2| 2| 0| 2| 5| 2| 2| 3| 7| 3| 3| 3| 5| 7| 3| 2| 3| 9| | 9| 9| 9| 2| 2| 3| 9| 9| 9| 8| 0| 3| 9| 9| 0| 7| 0| 0| 0| 3| 6| 0| 3| 0| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Hyperplasia | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + M + + + + + + + + + + + + M + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | M + + + + + + + + + + + M + + + + + + + + + + + +| Pars Distalis, Cyst | X X | Pars Distalis, Hyperplasia | 2 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + | Inflammation, Chronic Active | | Follicle, Cyst | X X X X X | Follicle, Dilatation | | Follicular Cell, Hyperplasia, Diffuse| 1 | Follicular Cell, Hyperplasia, Focal | 4 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | 3 3 | Inflammation, Chronic Active | | Bilateral, Atrophy | | Bilateral, Follicle, Cyst | | Follicle, Cyst | X X X X X | Periovarian Tissue, Cyst | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | 2 | Endometrium, Hyperplasia, Cystic, | | Glandular | 3 3 4 3 3 2 3 4 3 4 2 4 3 3 3 3 3 2 2 3 2 4 2| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Femoral, Atrophy | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + M + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Hematopoietic Cell Proliferation | 3 | __________________________________________________________________________| Thymus | + + + + + M + + + + + + + + + + M + + + + + + + +| Depletion Lymphoid | 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 3 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 4| 4| 7| 7| 7| 7| 6| 4| 7| 7| 7| 2| 7| 7| 7| 4| 2| 7| 3| 7| 3| DAY ON TEST | 2| 2| 2| 5| 5| 5| 2| 2| 2| 0| 2| 5| 2| 2| 3| 7| 3| 3| 3| 5| 7| 3| 2| 3| 9| | 9| 9| 9| 2| 2| 3| 9| 9| 9| 8| 0| 3| 9| 9| 0| 7| 0| 0| 0| 3| 6| 0| 3| 0| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | Alopecia | X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | Back, Inflammation, Chronic Active | 3 | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + M M + + + + + + + + + + + + + M M + + + +| __________________________________________________________________________| Spinal Cord | + +| Lumbar, Degeneration | 2 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Perivascular, Inflammation, Chronic | | Active | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Harderian Gland | | Hyperplasia | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Nephropathy | 2 2 1 1 1 2 2 2 2 1 1 1 1 2 1 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 4 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 4| 2| 4| 7| 4| 2| 7| 7| 7| 2| 7| 6| 7| 2| 2| DAY ON TEST | 3| 7| 3| 3| 3| 3| 3| 3| 3| 3| 5| 7| 5| 3| 5| 7| 3| 2| 3| 7| 3| 0| 3| 7| 7| | 0| 7| 0| 1| 1| 1| 1| 1| 1| 1| 3| 7| 2| 1| 3| 6| 2| 0| 2| 6| 2| 7| 2| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| ANIMAL ID | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 0PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + M + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | | Cytomegaly, Multifocal | | Eosinophilic Focus | X X | Inflammation, Chronic | | Vacuolization Cytoplasmic | 2 | Hepatocyte, Atrophy | | Kupffer Cell, Hyperplasia | | __________________________________________________________________________| Mesentery | + + + | Fat, Inflammation, Chronic Active | 3 4 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | 3 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Lymphocyte | 2 | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 2 | Ulcer | 2 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration | 2 | Inflammation, Chronic | 2 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 3 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 | Hypertrophy | | Vacuolization Cytoplasmic | 3 2 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | | _____________________________________________________________________________________________________________________| Page 5 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 4| 2| 4| 7| 4| 2| 7| 7| 7| 2| 7| 6| 7| 2| 2| DAY ON TEST | 3| 7| 3| 3| 3| 3| 3| 3| 3| 3| 5| 7| 5| 3| 5| 7| 3| 2| 3| 7| 3| 0| 3| 7| 7| | 0| 7| 0| 1| 1| 1| 1| 1| 1| 1| 3| 7| 2| 1| 3| 6| 2| 0| 2| 6| 2| 7| 2| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| ANIMAL ID | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 0PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Hyperplasia | 3 | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + M + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + M + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Cyst | | Pars Distalis, Hyperplasia | 2 2 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Follicle, Cyst | X X X X | Follicle, Dilatation | | Follicular Cell, Hyperplasia, Diffuse| | Follicular Cell, Hyperplasia, Focal | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + M + + + + + + + + + + + + + +| Atrophy | | Inflammation, Chronic Active | 4 | Bilateral, Atrophy | | Bilateral, Follicle, Cyst | | Follicle, Cyst | X X X X X X | Periovarian Tissue, Cyst | X X| __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | | Endometrium, Hyperplasia, Cystic, | | Glandular | 4 2 4 4 3 2 4 3 4 3 2 3 4 3 2 4 3 3 2 3 2 4 2 2| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Femoral, Atrophy | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + M + + + + + + +| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Hematopoietic Cell Proliferation | 3 | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + M + + + + + + + +| Depletion Lymphoid | 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + M + + + + + + + + + + M + + + + + + M + + + +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 6 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 4| 2| 4| 7| 4| 2| 7| 7| 7| 2| 7| 6| 7| 2| 2| DAY ON TEST | 3| 7| 3| 3| 3| 3| 3| 3| 3| 3| 5| 7| 5| 3| 5| 7| 3| 2| 3| 7| 3| 0| 3| 7| 7| | 0| 7| 0| 1| 1| 1| 1| 1| 1| 1| 3| 7| 2| 1| 3| 6| 2| 0| 2| 6| 2| 7| 2| 6| 6| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| ANIMAL ID | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 0PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | Alopecia | X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Back, Inflammation, Chronic Active | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + M M + + + M + + + + + + + + +| __________________________________________________________________________| Spinal Cord | | Lumbar, Degeneration | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 1 | Perivascular, Inflammation, Chronic | | Active | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Harderian Gland | + | Hyperplasia | 2 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Nephropathy | 1 1 1 1 1 2 2 1 4 1 1 1 1 1 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 7 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 5| 7| 2| 7| 2| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 2| | | DAY ON TEST | 3| 3| 9| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 3| 7| | | | 2| 2| 6| 7| 2| 6| 2| 6| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | A | 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 70 | Basophilic Focus | | 1 | Cytomegaly, Multifocal | | 1 2.0| Eosinophilic Focus | X X X | 8 | Inflammation, Chronic | 3 | 1 3.0| Vacuolization Cytoplasmic | 3 1 | 6 2.2| Hepatocyte, Atrophy | 2 | 1 2.0| Kupffer Cell, Hyperplasia | 4 | 1 4.0| __________________________________________________________________________|____________| Mesentery | + + | 8 | Fat, Inflammation, Chronic Active | 3 | 6 3.0| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 70 | Acinus, Atrophy | | 1 3.0| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | Infiltration Cellular, Lymphocyte | | 1 2.0| __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia, Focal, Squamous | | 1 2.0| Ulcer | 2 | 2 2.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 70 | Degeneration | | 1 2.0| Inflammation, Chronic | | 1 2.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic Active | | 1 3.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 8 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 5| 7| 2| 7| 2| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 2| | | DAY ON TEST | 3| 3| 9| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 3| 7| | | | 2| 2| 6| 7| 2| 6| 2| 6| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | A | 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Hyperplasia | 2 2 | 4 2.0| Hypertrophy | | 2 1.5| Vacuolization Cytoplasmic | | 2 2.5| __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + | 70 | Cyst | | 1 | Hyperplasia | | 1 3.0| __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + M + + + + + + + | 66 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + | 67 | Pars Distalis, Cyst | X | 3 | Pars Distalis, Hyperplasia | 1 2 2 | 6 1.8| __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Chronic Active | 1 | 1 1.0| Follicle, Cyst | X X X X X X X X X | 18 | Follicle, Dilatation | 1 | 1 1.0| Follicular Cell, Hyperplasia, Diffuse| | 1 1.0| Follicular Cell, Hyperplasia, Focal | 2 | 3 2.3| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | __________________________________________________________________________|____________| Tissue NOS | + | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + | 69 | Atrophy | | 2 3.0| Inflammation, Chronic Active | | 1 4.0| Bilateral, Atrophy | 3 | 1 3.0| Bilateral, Follicle, Cyst | X | 1 | Follicle, Cyst | X X X X X X X X | 19 | Periovarian Tissue, Cyst | X | 3 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + | 70 | Hemorrhage | | 1 2.0| Endometrium, Hyperplasia, Cystic, | | | Glandular | 4 4 3 3 3 1 4 3 3 4 4 3 4 4 2 4 4 3 2 | 66 3.1| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | Femoral, Atrophy | 2 | 1 2.0| __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + | 67 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 9 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 5| 7| 2| 7| 2| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| 7| 2| | | DAY ON TEST | 3| 3| 9| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 3| 7| | | | 2| 2| 6| 7| 2| 6| 2| 6| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 6| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| | A | 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + M + + + + + + + M + + M + + + | 64 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 70 | Depletion Lymphoid | 2 | 1 2.0| Hematopoietic Cell Proliferation | | 2 3.0| __________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + M + + + + | 66 | Depletion Lymphoid | 2 | 3 2.3| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + M + + + + + + M M M + + + + + + + + | 62 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | Alopecia | | 4 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | Back, Inflammation, Chronic Active | | 1 3.0| _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + M + M + + + + + + + M + + + + | 60 | __________________________________________________________________________|____________| Spinal Cord | | 2 | Lumbar, Degeneration | | 1 2.0| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic Active | 2 | 2 1.5| Perivascular, Inflammation, Chronic | | | Active | 2 | 1 2.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | 1 | __________________________________________________________________________|____________| Harderian Gland | + + + + + | 6 | Hyperplasia | 2 2 | 3 2.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | Nephropathy | 2 2 3 2 1 2 1 1 1 1 1 1 | 41 1.4| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 10 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 4| 7| 2| 6| 2| 7| 7| 4| 7| 7| 4| 7| 7| 7| DAY ON TEST | 5| 2| 2| 7| 2| 2| 2| 2| 2| 2| 2| 5| 3| 7| 7| 7| 3| 3| 5| 3| 3| 5| 3| 2| 3| | 3| 9| 9| 6| 9| 9| 9| 9| 9| 9| 9| 2| 0| 6| 9| 6| 0| 0| 3| 0| 0| 2| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X X X | Clear Cell Focus | X X X| Eosinophilic Focus | X X X X X | Infarct | | Inflammation, Acute, Focal | 2 | Inflammation, Chronic Active | | Vacuolization Cytoplasmic | 2 | __________________________________________________________________________| Mesentery | + | Fat, Inflammation, Chronic Active | 3 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | 3 2| Duct, Cyst | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 2 | Inflammation, Chronic Active | 2 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | Hypertrophy | 1 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + +| Pars Distalis, Cyst | X X X | _____________________________________________________________________________________________________________________| Page 11 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 4| 7| 2| 6| 2| 7| 7| 4| 7| 7| 4| 7| 7| 7| DAY ON TEST | 5| 2| 2| 7| 2| 2| 2| 2| 2| 2| 2| 5| 3| 7| 7| 7| 3| 3| 5| 3| 3| 5| 3| 2| 3| | 3| 9| 9| 6| 9| 9| 9| 9| 9| 9| 9| 2| 0| 6| 9| 6| 0| 0| 3| 0| 0| 2| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Pars Distalis, Hyperplasia | 2 2 3 3 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 1 | Follicle, Cyst | X X X X | Follicle, Dilatation | 1 1 | Follicular Cell, Hyperplasia, Diffuse| 1 | Follicular Cell, Hyperplasia, Focal | 2 4 1 1| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | + | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + +| Mineralization | | Follicle, Cyst | X X X X X| Periovarian Tissue, Cyst | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + +| Endometrium, Hyperplasia, Cystic, | | Glandular | 4 3 3 4 3 4 3 2 3 3 3 3 3 3 2 3 2 3| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Histiocyte | 3 | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + M + + + + + + +| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 3 3 | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + M + + + + M + + + M + + +| Hyperplasia, Cystic | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + +| Abscess | | Acanthosis | | Alopecia | | Ulcer | 4 3 | Subcutaneous Tissue, Inflammation, | | Chronic Active | 3 3 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 12 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 4| 7| 2| 6| 2| 7| 7| 4| 7| 7| 4| 7| 7| 7| DAY ON TEST | 5| 2| 2| 7| 2| 2| 2| 2| 2| 2| 2| 5| 3| 7| 7| 7| 3| 3| 5| 3| 3| 5| 3| 2| 3| | 3| 9| 9| 6| 9| 9| 9| 9| 9| 9| 9| 2| 0| 6| 9| 6| 0| 0| 3| 0| 0| 2| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + +| Hydrocephalus | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | 2 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + +| Nephropathy | 2 1 1 2 2 1 1 1 1 1 1 1 1 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 13 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 2| 7| 2| 7| 7| 7| 4| 7| 4| 7| 7| 2| 4| 7| 7| 2| 7| 7| 2| 7| 7| 7| DAY ON TEST | 5| 3| 3| 7| 3| 7| 3| 3| 3| 5| 3| 5| 3| 3| 7| 5| 3| 1| 7| 3| 3| 7| 3| 3| 3| | 2| 0| 0| 7| 1| 7| 1| 1| 1| 2| 1| 3| 1| 1| 6| 3| 1| 2| 6| 1| 2| 7| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| ANIMAL ID | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 1500 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + M + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X X | Clear Cell Focus | X | Eosinophilic Focus | X | Infarct | 2 | Inflammation, Acute, Focal | 2 | Inflammation, Chronic Active | 1 | Vacuolization Cytoplasmic | | __________________________________________________________________________| Mesentery | + | Fat, Inflammation, Chronic Active | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + +| Acinus, Atrophy | 2 | Duct, Cyst | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 2 1 | Inflammation, Chronic Active | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 | Hypertrophy | 2 2 2 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + +| Hyperplasia | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + M + + +| __________________________________________________________________________| Pituitary Gland | M + M M + + + + + + + + + + + +| Pars Distalis, Cyst | | _____________________________________________________________________________________________________________________| Page 14 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 2| 7| 2| 7| 7| 7| 4| 7| 4| 7| 7| 2| 4| 7| 7| 2| 7| 7| 2| 7| 7| 7| DAY ON TEST | 5| 3| 3| 7| 3| 7| 3| 3| 3| 5| 3| 5| 3| 3| 7| 5| 3| 1| 7| 3| 3| 7| 3| 3| 3| | 2| 0| 0| 7| 1| 7| 1| 1| 1| 2| 1| 3| 1| 1| 6| 3| 1| 2| 6| 1| 2| 7| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| ANIMAL ID | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 1500 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Pars Distalis, Hyperplasia | 3 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 1 | Follicle, Cyst | X X X X X X| Follicle, Dilatation | | Follicular Cell, Hyperplasia, Diffuse| | Follicular Cell, Hyperplasia, Focal | 1 2 1 2 1 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + +| Mineralization | | Follicle, Cyst | X X X X X X X | Periovarian Tissue, Cyst | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + +| Endometrium, Hyperplasia, Cystic, | | Glandular | 3 3 3 3 3 3 4 3 3 3 3 3 4 3 3 2 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + +| Infiltration Cellular, Histiocyte | 3 | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + M + + + + + + +| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 3 | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + M| Depletion Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + M + + + + + + + + + + +| Hyperplasia, Cystic | 4 | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + +| Abscess | 4 | Acanthosis | 3 | Alopecia | X X | Ulcer | 4 | Subcutaneous Tissue, Inflammation, | | Chronic Active | 3 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 15 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 2| 7| 2| 7| 7| 7| 4| 7| 4| 7| 7| 2| 4| 7| 7| 2| 7| 7| 2| 7| 7| 7| DAY ON TEST | 5| 3| 3| 7| 3| 7| 3| 3| 3| 5| 3| 5| 3| 3| 7| 5| 3| 1| 7| 3| 3| 7| 3| 3| 3| | 2| 0| 0| 7| 1| 7| 1| 1| 1| 2| 1| 3| 1| 1| 6| 3| 1| 2| 6| 1| 2| 7| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| ANIMAL ID | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 1500 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + +| Hydrocephalus | 2 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + +| Nephropathy | 2 2 1 1 1 1 1 1 1 1 1 1| __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 16 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 2| 7| 7| 7| 7| 4| 7| 2| 7| | | DAY ON TEST | 3| 0| 3| 3| 5| 3| 3| 3| 3| 3| 3| 7| 3| 3| 3| 3| 5| 3| 7| 3| | | | 2| 1| 2| 2| 3| 2| 2| 3| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 7| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | A | 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + | 60 | Basophilic Focus | X X | 7 | Clear Cell Focus | X | 5 | Eosinophilic Focus | X X | 8 | Infarct | | 1 2.0| Inflammation, Acute, Focal | | 2 2.0| Inflammation, Chronic Active | | 1 1.0| Vacuolization Cytoplasmic | 2 3 | 3 2.3| __________________________________________________________________________|____________| Mesentery | + | 3 | Fat, Inflammation, Chronic Active | 2 | 2 2.5| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + | 50 | Acinus, Atrophy | 4 | 4 2.8| Duct, Cyst | X | 2 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + | 50 | Hyperplasia, Focal, Squamous | | 3 1.7| Inflammation, Chronic Active | | 1 2.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + | 50 | Hyperplasia | 2 1 2 2 | 7 1.9| Hypertrophy | | 4 1.8| __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 17 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 2| 7| 7| 7| 7| 4| 7| 2| 7| | | DAY ON TEST | 3| 0| 3| 3| 5| 3| 3| 3| 3| 3| 3| 7| 3| 3| 3| 3| 5| 3| 7| 3| | | | 2| 1| 2| 2| 3| 2| 2| 3| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 7| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | A | 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Hyperplasia | 2 | 2 2.0| __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + M + + + + + + + + | 46 | Pars Distalis, Cyst | | 3 | Pars Distalis, Hyperplasia | 2 2 | 7 2.4| __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + | 50 | Inflammation, Chronic Active | | 2 1.0| Follicle, Cyst | X X | 12 | Follicle, Dilatation | 1 1 1 | 5 1.0| Follicular Cell, Hyperplasia, Diffuse| | 1 1.0| Follicular Cell, Hyperplasia, Focal | 1 1 1 2 1 | 15 1.5| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | __________________________________________________________________________|____________| Tissue NOS | | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + | 51 | Mineralization | 2 | 1 2.0| Follicle, Cyst | X X X X X X X | 19 | Periovarian Tissue, Cyst | X | 2 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + | 52 | Endometrium, Hyperplasia, Cystic, | | | Glandular | 2 4 3 3 3 3 3 3 3 3 3 3 3 4 3 | 49 3.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + | 50 | Infiltration Cellular, Histiocyte | | 2 3.0| __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + | 50 | Hematopoietic Cell Proliferation | | 3 3.0| __________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + | 49 | Depletion Lymphoid | 2 3 | 3 2.3| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + M + + + | 45 | Hyperplasia, Cystic | | 1 4.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 18 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 2| 7| 7| 7| 7| 4| 7| 2| 7| | | DAY ON TEST | 3| 0| 3| 3| 5| 3| 3| 3| 3| 3| 3| 7| 3| 3| 3| 3| 5| 3| 7| 3| | | | 2| 1| 2| 2| 3| 2| 2| 3| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 7| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| | A | 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + | 50 | Abscess | | 1 4.0| Acanthosis | | 1 3.0| Alopecia | | 2 | Ulcer | 4 | 4 3.8| Subcutaneous Tissue, Inflammation, | | | Chronic Active | 3 | 4 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + | 50 | Hydrocephalus | | 1 2.0| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + | 50 | Alveolar Epithelium, Hyperplasia | | 1 2.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | + | 3 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + | 50 | Nephropathy | 1 1 1 1 1 1 1 1 2 1 1 | 36 1.2| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 19 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 2| 7| 7| 4| 6| 7| 7| 7| 4| 4| 4| 4| 6| 7| 4| 7| 7| 7| 7| 2| 7| DAY ON TEST | 7| 2| 2| 2| 7| 2| 2| 5| 9| 2| 2| 2| 5| 5| 5| 5| 9| 3| 0| 2| 0| 3| 3| 7| 3| | 7| 9| 9| 9| 6| 9| 9| 2| 3| 9| 3| 9| 2| 2| 3| 3| 6| 0| 9| 5| 2| 0| 0| 7| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | | Clear Cell Focus | | Eosinophilic Focus | X | Hepatodiaphragmatic Nodule | X | Infarct | 2 | Inflammation, Chronic Active | | Vacuolization Cytoplasmic | 3 | __________________________________________________________________________| Mesentery | + | Fat, Inflammation, Chronic Active | 2 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + +| Acinus, Atrophy | | Duct, Cyst | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 2 2 | Ulcer | 2 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + +| Mineralization | 3 | Ulcer | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + +| Corticomedullary Junction, | | Vacuolization Cytoplasmic | | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + +| Hyperplasia | 2 1 | Hypertrophy | 3 | Mineralization | 2 | Vacuolization Cytoplasmic | | _____________________________________________________________________________________________________________________| Page 20 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 2| 7| 7| 4| 6| 7| 7| 7| 4| 4| 4| 4| 6| 7| 4| 7| 7| 7| 7| 2| 7| DAY ON TEST | 7| 2| 2| 2| 7| 2| 2| 5| 9| 2| 2| 2| 5| 5| 5| 5| 9| 3| 0| 2| 0| 3| 3| 7| 3| | 7| 9| 9| 9| 6| 9| 9| 2| 3| 9| 3| 9| 2| 2| 3| 3| 6| 0| 9| 5| 2| 0| 0| 7| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + M + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + +| Pars Distalis, Cyst | | Pars Distalis, Hyperplasia | 3 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Lymphocyte | | Inflammation, Chronic Active | | Inflammation, Granulomatous | 1 | Follicle, Cyst | X X X | Follicle, Dilatation | 1 1 1 2 | Follicular Cell, Hyperplasia, Focal | 1 2 1 1 3 1 1 3 2 1| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + +| Thrombosis | X | Follicle, Cyst | X X X X X X X X | Periovarian Tissue, Cyst | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + +| Endometrium, Hyperplasia, Cystic, | | Glandular | 4 3 3 3 3 2 3 3 3 3 3 4 3 3 3 2 4 4 3| Endometrium, Thrombosis | X| Lymphatic, Cyst | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + +| Mediastinal, Infiltration Cellular, | | Histiocyte | 3 | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + +| Depletion Lymphoid | 3 | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + M + + + + + + + + + + +| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Hematopoietic Cell Proliferation | 4 3 3| Necrosis | 4 | __________________________________________________________________________| Thymus | + + + + + M + + + + + M + + + + +| Depletion Lymphoid | 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 21 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 2| 7| 7| 4| 6| 7| 7| 7| 4| 4| 4| 4| 6| 7| 4| 7| 7| 7| 7| 2| 7| DAY ON TEST | 7| 2| 2| 2| 7| 2| 2| 5| 9| 2| 2| 2| 5| 5| 5| 5| 9| 3| 0| 2| 0| 3| 3| 7| 3| | 7| 9| 9| 9| 6| 9| 9| 2| 3| 9| 3| 9| 2| 2| 3| 3| 6| 0| 9| 5| 2| 0| 0| 7| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + M M| Hyperplasia, Cystic | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + +| Alopecia | X| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + +| Cerebellum, Cyst Epithelial Inclusion| | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + +| Nephropathy | 1 1 1 1 1 1 1 4 1 4 1 2 1 2 1| __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + M + + + + +| _____________________________________________________________________________________________________________________| Page 22 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 2| 2| 0| 7| 6| 2| 7| 7| 7| 7| 7| 7| 7| 7| 4| 2| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 7| 7| 1| 3| 0| 7| 3| 3| 3| 3| 3| 3| 3| 3| 5| 7| 3| 3| 3| 3| 3| | 0| 0| 0| 0| 6| 7| 9| 1| 7| 6| 1| 1| 1| 1| 1| 1| 1| 2| 3| 7| 2| 2| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| ANIMAL ID | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 5000 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Clear Cell Focus | X | Eosinophilic Focus | X | Hepatodiaphragmatic Nodule | | Infarct | | Inflammation, Chronic Active | 2 | Vacuolization Cytoplasmic | 3 1 1 | __________________________________________________________________________| Mesentery | | Fat, Inflammation, Chronic Active | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | 2 | Duct, Cyst | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 2 | Ulcer | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + +| Mineralization | | Ulcer | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + +| Corticomedullary Junction, | | Vacuolization Cytoplasmic | 3 | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 1 2 1 | Hypertrophy | 2 2 | Mineralization | | Vacuolization Cytoplasmic | 3 | _____________________________________________________________________________________________________________________| Page 23 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 2| 2| 0| 7| 6| 2| 7| 7| 7| 7| 7| 7| 7| 7| 4| 2| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 7| 7| 1| 3| 0| 7| 3| 3| 3| 3| 3| 3| 3| 3| 5| 7| 3| 3| 3| 3| 3| | 0| 0| 0| 0| 6| 7| 9| 1| 7| 6| 1| 1| 1| 1| 1| 1| 1| 2| 3| 7| 2| 2| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| ANIMAL ID | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 5000 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + M + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + +| Pars Distalis, Cyst | X | Pars Distalis, Hyperplasia | 2 2 1 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + M + + + + + + + + +| Infiltration Cellular, Lymphocyte | 2 | Inflammation, Chronic Active | 1 | Inflammation, Granulomatous | | Follicle, Cyst | X X | Follicle, Dilatation | 3 2 1 | Follicular Cell, Hyperplasia, Focal | 1 1 3 1 2 3 1 1 1 1 1 1 1| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + +| Thrombosis | | Follicle, Cyst | X X X X X X | Periovarian Tissue, Cyst | X X| __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + +| Endometrium, Hyperplasia, Cystic, | | Glandular | 4 3 3 3 4 3 4 3 3 3 2 3 3 4 3 4 3 4 3 4| Endometrium, Thrombosis | | Lymphatic, Cyst | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + +| Mediastinal, Infiltration Cellular, | | Histiocyte | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + M + + + +| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Hematopoietic Cell Proliferation | | Necrosis | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 24 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 2| 2| 0| 7| 6| 2| 7| 7| 7| 7| 7| 7| 7| 7| 4| 2| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 7| 7| 1| 3| 0| 7| 3| 3| 3| 3| 3| 3| 3| 3| 5| 7| 3| 3| 3| 3| 3| | 0| 0| 0| 0| 6| 7| 9| 1| 7| 6| 1| 1| 1| 1| 1| 1| 1| 2| 3| 7| 2| 2| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| ANIMAL ID | 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 5000 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Mammary Gland | M + + + M + M + + + + + + + M + + +| Hyperplasia, Cystic | 3 | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + +| Alopecia | X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + +| Cerebellum, Cyst Epithelial Inclusion| | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | 1| __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + +| Nephropathy | 1 1 1 1 1 1 1 1 1 2 1 1 1 1| __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 25 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 6| 7| 2| 7| 1| 4| 5| 7| 7| 7| 7| 7| 4| 7| 7| 2| 2| 7| 4| 4| | | DAY ON TEST | 1| 3| 7| 3| 0| 5| 5| 3| 0| 3| 3| 3| 5| 3| 3| 7| 7| 3| 5| 8| | | | 0| 2| 7| 2| 7| 3| 4| 3| 4| 3| 3| 3| 3| 3| 3| 7| 7| 3| 2| 8| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | A | 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Gallbladder | A + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + | 59 | Basophilic Focus | X X | 3 | Clear Cell Focus | X | 2 | Eosinophilic Focus | X | 3 | Hepatodiaphragmatic Nodule | X | 2 | Infarct | | 1 2.0| Inflammation, Chronic Active | | 1 2.0| Vacuolization Cytoplasmic | 3 1 2 | 7 2.0| __________________________________________________________________________|____________| Mesentery | | 1 | Fat, Inflammation, Chronic Active | | 1 2.0| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + | 50 | Acinus, Atrophy | 3 | 2 2.5| Duct, Cyst | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + | 52 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + | 50 | Hyperplasia, Focal, Squamous | 2 | 4 2.0| Ulcer | | 1 2.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + | 51 | Mineralization | | 1 3.0| Ulcer | 2 3 | 2 2.5| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + | 50 | Corticomedullary Junction, | | | Vacuolization Cytoplasmic | | 1 3.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 26 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 6| 7| 2| 7| 1| 4| 5| 7| 7| 7| 7| 7| 4| 7| 7| 2| 2| 7| 4| 4| | | DAY ON TEST | 1| 3| 7| 3| 0| 5| 5| 3| 0| 3| 3| 3| 5| 3| 3| 7| 7| 3| 5| 8| | | | 0| 2| 7| 2| 7| 3| 4| 3| 4| 3| 3| 3| 3| 3| 3| 7| 7| 3| 2| 8| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | A | 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + | 48 | Hyperplasia | | 6 1.5| Hypertrophy | 2 1 2 | 6 2.0| Mineralization | | 1 2.0| Vacuolization Cytoplasmic | | 1 3.0| __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + | 48 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + | 50 | Pars Distalis, Cyst | | 1 | Pars Distalis, Hyperplasia | | 4 2.0| __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 52 | Infiltration Cellular, Lymphocyte | | 1 2.0| Inflammation, Chronic Active | 2 1 | 3 1.3| Inflammation, Granulomatous | | 1 1.0| Follicle, Cyst | X X X X | 9 | Follicle, Dilatation | 1 1 1 1 1 | 12 1.3| Follicular Cell, Hyperplasia, Focal | 1 1 3 1 | 27 1.5| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + | 53 | Thrombosis | | 1 | Follicle, Cyst | X X X X | 18 | Periovarian Tissue, Cyst | | 3 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + | 54 | Endometrium, Hyperplasia, Cystic, | | | Glandular | 2 3 4 2 3 3 4 3 4 4 2 | 50 3.2| Endometrium, Thrombosis | | 1 | Lymphatic, Cyst | X | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + M + + + | 49 | Mediastinal, Infiltration Cellular, | | | Histiocyte | | 1 3.0| __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + M + + + | 47 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 27 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 6| 7| 2| 7| 1| 4| 5| 7| 7| 7| 7| 7| 4| 7| 7| 2| 2| 7| 4| 4| | | DAY ON TEST | 1| 3| 7| 3| 0| 5| 5| 3| 0| 3| 3| 3| 5| 3| 3| 7| 7| 3| 5| 8| | | | 0| 2| 7| 2| 7| 3| 4| 3| 4| 3| 3| 3| 3| 3| 3| 7| 7| 3| 2| 8| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| | A | 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Depletion Lymphoid | | 1 3.0| __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + M M + + + | 44 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + | 50 | Depletion Lymphoid | 3 | 1 3.0| Hematopoietic Cell Proliferation | 4 | 4 3.5| Necrosis | | 1 4.0| __________________________________________________________________________|____________| Thymus | M + + + + + + + + + + + + + | 47 | Depletion Lymphoid | 2 2 | 3 2.3| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M + + M + M + + M + + + + + | 39 | Hyperplasia, Cystic | | 1 3.0| __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + | 50 | Alopecia | | 3 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + | 50 | Cerebellum, Cyst Epithelial Inclusion| X | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + | 50 | Alveolar Epithelium, Hyperplasia | | 2 1.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + | 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + | 50 | Nephropathy | 1 1 1 1 | 33 1.3| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + | 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 28 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 7| 7| 7| 2| 7| 7| 2| 7| 4| 2| 2| 4| 7| 4| 7| 7| 2| 4| 4| DAY ON TEST | 2| 2| 2| 2| 2| 4| 2| 2| 2| 7| 2| 3| 7| 3| 5| 7| 7| 8| 3| 5| 3| 3| 7| 5| 5| | 9| 9| 9| 9| 9| 7| 9| 9| 9| 7| 9| 0| 7| 0| 2| 7| 7| 8| 0| 3| 0| 0| 6| 3| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + M + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Congestion | | Eosinophilic Focus | X X X | Inflammation, Chronic Active | 2 | Mixed Cell Focus | X X | Vacuolization Cytoplasmic | 1 4 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 4 | Duct, Cyst | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Erosion, Focal | 4 4 | Hyperplasia, Focal, Squamous | 2 2 1 3 | Inflammation, Chronic Active | 3 | Ulcer | 3 2 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | Hypertrophy | 2 1 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | __________________________________________________________________________| Parathyroid Gland | + + + + + + + M + + + + + + + + M + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Cyst | | Pars Distalis, Hyperplasia | 1 3 3 2 2 | _____________________________________________________________________________________________________________________| Page 29 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 7| 7| 7| 2| 7| 7| 2| 7| 4| 2| 2| 4| 7| 4| 7| 7| 2| 4| 4| DAY ON TEST | 2| 2| 2| 2| 2| 4| 2| 2| 2| 7| 2| 3| 7| 3| 5| 7| 7| 8| 3| 5| 3| 3| 7| 5| 5| | 9| 9| 9| 9| 9| 7| 9| 9| 9| 7| 9| 0| 7| 0| 2| 7| 7| 8| 0| 3| 0| 0| 6| 3| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | X X | Follicle, Dilatation | 1 1 2 1 1 1 1 1 1 1 1 1| Follicular Cell, Hyperplasia, Focal | 1 1 4 1 2 2 4 1 1 3 2 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosis | | Bilateral, Follicle, Cyst | | Follicle, Cyst | X X X| Periovarian Tissue, Cyst | X X X X X | Periovarian Tissue, Inflammation, | | Chronic Active | 4 | Rete Ovarii, Cyst | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Cervix, Inflammation, Chronic Active | | Endometrium, Angiectasis | | Endometrium, Hyperplasia, Cystic, | | Glandular | 4 4 4 4 4 2 4 3 3 3 3 3 2 3 3 3 3 3 4 3 3 3 3 3 2| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Femoral, Atrophy | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + M + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + M + M| Inflammation, Chronic Active | 4 | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 3 | Inflammation, Chronic Active | 4 | Thrombosis | | __________________________________________________________________________| Thymus | + + + + + A + + + + + + + + + + + + + + M + + + +| Depletion Lymphoid | | Inflammation, Chronic Active | 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + M M M + + M + M + M M + + + + + + + M + + + +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Alopecia | X | Subcutaneous Tissue, Inflammation, | | Chronic Active | | _____________________________________________________________________________________________________________________| Page 30 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 4| 7| 7| 7| 2| 7| 7| 2| 7| 4| 2| 2| 4| 7| 4| 7| 7| 2| 4| 4| DAY ON TEST | 2| 2| 2| 2| 2| 4| 2| 2| 2| 7| 2| 3| 7| 3| 5| 7| 7| 8| 3| 5| 3| 3| 7| 5| 5| | 9| 9| 9| 9| 9| 7| 9| 9| 9| 7| 9| 0| 7| 0| 2| 7| 7| 8| 0| 3| 0| 0| 6| 3| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + M + + + + + + + + + M + + + M M| Compression | | Hemorrhage | | Hydrocephalus | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 3 | Alveolar Epithelium, Hyperplasia | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | Cornea, Inflammation, Chronic Active | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Nephropathy | 1 1 1 1 1 2 1 1 1 1 2 1 | Pelvis, Inflammation, Chronic Active | 3 | Renal Tubule, Cytomegaly | 2 | Renal Tubule, Regeneration | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 31 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 6| 7| 4| 2| 7| 7| 2| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 4| 7| DAY ON TEST | 3| 7| 3| 3| 3| 3| 3| 3| 5| 7| 3| 3| 7| 3| 3| 5| 5| 3| 7| 3| 3| 3| 3| 5| 3| | 0| 6| 0| 0| 1| 1| 1| 1| 2| 6| 1| 1| 7| 1| 1| 3| 2| 1| 6| 2| 2| 2| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| ANIMAL ID | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 15000 | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X X | Congestion | | Eosinophilic Focus | X | Inflammation, Chronic Active | | Mixed Cell Focus | X | Vacuolization Cytoplasmic | 1 1 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Duct, Cyst | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Erosion, Focal | | Hyperplasia, Focal, Squamous | 3 2 3 3 2 3 | Inflammation, Chronic Active | 1 1 | Ulcer | 3 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 1 2 2 | Hypertrophy | 2 2 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + M + + + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + M M + + + + + + + + + + + + + + + + + + + +| Pars Distalis, Cyst | | Pars Distalis, Hyperplasia | 2 | _____________________________________________________________________________________________________________________| Page 32 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 6| 7| 4| 2| 7| 7| 2| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 4| 7| DAY ON TEST | 3| 7| 3| 3| 3| 3| 3| 3| 5| 7| 3| 3| 7| 3| 3| 5| 5| 3| 7| 3| 3| 3| 3| 5| 3| | 0| 6| 0| 0| 1| 1| 1| 1| 2| 6| 1| 1| 7| 1| 1| 3| 2| 1| 6| 2| 2| 2| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| ANIMAL ID | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 15000 | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | X X X | Follicle, Dilatation | 2 1 1 1 1 1 2 1 1 1 1 1 1 1 1 | Follicular Cell, Hyperplasia, Focal | 1 1 4 1 1 2 2 2 1 2 2 1 4 4 2| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosis | 4 | Bilateral, Follicle, Cyst | X | Follicle, Cyst | X X X X | Periovarian Tissue, Cyst | X X | Periovarian Tissue, Inflammation, | | Chronic Active | | Rete Ovarii, Cyst | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Cervix, Inflammation, Chronic Active | | Endometrium, Angiectasis | | Endometrium, Hyperplasia, Cystic, | | Glandular | 4 3 3 4 4 3 3 3 2 3 3 3 3 4 3 3 3 3 4 3 3 4 2 3| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Femoral, Atrophy | | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + M + + + + + + + M + + + + + + + + + + + + +| Inflammation, Chronic Active | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | | Inflammation, Chronic Active | | Thrombosis | X | __________________________________________________________________________| Thymus | + M + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Inflammation, Chronic Active | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + M M M + + + + + + + + + + + + + + M + + M + +| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Alopecia | X | Subcutaneous Tissue, Inflammation, | | Chronic Active | | _____________________________________________________________________________________________________________________| Page 33 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 2| 7| 7| 7| 7| 6| 7| 4| 2| 7| 7| 2| 7| 7| 4| 4| 7| 2| 7| 7| 7| 7| 4| 7| DAY ON TEST | 3| 7| 3| 3| 3| 3| 3| 3| 5| 7| 3| 3| 7| 3| 3| 5| 5| 3| 7| 3| 3| 3| 3| 5| 3| | 0| 6| 0| 0| 1| 1| 1| 1| 2| 6| 1| 1| 7| 1| 1| 3| 2| 1| 6| 2| 2| 2| 2| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| ANIMAL ID | 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 15000 | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + M + + + + + + M + + + M + + + + + M + + + + + +| Compression | | Hemorrhage | | Hydrocephalus | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 2 | Alveolar Epithelium, Hyperplasia | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | Cornea, Inflammation, Chronic Active | 3 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Nephropathy | 1 1 1 1 1 1 1 1 2 1 1 1| Pelvis, Inflammation, Chronic Active | | Renal Tubule, Cytomegaly | | Renal Tubule, Regeneration | 1 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 34 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 2| 7| 4| 7| 6| 7| 7| 7| 6| 7| 7| 7| 7| 7| 4| | | DAY ON TEST | 3| 3| 3| 3| 7| 7| 3| 5| 3| 8| 3| 0| 3| 9| 3| 3| 3| 2| 3| 7| | | | 2| 2| 2| 3| 6| 7| 3| 2| 3| 9| 3| 9| 3| 6| 3| 3| 3| 6| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | A | 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + M + + | 67 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + | 69 | Basophilic Focus | X X | 5 | Congestion | 2 | 1 2.0| Eosinophilic Focus | | 4 | Inflammation, Chronic Active | | 1 2.0| Mixed Cell Focus | | 3 | Vacuolization Cytoplasmic | | 4 1.8| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Chronic Active | | 1 4.0| Duct, Cyst | | 1 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + M + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + | 68 | Erosion, Focal | | 2 4.0| Hyperplasia, Focal, Squamous | 2 | 11 2.4| Inflammation, Chronic Active | 1 | 4 1.5| Ulcer | | 3 2.7| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + | 67 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia | 2 | 5 1.8| Hypertrophy | 1 | 5 1.6| __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 35 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 2| 7| 4| 7| 6| 7| 7| 7| 6| 7| 7| 7| 7| 7| 4| | | DAY ON TEST | 3| 3| 3| 3| 7| 7| 3| 5| 3| 8| 3| 0| 3| 9| 3| 3| 3| 2| 3| 7| | | | 2| 2| 2| 3| 6| 7| 3| 2| 3| 9| 3| 9| 3| 6| 3| 3| 3| 6| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | A | 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Hyperplasia | | 1 2.0| __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + M + + + + + + + | 65 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + | 67 | Pars Distalis, Cyst | X | 1 | Pars Distalis, Hyperplasia | 1 2 2 1 | 10 1.9| __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 70 | Follicle, Cyst | X X | 7 | Follicle, Dilatation | 1 1 1 1 2 1 1 1 1 | 36 1.1| Follicular Cell, Hyperplasia, Focal | 1 4 2 2 1 1 4 2 3 2 2 2 4 1 3 1 | 43 2.1| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + | 69 | Fibrosis | | 1 4.0| Bilateral, Follicle, Cyst | | 1 | Follicle, Cyst | X X X X X X X | 14 | Periovarian Tissue, Cyst | X X | 9 | Periovarian Tissue, Inflammation, | | | Chronic Active | | 1 4.0| Rete Ovarii, Cyst | | 1 | __________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + | 69 | Cervix, Inflammation, Chronic Active | 3 | 1 3.0| Endometrium, Angiectasis | 3 | 1 3.0| Endometrium, Hyperplasia, Cystic, | | | Glandular | 4 3 3 2 3 3 3 3 3 3 2 3 4 4 3 3 2 | 66 3.1| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + | 69 | Femoral, Atrophy | 3 | 1 3.0| __________________________________________________________________________|____________| Lymph Node | + + + + + + + + M + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + M + + + + + + + + + | 62 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + M + + + M + + + + + + | 59 | Inflammation, Chronic Active | | 1 4.0| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + | 69 | Hematopoietic Cell Proliferation | 4 | 2 3.5| Inflammation, Chronic Active | | 1 4.0| Thrombosis | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 36 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 2| 7| 4| 7| 6| 7| 7| 7| 6| 7| 7| 7| 7| 7| 4| | | DAY ON TEST | 3| 3| 3| 3| 7| 7| 3| 5| 3| 8| 3| 0| 3| 9| 3| 3| 3| 2| 3| 7| | | | 2| 2| 2| 3| 6| 7| 3| 2| 3| 9| 3| 9| 3| 6| 3| 3| 3| 6| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE FEMALE | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| | A | 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Thymus | + + + + M + + + + + + + + M + + + + M | 63 | Depletion Lymphoid | 3 3 2 | 3 2.7| Inflammation, Chronic Active | | 1 2.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M + + + + + + + + + + + + M M + + + + | 53 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + | 69 | Alopecia | X | 3 | Subcutaneous Tissue, Inflammation, | | | Chronic Active | 4 | 1 4.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + M + + + + + + + + + + + + | 60 | Compression | X | 1 | Hemorrhage | 3 | 1 3.0| Hydrocephalus | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Chronic Active | | 2 2.5| Alveolar Epithelium, Hyperplasia | 2 | 1 2.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + | 67 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 1 | Cornea, Inflammation, Chronic Active | | 1 3.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + | 69 | Nephropathy | 1 1 3 1 1 1 2 | 31 1.2| Pelvis, Inflammation, Chronic Active | | 1 3.0| Renal Tubule, Cytomegaly | | 1 2.0| Renal Tubule, Regeneration | | 1 1.0| __________________________________________________________________________|____________| Urinary Bladder | + + + + M + + + + + + + + + + + + + + | 68 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 37 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 4| 7| 7| 7| 2| 7| 7| 7| 4| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| DAY ON TEST | 5| 2| 2| 5| 2| 2| 2| 7| 2| 2| 2| 5| 5| 2| 3| 3| 3| 3| 3| 3| 0| 3| 5| 3| 3| | 3| 9| 9| 2| 9| 9| 9| 6| 9| 9| 9| 2| 2| 9| 0| 0| 0| 0| 0| 0| 9| 0| 2| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + M + + + + + + + + + + + + M M + + M + + + + + +| Artery, Inflammation, Chronic Active | 3| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + M + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + M + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Clear Cell Focus | X | Eosinophilic Focus | | Hypertrophy | 2 | Infarct | 4| Necrosis | 2 2| Vacuolization Cytoplasmic | 3 3 1 1 1 3 2 2 2 2| Bile Duct, Hyperplasia | 2 | Centrilobular, Vacuolization | | Cytoplasmic | | __________________________________________________________________________| Mesentery | + + | Fat, Inflammation, Chronic Active | 2 3 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | 3 1 | Duct, Cyst | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 2 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Spindle Cell, Vacuolization | | Cytoplasmic | 3 | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Accessory Adrenal Cortical Nodule | X | Atrophy | | Hyperplasia | 1 | _____________________________________________________________________________________________________________________| Page 38 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 4| 7| 7| 7| 2| 7| 7| 7| 4| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| DAY ON TEST | 5| 2| 2| 5| 2| 2| 2| 7| 2| 2| 2| 5| 5| 2| 3| 3| 3| 3| 3| 3| 0| 3| 5| 3| 3| | 3| 9| 9| 2| 9| 9| 9| 6| 9| 9| 9| 2| 2| 9| 0| 0| 0| 0| 0| 0| 9| 0| 2| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Hypertrophy | 2 2 3 2 2 1 3 2 2 2 2 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 2 | __________________________________________________________________________| Parathyroid Gland | + + M + + + + + + + + + + + + + + + + M + + + + +| __________________________________________________________________________| Pituitary Gland | + + + M + + + + + + + M + + + + + M + + + + + + +| Pars Distalis, Cyst | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | X | Follicle, Dilatation | 1 1 | Follicular Cell, Hyperplasia, Focal | 1 1 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 4 | Duct, Dilatation | 3 3 3 3 3 3 3 3 3 3 3 3 4 3| __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Seminiferous Tubule, Atrophy | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + M + + M + + + + + + M + + + + + + + + + + +| Hematopoietic Cell Proliferation | | Infiltration Cellular, Plasma Cell | 2 | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | | Necrosis | 3 | __________________________________________________________________________| Thymus | + M + + M + + + M + + M + + + + + + + + M + + + +| Depletion Lymphoid | 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Alopecia | X | _____________________________________________________________________________________________________________________| Page 39 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 4| 7| 7| 7| 2| 7| 7| 7| 4| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| DAY ON TEST | 5| 2| 2| 5| 2| 2| 2| 7| 2| 2| 2| 5| 5| 2| 3| 3| 3| 3| 3| 3| 0| 3| 5| 3| 3| | 3| 9| 9| 2| 9| 9| 9| 6| 9| 9| 9| 2| 2| 9| 0| 0| 0| 0| 0| 0| 9| 0| 2| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | Ulcer | | Subcutaneous Tissue, Inflammation, | | Chronic Active | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | M + + M + + + + + + + + M + + + + + + + + + + + +| __________________________________________________________________________| Peripheral Nerve | | Sciatic, Demyelination | | __________________________________________________________________________| Spinal Cord | | Demyelination | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Mucosa, Inflammation, Acute | 2 | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + + + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Hydronephrosis | 4 | Nephropathy | 3 1 3 2 3 2 2 2 2 3 3 2 2 2 2 2 2 2| Renal Tubule, Regeneration | 2 1 1 1 2 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 40 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 7| 7| 4| 4| 2| 7| 7| 4| 7| 4| 2| 7| 7| 2| 7| 7| 7| 6| 2| 2| DAY ON TEST | 3| 3| 3| 5| 3| 3| 3| 5| 5| 7| 3| 3| 6| 3| 5| 7| 3| 3| 7| 3| 3| 3| 8| 7| 7| | 1| 1| 1| 3| 1| 1| 1| 3| 2| 6| 1| 1| 6| 1| 3| 6| 2| 2| 7| 2| 2| 2| 7| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 0PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | I + + + + A + + + + + + + + + + + + + + + M + + +| Artery, Inflammation, Chronic Active | | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + A + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + M| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | | Clear Cell Focus | | Eosinophilic Focus | X | Hypertrophy | | Infarct | 4 | Necrosis | | Vacuolization Cytoplasmic | 2 2 3 2 2 2 3 2 2 2 | Bile Duct, Hyperplasia | | Centrilobular, Vacuolization | | Cytoplasmic | 3 3 3 3| __________________________________________________________________________| Mesentery | | Fat, Inflammation, Chronic Active | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | | Duct, Cyst | X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 2 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + A + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Spindle Cell, Vacuolization | | Cytoplasmic | | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Accessory Adrenal Cortical Nodule | | Atrophy | | Hyperplasia | 3 2 | _____________________________________________________________________________________________________________________| Page 41 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 7| 7| 4| 4| 2| 7| 7| 4| 7| 4| 2| 7| 7| 2| 7| 7| 7| 6| 2| 2| DAY ON TEST | 3| 3| 3| 5| 3| 3| 3| 5| 5| 7| 3| 3| 6| 3| 5| 7| 3| 3| 7| 3| 3| 3| 8| 7| 7| | 1| 1| 1| 3| 1| 1| 1| 3| 2| 6| 1| 1| 6| 1| 3| 6| 2| 2| 7| 2| 2| 2| 7| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 0PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Hypertrophy | 2 2 2 2 2 1 2 2 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | __________________________________________________________________________| Parathyroid Gland | + + + + + + + M + + + M + M + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + M + + + + + + + + + + + + + + +| Pars Distalis, Cyst | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | | Follicle, Dilatation | | Follicular Cell, Hyperplasia, Focal | 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Preputial Gland | + + + + + + + + + + | Inflammation, Chronic Active | 4 3 2 | Duct, Dilatation | 4 3 3 3 2 4 4 3 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Seminiferous Tubule, Atrophy | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + M| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + M| Depletion Lymphoid | 2 | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + A + M + + + M + + + M M M| Hematopoietic Cell Proliferation | | Infiltration Cellular, Plasma Cell | | __________________________________________________________________________| Spleen | + + + + + + + + + M + + + + + + + + + + + + + + +| Depletion Lymphoid | | Necrosis | | __________________________________________________________________________| Thymus | + + + + + M + + M + + + + + + + + + M + + + + + +| Depletion Lymphoid | 2 2 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Alopecia | | _____________________________________________________________________________________________________________________| Page 42 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 7| 7| 4| 4| 2| 7| 7| 4| 7| 4| 2| 7| 7| 2| 7| 7| 7| 6| 2| 2| DAY ON TEST | 3| 3| 3| 5| 3| 3| 3| 5| 5| 7| 3| 3| 6| 3| 5| 7| 3| 3| 7| 3| 3| 3| 8| 7| 7| | 1| 1| 1| 3| 1| 1| 1| 3| 2| 6| 1| 1| 6| 1| 3| 6| 2| 2| 7| 2| 2| 2| 7| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 0PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | Ulcer | | Subcutaneous Tissue, Inflammation, | | Chronic Active | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + M M + + + + + + + + + + + + + + M +| __________________________________________________________________________| Peripheral Nerve | + | Sciatic, Demyelination | 2 | __________________________________________________________________________| Spinal Cord | + | Demyelination | 3 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 2 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Mucosa, Inflammation, Acute | | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Harderian Gland | + + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Hydronephrosis | | Nephropathy | 2 2 1 2 1 2 2 1 1 2 2 2 2 3 | Renal Tubule, Regeneration | 1 1 2 2 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 43 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 2| 2| 7| 7| 7| 2| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| | | DAY ON TEST | 2| 7| 7| 3| 3| 3| 7| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| | | | 5| 6| 6| 2| 2| 2| 6| 7| 2| 2| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | A | 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + M + + + + | 62 | Artery, Inflammation, Chronic Active | | 1 3.0| __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + | 67 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 70 | Basophilic Focus | | 1 | Clear Cell Focus | X | 2 | Eosinophilic Focus | | 1 | Hypertrophy | | 1 2.0| Infarct | 4 | 3 4.0| Necrosis | | 2 2.0| Vacuolization Cytoplasmic | 1 2 2 2 2 2 2 | 27 2.0| Bile Duct, Hyperplasia | | 1 2.0| Centrilobular, Vacuolization | | | Cytoplasmic | 3 3 3 | 7 3.0| __________________________________________________________________________|____________| Mesentery | | 2 | Fat, Inflammation, Chronic Active | | 2 2.5| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 70 | Acinus, Atrophy | | 2 2.0| Duct, Cyst | | 2 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia, Focal, Squamous | | 2 2.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + | 70 | Spindle Cell, Vacuolization | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 44 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 2| 2| 7| 7| 7| 2| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| | | DAY ON TEST | 2| 7| 7| 3| 3| 3| 7| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| | | | 5| 6| 6| 2| 2| 2| 6| 7| 2| 2| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | A | 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Cytoplasmic | | 1 3.0| __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + | 69 | Accessory Adrenal Cortical Nodule | | 1 | Atrophy | 2 | 1 2.0| Hyperplasia | | 3 2.0| Hypertrophy | 2 2 2 1 2 2 3 2 | 27 2.0| __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia | | 1 2.0| __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 70 | Hyperplasia | | 3 2.0| __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Pituitary Gland | + + + + M I M + + + + + + + + M + + + + | 62 | Pars Distalis, Cyst | | 2 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 70 | Follicle, Cyst | | 1 | Follicle, Dilatation | | 2 1.0| Follicular Cell, Hyperplasia, Focal | 1 | 5 1.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + | 33 | Inflammation, Chronic Active | 4 | 5 3.4| Duct, Dilatation | 4 3 3 3 3 3 3 | 29 3.1| __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + | 70 | Seminiferous Tubule, Atrophy | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 70 | __________________________________________________________________________|____________| Lymph Node | M + + + + + + + + + + + + + + + + + + | 67 | __________________________________________________________________________|____________| Lymph Node, Mandibular | M + + + + + + M + + + + + + + + + + | 65 | Depletion Lymphoid | | 1 2.0| __________________________________________________________________________|____________| Lymph Node, Mesenteric | M + + + + + M + + + + + + + M + M + + | 56 | Hematopoietic Cell Proliferation | 3 | 1 3.0| Infiltration Cellular, Plasma Cell | | 1 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 45 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 2| 2| 7| 7| 7| 2| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| | | DAY ON TEST | 2| 7| 7| 3| 3| 3| 7| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| | | | 5| 6| 6| 2| 2| 2| 6| 7| 2| 2| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | A | 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 69 | Depletion Lymphoid | 2 | 1 2.0| Necrosis | | 1 3.0| __________________________________________________________________________|____________| Thymus | M M + + M + + + + M + + + + M + + + M M | 55 | Depletion Lymphoid | | 4 2.5| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M | | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 70 | Alopecia | | 1 | Ulcer | 3 | 1 3.0| Subcutaneous Tissue, Inflammation, | | | Chronic Active | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + M M + + + M M + + + + + + + + + + + + | 60 | __________________________________________________________________________|____________| Peripheral Nerve | | 1 | Sciatic, Demyelination | | 1 2.0| __________________________________________________________________________|____________| Spinal Cord | | 1 | Demyelination | | 1 3.0| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 70 | Inflammation, Chronic Active | | 1 2.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 70 | Mucosa, Inflammation, Acute | | 1 2.0| __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Harderian Gland | | 6 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 70 | Hydronephrosis | | 1 4.0| Nephropathy | 4 2 3 2 2 4 2 1 1 1 2 3 3 2 2 | 47 2.1| Renal Tubule, Regeneration | 2 | 10 1.5| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 46 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 2| 2| 7| 7| 7| 2| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 7| | | DAY ON TEST | 2| 7| 7| 3| 3| 3| 7| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| | | | 5| 6| 6| 2| 2| 2| 6| 7| 2| 2| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| | A | 0PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + M + + + + + + + + | 68 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 47 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 4| 7| 7| 2| 7| 7| 7| 2| 2| 7| 7| 4| 4| 7| 4| 7| 2| 7| 5| 7| 7| 7| 2| 2| DAY ON TEST | 2| 5| 2| 2| 7| 2| 2| 2| 7| 7| 2| 2| 5| 5| 2| 5| 3| 7| 3| 8| 3| 3| 3| 7| 7| | 9| 2| 9| 9| 6| 9| 9| 9| 7| 7| 9| 9| 3| 3| 9| 3| 0| 7| 0| 0| 0| 0| 0| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + | __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + | Clear Cell Focus | X X | Eosinophilic Focus | | Infarct | 4 | Inflammation, Chronic Active | 2 | Vacuolization Cytoplasmic | 3 3 2 1 3 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + | Acinus, Atrophy | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + | Hyperplasia, Focal, Squamous | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + | Hyperplasia | 3 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + | Hyperplasia | 2 | Hypertrophy | 1 1 1 2 2 1 2 2 2 1 | Vacuolization Cytoplasmic | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + | Hyperplasia | 2 2 | __________________________________________________________________________| Parathyroid Gland | M + + + + + + + + + + + + + | Cyst | | __________________________________________________________________________| Pituitary Gland | + M + M + + + + + + + + + + | Pars Distalis, Cyst | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + | Follicle, Cyst | X | Follicle, Dilatation | | _____________________________________________________________________________________________________________________| Page 48 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 4| 7| 7| 2| 7| 7| 7| 2| 2| 7| 7| 4| 4| 7| 4| 7| 2| 7| 5| 7| 7| 7| 2| 2| DAY ON TEST | 2| 5| 2| 2| 7| 2| 2| 2| 7| 7| 2| 2| 5| 5| 2| 5| 3| 7| 3| 8| 3| 3| 3| 7| 7| | 9| 2| 9| 9| 6| 9| 9| 9| 7| 7| 9| 9| 3| 3| 9| 3| 0| 7| 0| 0| 0| 0| 0| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Follicular Cell, Hyperplasia, Focal | 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + | __________________________________________________________________________| Preputial Gland | + + + + + + + + | Duct, Dilatation | 3 4 3 3 3 4 4 3 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + | Inflammation, Chronic Active | 3 | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + | Seminiferous Tubule, Atrophy | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + M + + + + M + + | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + | Hematopoietic Cell Proliferation | 2 | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + | Hematopoietic Cell Proliferation | | __________________________________________________________________________| Thymus | + + + + + + + + + M + + + + | Depletion Lymphoid | 2 | Infiltration Cellular, | | Polymorphonuclear | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + | Alopecia | X | Ulcer | | Subcutaneous Tissue, Inflammation, | | Chronic Active | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| Page 49 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 4| 7| 7| 2| 7| 7| 7| 2| 2| 7| 7| 4| 4| 7| 4| 7| 2| 7| 5| 7| 7| 7| 2| 2| DAY ON TEST | 2| 5| 2| 2| 7| 2| 2| 2| 7| 7| 2| 2| 5| 5| 2| 5| 3| 7| 3| 8| 3| 3| 3| 7| 7| | 9| 2| 9| 9| 6| 9| 9| 9| 7| 7| 9| 9| 3| 3| 9| 3| 0| 7| 0| 0| 0| 0| 0| 6| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Harderian Gland | | Hyperplasia | | Acinus, Dilatation | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + | Hydronephrosis | | Nephropathy | 3 2 2 2 1 1 2 1 3 2 2 3 4 3 | Cortex, Cyst | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| Page 50 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 4| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 4| 2| 7| 4| 6| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 5| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 3| 5| 7| 3| 5| 7| 3| 3| 3| 3| 3| 0| 3| | 0| 2| 0| 0| 0| 1| 2| 1| 1| 1| 1| 1| 1| 2| 6| 1| 2| 9| 1| 2| 2| 2| 2| 8| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 1500 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + +| Clear Cell Focus | X X X X X | Eosinophilic Focus | X X X X | Infarct | 3 | Inflammation, Chronic Active | | Vacuolization Cytoplasmic | 2 2 3 2 2 3 2 1 2 3 2 2| __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 2 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 | Hypertrophy | 2 2 1 2 1 2 1 2 2 2 2 2 2 2 2 2| Vacuolization Cytoplasmic | | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 3 | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + +| Cyst | X X| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + M| Pars Distalis, Cyst | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + +| Follicle, Cyst | X X X X X| Follicle, Dilatation | | _____________________________________________________________________________________________________________________| Page 51 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 4| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 4| 2| 7| 4| 6| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 5| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 3| 5| 7| 3| 5| 7| 3| 3| 3| 3| 3| 0| 3| | 0| 2| 0| 0| 0| 1| 2| 1| 1| 1| 1| 1| 1| 2| 6| 1| 2| 9| 1| 2| 2| 2| 2| 8| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 1500 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Follicular Cell, Hyperplasia, Focal | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + | Duct, Dilatation | 3 3 3 3 3 3 3 3 3 4 3 3 3 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 4 | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + +| Seminiferous Tubule, Atrophy | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + M + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 2 2 3 | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 4 4 4 | __________________________________________________________________________| Thymus | + M + + + + + + + + + M M + + + + + M +| Depletion Lymphoid | 2 | Infiltration Cellular, | | Polymorphonuclear | 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + +| Alopecia | X | Ulcer | 4 3 2 | Subcutaneous Tissue, Inflammation, | | Chronic Active | 3 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 52 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 4| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| 4| 2| 7| 4| 6| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 5| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 3| 5| 7| 3| 5| 7| 3| 3| 3| 3| 3| 0| 3| | 0| 2| 0| 0| 0| 1| 2| 1| 1| 1| 1| 1| 1| 2| 6| 1| 2| 9| 1| 2| 2| 2| 2| 8| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 1500 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Harderian Gland | + + | Hyperplasia | | Acinus, Dilatation | 3 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + +| Hydronephrosis | | Nephropathy | 2 2 2 2 3 2 3 3 2 1 2 3 1 1 1 2 1 1 2| Cortex, Cyst | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 53 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 2| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 4| 6| 7| 2| 7| 4| 2| | | DAY ON TEST | 7| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 0| 7| 3| 5| 7| | | | 7| 2| 2| 2| 7| 2| 3| 3| 3| 3| 3| 3| 3| 3| 7| 1| 6| 1| 3| 7| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | A | 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Gallbladder | + + + + + A + + + + + + + M + | 47 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + | 59 | Clear Cell Focus | X X X X | 11 | Eosinophilic Focus | X X | 6 | Infarct | | 2 3.5| Inflammation, Chronic Active | | 1 2.0| Vacuolization Cytoplasmic | 2 2 2 2 | 21 2.2| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + | 49 | Acinus, Atrophy | 4 2 | 2 3.0| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 49 | Hyperplasia, Focal, Squamous | | 1 2.0| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 49 | Hyperplasia | | 1 3.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 49 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + | 49 | Hyperplasia | | 3 2.0| Hypertrophy | 2 2 2 2 2 2 1 2 2 2 2 | 37 1.8| Vacuolization Cytoplasmic | 2 | 1 2.0| __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + | 47 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + | 49 | Hyperplasia | 2 | 5 2.4| __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + | 48 | Cyst | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 54 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 2| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 4| 6| 7| 2| 7| 4| 2| | | DAY ON TEST | 7| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 0| 7| 3| 5| 7| | | | 7| 2| 2| 2| 7| 2| 3| 3| 3| 3| 3| 3| 3| 3| 7| 1| 6| 1| 3| 7| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | A | 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + M + + M + + + + + | 44 | Pars Distalis, Cyst | | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 51 | Follicle, Cyst | X | 7 | Follicle, Dilatation | 2 1 | 2 1.5| Follicular Cell, Hyperplasia, Focal | 2 | 2 1.5| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Preputial Gland | + + + + + | 26 | Duct, Dilatation | 3 3 3 3 | 25 3.2| __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + | 49 | Inflammation, Chronic Active | | 2 3.5| __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + | 49 | Seminiferous Tubule, Atrophy | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + | 43 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + M + + + + + + + | 46 | Hematopoietic Cell Proliferation | 2 2 | 7 2.1| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 49 | Hematopoietic Cell Proliferation | 2 | 4 3.5| __________________________________________________________________________|____________| Thymus | + + M + + + + + + + + + + M + | 42 | Depletion Lymphoid | 2 3 3 | 5 2.4| Infiltration Cellular, | | | Polymorphonuclear | | 1 3.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M A M M M M M M M M M M M M | | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 49 | Alopecia | | 2 | Ulcer | | 3 3.0| Subcutaneous Tissue, Inflammation, | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 55 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 2| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 4| 6| 7| 2| 7| 4| 2| | | DAY ON TEST | 7| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 5| 1| 0| 7| 3| 5| 7| | | | 7| 2| 2| 2| 7| 2| 3| 3| 3| 3| 3| 3| 3| 3| 7| 1| 6| 1| 3| 7| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| | A | 1500 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | Chronic Active | | 1 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 49 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 49 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + | 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | 1 | __________________________________________________________________________|____________| Harderian Gland | + + | 4 | Hyperplasia | 3 | 1 3.0| Acinus, Dilatation | | 1 3.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 49 | Hydronephrosis | 2 | 1 2.0| Nephropathy | 2 2 1 2 1 2 3 3 2 2 3 3 1 3 1 | 48 2.0| Cortex, Cyst | X | 3 | __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 56 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 7| 2| 2| 2| 4| 7| 2| 7| 7| 2| 7| 7| 7| 7| 7| 7| 4| 2| 7| 4| DAY ON TEST | 5| 2| 2| 2| 2| 2| 7| 7| 7| 5| 2| 7| 2| 2| 7| 2| 2| 3| 3| 3| 3| 1| 7| 3| 5| | 3| 9| 9| 7| 9| 9| 6| 7| 6| 2| 9| 6| 9| 9| 7| 9| 9| 0| 0| 0| 0| 7| 7| 0| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + A + | __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + A + | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + A + | __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + A + | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + A + | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + A + | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + A + | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Clear Cell Focus | X X X | Eosinophilic Focus | | Hepatodiaphragmatic Nodule | X | Infarct | | Vacuolization Cytoplasmic | 2 2 1 3 3 2 1 2 | __________________________________________________________________________| Mesentery | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + A + | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + | Hyperplasia, Focal, Squamous | 2 1 1 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + | Dysplasia | | __________________________________________________________________________| Tooth | + | Incisor, Abscess | 3 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + | Spindle Cell, Accessory Adrenal | | Cortical Nodule | | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + | Hyperplasia | 1 | Hypertrophy | 1 1 1 2 3 1 1 2 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + A + | Hyperplasia | | __________________________________________________________________________| Parathyroid Gland | + M + + + + + + + + + + + + + + | __________________________________________________________________________| Pituitary Gland | + + + + + + I + + + + + + + M + | Pars Intermedia, Hyperplasia, Focal | | _____________________________________________________________________________________________________________________| Page 57 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 7| 2| 2| 2| 4| 7| 2| 7| 7| 2| 7| 7| 7| 7| 7| 7| 4| 2| 7| 4| DAY ON TEST | 5| 2| 2| 2| 2| 2| 7| 7| 7| 5| 2| 7| 2| 2| 7| 2| 2| 3| 3| 3| 3| 1| 7| 3| 5| | 3| 9| 9| 7| 9| 9| 6| 7| 6| 2| 9| 6| 9| 9| 7| 9| 9| 0| 0| 0| 0| 7| 7| 0| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Pars Intermedia, Hyperplasia | 2 | Rathke's Cleft, Cyst | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + | Inflammation, Chronic Active | | Follicle, Cyst | X | Follicle, Dilatation | 1 | Follicular Cell, Hyperplasia, Focal | 1 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Preputial Gland | + + + + + | Inflammation, Chronic Active | | Duct, Dilatation | 3 3 4 3 3 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + | Granuloma Sperm | | Interstitial Cell, Hyperplasia | | Seminiferous Tubule, Atrophy | 3 | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + M + | Hematopoietic Cell Proliferation | 3 3 | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + A + | Hematopoietic Cell Proliferation | | __________________________________________________________________________| Thymus | + + + + + + I + + + + + + M + + | Depletion Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + | Alopecia | | Ulcer | 2 | Subcutaneous Tissue, Inflammation, | | Chronic Active | 2 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| Page 58 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 7| 2| 2| 2| 4| 7| 2| 7| 7| 2| 7| 7| 7| 7| 7| 7| 4| 2| 7| 4| DAY ON TEST | 5| 2| 2| 2| 2| 2| 7| 7| 7| 5| 2| 7| 2| 2| 7| 2| 2| 3| 3| 3| 3| 1| 7| 3| 5| | 3| 9| 9| 7| 9| 9| 6| 7| 6| 2| 9| 6| 9| 9| 7| 9| 9| 0| 0| 0| 0| 7| 7| 0| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + | Alveolar Epithelium, Hyperplasia | 2 2 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | Cornea, Inflammation, Chronic Active | | __________________________________________________________________________| Harderian Gland | + | Hyperplasia | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + | Nephropathy | 2 2 2 3 3 2 2 2 2 2 2 2 2 3 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + | _____________________________________________________________________________________________________________________| Page 59 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 2| 4| 7| 7| 7| 2| 7| 2| 7| 7| 4| 7| 7| 7| 7| 7| 4| 7| 7| DAY ON TEST | 0| 3| 3| 3| 3| 3| 5| 5| 3| 3| 3| 7| 3| 7| 3| 3| 5| 3| 3| 3| 3| 3| 5| 3| 3| | 2| 0| 0| 0| 0| 0| 1| 3| 1| 1| 1| 6| 1| 7| 1| 1| 3| 1| 1| 1| 2| 2| 3| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 5000 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Clear Cell Focus | X X | Eosinophilic Focus | X | Hepatodiaphragmatic Nodule | | Infarct | 2 | Vacuolization Cytoplasmic | 2 2 2 2 2 1 2 1 3 | __________________________________________________________________________| Mesentery | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal, Squamous | 1 1 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + +| Dysplasia | | __________________________________________________________________________| Tooth | | Incisor, Abscess | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + +| Spindle Cell, Accessory Adrenal | | Cortical Nodule | | __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | Hypertrophy | 2 2 1 1 1 1 2 2 1 2 1 2 2 2 2 2 2 1| __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 | __________________________________________________________________________| Parathyroid Gland | + + M M + + M + + + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + +| Pars Intermedia, Hyperplasia, Focal | 3 | _____________________________________________________________________________________________________________________| Page 60 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 2| 4| 7| 7| 7| 2| 7| 2| 7| 7| 4| 7| 7| 7| 7| 7| 4| 7| 7| DAY ON TEST | 0| 3| 3| 3| 3| 3| 5| 5| 3| 3| 3| 7| 3| 7| 3| 3| 5| 3| 3| 3| 3| 3| 5| 3| 3| | 2| 0| 0| 0| 0| 0| 1| 3| 1| 1| 1| 6| 1| 7| 1| 1| 3| 1| 1| 1| 2| 2| 3| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 5000 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Pars Intermedia, Hyperplasia | | Rathke's Cleft, Cyst | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 2 | Follicle, Cyst | X X X X 1 | Follicle, Dilatation | 1 1 1| Follicular Cell, Hyperplasia, Focal | 1 1 4 1| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Preputial Gland | + + + + + +| Inflammation, Chronic Active | 4 | Duct, Dilatation | 3 3 3 3 2| __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + +| Granuloma Sperm | | Interstitial Cell, Hyperplasia | | Seminiferous Tubule, Atrophy | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | M + M + M + + + + M + + + + + + + + + +| Hematopoietic Cell Proliferation | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + +| Depletion Lymphoid | 3 2 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + +| Alopecia | | Ulcer | | Subcutaneous Tissue, Inflammation, | | Chronic Active | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 61 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 2| 4| 7| 7| 7| 2| 7| 2| 7| 7| 4| 7| 7| 7| 7| 7| 4| 7| 7| DAY ON TEST | 0| 3| 3| 3| 3| 3| 5| 5| 3| 3| 3| 7| 3| 7| 3| 3| 5| 3| 3| 3| 3| 3| 5| 3| 3| | 2| 0| 0| 0| 0| 0| 1| 3| 1| 1| 1| 6| 1| 7| 1| 1| 3| 1| 1| 1| 2| 2| 3| 2| 2| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| ANIMAL ID | 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 5000 PPM | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | 1 | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | Cornea, Inflammation, Chronic Active | | __________________________________________________________________________| Harderian Gland | + | Hyperplasia | 2 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + +| Nephropathy | 1 2 2 2 3 3 2 2 2 2 2 2 2 2 3 2 1 1 1| __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 62 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 2| 7| 2| 7| 4| 7| 7| 7| 7| 7| 4| 7| 5| 7| 7| | | DAY ON TEST | 5| 3| 3| 3| 3| 7| 3| 7| 3| 5| 3| 3| 3| 3| 3| 5| 3| 9| 3| 3| | | | 3| 2| 2| 2| 2| 6| 2| 7| 3| 2| 3| 3| 3| 3| 3| 2| 3| 2| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| | A | 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + | 49 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + | 60 | Basophilic Focus | X X | 4 | Clear Cell Focus | X | 6 | Eosinophilic Focus | X X | 3 | Hepatodiaphragmatic Nodule | | 1 | Infarct | 2 | 2 2.0| Vacuolization Cytoplasmic | 2 1 1 2 2 2 | 23 1.9| __________________________________________________________________________|____________| Mesentery | + | 1 | __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + | 51 | Hyperplasia, Focal, Squamous | | 5 1.2| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + | 51 | Dysplasia | 2 | 1 2.0| __________________________________________________________________________|____________| Tooth | | 1 | Incisor, Abscess | | 1 3.0| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + | 51 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + | 51 | Spindle Cell, Accessory Adrenal | | | Cortical Nodule | X | 1 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + | 51 | Hyperplasia | 2 2 1 | 5 1.6| Hypertrophy | 2 3 2 2 1 1 2 2 1 | 35 1.6| __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + | 48 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 63 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 2| 7| 2| 7| 4| 7| 7| 7| 7| 7| 4| 7| 5| 7| 7| | | DAY ON TEST | 5| 3| 3| 3| 3| 7| 3| 7| 3| 5| 3| 3| 3| 3| 3| 5| 3| 9| 3| 3| | | | 3| 2| 2| 2| 2| 6| 2| 7| 3| 2| 3| 3| 3| 3| 3| 2| 3| 2| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| | A | 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + | 50 | Hyperplasia | 2 | 2 2.5| __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + M + + + + | 46 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + | 49 | Pars Intermedia, Hyperplasia, Focal | | 1 3.0| Pars Intermedia, Hyperplasia | | 1 2.0| Rathke's Cleft, Cyst | | 1 | __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + | 52 | Inflammation, Chronic Active | 1 | 2 1.5| Follicle, Cyst | X | 7 1.0| Follicle, Dilatation | 1 | 5 1.0| Follicular Cell, Hyperplasia, Focal | 2 1 | 8 1.5| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + | 20 | Inflammation, Chronic Active | 4 | 2 4.0| Duct, Dilatation | 3 4 4 4 3 3 3 3 | 18 3.2| __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + | 52 | Granuloma Sperm | 2 | 1 2.0| Interstitial Cell, Hyperplasia | 3 | 1 3.0| Seminiferous Tubule, Atrophy | 4 | 2 3.5| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + | 50 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + M + + + + + + + + + + + + + | 45 | Hematopoietic Cell Proliferation | | 2 3.0| __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + | 50 | Hematopoietic Cell Proliferation | | 1 2.0| __________________________________________________________________________|____________| Thymus | + + + M + + + + + + + + + M + | 47 | Depletion Lymphoid | 2 | 4 2.3| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 64 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 2| 7| 2| 7| 4| 7| 7| 7| 7| 7| 4| 7| 5| 7| 7| | | DAY ON TEST | 5| 3| 3| 3| 3| 7| 3| 7| 3| 5| 3| 3| 3| 3| 3| 5| 3| 9| 3| 3| | | | 3| 2| 2| 2| 2| 6| 2| 7| 3| 2| 3| 3| 3| 3| 3| 2| 3| 2| 3| 3| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| | A | 5000 PPM | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M | | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + | 51 | Alopecia | X | 1 | Ulcer | 4 3 | 3 3.0| Subcutaneous Tissue, Inflammation, | | | Chronic Active | 3 2 | 3 2.3| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + | 51 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + | 51 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + | 51 | Alveolar Epithelium, Hyperplasia | 2 | 4 1.8| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + | 51 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | + | 1 | Cornea, Inflammation, Chronic Active | 3 | 1 3.0| __________________________________________________________________________|____________| Harderian Gland | + | 3 | Hyperplasia | | 1 2.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + | 51 | Nephropathy | 2 1 1 2 2 2 1 2 2 2 2 3 2 | 46 2.0| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + | 51 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 65 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 4| 7| 7| 7| 7| 7| 4| 4| 2| 4| 7| 7| 4| 7| 7| 7| 4| 4| 7| 7| 7| DAY ON TEST | 7| 2| 2| 2| 5| 2| 2| 2| 2| 2| 5| 5| 7| 5| 2| 3| 5| 3| 3| 3| 5| 5| 3| 3| 3| | 6| 9| 9| 9| 3| 9| 9| 9| 9| 9| 2| 3| 6| 2| 9| 0| 3| 0| 0| 0| 2| 2| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Diverticulum | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Clear Cell Focus | X | Eosinophilic Focus | | Infarct | | Inflammation, Chronic Active | | Mineralization, Focal | | Mixed Cell Focus | | Vacuolization Cytoplasmic | 2 1 2 2 1 2 1 1 2 1 1| Centrilobular, Vacuolization | | Cytoplasmic | 2 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy | 2 2 | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Erosion, Focal | 4 | Hyperplasia, Focal, Squamous | 4 2 2 2 3 | Inflammation, Chronic Active | 1 3 2 | Ulcer | 3 3 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + M + + + + +| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | Hypertrophy | 2 2 2 1 1 2 2 2| __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 | _____________________________________________________________________________________________________________________| Page 66 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 4| 7| 7| 7| 7| 7| 4| 4| 2| 4| 7| 7| 4| 7| 7| 7| 4| 4| 7| 7| 7| DAY ON TEST | 7| 2| 2| 2| 5| 2| 2| 2| 2| 2| 5| 5| 7| 5| 2| 3| 5| 3| 3| 3| 5| 5| 3| 3| 3| | 6| 9| 9| 9| 3| 9| 9| 9| 9| 9| 2| 3| 6| 2| 9| 0| 3| 0| 0| 0| 2| 2| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | M + + + + + + + + + + + + + I + + + + + + + + + +| Pars Distalis, Cyst | X| Pars Distalis, Hyperplasia | | Pars Distalis, Karyomegaly | 2 | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | 1 | Follicle, Cyst | X X | Follicle, Dilatation | 1 1 1 2 2 1 1 1 1 1 1| Follicular Cell, Hyperplasia, Focal | 1 3 4 1 2 4 1 1 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | + | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + +| Inflammation, Chronic Active | | Duct, Dilatation | 3 3 3 3 3 4 3 4 3 3 3| __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | + | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + M + + + + + + + + + + + + +| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + M + + + + + M + + + + + +| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 3 | __________________________________________________________________________| Thymus | + + + + + + M + + + + + + M M M I + + + + + + + M| Depletion Lymphoid | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Alopecia | | Ulcer | | Subcutaneous Tissue, Inflammation, | | Chronic Active | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 67 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 4| 7| 7| 7| 7| 7| 4| 4| 2| 4| 7| 7| 4| 7| 7| 7| 4| 4| 7| 7| 7| DAY ON TEST | 7| 2| 2| 2| 5| 2| 2| 2| 2| 2| 5| 5| 7| 5| 2| 3| 5| 3| 3| 3| 5| 5| 3| 3| 3| | 6| 9| 9| 9| 3| 9| 9| 9| 9| 9| 2| 3| 6| 2| 9| 0| 3| 0| 0| 0| 2| 2| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | M + + + + + + + + + M + M M + + + + + + + + + + +| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | __________________________________________________________________________| Harderian Gland | + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic Active | | Nephropathy | 2 2 2 2 2 1 2 2 2 3 2 2 4 3 3 1 1| Renal Tubule, Regeneration | 1 1 1 1 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + M + + + + + + + + + +| Lumen, Hemorrhage | 3 | _____________________________________________________________________________________________________________________| Page 68 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 7| 4| 6| 7| 2| 2| 6| 7| 7| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 1| DAY ON TEST | 3| 3| 3| 5| 3| 3| 5| 3| 3| 7| 7| 4| 3| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 3| 4| | 0| 0| 1| 3| 1| 1| 3| 4| 1| 7| 7| 5| 1| 1| 1| 1| 7| 1| 1| 2| 2| 2| 2| 2| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 15000 | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Large | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Small | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + A| Diverticulum | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + A| Clear Cell Focus | | Eosinophilic Focus | X | Infarct | 4 | Inflammation, Chronic Active | | Mineralization, Focal | 2 | Mixed Cell Focus | | Vacuolization Cytoplasmic | 1 3 1 1 1 1 1 1 | Centrilobular, Vacuolization | | Cytoplasmic | 3 1 | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + M| Acinus, Atrophy | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Stomach | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + A| Erosion, Focal | 2 | Hyperplasia, Focal, Squamous | 3 2 2 2 2 | Inflammation, Chronic Active | 3 1 | Ulcer | 3 2 1 | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + A| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + + + + + A| Hyperplasia | | Hypertrophy | 2 2 2 1 1 1 2 | __________________________________________________________________________| Adrenal Gland, Medulla | + + + + + + + + + + + + + + + + + + + + + + + + A| Cyst | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + M| Hyperplasia | 2 | _____________________________________________________________________________________________________________________| Page 69 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 7| 4| 6| 7| 2| 2| 6| 7| 7| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 1| DAY ON TEST | 3| 3| 3| 5| 3| 3| 5| 3| 3| 7| 7| 4| 3| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 3| 4| | 0| 0| 1| 3| 1| 1| 3| 4| 1| 7| 7| 5| 1| 1| 1| 1| 7| 1| 1| 2| 2| 2| 2| 2| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 15000 | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + M + + M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + M + + + + + + + + M + + + A| Pars Distalis, Cyst | X | Pars Distalis, Hyperplasia | | Pars Distalis, Karyomegaly | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + A| Inflammation, Chronic Active | | Follicle, Cyst | X | Follicle, Dilatation | 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 | Follicular Cell, Hyperplasia, Focal | 1 1 2 1 3 1 1 3 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Preputial Gland | + + + + + + + + + + | Inflammation, Chronic Active | | Duct, Dilatation | 3 3 3 3 3 3 3 3 3 3 | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + A| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Lymph Node, Mesenteric | + + + M + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + M| Hematopoietic Cell Proliferation | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + M + + + + + + M + + M| Depletion Lymphoid | 2 2 2 2 1 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M| __________________________________________________________________________| Skin | + + + + + + + + + M + + + + + + + + + + + + + + A| Alopecia | X | Ulcer | 4 | Subcutaneous Tissue, Inflammation, | | Chronic Active | 2 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 70 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 _____________________________________________________________________________________________________________________ | 7| 7| 7| 4| 7| 7| 4| 6| 7| 2| 2| 6| 7| 7| 7| 7| 2| 7| 7| 7| 7| 7| 7| 7| 1| DAY ON TEST | 3| 3| 3| 5| 3| 3| 5| 3| 3| 7| 7| 4| 3| 3| 3| 3| 7| 3| 3| 3| 3| 3| 3| 3| 4| | 0| 0| 1| 3| 1| 1| 3| 4| 1| 7| 7| 5| 1| 1| 1| 1| 7| 1| 1| 2| 2| 2| 2| 2| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| ANIMAL ID | 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 15000 | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + M + + M M + + + + + + + + + + + + + A| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar Epithelium, Hyperplasia | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + A| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + A| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | __________________________________________________________________________| Harderian Gland | + + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + M| Inflammation, Chronic Active | | Nephropathy | 1 2 1 1 1 2 4 2 1 2 2 2 1 1 1 1 1 | Renal Tubule, Regeneration | 1 | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + A| Lumen, Hemorrhage | | _____________________________________________________________________________________________________________________| Page 71 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 4| 2| 7| 7| 2| 2| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 6| 2| | | DAY ON TEST | 3| 3| 5| 7| 3| 3| 7| 7| 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 4| 7| | | | 2| 2| 2| 7| 2| 2| 7| 6| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 5| 6| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| | A | 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Gallbladder | + + M + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Intestine Large | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + | 69 | Diverticulum | 2 | 1 2.0| __________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + | 69 | Clear Cell Focus | | 1 | Eosinophilic Focus | | 1 | Infarct | 4 | 2 4.0| Inflammation, Chronic Active | 3 | 1 3.0| Mineralization, Focal | | 1 2.0| Mixed Cell Focus | X | 1 | Vacuolization Cytoplasmic | 1 1 2 3 2 | 24 1.5| Centrilobular, Vacuolization | | | Cytoplasmic | | 3 2.0| __________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + | 69 | Acinus, Atrophy | 2 | 3 2.0| __________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Stomach | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + | 69 | Erosion, Focal | | 2 3.0| Hyperplasia, Focal, Squamous | 2 2 2 2 | 14 2.3| Inflammation, Chronic Active | | 5 2.0| Ulcer | 3 | 6 2.5| __________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + | 68 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Gland | + + + + + + + + + + + + + + + + + + + + | 68 | __________________________________________________________________________|____________| Adrenal Gland, Cortex | + + + + + + + + + + + + + + + + + + + + | 68 | Hyperplasia | | 1 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 72 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 4| 2| 7| 7| 2| 2| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 6| 2| | | DAY ON TEST | 3| 3| 5| 7| 3| 3| 7| 7| 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 4| 7| | | | 2| 2| 2| 7| 2| 2| 7| 6| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 5| 6| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| | A | 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Hypertrophy | 2 2 1 2 1 1 2 1 1 2 2 | 26 1.6| __________________________________________________________________________|____________| Adrenal Gland, Medulla | + + + + + + + + + + I + + + + + + + + + | 67 | Cyst | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + | 69 | Hyperplasia | 2 | 3 2.3| __________________________________________________________________________|____________| Parathyroid Gland | + + + + + + M + + + M + + + + + M + + + | 65 | __________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + M + + + + + | 64 | Pars Distalis, Cyst | | 2 | Pars Distalis, Hyperplasia | 1 | 1 1.0| Pars Distalis, Karyomegaly | | 1 2.0| __________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Chronic Active | | 1 1.0| Follicle, Cyst | X X | 5 | Follicle, Dilatation | 1 1 1 1 1 1 1 2 1 | 38 1.1| Follicular Cell, Hyperplasia, Focal | 2 1 1 3 3 2 2 2 2 | 27 1.9| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | __________________________________________________________________________|____________| Tissue NOS | | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + | 31 | Inflammation, Chronic Active | 4 | 1 4.0| Duct, Dilatation | 3 3 3 3 3 3 3 3 3 | 30 3.1| __________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + | 61 | __________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Blood | | 1 | __________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Lymph Node | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + M + + + + + + + + + + + + | 65 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + M + + + + + + | 63 | __________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + | 69 | Hematopoietic Cell Proliferation | 3 | 2 3.0| __________________________________________________________________________|____________| Thymus | + + + + + + + + + + + M + + + + + M + + | 59 | Depletion Lymphoid | | 5 1.8| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 73 NTP Experiment-Test: 05085-02 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC MANGANESE SULFATE Date: 04/24/97 Route: DOSED FEED Time: 17:42:42 __________________________________________________________________________________________________________________________________ | 7| 7| 4| 2| 7| 7| 2| 2| 7| 7| 4| 7| 7| 7| 7| 7| 7| 7| 6| 2| | | DAY ON TEST | 3| 3| 5| 7| 3| 3| 7| 7| 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 4| 7| | | | 2| 2| 2| 7| 2| 2| 7| 6| 3| 3| 2| 3| 3| 3| 3| 3| 3| 3| 5| 6| | T (*) | _____________________________________________________________________________________________________________________| | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | B6C3F1 MICE MALE | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | T | ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| | A | 15000 | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | L | PPM | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | | | | __________________________________________________________________________|____________| Mammary Gland | M M + M M M M M M M M M M M M M M M | 1 | __________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + | 68 | Alopecia | X X | 3 | Ulcer | | 1 4.0| Subcutaneous Tissue, Inflammation, | | | Chronic Active | | 1 2.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + | 70 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | + + M + + + M + + + M + + + + + + + + + | 59 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + | 69 | Alveolar Epithelium, Hyperplasia | 2 | 1 2.0| __________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + | 69 | __________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + | 69 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | + | 2 | __________________________________________________________________________|____________| Harderian Gland | + + | 5 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + | 69 | Inflammation, Chronic Active | 4 | 1 4.0| Nephropathy | 2 2 2 2 2 1 1 1 2 2 1 2 2 | 47 1.8| Renal Tubule, Regeneration | 1 | 6 1.0| __________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + | 68 | Lumen, Hemorrhage | | 1 3.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 74 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------