TDMS Study 05096-01 Pathology Tables
INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
0-CHLOROBENZALMALONONITRILE (CS-2)
NTP Experiment-Test: 05096-01 Report: PEIRPT03
Study Type: CHRONIC Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
Facility: Battelle Northwest
Chemical CAS #: 2698-41-1
Lock Date: None
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Dead 7 5 9 4
Moribund 23 21 12 19
Survivors
Terminal Sacrifice 20 24 29 27
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Intestine Large, Cecum (40) (20) (10) (46)
Inflammation, Suppurative 1 (5%)
Parasite Metazoan 4 (10%) 1 (5%) 1 (10%) 6 (13%)
Intestine Large, Colon (45) (20) (15) (47)
Parasite Metazoan 2 (4%) 1 (5%) 2 (13%) 5 (11%)
Intestine Large, Rectum (47) (23) (14) (43)
Parasite Metazoan 1 (2%) 1 (4%) 3 (7%)
Intestine Small, Duodenum (43) (24) (15) (46)
Ulcer 1 (7%) 1 (2%)
Intestine Small, Ileum (31) (18) (4) (40)
Hyperplasia, Lymphoid 1 (3%) 3 (8%)
Parasite Metazoan 1 (3%)
Intestine Small, Jejunum (37) (13) (7) (38)
Hyperplasia, Lymphoid 1 (3%)
Liver (49) (50) (50) (50)
Angiectasis 5 (10%) 6 (12%) 6 (12%) 7 (14%)
Basophilic Focus 25 (51%) 18 (36%) 24 (48%) 22 (44%)
Clear Cell Focus 2 (4%) 2 (4%) 1 (2%)
Congestion 1 (2%) 1 (2%) 1 (2%)
Degeneration, Fatty 18 (37%) 19 (38%) 8 (16%) 11 (22%)
Eosinophilic Focus 2 (4%)
Hematopoietic Cell Proliferation 7 (14%) 5 (10%) 9 (18%) 1 (2%)
Hemorrhage 1 (2%)
Hepatodiaphragmatic Nodule 6 (12%) 2 (4%) 8 (16%) 11 (22%)
Hyperplasia 3 (6%)
Inflammation, Focal, Granulomatous 23 (47%) 23 (46%) 26 (52%) 28 (56%)
Leukocytosis 2 (4%) 1 (2%) 1 (2%)
Necrosis 10 (20%) 13 (26%) 9 (18%) 9 (18%)
Pigmentation, Bile 1 (2%)
Thrombosis 1 (2%) 1 (2%)
Bile Duct, Hyperplasia 8 (16%) 10 (20%) 6 (12%) 8 (16%)
Mesentery (4) (4) (2)
Fat, Hemorrhage 1 (25%)
Fat, Inflammation, Chronic 3 (75%) 3 (75%) 2 (100%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Fat, Necrosis 3 (75%) 3 (75%) 2 (100%)
Pancreas (49) (25) (19) (50)
Acinus, Atrophy 11 (22%) 3 (12%) 5 (26%) 10 (20%)
Acinus, Fibrosis 1 (4%)
Acinus, Focal Cellular Change 1 (4%)
Acinus, Inflammation 1 (2%)
Pharynx (1) (1)
Palate, Developmental Malformation 1 (100%)
Palate, Inflammation 1 (100%)
Salivary Glands (49) (25) (19) (50)
Inflammation, Chronic 2 (4%)
Inflammation, Suppurative 16 (33%) 1 (4%) 3 (16%) 13 (26%)
Duct, Hyperplasia 22 (45%) 8 (32%) 7 (37%) 25 (50%)
Stomach, Forestomach (48) (33) (21) (50)
Inflammation, Chronic 3 (6%) 9 (27%) 1 (5%) 4 (8%)
Inflammation, Suppurative 4 (8%) 2 (6%) 1 (5%) 1 (2%)
Ulcer 5 (10%) 10 (30%) 2 (10%) 5 (10%)
Epithelium, Hyperplasia 10 (21%) 10 (30%) 2 (10%) 7 (14%)
Stomach, Glandular (49) (31) (20) (50)
Hemorrhage 2 (4%) 1 (5%) 1 (2%)
Inflammation, Chronic 5 (10%) 4 (13%) 1 (2%)
Inflammation, Suppurative 2 (4%) 1 (3%) 1 (5%) 1 (2%)
Pigmentation, Hemosiderin 1 (2%) 1 (3%) 1 (5%) 1 (2%)
Ulcer 9 (18%) 4 (13%) 3 (15%) 3 (6%)
Epithelium, Hyperplasia 1 (2%) 1 (2%)
Tongue (1) (1)
Epithelium, Hyperplasia 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (49) (28) (21) (50)
Cardiomyopathy 42 (86%) 21 (75%) 16 (76%) 49 (98%)
Inflammation, Suppurative 1 (4%)
Mineralization 1 (4%)
Necrosis 1 (4%)
Atrium, Congestion 2 (7%)
Atrium, Inflammation 1 (2%)
Atrium, Thrombosis 3 (6%) 1 (4%) 1 (5%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland, Cortex (49) (25) (23) (48)
Degeneration, Fatty 26 (53%) 12 (48%) 7 (30%) 29 (60%)
Ectopic Tissue 1 (2%)
Focal Cellular Change 6 (12%) 2 (8%) 8 (17%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Hematopoietic Cell Proliferation 10 (20%) 4 (16%) 3 (13%) 10 (21%)
Hyperplasia 7 (14%) 3 (13%) 4 (8%)
Necrosis 3 (6%) 1 (4%) 1 (4%)
Thrombosis 1 (4%)
Adrenal Gland, Medulla (37) (21) (18) (44)
Hyperplasia 10 (27%) 2 (10%) 7 (39%) 8 (18%)
Thrombosis 1 (6%)
Islets, Pancreatic (48) (24) (20) (49)
Hyperplasia 1 (2%)
Parathyroid Gland (40) (22) (18) (43)
Hyperplasia 3 (8%) 2 (9%) 1 (2%)
Pituitary Gland (48) (44) (42) (49)
Degeneration, Cystic 3 (7%) 1 (2%)
Pars Distalis, Angiectasis 3 (6%) 2 (5%) 1 (2%)
Pars Distalis, Cyst 3 (6%) 1 (2%)
Pars Distalis, Hemorrhage 2 (5%)
Pars Distalis, Hyperplasia 10 (21%) 6 (14%) 6 (14%) 7 (14%)
Thyroid Gland (48) (26) (18) (50)
C-Cell, Hyperplasia 10 (21%) 4 (15%) 4 (8%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (48) (25) (21) (48)
Cyst 2 (4%)
Hyperplasia 1 (2%) 1 (5%) 2 (4%)
Inflammation, Suppurative 11 (23%) 1 (4%) 3 (14%) 7 (15%)
Duct, Hyperplasia 1 (2%)
Ovary (49) (49) (50) (50)
Atrophy 1 (2%) 2 (4%) 1 (2%) 4 (8%)
Cyst 2 (4%) 3 (6%) 1 (2%) 6 (12%)
Hyperplasia 1 (2%)
Proliferation 1 (2%)
Uterus (49) (31) (23) (50)
Dilatation 2 (4%) 2 (4%)
Hemorrhage 1 (4%) 2 (4%)
Hyperplasia, Cystic 1 (2%) 1 (2%)
Submucosa, Hyperplasia 1 (3%)
Vagina (1) (1)
Inflammation, Suppurative 1 (100%) 1 (100%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (49) (26) (19) (49)
Depletion Cellular 1 (2%)
Hyperplasia, Neutrophil 2 (4%)
Inflammation, Focal, Granulomatous 1 (2%)
Myelofibrosis 3 (6%) 1 (4%) 1 (5%) 2 (4%)
Lymph Node (49) (48) (49) (50)
Mediastinal, Inflammation, Focal,
Granulomatous 1 (2%)
Mesenteric, Inflammation, Focal,
Granulomatous 1 (2%)
Lymph Node, Bronchial (47) (47) (47) (46)
Congestion 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hyperplasia 4 (9%) 3 (6%) 10 (22%)
Inflammation, Focal, Granulomatous 1 (2%) 1 (2%)
Lymph Node, Mandibular (45) (25) (19) (47)
Congestion 1 (2%)
Hyperplasia 15 (33%) 3 (12%) 2 (11%) 19 (40%)
Inflammation, Focal, Granulomatous 1 (2%)
Necrosis 1 (5%)
Spleen (49) (50) (49) (50)
Developmental Malformation 1 (2%)
Fibrosis 5 (10%) 2 (4%) 3 (6%) 5 (10%)
Hematopoietic Cell Proliferation 6 (12%) 4 (8%) 7 (14%) 2 (4%)
Hemorrhage 3 (6%) 1 (2%)
Inflammation, Granulomatous 1 (2%) 1 (2%) 2 (4%)
Necrosis 2 (4%) 1 (2%)
Pigmentation, Hemosiderin 1 (2%) 3 (6%)
Thrombosis 3 (6%)
Capsule, Inflammation, Suppurative 1 (2%)
Capsule, Thrombosis 1 (2%)
Thymus (44) (23) (20) (47)
Degeneration, Cystic 2 (4%)
Inflammation, Chronic 1 (5%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (48) (50) (49) (49)
Galactocele 2 (4%) 3 (6%) 1 (2%)
Hyperplasia 6 (13%) 9 (18%) 10 (20%) 11 (22%)
Skin (50) (33) (30) (50)
Acanthosis 1 (3%)
Cyst Epithelial Inclusion 1 (3%)
Inflammation, Suppurative 1 (3%)
Necrosis 1 (3%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM - CONT
Ulcer 2 (6%)
Epidermis, Hyperplasia 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic 2 (6%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (29) (21) (50)
Fibrous Osteodystrophy 3 (6%) 1 (3%)
Osteopetrosis 4 (8%) 2 (7%) 1 (5%) 6 (12%)
Periosteum, Proliferation 18 (36%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (49) (28) (21) (50)
Hemorrhage 6 (12%) 4 (14%) 3 (14%) 3 (6%)
Hydrocephalus 3 (6%) 1 (4%) 1 (2%)
Necrosis 1 (2%)
Meninges, Inflammation, Suppurative 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (49) (24) (18) (50)
Inflammation 2 (11%)
Inflammation, Suppurative 22 (45%) 10 (42%) 6 (33%) 22 (44%)
Metaplasia, Squamous 1 (2%) 1 (4%) 2 (4%)
Epithelium, Hyperplasia 1 (2%)
Lung (49) (50) (50) (50)
Congestion 2 (4%) 4 (8%) 5 (10%) 3 (6%)
Edema 1 (2%)
Hemorrhage 3 (6%) 1 (2%) 3 (6%) 8 (16%)
Infiltration Cellular, Mixed Cell 3 (6%) 1 (2%) 2 (4%)
Inflammation, Chronic, Focal 16 (33%) 7 (14%) 24 (48%) 32 (64%)
Inflammation, Focal, Granulomatous 2 (4%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Alveolar Epithelium, Hyperplasia 4 (8%) 4 (8%) 1 (2%) 5 (10%)
Alveolus, Infiltration Cellular, Histiocyte 6 (12%) 4 (8%) 5 (10%) 20 (40%)
Mediastinum, Inflammation, Chronic 1 (2%)
Perivascular, Infiltration Cellular,
Mononuclear Cell 18 (37%) 10 (20%) 21 (42%) 23 (46%)
Nose (49) (49) (49) (50)
Inflammation 37 (76%) 21 (43%) 34 (69%) 48 (96%)
Inflammation, Suppurative 46 (94%) 30 (61%) 43 (88%) 47 (94%)
Thrombosis 8 (16%) 5 (10%) 3 (6%)
Nares, Inflammation, Chronic 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Nasolacrimal Duct, Inflammation, Suppurative 14 (29%) 13 (27%) 11 (22%) 7 (14%)
Olfactory Epithelium, Degeneration 1 (2%) 23 (46%)
Olfactory Epithelium, Metaplasia 3 (6%) 1 (2%) 1 (2%) 15 (30%)
Olfactory Epithelium, Metaplasia, Squamous 3 (6%)
Respiratory Epithelium, Hyperplasia 3 (6%) 3 (6%) 6 (12%) 46 (92%)
Respiratory Epithelium, Metaplasia, Squamous 2 (4%) 5 (10%) 49 (98%)
Submucosa, Hyperplasia 5 (10%)
Vomeronasal Organ, Inflammation, Suppurative 12 (24%) 4 (8%) 6 (12%) 12 (24%)
Trachea (48) (25) (18) (48)
Inflammation, Suppurative 3 (6%) 2 (8%) 2 (11%) 3 (6%)
Epithelium, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Ear (1)
Inflammation, Suppurative 1 (100%)
Eye (48) (5) (3) (49)
Synechia 2 (40%) 3 (6%)
Anterior Chamber, Inflammation, Suppurative 1 (2%)
Cornea, Hyperplasia 1 (2%)
Cornea, Inflammation, Suppurative 1 (33%) 3 (6%)
Lens, Degeneration 5 (10%) 3 (60%) 1 (33%) 5 (10%)
Lens, Mineralization 2 (4%)
Retina, Degeneration 4 (8%) 3 (60%) 1 (33%) 6 (12%)
Harderian Gland (4) (1) (1)
Inflammation, Suppurative 4 (100%)
Metaplasia, Squamous 1 (100%) 1 (100%)
Lacrimal Gland (3) (3) (7) (7)
Inflammation, Suppurative 1 (33%) 1 (33%)
Acinus, Atrophy 3 (100%) 3 (100%) 7 (100%) 7 (100%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (49) (37) (30) (50)
Cyst 1 (3%)
Hematopoietic Cell Proliferation 1 (2%) 1 (3%)
Inflammation, Suppurative 1 (3%)
Mineralization 1 (2%) 1 (3%)
Nephropathy 45 (92%) 34 (92%) 29 (97%) 48 (96%)
Artery, Hyperplasia 1 (3%)
Capsule, Inflammation 1 (3%)
Renal Tubule, Hyperplasia 3 (6%) 2 (5%) 1 (3%) 1 (2%)
Urinary Bladder (47) (24) (20) (48)
Hemorrhage 2 (4%)
Inflammation, Suppurative 1 (5%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS FEMALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Transitional Epithelium, Hyperplasia 1 (2%)
Vein, Ectasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 50 50 50 50
Early Deaths
Moribund 19 27 21 20
Dead 5 6 8 4
Survivors
Terminal Sacrifice 26 17 21 26
Animals Examined Microscopically 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (49) (33) (28) (48)
Inflammation, Suppurative 1 (4%)
Intestine Large, Cecum (44) (26) (21) (44)
Hemorrhage 1 (2%)
Inflammation, Suppurative 2 (5%)
Parasite Metazoan 4 (9%) 1 (5%) 6 (14%)
Intestine Large, Colon (48) (32) (24) (46)
Parasite Metazoan 6 (13%) 7 (22%) 3 (13%) 8 (17%)
Intestine Large, Rectum (45) (30) (20) (45)
Inflammation, Suppurative 1 (2%)
Parasite Metazoan 3 (7%) 2 (7%) 4 (9%)
Ulcer 1 (2%)
Intestine Small, Ileum (42) (22) (18) (43)
Hyperplasia, Lymphoid 9 (21%) 4 (9%)
Parasite Metazoan 1 (2%)
Intestine Small, Jejunum (41) (18) (12) (39)
Parasite Metazoan 1 (3%)
Liver (50) (50) (49) (50)
Angiectasis 3 (6%) 5 (10%) 8 (16%) 3 (6%)
Basophilic Focus 17 (34%) 7 (14%) 10 (20%) 23 (46%)
Clear Cell Focus 9 (18%) 2 (4%) 3 (6%) 4 (8%)
Congestion 1 (2%)
Degeneration 3 (6%) 2 (4%)
Degeneration, Cystic 1 (2%) 7 (14%) 7 (14%) 3 (6%)
Degeneration, Fatty 16 (32%) 24 (48%) 11 (22%) 10 (20%)
Eosinophilic Focus 1 (2%)
Hematopoietic Cell Proliferation 1 (2%) 1 (2%) 5 (10%) 5 (10%)
Hepatodiaphragmatic Nodule 7 (14%) 5 (10%) 7 (14%) 9 (18%)
Hyperplasia 1 (2%) 2 (4%)
Inflammation, Focal, Granulomatous 14 (28%) 13 (26%) 8 (16%) 8 (16%)
Leukocytosis 5 (10%) 1 (2%) 1 (2%) 1 (2%)
Necrosis 8 (16%) 10 (20%) 11 (22%) 7 (14%)
Thrombosis 1 (2%)
Bile Duct, Hyperplasia 40 (80%) 32 (64%) 31 (63%) 31 (62%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Mesentery (5) (3) (2) (4)
Hemorrhage 1 (50%)
Fat, Inflammation, Chronic 2 (40%) 1 (33%) 1 (50%) 2 (50%)
Fat, Necrosis 5 (100%) 2 (67%) 1 (50%) 2 (50%)
Pancreas (50) (33) (26) (48)
Hemorrhage 1 (2%)
Thrombosis 1 (4%)
Acinus, Atrophy 22 (44%) 10 (30%) 9 (35%) 15 (31%)
Acinus, Necrosis 1 (2%)
Pharynx (1) (1) (2) (4)
Palate, Cyst 1 (25%)
Palate, Developmental Malformation 2 (50%)
Palate, Inflammation 1 (50%)
Palate, Inflammation, Chronic 1 (100%) 1 (25%)
Salivary Glands (49) (33) (27) (49)
Inflammation, Suppurative 12 (24%) 6 (18%) 5 (19%) 13 (27%)
Duct, Hyperplasia 15 (31%) 16 (48%) 9 (33%) 16 (33%)
Stomach, Forestomach (50) (33) (28) (50)
Inflammation, Chronic 13 (26%) 9 (27%) 3 (11%) 3 (6%)
Inflammation, Suppurative 1 (2%) 1 (4%) 1 (2%)
Mineralization 1 (4%)
Ulcer 12 (24%) 7 (21%) 2 (7%) 4 (8%)
Epithelium, Hyperplasia 13 (26%) 11 (33%) 4 (14%) 4 (8%)
Stomach, Glandular (50) (32) (28) (49)
Ectopic Tissue 1 (4%)
Hemorrhage 1 (3%) 2 (4%)
Infiltration Cellular, Eosinophil 1 (2%)
Inflammation, Chronic 1 (2%) 5 (16%) 1 (4%)
Inflammation, Suppurative 8 (16%) 4 (13%) 1 (2%)
Mineralization 4 (8%) 1 (4%) 1 (2%)
Pigmentation, Hemosiderin 3 (9%) 1 (4%)
Ulcer 8 (16%) 9 (28%) 2 (7%) 3 (6%)
Tooth (1)
Inflammation, Chronic 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (3) (1)
Mineralization 3 (100%) 1 (100%)
Heart (50) (35) (30) (50)
Cardiomyopathy 47 (94%) 34 (97%) 30 (100%) 48 (96%)
Mineralization 1 (2%) 1 (3%)
Atrium, Congestion 1 (3%) 1 (3%)
Atrium, Thrombosis 3 (6%) 1 (3%) 5 (17%) 3 (6%)
Ventricle, Thrombosis 1 (3%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Gland, Cortex (50) (50) (48) (49)
Degeneration, Fatty 22 (44%) 26 (52%) 21 (44%) 24 (49%)
Focal Cellular Change 3 (6%) 1 (2%) 2 (4%) 2 (4%)
Hematopoietic Cell Proliferation 6 (12%) 5 (10%) 4 (8%) 16 (33%)
Hyperplasia 4 (8%) 3 (6%) 2 (4%) 2 (4%)
Hypertrophy 1 (2%)
Necrosis 1 (2%) 1 (2%)
Adrenal Gland, Medulla (42) (46) (47) (38)
Hematopoietic Cell Proliferation 1 (3%)
Hyperplasia 15 (36%) 19 (41%) 19 (40%) 17 (45%)
Necrosis 1 (2%) 1 (2%)
Islets, Pancreatic (50) (36) (27) (48)
Hyperplasia 3 (6%) 1 (3%) 3 (6%)
Parathyroid Gland (42) (32) (27) (41)
Hyperplasia 2 (5%) 4 (13%) 3 (11%) 3 (7%)
Thrombosis 1 (4%)
Pituitary Gland (47) (43) (40) (47)
Pars Distalis, Angiectasis 1 (2%)
Pars Distalis, Cyst 1 (2%) 1 (2%) 3 (8%) 1 (2%)
Pars Distalis, Hemorrhage 1 (2%) 1 (3%) 1 (2%)
Pars Distalis, Hyperplasia 12 (26%) 5 (12%) 9 (23%) 12 (26%)
Pars Distalis, Inflammation, Suppurative 1 (2%)
Pars Intermedia, Hyperplasia 1 (2%)
Thyroid Gland (48) (49) (46) (48)
C-Cell, Hyperplasia 8 (17%) 5 (10%) 10 (22%) 9 (19%)
Follicular Cell, Hyperplasia 1 (2%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Penis (1) (1)
Inflammation, Suppurative 1 (100%)
Preputial Gland (50) (48) (46) (50)
Cyst 2 (4%) 1 (2%)
Hyperplasia 1 (2%)
Hyperplasia, Squamous 1 (2%)
Inflammation, Suppurative 9 (18%) 8 (17%) 14 (30%) 10 (20%)
Prostate (50) (34) (27) (50)
Hyperplasia 6 (12%) 1 (3%) 1 (4%) 10 (20%)
Inflammation, Suppurative 23 (46%) 21 (62%) 14 (52%) 18 (36%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 11
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Seminal Vesicle (7) (3) (6) (8)
Dilatation 2 (67%) 2 (33%) 1 (13%)
Inflammation, Suppurative 6 (86%) 1 (33%) 2 (33%) 6 (75%)
Testes (50) (47) (50) (50)
Atrophy 14 (28%) 14 (30%) 17 (34%) 14 (28%)
Necrosis 1 (2%)
Interstitial Cell, Hyperplasia 6 (12%) 10 (21%) 11 (22%) 6 (12%)
Perivascular, Inflammation 5 (10%) 2 (4%) 4 (8%) 6 (12%)
Tunic, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (33) (26) (49)
Depletion Cellular 1 (2%)
Hyperplasia, Neutrophil 1 (2%)
Myelofibrosis 2 (4%) 4 (12%) 2 (8%) 2 (4%)
Lymph Node (50) (35) (30) (49)
Hyperplasia, Plasma Cell 1 (2%)
Mediastinal, Congestion 1 (3%)
Mesenteric, Angiectasis 1 (3%)
Mesenteric, Inflammation, Focal,
Granulomatous 1 (2%) 1 (3%)
Mesenteric, Pigmentation, Hemosiderin 1 (2%)
Renal, Congestion 1 (2%)
Renal, Hyperplasia 2 (4%)
Lymph Node, Bronchial (50) (29) (25) (47)
Hyperplasia 5 (10%) 1 (4%) 8 (17%)
Inflammation, Focal, Granulomatous 1 (2%) 1 (2%)
Inflammation, Suppurative 1 (4%)
Pigmentation, Hemosiderin 1 (2%)
Lymph Node, Mandibular (47) (27) (28) (46)
Fibrosis 1 (4%)
Hyperplasia 21 (45%) 6 (22%) 1 (4%) 18 (39%)
Inflammation, Focal, Granulomatous 2 (4%)
Spleen (50) (49) (49) (50)
Ectopic Tissue 1 (2%)
Fibrosis 3 (6%) 6 (12%) 7 (14%) 10 (20%)
Hematopoietic Cell Proliferation 1 (2%) 1 (2%) 1 (2%) 7 (14%)
Inflammation, Granulomatous 1 (2%) 1 (2%) 1 (2%)
Metaplasia, Osseous 1 (2%)
Necrosis 3 (6%) 1 (2%) 1 (2%) 2 (4%)
Pigmentation, Hemosiderin 1 (2%)
Thrombosis 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 12
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (21) (10) (12) (24)
Galactocele 7 (33%) 6 (60%) 2 (17%) 8 (33%)
Hyperplasia 6 (29%) 1 (10%) 6 (50%) 8 (33%)
Inflammation, Chronic 2 (20%) 1 (4%)
Skin (49) (32) (30) (50)
Atrophy 1 (2%)
Cyst Epithelial Inclusion 1 (3%)
Inflammation, Suppurative 1 (2%)
Ulcer 1 (2%)
Epidermis, Hyperplasia 1 (3%)
Subcutaneous Tissue, Fibrosis 1 (3%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (33) (29) (50)
Fibrous Osteodystrophy 5 (10%) 2 (6%) 1 (3%) 2 (4%)
Periosteum, Proliferation 3 (6%) 1 (3%) 15 (30%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (33) (30) (50)
Hemorrhage 2 (4%) 2 (6%) 4 (13%) 3 (6%)
Hydrocephalus 1 (2%)
Inflammation, Suppurative 1 (2%)
Necrosis 1 (3%)
Meninges, Pigmentation, Hemosiderin 1 (2%)
Meninges, Thrombosis 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (49) (30) (25) (48)
Inflammation 1 (4%)
Inflammation, Suppurative 22 (45%) 19 (63%) 13 (52%) 22 (46%)
Metaplasia, Squamous 1 (4%) 3 (6%)
Epithelium, Hyperplasia 1 (2%) 1 (4%)
Lung (50) (49) (50) (50)
Congestion 1 (2%) 2 (4%) 2 (4%) 5 (10%)
Edema 1 (2%)
Hemorrhage 3 (6%) 1 (2%) 3 (6%) 5 (10%)
Inflammation, Chronic, Focal 13 (26%) 11 (22%) 14 (28%) 9 (18%)
Inflammation, Focal, Granulomatous 1 (2%)
Mineralization 2 (4%) 1 (2%)
Pigmentation, Hemosiderin 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 13
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Alveolar Epithelium, Hyperplasia 5 (10%) 5 (10%) 4 (8%) 7 (14%)
Alveolus, Infiltration Cellular, Histiocyte 3 (6%) 9 (18%) 6 (12%) 8 (16%)
Peribronchial, Infiltration Cellular,
Mononuclear Cell 1 (2%)
Perivascular, Infiltration Cellular,
Mononuclear Cell 18 (36%) 12 (24%) 19 (38%) 24 (48%)
Nose (50) (50) (49) (50)
Hemorrhage 1 (2%)
Hyperplasia, Adenomatous 1 (2%)
Inflammation 28 (56%) 19 (38%) 24 (49%) 48 (96%)
Inflammation, Suppurative 46 (92%) 45 (90%) 46 (94%) 47 (94%)
Thrombosis 11 (22%) 10 (20%) 9 (18%) 1 (2%)
Nasolacrimal Duct, Inflammation, Suppurative 17 (34%) 14 (28%) 14 (29%) 13 (26%)
Olfactory Epithelium, Degeneration 1 (2%) 4 (8%) 3 (6%) 27 (54%)
Olfactory Epithelium, Metaplasia 2 (4%) 4 (8%) 10 (20%) 13 (26%)
Olfactory Epithelium, Metaplasia, Squamous 1 (2%) 6 (12%)
Respiratory Epithelium, Hyperplasia 12 (24%) 11 (22%) 12 (24%) 48 (96%)
Respiratory Epithelium, Metaplasia, Squamous 4 (8%) 5 (10%) 6 (12%) 44 (88%)
Submucosa, Hyperplasia 3 (6%)
Vomeronasal Organ, Inflammation, Suppurative 3 (6%) 7 (14%) 6 (12%) 8 (16%)
Trachea (49) (29) (26) (47)
Inflammation, Suppurative 2 (4%) 3 (10%) 2 (8%) 5 (11%)
Epithelium, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (48) (6) (4) (49)
Inflammation, Chronic 1 (2%)
Synechia 1 (25%) 2 (4%)
Anterior Chamber, Inflammation, Suppurative 3 (6%) 1 (17%) 3 (6%)
Cornea, Degeneration 1 (2%) 2 (4%)
Cornea, Inflammation, Suppurative 2 (4%) 3 (50%) 4 (8%)
Cornea, Mineralization 2 (4%) 2 (4%)
Lens, Degeneration 3 (6%) 3 (50%) 2 (4%)
Lids, Inflammation, Suppurative 1 (2%)
Retina, Degeneration 2 (4%) 3 (50%) 2 (4%)
Harderian Gland (7) (1)
Inflammation, Suppurative 5 (71%)
Metaplasia, Squamous 1 (14%)
Acinus, Hyperplasia 1 (14%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 14
NTP Experiment-Test: 05096-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC 0-CHLOROBENZALMALONONITRILE (CS-2) Date: 09/13/94
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 01:59:04
____________________________________________________________________________________________________________________________________
FISCHER 344 RATS MALE CONTROL .075 MG/ 0.25 MG/ 0.75 MG/
M3 M3 M3
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (44) (39) (50)
Cyst 2 (5%)
Hematopoietic Cell Proliferation 1 (2%)
Hemorrhage 1 (3%)
Hydronephrosis 1 (2%)
Infarct 1 (2%)
Inflammation, Suppurative 2 (5%) 2 (5%) 2 (4%)
Mineralization 1 (2%) 1 (2%) 1 (3%)
Nephropathy 50 (100%) 43 (98%) 39 (100%) 50 (100%)
Pigmentation, Hemosiderin 1 (2%)
Papilla, Necrosis 1 (3%)
Pelvis, Epithelium, Hyperplasia 1 (2%) 2 (5%) 1 (2%)
Renal Tubule, Hyperplasia 1 (2%) 6 (14%) 1 (3%) 3 (6%)
Urinary Bladder (49) (31) (27) (49)
Calculus Gross Observation 1 (4%)
Calculus Micro Observation Only 1 (4%)
Hemorrhage 2 (7%)
Inflammation 1 (3%) 1 (2%)
Inflammation, Suppurative 1 (2%) 3 (10%) 4 (15%)
Transitional Epithelium, Hyperplasia 1 (2%) 2 (6%) 1 (4%) 2 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 15
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