TDMS Study 05122-06 Pathology Tables
INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
NICKEL SULFATE HEXAHYDRATE
NTP Experiment-Test: 05122-06 Report: PEIRPT03
Study Type: CHRONIC Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
Facility: Lovelace Inhalation Toxicology Research Institute
Chemical CAS #: 010101970
Lock Date: 04/19/94
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 20 60 20 60 20
Scheduled Sacrifice 19 20 20
Early Deaths
Moribund Sacrifice 19 1 11 11
Natural Death 7 10 4
Survivors
Moribund Sacrifice 1 1
Terminal Sacrifice 33 38 45
Animals Examined Microscopically 60 11 60 10 60 10
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (59) (6) (55) (5) (57) (5)
Concretion 1 (2%)
Dilatation 1 (2%)
Intestine Large, Rectum (56) (4) (57) (4) (55) (4)
Autolysis 1 (2%)
Intestine Large, Cecum (60) (6) (57) (5) (60) (5)
Autolysis 1 (2%)
Intestine Small, Duodenum (57) (6) (57) (5) (59) (5)
Autolysis 1 (2%)
Inflammation 1 (2%)
Intussusception 1 (2%)
Intestine Small, Jejunum (60) (6) (58) (5) (59) (5)
Autolysis 1 (2%)
Peyer's Patch, Hyperplasia, Lymphoid 1 (2%) 1 (2%)
Intestine Small, Ileum (60) (6) (58) (5) (58) (5)
Autolysis 1 (2%)
Liver (60) (6) (59) (5) (60) (5)
Autolysis 1 (2%)
Basophilic Focus 2 (3%) 2 (3%)
Congestion 1 (2%)
Fatty Change 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hemorrhage 1 (2%)
Hepatodiaphragmatic Nodule 1 (2%)
Infarct 2 (3%) 1 (2%)
Inflammation 1 (2%) 1 (2%)
Necrosis 2 (3%) 1 (2%) 2 (3%)
Necrosis, Diffuse 4 (7%) 4 (7%) 1 (2%)
Mesentery (7) (4) (4)
Inflammation 1 (14%) 1 (25%)
Inflammation, Chronic 1 (25%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Pancreas (59) (6) (58) (5) (60) (5)
Amyloid Deposition 1 (2%)
Infiltration Cellular, Lymphocyte 1 (2%)
Inflammation 1 (2%)
Acinus, Hyperplasia, Focal 1 (2%)
Duct, Cyst, Multiple 1 (2%)
Salivary Glands (60) (6) (59) (5) (60) (5)
Angiectasis 1 (2%)
Stomach, Forestomach (60) (6) (58) (5) (60) (5)
Hyperplasia, Squamous 1 (2%) 2 (3%)
Inflammation 1 (2%) 2 (3%)
Stomach, Glandular (59) (6) (58) (5) (59) (5)
Hyperplasia 1 (2%)
Tooth (2) (1)
Peridontal Tissue, Inflammation 2 (100%) 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (60) (6) (59) (5) (60) (5)
Cardiomyopathy 1 (2%)
Infiltration Cellular, Lymphocyte 1 (2%)
Inflammation 1 (2%) 2 (3%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (59) (6) (58) (5) (60) (5)
Cyst 1 (2%)
Capsule, Hyperplasia 1 (2%)
Extra Adrenal Tissue, Inflammation 1 (2%)
Adrenal Medulla (59) (6) (57) (5) (60) (5)
Mineralization 1 (2%)
Parathyroid Gland (40) (4) (44) (3) (50) (5)
Cyst 1 (33%) 1 (2%)
Bilateral, Hyperplasia 1 (3%)
Pituitary Gland (58) (6) (56) (5) (59) (5)
Angiectasis 1 (2%) 1 (2%)
Cyst 1 (2%)
Pars Distalis, Angiectasis 3 (5%) 2 (4%) 8 (14%)
Pars Distalis, Hyperplasia 12 (21%) 8 (14%) 15 (25%)
Pars Distalis, Hyperplasia, Focal 1 (20%)
Thyroid Gland (59) (6) (59) (5) (60) (5)
Cyst 1 (17%)
Follicular Cell, Hyperplasia, Cystic 22 (37%) 20 (34%) 27 (45%)
Follicular Cell, Hyperplasia, Focal 1 (20%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Tissue NOS (5) (8) (4)
Thrombosis 1 (20%)
Abdominal, Cyst 1 (25%)
Abdominal, Inflammation 1 (13%)
Mediastinum, Infiltration Cellular,
Lymphocyte 1 (13%)
Mediastinum, Inflammation 1 (20%) 1 (13%)
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (57) (6) (56) (5) (52) (5)
Atrophy 1 (2%)
Ectasia 4 (7%) 4 (7%) 5 (10%)
Hyperplasia, Squamous 1 (2%)
Inflammation 2 (4%) 1 (17%) 4 (7%) 3 (6%)
Pigmentation 3 (5%) 2 (4%)
Ovary (58) (6) (58) (5) (60) (5)
Atrophy 1 (2%)
Cyst 6 (10%) 8 (14%) 17 (28%) 1 (20%)
Hemorrhage 1 (2%)
Inflammation 1 (2%)
Bilateral, Cyst 1 (2%) 1 (2%)
Periovarian Tissue, Cyst 1 (20%)
Uterus (60) (7) (60) (7) (60) (6)
Congestion 1 (2%)
Dilatation 1 (2%)
Hemorrhage 1 (2%)
Infiltration Cellular, Lymphocyte 1 (2%)
Inflammation 1 (2%)
Thrombosis 1 (2%)
Endometrium, Hyperplasia 30 (50%) 5 (71%) 25 (42%) 6 (86%) 39 (65%) 5 (83%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (60) (6) (59) (5) (60) (5)
Hyperplasia, Histiocytic 1 (2%) 1 (2%)
Hyperplasia, Megakaryocyte 1 (2%)
Hyperplasia, Plasma Cell 1 (2%)
Myelofibrosis 39 (65%) 43 (73%) 52 (87%)
Erythroid Cell, Hyperplasia 1 (2%)
Myeloid Cell, Hyperplasia 6 (10%) 4 (7%) 2 (3%)
Lymph Node (11) (11) (13)
Iliac, Hyperplasia, Lymphoid 1 (9%) 3 (27%) 1 (8%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Iliac, Hyperplasia, Plasma Cell 1 (9%)
Inguinal, Hyperplasia, Lymphoid 2 (18%) 2 (15%)
Pancreatic, Hyperplasia, Lymphoid 1 (9%) 1 (8%)
Pancreatic, Inflammation 1 (9%)
Renal, Hyperplasia, Lymphoid 1 (9%) 2 (18%)
Renal, Hyperplasia, Plasma Cell 1 (9%)
Lymph Node, Bronchial (50) (7) (54) (9) (58) (9)
Amyloid Deposition 1 (2%)
Congestion 1 (2%) 1 (2%)
Edema 1 (2%)
Hyperplasia, Lymphoid 15 (30%) 2 (29%) 9 (17%) 3 (33%) 16 (28%) 3 (33%)
Hyperplasia, Macrophage 2 (4%) 14 (24%)
Hyperplasia, Plasma Cell 2 (4%)
Inflammation 1 (2%)
Lymph Node, Mandibular (55) (9) (56) (7) (60) (6)
Congestion 1 (2%)
Cyst 1 (2%) 1 (2%)
Hyperplasia, Histiocytic 2 (4%)
Hyperplasia, Lymphoid 15 (27%) 6 (67%) 6 (11%) 2 (29%) 5 (8%) 2 (33%)
Hyperplasia, Mast Cell 1 (2%)
Hyperplasia, Plasma Cell 5 (9%) 5 (9%) 5 (8%)
Lymph Node, Mesenteric (56) (6) (52) (3) (56) (5)
Congestion 4 (7%) 3 (6%) 1 (2%)
Edema 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%) 4 (7%)
Hyperplasia, Plasma Cell 1 (2%)
Inflammation 1 (2%)
Lymph Node, Mediastinal (30) (2) (28) (5) (33) (1)
Hyperplasia, Histiocytic 2 (6%)
Hyperplasia, Lymphoid 3 (10%) 1 (3%)
Hyperplasia, Plasma Cell 1 (4%)
Inflammation 1 (3%) 1 (4%)
Spleen (60) (6) (58) (5) (60) (5)
Angiectasis 1 (2%)
Congestion 3 (5%) 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 11 (18%) 9 (16%) 8 (13%)
Hyperplasia, Lymphoid 10 (17%) 4 (7%) 9 (15%) 1 (20%)
Necrosis 1 (2%)
Thymus (57) (6) (54) (3) (54) (5)
Atrophy 14 (25%) 1 (17%) 11 (20%) 8 (15%)
Cyst 1 (17%) 1 (20%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (60) (6) (58) (5) (60) (5)
Ectasia 1 (2%) 2 (3%)
Hyperplasia 1 (2%)
Skin (60) (6) (59) (5) (59) (5)
Inflammation 1 (2%) 1 (17%) 1 (2%) 1 (2%)
Ulcer 1 (17%)
Dermis, Fibrosis 1 (2%) 1 (2%)
Dermis, Inflammation, Granulomatous 1 (2%)
Hindlimb, Hemorrhage 1 (2%)
Neck, Inflammation 1 (2%)
Pinna, Inflammation 1 (2%) 1 (2%)
Subcutaneous Tissue, Edema 1 (2%)
Subcutaneous Tissue, Inflammation 1 (20%)
Tail, Inflammation 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (60) (6) (60) (5) (60) (5)
Femur, Hyperostosis 1 (2%) 1 (2%)
Fibula, Fracture 1 (2%)
Periosteum, Femur, Inflammation 1 (2%)
Tibia, Fracture 1 (2%)
Skeletal Muscle (4) (3) (3)
Inflammation 1 (25%) 1 (33%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (60) (6) (59) (5) (59) (5)
Compression 2 (3%) 5 (8%) 3 (5%)
Cerebrum, Degeneration 3 (5%)
Meninges, Inflammation 1 (2%)
Pons, Degeneration 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (58) (4) (56) (4) (58) (2)
Hyperplasia 2 (3%)
Inflammation 1 (2%) 1 (2%) 1 (25%) 1 (2%)
Lung (60) (11) (60) (10) (60) (10)
Congestion 1 (2%) 1 (2%)
Emphysema 1 (2%)
Hemorrhage 4 (7%) 1 (2%) 3 (5%)
Hyperplasia, Macrophage 7 (12%) 24 (40%) 1 (10%) 53 (88%) 3 (30%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Inflammation 1 (2%)
Inflammation, Chronic Active 1 (2%) 7 (12%) 14 (23%)
Mineralization 1 (2%)
Alveolar Epithelium, Hyperplasia 9 (15%) 32 (53%) 1 (10%)
Alveolar Epithelium, Hyperplasia, Focal 1 (2%) 1 (10%) 1 (2%)
Alveolus, Proteinosis 11 (18%)
Interstitium, Infiltration Cellular 1 (9%) 4 (7%) 16 (27%)
Pleura, Inflammation 1 (2%)
Nose (60) (11) (59) (10) (60) (10)
Hemorrhage 1 (9%)
Inflammation, Acute 4 (36%) 4 (40%) 1 (10%)
Olfactory Epithelium, Atrophy 3 (5%) 2 (3%) 1 (2%)
Olfactory Epithelium, Degeneration 12 (20%) 1 (9%) 8 (14%) 1 (2%) 1 (10%)
Olfactory Epithelium, Inflammation 1 (2%)
Olfactory Epithelium, Respiratory Epithelium,
Degeneration 1 (9%) 1 (10%)
Respiratory Epithelium, Degeneration 11 (18%) 1 (9%) 14 (24%) 2 (20%) 4 (7%) 2 (20%)
Respiratory Epithelium, Inflammation 5 (8%) 7 (12%) 11 (18%)
Respiratory Epithelium, Metaplasia, Squamous 1 (2%) 3 (5%)
Squamous Epithelium, Inflammation 1 (2%)
Vomeronasal Organ, Infiltration Cellular,
Polymorphonuclear 1 (9%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Ear (1) (2)
Hemorrhage 1 (100%)
External Ear, Inflammation 2 (100%)
Lacrimal Gland (1)
Extraorbital, Hyperplasia, Plasma Cell 1 (100%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (60) (11) (60) (10) (60) (10)
Inflammation 1 (2%) 1 (2%)
Metaplasia, Osseous 1 (2%) 1 (2%)
Mineralization 2 (3%)
Nephropathy 3 (5%) 1 (2%) 1 (2%)
Glomerulus, Inflammation 1 (2%)
Medulla, Cyst 1 (2%)
Perirenal Tissue, Inflammation 1 (2%)
Renal Tubule, Necrosis, Acute 1 (2%)
Urinary Bladder (59) (5) (60) (5) (58) (5)
Angiectasis 1 (2%)
Autolysis 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Calculus Micro Observation Only 1 (2%)
Hemorrhage 1 (2%)
Infiltration Cellular, Lymphocyte 1 (2%)
Inflammation 1 (2%)
Transitional Epithelium, Hyperplasia 1 (2%) 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 20
Scheduled Sacrifice 20
Early Deaths
Moribund Sacrifice 17
Natural Death 6
Survivors
Terminal Sacrifice 37
Animals Examined Microscopically 60 10
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Liver (60) (5)
Eosinophilic Focus 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Infarct 1 (2%)
Necrosis 2 (3%)
Necrosis, Diffuse 6 (10%)
Bile Duct, Degeneration 1 (2%)
Mesentery (5)
Infiltration Cellular, Lymphocyte 1 (20%)
Inflammation 1 (20%)
Necrosis 1 (20%)
Pancreas (60) (5)
Inflammation 1 (2%)
Duct, Ectasia 1 (2%)
Stomach, Forestomach (60) (6)
Diverticulum 1 (17%)
Tooth (1)
Peridontal Tissue, Inflammation 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (60) (5)
Inflammation 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (60) (5)
Cyst 1 (2%)
Hyperplasia 1 (2%)
Hypertrophy 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Extra Adrenal Tissue, Inflammation 1 (2%)
Adrenal Medulla (60) (5)
Hyperplasia 1 (2%)
Parathyroid Gland (49) (3)
Hyperplasia 1 (2%)
Pituitary Gland (57) (4)
Congestion 1 (2%)
Pars Distalis, Angiectasis 1 (2%)
Pars Distalis, Hyperplasia 11 (19%)
Pars Distalis, Hyperplasia, Focal 2 (50%)
Thyroid Gland (60) (5)
Inflammation 2 (3%)
Follicular Cell, Hyperplasia, Cystic 27 (45%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Tissue NOS (3)
Abdominal, Inflammation 1 (33%)
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (58) (5)
Ectasia 2 (3%) 1 (20%)
Inflammation 1 (2%)
Ovary (59) (5)
Angiectasis 1 (2%)
Atrophy 1 (2%)
Cyst 8 (14%) 1 (20%)
Hemorrhage 2 (3%)
Inflammation 1 (2%)
Pigmentation, Hemosiderin 1 (2%)
Uterus (60) (6)
Hemorrhage 1 (2%)
Inflammation 1 (2%)
Prolapse 1 (2%)
Endometrium, Hyperplasia 41 (68%) 5 (83%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (60) (5)
Hyperplasia, Histiocytic 1 (2%)
Myelofibrosis 41 (68%)
Myeloid Cell, Hyperplasia 3 (5%)
Lymph Node (13)
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Iliac, Hyperplasia, Lymphoid 1 (8%)
Iliac, Hyperplasia, Plasma Cell 2 (15%)
Inguinal, Hyperplasia, Histiocytic 1 (8%)
Inguinal, Hyperplasia, Lymphoid 1 (8%)
Inguinal, Pigmentation 1 (8%)
Pancreatic, Hematopoietic Cell Proliferation 1 (8%)
Pancreatic, Inflammation 1 (8%)
Renal, Fibrosis 1 (8%)
Renal, Hyperplasia, Histiocytic 1 (8%)
Renal, Hyperplasia, Lymphoid 2 (15%)
Lymph Node, Bronchial (56) (8)
Hemorrhage 1 (2%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 26 (46%) 7 (88%)
Hyperplasia, Macrophage 37 (66%) 4 (50%)
Lymph Node, Mandibular (55) (6)
Cyst 1 (2%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 5 (9%) 1 (17%)
Hyperplasia, Plasma Cell 4 (7%) 1 (17%)
Inflammation 1 (2%)
Lymph Node, Mesenteric (51) (4)
Hemorrhage 1 (2%)
Hyperplasia, Lymphoid 2 (4%)
Inflammation 2 (4%)
Lymph Node, Mediastinal (22) (2)
Hyperplasia, Lymphoid 3 (14%)
Spleen (60) (5)
Hematopoietic Cell Proliferation 9 (15%)
Hyperplasia, Lymphoid 10 (17%)
Hyperplasia, Plasma Cell 1 (2%)
Thymus (56) (5)
Atrophy 12 (21%)
Autolysis 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (60) (5)
Hyperplasia 1 (2%)
Skin (60) (5)
Inflammation 3 (5%)
Pinna, Inflammation 1 (2%)
Subcutaneous Tissue, Edema 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 11
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
None
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (60) (5)
Compression 2 (3%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (56) (4)
Degeneration 1 (2%)
Hyperplasia 1 (2%)
Inflammation 2 (4%)
Lung (60) (10)
Congestion 1 (2%)
Hyperplasia, Macrophage 59 (98%) 10 (100%)
Inflammation, Chronic Active 40 (67%) 7 (70%)
Alveolar Epithelium, Hyperplasia 45 (75%) 5 (50%)
Alveolar Epithelium, Metaplasia, Squamous 1 (2%)
Alveolus, Proteinosis 45 (75%) 5 (50%)
Bronchiole, Degeneration 2 (3%)
Interstitium, Infiltration Cellular 39 (65%) 6 (60%)
Nose (60) (10)
Inflammation, Acute 5 (50%)
Olfactory Epithelium, Atrophy 17 (28%) 1 (10%)
Olfactory Epithelium, Degeneration 8 (13%)
Olfactory Epithelium, Inflammation 2 (3%)
Respiratory Epithelium, Degeneration 7 (12%) 2 (20%)
Respiratory Epithelium, Inflammation 11 (18%)
Respiratory Epithelium, Metaplasia, Squamous 2 (3%)
Squamous Epithelium, Hyperplasia 1 (2%)
Squamous Epithelium, Inflammation 1 (2%)
Trachea (59) (5)
Degeneration 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 12
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (60) (10)
Fibrosis 1 (2%)
Nephropathy 3 (5%)
Pelvis, Dilatation 2 (3%)
Perirenal Tissue, Inflammation 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 13
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 20 60 20 60 20
Scheduled Sacrifice 19 19 18
Early Deaths
Moribund Sacrifice 29 1 29 28 1
Natural Death 5 8 1 8 1
Survivors
Terminal Sacrifice 26 23 24
Animals Examined Microscopically 60 11 60 11 60 12
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (59) (5) (60) (6) (59) (7)
Hyperkeratosis 1 (2%) 1 (20%) 1 (2%)
Gallbladder (55) (6) (58) (6) (54) (7)
Autolysis 3 (5%) 1 (2%)
Intestine Large, Colon (59) (6) (60) (6) (58) (7)
Autolysis 1 (2%)
Hemorrhage 1 (2%)
Intestine Large, Rectum (54) (5) (56) (5) (49) (5)
Autolysis 1 (2%)
Anus, Inflammation 1 (2%)
Intestine Large, Cecum (58) (6) (59) (5) (58) (7)
Autolysis 1 (2%) 1 (2%) 1 (14%)
Hyperplasia, Lymphoid 1 (2%)
Intestine Small, Duodenum (60) (6) (59) (5) (57) (7)
Autolysis 1 (2%)
Intestine Small, Jejunum (60) (6) (60) (6) (57) (7)
Autolysis 1 (2%)
Peyer's Patch, Hyperplasia 1 (2%) 1 (2%)
Peyer's Patch, Inflammation 1 (2%)
Intestine Small, Ileum (58) (6) (59) (5) (55) (7)
Autolysis 1 (2%)
Liver (60) (6) (60) (7) (60) (8)
Angiectasis, Focal 1 (2%)
Autolysis 2 (3%)
Basophilic Focus 2 (3%) 4 (7%) 1 (2%)
Clear Cell Focus 1 (2%)
Congestion 1 (2%) 1 (2%)
Cyst 1 (2%)
Eosinophilic Focus 1 (2%)
Fatty Change 1 (2%)
Infarct 5 (8%) 1 (2%) 1 (2%)
Infarct, Focal 1 (14%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 14
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Inflammation 1 (2%) 1 (2%)
Necrosis 1 (2%) 1 (2%)
Necrosis, Diffuse 7 (12%) 5 (8%) 7 (12%)
Pigmentation 1 (2%)
Centrilobular, Degeneration 1 (2%) 1 (2%)
Hepatocyte, Hyperplasia 1 (17%) 1 (14%) 1 (13%)
Mesentery (1) (2) (1)
Inflammation 1 (100%)
Pancreas (60) (6) (59) (6) (58) (7)
Atrophy 1 (2%)
Autolysis 1 (2%)
Cyst 1 (2%)
Inflammation 1 (2%)
Acinus, Hyperplasia 1 (2%)
Pharynx (1)
Hyperkeratosis 1 (100%)
Salivary Glands (60) (6) (60) (6) (60) (7)
Autolysis 1 (2%)
Stomach, Forestomach (60) (6) (59) (5) (60) (7)
Autolysis 1 (2%)
Hyperplasia, Squamous 1 (2%)
Stomach, Glandular (60) (6) (59) (6) (58) (7)
Autolysis 1 (17%) 2 (3%)
Inflammation 1 (2%)
Tooth (4)
Peridontal Tissue, Inflammation 4 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (60) (6) (60) (6) (60) (7)
Cardiomyopathy 4 (7%) 1 (17%) 2 (3%) 3 (5%)
Inflammation 1 (2%) 1 (2%)
Atrium, Thrombosis 2 (3%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (60) (6) (59) (6) (59) (7)
Accessory Adrenal Cortical Nodule 1 (2%)
Cyst Multilocular 1 (2%)
Hyperplasia 2 (3%) 3 (5%)
Hypertrophy 1 (2%) 1 (2%)
Infarct 1 (2%)
Capsule, Hyperplasia 1 (2%)
Adrenal Medulla (60) (6) (57) (5) (57) (7)
Infarct 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 15
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Islets, Pancreatic (60) (6) (59) (6) (55) (7)
Hyperplasia 2 (4%)
Parathyroid Gland (48) (4) (41) (4) (49) (6)
Autolysis 1 (2%)
Pituitary Gland (52) (6) (53) (4) (56) (7)
Autolysis 1 (2%)
Cyst 1 (2%)
Pars Distalis, Hyperplasia 1 (2%) 2 (4%)
Thyroid Gland (60) (6) (60) (6) (59) (7)
Autolysis 1 (2%)
Follicular Cell, Hyperplasia 1 (2%)
Follicular Cell, Hyperplasia, Cystic 26 (43%) 26 (43%) 25 (42%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
Tissue NOS (1) (2) (1)
Abdominal, Abscess 1 (50%)
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (60) (6) (60) (6) (60) (7)
Autolysis 1 (2%)
Penis (4) (7) (9)
Hyperplasia, Squamous 4 (100%) 2 (29%) 6 (67%)
Inflammation 2 (29%) 3 (33%)
Preputial Gland (58) (8) (58) (6) (58) (9)
Atrophy 1 (2%) 2 (3%)
Cyst 1 (2%)
Ectasia 44 (76%) 8 (100%) 31 (53%) 5 (83%) 31 (53%) 8 (89%)
Inflammation 23 (40%) 2 (25%) 35 (60%) 2 (33%) 33 (57%) 1 (11%)
Duct, Hyperplasia 1 (2%)
Prostate (59) (5) (59) (6) (59) (6)
Autolysis 1 (2%)
Congestion 1 (2%)
Hyperplasia 1 (2%)
Inflammation 3 (5%) 3 (5%) 3 (5%)
Seminal Vesicle (60) (6) (60) (6) (59) (7)
Ectasia 1 (2%) 1 (2%) 1 (2%)
Hemorrhage 1 (2%)
Hyperplasia 1 (2%)
Testes (60) (6) (60) (6) (60) (7)
Atrophy 1 (2%) 1 (2%)
Autolysis 1 (2%)
Granuloma Sperm 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 16
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (60) (6) (60) (6) (59) (7)
Erythroid Cell, Hyperplasia 1 (2%)
Myeloid Cell, Hyperplasia 17 (28%) 1 (17%) 15 (25%) 11 (19%) 1 (14%)
Lymph Node (23) (1) (21) (1) (20) (2)
Iliac, Hyperplasia, Histiocytic 1 (4%)
Iliac, Hyperplasia, Lymphoid 4 (17%) 1 (5%) 1 (100%) 4 (20%) 2 (100%)
Iliac, Hyperplasia, Plasma Cell 12 (52%) 10 (48%) 7 (35%)
Iliac, Inflammation 1 (5%)
Iliac, Pigmentation 1 (4%)
Inguinal, Hyperplasia, Histiocytic 2 (10%)
Inguinal, Hyperplasia, Lymphoid 8 (35%) 11 (52%) 1 (100%) 7 (35%)
Inguinal, Hyperplasia, Plasma Cell 6 (26%) 5 (24%) 4 (20%)
Inguinal, Inflammation 1 (4%) 2 (10%) 1 (5%)
Inguinal, Pigmentation 2 (9%) 1 (5%)
Lumbar, Hyperplasia, Lymphoid 1 (50%)
Lumbar, Hyperplasia, Plasma Cell 1 (4%) 1 (5%)
Pancreatic, Thrombosis 1 (5%)
Popliteal, Hyperplasia, Plasma Cell 1 (4%)
Renal, Congestion 1 (5%)
Renal, Hyperplasia, Histiocytic 1 (5%)
Renal, Hyperplasia, Lymphoid 1 (4%)
Renal, Hyperplasia, Plasma Cell 4 (17%) 1 (100%) 1 (5%) 1 (5%)
Renal, Inflammation 2 (9%) 1 (5%)
Lymph Node, Bronchial (46) (9) (48) (9) (44) (8)
Congestion 1 (2%) 1 (2%)
Edema 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hyperplasia, Histiocytic 2 (4%)
Hyperplasia, Lymphoid 2 (4%) 2 (22%) 4 (8%) 4 (44%) 2 (5%) 2 (25%)
Hyperplasia, Macrophage 1 (11%) 8 (18%)
Hyperplasia, Plasma Cell 1 (2%) 2 (4%)
Lymph Node, Mandibular (55) (5) (49) (6) (55) (8)
Ectasia 1 (2%)
Hyperplasia, Lymphoid 2 (4%) 2 (4%) 4 (7%)
Hyperplasia, Plasma Cell 1 (2%) 3 (6%)
Inflammation 1 (2%) 1 (17%)
Lymph Node, Mesenteric (55) (5) (53) (5) (55) (5)
Angiectasis 2 (4%)
Congestion 2 (4%) 4 (8%) 1 (2%)
Hematopoietic Cell Proliferation 2 (4%)
Hyperplasia, Histiocytic 2 (4%) 2 (4%)
Hyperplasia, Lymphoid 4 (8%) 2 (4%)
Inflammation 5 (9%) 5 (9%)
Lymph Node, Mediastinal (18) (24) (7) (20)
Hyperplasia, Histiocytic 2 (8%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 17
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Hyperplasia, Lymphoid 1 (6%) 1 (14%)
Hyperplasia, Plasma Cell 1 (6%)
Pigmentation 1 (4%)
Spleen (60) (6) (60) (6) (59) (7)
Angiectasis 1 (2%)
Autolysis 1 (2%)
Hematopoietic Cell Proliferation 9 (15%) 12 (20%) 11 (19%)
Hyperplasia, Histiocytic 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 3 (5%) 1 (2%)
Inflammation 2 (3%)
Thymus (54) (5) (48) (6) (51) (7)
Atrophy 27 (50%) 1 (20%) 14 (29%) 1 (17%) 17 (33%) 2 (29%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (60) (6) (60) (7) (60) (7)
Hyperkeratosis 1 (17%)
Hyperplasia, Squamous 1 (2%)
Inflammation 4 (7%) 1 (17%) 1 (2%) 4 (7%)
Inflammation, Chronic 1 (2%)
Necrosis 1 (2%)
Dermis, Inflammation, Chronic Active 1 (14%)
Foot, Inflammation 1 (2%) 1 (2%) 2 (3%) 1 (14%)
Foot, Necrosis 1 (2%)
Pinna, Inflammation 1 (2%)
Prepuce, Edema 1 (2%)
Prepuce, Hyperplasia, Squamous 1 (2%)
Prepuce, Inflammation 8 (13%) 15 (25%) 18 (30%) 1 (14%)
Subcutaneous Tissue, Inflammation 1 (2%) 1 (17%)
Tail, Hyperkeratosis 1 (2%) 1 (2%)
Tail, Inflammation 5 (8%) 2 (3%) 1 (2%) 1 (14%)
Tail, Necrosis 11 (18%) 4 (7%) 5 (8%) 1 (14%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (60) (6) (60) (6) (60) (7)
Femur, Hyperostosis 2 (3%) 1 (2%) 2 (3%)
Femur, Osteoporosis 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 18
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (60) (6) (60) (6) (60) (7)
Cerebrum, Degeneration 1 (2%)
Hippocampus, Degeneration 1 (2%)
Meninges, Inflammation 1 (2%)
Thalamus, Degeneration 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (59) (5) (58) (6) (56) (6)
Autolysis 1 (2%)
Hyperplasia, Squamous 1 (2%)
Inflammation 1 (20%)
Lung (60) (11) (60) (11) (60) (12)
Autolysis 1 (2%)
Congestion 2 (3%)
Fibrosis 1 (2%) 1 (2%)
Hemorrhage 1 (2%)
Hyperplasia, Macrophage 6 (10%) 9 (15%) 1 (9%) 35 (58%) 5 (42%)
Infarct 1 (2%)
Inflammation 1 (2%) 2 (3%)
Inflammation, Acute 1 (9%)
Inflammation, Chronic Active 1 (2%) 2 (3%) 8 (13%)
Alveolar Epithelium, Hyperplasia 1 (2%) 4 (7%) 1 (9%) 19 (32%)
Alveolar Epithelium, Hyperplasia, Focal 1 (9%)
Bronchiole, Hyperplasia 1 (2%)
Interstitium, Infiltration Cellular 1 (2%) 3 (5%)
Interstitium, Inflammation, Focal 1 (9%)
Nose (60) (11) (60) (11) (59) (12)
Developmental Malformation 1 (9%)
Inflammation, Acute 2 (18%) 2 (18%) 1 (8%)
Glands, Inflammation 1 (9%)
Olfactory Epithelium, Atrophy 12 (20%) 1 (8%)
Olfactory Epithelium, Degeneration 2 (18%) 1 (2%)
Olfactory Epithelium, Inflammation 1 (2%)
Respiratory Epithelium, Degeneration 2 (3%) 2 (18%) 2 (3%)
Respiratory Epithelium, Inflammation 2 (3%) 2 (3%) 5 (8%)
Respiratory Epithelium, Metaplasia, Squamous 1 (2%) 1 (2%)
Vomeronasal Organ, Infiltration Cellular,
Polymorphonuclear 1 (9%) 1 (9%)
Trachea (60) (6) (60) (6) (59) (7)
Autolysis 1 (2%)
Inflammation 1 (14%)
Epithelium, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 19
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 0.0 0.0MG/M3 0.25 .25MG/M3 0.5 0.5MG/M3
MG/M3 LUNG TOX MG/M3 LUNG TOX MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Eye (1)
Phthisis Bulbi 1 (100%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (60) (11) (60) (11) (59) (12)
Congestion 1 (2%)
Dilatation 1 (2%)
Fibrosis 1 (2%)
Hydronephrosis 2 (3%)
Inflammation 8 (13%) 7 (12%) 8 (14%)
Mineralization 1 (2%)
Nephropathy 9 (15%) 8 (13%) 9 (15%)
Cortex, Cyst 2 (3%) 1 (2%)
Pelvis, Dilatation 16 (27%) 19 (32%) 9 (15%) 1 (8%)
Renal Tubule, Pigmentation 1 (2%)
Ureter (2) (1)
Dilatation 1 (50%)
Inflammation 1 (50%) 1 (100%)
Urethra (10) (12) (1) (15)
Calculus Micro Observation Only 10 (100%) 7 (58%) 1 (100%) 15 (100%)
Inflammation 1 (10%) 4 (33%) 3 (20%)
Transitional Epithelium, Hyperplasia 2 (17%)
Urinary Bladder (59) (6) (59) (6) (58) (7)
Autolysis 1 (2%)
Calculus Micro Observation Only 1 (2%) 2 (3%) 1 (2%)
Congestion 1 (14%)
Dilatation 1 (2%) 1 (14%)
Hemorrhage 1 (14%)
Inflammation 7 (12%) 6 (10%) 9 (16%)
Transitional Epithelium, Hyperplasia 1 (2%) 1 (2%) 1 (2%)
Transitional Epithelium, Metaplasia, Squamous 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 20
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 20
Scheduled Sacrifice 18
Early Deaths
Moribund Sacrifice 27
Natural Death 8 2
Survivors
Terminal Sacrifice 25
Animals Examined Microscopically 60 12
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Esophagus (59) (7)
Hyperkeratosis 1 (2%)
Gallbladder (53) (6)
Autolysis 1 (2%)
Intestine Large, Rectum (49) (5)
Autolysis 1 (2%)
Anus, Inflammation 1 (2%)
Intestine Large, Cecum (57) (6)
Autolysis 4 (7%)
Intestine Small, Duodenum (55) (5)
Autolysis 2 (4%)
Intestine Small, Jejunum (58) (6)
Autolysis 3 (5%)
Intestine Small, Ileum (55) (6)
Autolysis 3 (5%)
Liver (59) (6)
Clear Cell Focus 1 (2%)
Hepatodiaphragmatic Nodule 1 (2%)
Infarct 3 (5%)
Inflammation 1 (2%)
Necrosis 1 (2%) 1 (17%)
Necrosis, Diffuse 6 (10%)
Bile Duct, Cyst 2 (3%)
Mesentery (1)
Inflammation 1 (100%)
Pharynx (2)
Hyperplasia, Squamous 2 (100%)
Salivary Glands (60) (6)
Autolysis 1 (2%)
Stomach, Glandular (59) (6)
Autolysis 2 (3%)
Tooth (2)
a Number of animals examined microscopically at site and number of animals with lesion
Page 21
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Peridontal Tissue, Inflammation 2 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (60) (6)
Cardiomyopathy 2 (3%)
Inflammation 2 (3%)
Thrombosis 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (59) (6)
Hyperplasia 1 (2%)
Parathyroid Gland (43) (6)
Autolysis 1 (2%) 1 (17%)
Thyroid Gland (60) (7)
Autolysis 1 (14%)
Follicular Cell, Hyperplasia, Cystic 25 (42%)
Follicular Cell, Hyperplasia, Focal 1 (14%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (59) (7)
Autolysis 1 (2%)
Penis (13)
Bacterium 1 (8%)
Hyperplasia, Squamous 3 (23%)
Inflammation 8 (62%)
Preputial Gland (59) (7)
Atrophy 1 (2%)
Autolysis 1 (2%)
Ectasia 36 (61%) 7 (100%)
Inflammation 35 (59%) 3 (43%)
Duct, Hyperplasia 1 (2%)
Prostate (59) (7)
Autolysis 1 (2%)
Inflammation 1 (2%) 1 (14%)
Seminal Vesicle (59) (7)
Autolysis 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 22
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Ectasia 2 (3%)
Inflammation 1 (14%)
Testes (59) (7)
Autolysis 1 (2%) 1 (14%)
Tunic, Inflammation 2 (3%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (60) (6)
Autolysis 1 (2%)
Myeloid Cell, Hyperplasia 11 (18%) 1 (17%)
Lymph Node (25) (3)
Iliac, Hyperplasia, Histiocytic 1 (4%)
Iliac, Hyperplasia, Lymphoid 2 (67%)
Iliac, Hyperplasia, Plasma Cell 11 (44%) 1 (33%)
Iliac, Inflammation 1 (33%)
Inguinal, Hyperplasia, Histiocytic 2 (8%)
Inguinal, Hyperplasia, Lymphoid 11 (44%) 1 (33%)
Inguinal, Hyperplasia, Plasma Cell 3 (12%)
Inguinal, Inflammation 1 (4%)
Inguinal, Pigmentation 2 (8%)
Inguinal, Thrombosis 1 (33%)
Lumbar, Hyperplasia, Plasma Cell 2 (8%)
Pancreatic, Hyperplasia, Lymphoid 1 (4%)
Renal, Hyperplasia, Lymphoid 2 (8%) 1 (33%)
Lymph Node, Bronchial (53) (11)
Congestion 1 (2%)
Hyperplasia, Lymphoid 17 (32%)
Hyperplasia, Macrophage 38 (72%) 5 (45%)
Inflammation 1 (2%)
Lymph Node, Mandibular (44) (5)
Hyperplasia, Lymphoid 1 (2%)
Hyperplasia, Plasma Cell 1 (2%)
Inflammation 1 (2%)
Lymph Node, Mesenteric (53) (5)
Atrophy 1 (2%)
Congestion 4 (8%)
Edema 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hyperplasia, Lymphoid 3 (6%)
Inflammation 2 (4%)
Lymph Node, Mediastinal (23) (1)
Autolysis 1 (4%)
Congestion 1 (4%)
Hyperplasia, Histiocytic 1 (4%)
Hyperplasia, Lymphoid 1 (4%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 23
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Hyperplasia, Plasma Cell 1 (4%)
Spleen (59) (6)
Hematopoietic Cell Proliferation 10 (17%) 1 (17%)
Hyperplasia, Lymphoid 5 (8%)
Thymus (44) (6)
Atrophy 12 (27%) 1 (17%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (60) (6)
Inflammation 1 (2%)
Necrosis 1 (2%)
Ulcer, Chronic Active 1 (17%)
Foot, Inflammation 1 (2%)
Prepuce, Inflammation 12 (20%) 1 (17%)
Subcutaneous Tissue, Edema 1 (2%)
Tail, Inflammation 2 (3%)
Tail, Necrosis 6 (10%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
None
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (60) (7)
Autolysis 1 (14%)
Congestion 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (60) (5)
Autolysis 1 (20%)
Hyperplasia, Squamous 1 (2%)
Lung (60) (11)
Hemorrhage 1 (2%)
Hyperplasia, Macrophage 58 (97%) 11 (100%)
Inflammation, Chronic Active 28 (47%) 5 (45%)
Thrombosis, Multiple 1 (2%)
Alveolar Epithelium, Hyperplasia 39 (65%) 5 (45%)
Alveolus, Proteinosis 41 (68%) 4 (36%)
Interstitium, Infiltration Cellular 17 (28%) 6 (55%)
Nose (59) (11)
a Number of animals examined microscopically at site and number of animals with lesion
Page 24
NTP Experiment-Test: 05122-06 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC NICKEL SULFATE HEXAHYDRATE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 03:47:06
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE 1.0 1.0MG/M3
MG/M3 LUNG TOX
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM - CONT
Autolysis 1 (2%)
Inflammation, Acute 2 (18%)
Glands, Inflammation 1 (9%)
Olfactory Epithelium, Atrophy 36 (61%) 6 (55%)
Olfactory Epithelium, Inflammation 1 (9%)
Respiratory Epithelium, Degeneration 2 (3%)
Respiratory Epithelium, Inflammation 5 (8%)
Trachea (59) (7)
Autolysis 1 (14%)
Inflammation 1 (14%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (59) (12)
Autolysis 1 (8%)
Inflammation 6 (10%) 1 (8%)
Metaplasia, Osseous 1 (2%)
Mineralization 2 (3%)
Nephropathy 8 (14%)
Pelvis, Dilatation 10 (17%)
Ureter (1) (1)
Inflammation 1 (100%) 1 (100%)
Urethra (16)
Calculus Micro Observation Only 14 (88%)
Inflammation 5 (31%)
Urinary Bladder (58) (6)
Autolysis 3 (5%)
Calculus Micro Observation Only 2 (3%)
Dilatation 2 (3%)
Hemorrhage 1 (2%)
Inflammation 5 (9%) 1 (17%)
a Number of animals examined microscopically at site and number of animals with lesion
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