TDMS Study 05130-03 Pathology Tables
NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. I. DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 Facility: Microbiological Associates Chemical CAS #: 10401-500 Lock Date: 11/21/91 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Note: Animals arranged according to CID number Page 1 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. I. DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 5| 6| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 4| 7| 6| 7| 7| DAY ON TEST | 7| 3| 3| 7| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 7| 3| 2| 3| 6| 3| 3| | 3| 4| 2| 1| 2| 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 8| 4| 6| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 0 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Mesentery | + + + + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Acinus, Leukemia Mononuclear | X | __________________________________________________________________________| Pharynx | | Palate, Squamous Cell Papilloma | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + M + + + + + + + + +| Leukemia Mononuclear | X X X | Pheochromocytoma Benign | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + M M + + + + + M + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 2 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. I. DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 5| 6| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 4| 7| 6| 7| 7| DAY ON TEST | 7| 3| 3| 7| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 7| 3| 2| 3| 6| 3| 3| | 3| 4| 2| 1| 2| 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 8| 4| 6| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 0 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Leukemia Mononuclear | X X | Pars Distalis, Adenoma | X X X X X X X X X X X X| Pars Distalis, Adenoma, Multiple | X | Pars Distalis, Carcinoma | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | Bilateral, C-Cell, Adenoma | X | C-Cell, Adenoma | X | C-Cell, Carcinoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + M + + + + + + +| Adenoma | X | Leukemia Mononuclear | X X | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X X | Polyp Stromal | X | __________________________________________________________________________| Vagina | + | Leiomyosarcoma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Lymph Node | + + + | Bronchial, Leukemia Mononuclear | X | Iliac, Leukemia Mononuclear | X | Lumbar, Leukemia Mononuclear | | Mediastinal, Leukemia Mononuclear | | Pancreatic, Leukemia Mononuclear | X | Renal, Leukemia Mononuclear | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + M +| Leukemia Mononuclear | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Thymus | + + + + + + M + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| Page 3 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. I. DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 5| 6| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 4| 7| 6| 7| 7| DAY ON TEST | 7| 3| 3| 7| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 7| 3| 2| 3| 6| 3| 3| | 3| 4| 2| 1| 2| 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 8| 4| 6| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 0 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X X | Fibroadenoma | X X X X X X X X | Fibroadenoma, Multiple | X X X | Leukemia Mononuclear | X X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Pituitary | | Gland | X | Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Thyroid Gland | | Leukemia Mononuclear | X X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + M + + M + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + M + + M + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | _____________________________________________________________________________________________________________________| Page 4 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 3| 3| 8| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 6| 3| 3| 3| 3| 3| 1| 6| 3| | 4| 4| 4| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 4| 4| 4| 4| 4| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 0 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Colon | + + + A + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Rectum | + + + A + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Cecum | + + + A + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + A + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Jejunum | + + + A + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Ileum | + + + A + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Mesentery | + + + + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Acinus, Leukemia Mononuclear | X X | __________________________________________________________________________| Pharynx | + | Palate, Squamous Cell Papilloma | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Parathyroid Gland | + + + M + + + M + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 5 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. I. DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 3| 3| 8| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 6| 3| 3| 3| 3| 3| 1| 6| 3| | 4| 4| 4| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 4| 4| 4| 4| 4| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 0 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Leukemia Mononuclear | X X X | Pars Distalis, Adenoma | X X X X X X X X X X X X X X| Pars Distalis, Adenoma, Multiple | X | Pars Distalis, Carcinoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Bilateral, C-Cell, Adenoma | | C-Cell, Adenoma | X | C-Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + M + + + + + + + + + + + + M + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X X | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Leukemia Mononuclear | X X X | Polyp Stromal | | __________________________________________________________________________| Vagina | | Leiomyosarcoma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + M + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Lymph Node | + + + + | Bronchial, Leukemia Mononuclear | | Iliac, Leukemia Mononuclear | | Lumbar, Leukemia Mononuclear | X | Mediastinal, Leukemia Mononuclear | X X | Pancreatic, Leukemia Mononuclear | | Renal, Leukemia Mononuclear | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Thymus | + + + M + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| Page 6 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 3| 3| 8| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 6| 3| 3| 3| 3| 3| 1| 6| 3| | 4| 4| 4| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 4| 4| 4| 4| 4| 6| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 0 PPM | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Fibroadenoma | X X X X X X | Fibroadenoma, Multiple | X X | Leukemia Mononuclear | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Pituitary | | Gland | | Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Thyroid Gland | X | Leukemia Mononuclear | X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + M + + + M + + + + + + + + M +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | _____________________________________________________________________________________________________________________| Page 7 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 4| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 9| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | A | 0 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | | 50 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Intestine Large, Colon | | 49 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Large, Rectum | | 48 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Large, Cecum | | 48 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Small, Duodenum | | 48 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Small, Jejunum | | 48 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Small, Ileum | | 48 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Liver | | 50 | Leukemia Mononuclear | | 11 | __________________________________________________________________________|____________| Mesentery | | 8 | __________________________________________________________________________|____________| Pancreas | | 50 | Leukemia Mononuclear | | 2 | Acinus, Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Pharynx | | 1 | Palate, Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Salivary Glands | | 50 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Stomach, Forestomach | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Stomach, Glandular | | 50 | Leukemia Mononuclear | | 3 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | | 50 | Leukemia Mononuclear | | 6 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Adrenal Medulla | | 49 | Leukemia Mononuclear | | 5 | Pheochromocytoma Benign | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 8 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 4| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 9| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | A | 0 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Islets, Pancreatic | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Parathyroid Gland | | 45 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Pituitary Gland | | 50 | Leukemia Mononuclear | | 5 | Pars Distalis, Adenoma | | 26 | Pars Distalis, Adenoma, Multiple | | 2 | Pars Distalis, Carcinoma | | 2 | __________________________________________________________________________|____________| Thyroid Gland | | 50 | Leukemia Mononuclear | | 2 | Bilateral, C-Cell, Adenoma | | 1 | C-Cell, Adenoma | | 2 | C-Cell, Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | | 47 | Adenoma | | 2 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Ovary | | 50 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Uterus | | 50 | Adenoma | | 1 | Leukemia Mononuclear | | 5 | Polyp Stromal | | 1 | __________________________________________________________________________|____________| Vagina | | 1 | Leiomyosarcoma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | | 49 | Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Lymph Node | | 7 | Bronchial, Leukemia Mononuclear | | 1 | Iliac, Leukemia Mononuclear | | 1 | Lumbar, Leukemia Mononuclear | | 1 | Mediastinal, Leukemia Mononuclear | | 2 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 9 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 4| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 9| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | A | 0 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Pancreatic, Leukemia Mononuclear | | 1 | Renal, Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | | 49 | Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | | 50 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Spleen | | 50 | Leukemia Mononuclear | | 11 | __________________________________________________________________________|____________| Thymus | | 48 | Leukemia Mononuclear | | 6 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | | 50 | Adenocarcinoma | | 3 | Fibroadenoma | | 14 | Fibroadenoma, Multiple | | 5 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Skin | | 50 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | | 50 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | | 50 | Carcinoma, Metastatic, Pituitary | | | Gland | | 1 | Leukemia Mononuclear | | 3 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | | 50 | Carcinoma, Metastatic, Thyroid Gland | | 1 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Nose | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Trachea | | 50 | Leukemia Mononuclear | | 2 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 10 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 4| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 9| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | A | 0 PPM | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Eye | | 2 | __________________________________________________________________________|____________| Zymbal's Gland | | 45 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | | 50 | Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Urinary Bladder | | 48 | Leukemia Mononuclear | | 4 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | | 50 | Leukemia Mononuclear | | 11 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 11 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| 6| 4| 7| 6| 6| 5| 7| DAY ON TEST | 5| 3| 3| 3| 3| 5| 3| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 8| 1| 3| 4| 2| 2| 3| | 2| 3| 3| 3| 3| 9| 3| 3| 3| 7| 3| 0| 3| 3| 0| 3| 3| 3| 1| 9| 3| 9| 1| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 1000 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | Lipoma | X | __________________________________________________________________________| Intestine Large, Colon | A + + + + + + + + A + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Rectum | A + + + + + + + + A + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Large, Cecum | A + + + + + + + + A + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Duodenum | A + + + + + + + + A + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Small, Jejunum | A + + + + + + + + A + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Ileum | A + + + + + + + + A + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Mesentery | + + + | Sarcoma, Metastatic, Skin | X | Schwannoma Malignant, Metastatic, | | Uterus | X | Fat, Leukemia Mononuclear | | __________________________________________________________________________| Pancreas | A + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Leukemia Mononuclear | X X | __________________________________________________________________________| Pharynx | + | Palate, Squamous Cell Papilloma | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + M + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + M + + + + + +| Leukemia Mononuclear | X | Sarcoma, Metastatic, Skin | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Schwannoma NOS | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 12 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| 6| 4| 7| 6| 6| 5| 7| DAY ON TEST | 5| 3| 3| 3| 3| 5| 3| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 8| 1| 3| 4| 2| 2| 3| | 2| 3| 3| 3| 3| 9| 3| 3| 3| 7| 3| 0| 3| 3| 0| 3| 3| 3| 1| 9| 3| 9| 1| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 1000 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Leukemia Mononuclear | X X | __________________________________________________________________________| Islets, Pancreatic | A + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Parathyroid Gland | + M M + + + + + + + + + + M + + + + + + + + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + M + + + + + + + + +| Leukemia Mononuclear | X X X | Pars Distalis, Adenoma | X X X X X X X X X X X X X X X | Pars Distalis, Adenoma, Multiple | X | Pars Intermedia, Adenoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | C-Cell, Adenoma | X X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | + | Schwannoma Malignant, Metastatic, | | Uterus | X | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | | Leukemia Mononuclear | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Granulosa Cell Tumor Benign | | Leukemia Mononuclear | X X X X | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Leukemia Mononuclear | X X | Polyp Stromal | X X X X X X X X X| Sarcoma | X | Schwannoma Malignant | X | Cervix, Schwannoma Malignant, | | Metastatic, Uterus | X | __________________________________________________________________________| Vagina | + | Schwannoma Malignant, Metastatic, | | Uterus | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Lymph Node | + + + | Axillary, Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| Page 13 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| 6| 4| 7| 6| 6| 5| 7| DAY ON TEST | 5| 3| 3| 3| 3| 5| 3| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 8| 1| 3| 4| 2| 2| 3| | 2| 3| 3| 3| 3| 9| 3| 3| 3| 7| 3| 0| 3| 3| 0| 3| 3| 3| 1| 9| 3| 9| 1| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 1000 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Deep Cervical, Leukemia Mononuclear | | Iliac, Leukemia Mononuclear | | Mediastinal, Leukemia Mononuclear | X X | Pancreatic, Leukemia Mononuclear | X | Renal, Leukemia Mononuclear | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Sarcoma, Metastatic, Skin | X | Schwannoma Malignant, Metastatic, | | Uterus | X | __________________________________________________________________________| Spleen | A + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + M + + + + + + + + + +| Leukemia Mononuclear | X X | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X | Fibroadenoma | X X X X| Fibroadenoma, Multiple | X | Leukemia Mononuclear | | Sarcoma, Metastatic, Skin | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | X | Leukemia Mononuclear | | Subcutaneous Tissue, Fibrosarcoma | X | Subcutaneous Tissue, Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | | Leukemia Mononuclear | X X X X X | Sarcoma, Metastatic, Skin | X | Mediastinum, Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 14 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 4| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| 6| 4| 7| 6| 6| 5| 7| DAY ON TEST | 5| 3| 3| 3| 3| 5| 3| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 8| 1| 3| 4| 2| 2| 3| | 2| 3| 3| 3| 3| 9| 3| 3| 3| 7| 3| 0| 3| 3| 0| 3| 3| 3| 1| 9| 3| 9| 1| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 1000 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Lymphocytic | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + + | __________________________________________________________________________| Harderian Gland | + | __________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + M + + + + + + + M + + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Lipoma | X | __________________________________________________________________________| Urinary Bladder | + + + M + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | Lymphoma Malignant Lymphocytic | | _____________________________________________________________________________________________________________________| Page 15 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 6| 7| 4| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 5| 5| DAY ON TEST | 3| 3| 2| 3| 5| 3| 3| 3| 3| 3| 9| 2| 9| 1| 3| 8| 3| 5| 3| 3| 3| 8| 2| 7| 0| | 3| 3| 1| 3| 2| 3| 3| 3| 3| 3| 9| 6| 4| 3| 3| 4| 3| 9| 3| 3| 3| 4| 4| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 1000 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Lipoma | | __________________________________________________________________________| Intestine Large, Colon | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Large, Rectum | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Cecum | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + A + + + + + + + A + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Small, Ileum | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Mesentery | + + + | Sarcoma, Metastatic, Skin | | Schwannoma Malignant, Metastatic, | | Uterus | | Fat, Leukemia Mononuclear | X | __________________________________________________________________________| Pancreas | + + + + A + + + + + + + + + + + + + + + + + + + +| Acinus, Leukemia Mononuclear | X X X | __________________________________________________________________________| Pharynx | | Palate, Squamous Cell Papilloma | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Stomach, Glandular | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | Sarcoma, Metastatic, Skin | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | Schwannoma NOS | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 16 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 6| 7| 4| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 5| 5| DAY ON TEST | 3| 3| 2| 3| 5| 3| 3| 3| 3| 3| 9| 2| 9| 1| 3| 8| 3| 5| 3| 3| 3| 8| 2| 7| 0| | 3| 3| 1| 3| 2| 3| 3| 3| 3| 3| 9| 6| 4| 3| 3| 4| 3| 9| 3| 3| 3| 4| 4| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 1000 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Leukemia Mononuclear | X X X X | __________________________________________________________________________| Islets, Pancreatic | + + + + A + + + + + + + + + + + + + + + + + + + +| Adenoma | | Leukemia Mononuclear | X X | __________________________________________________________________________| Parathyroid Gland | + + + + M + + + + M + + + + + + + + + M M + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + M + + + + + + + + + M + + + + +| Leukemia Mononuclear | X X X X X X X | Pars Distalis, Adenoma | X X X X X X X X X X X X X X | Pars Distalis, Adenoma, Multiple | X| Pars Intermedia, Adenoma | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | C-Cell, Adenoma | X X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | __________________________________________________________________________| Tissue NOS | | Schwannoma Malignant, Metastatic, | | Uterus | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + + + + M + + + + + + + + + + + M + +| Carcinoma | X| Leukemia Mononuclear | X X | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Granulosa Cell Tumor Benign | X | Leukemia Mononuclear | X X X X X | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X X X | Polyp Stromal | X X X | Sarcoma | | Schwannoma Malignant | | Cervix, Schwannoma Malignant, | | Metastatic, Uterus | | __________________________________________________________________________| Vagina | | Schwannoma Malignant, Metastatic, | | Uterus | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Lymph Node | + + + + + + + + +| Axillary, Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| Page 17 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 6| 7| 4| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 5| 5| DAY ON TEST | 3| 3| 2| 3| 5| 3| 3| 3| 3| 3| 9| 2| 9| 1| 3| 8| 3| 5| 3| 3| 3| 8| 2| 7| 0| | 3| 3| 1| 3| 2| 3| 3| 3| 3| 3| 9| 6| 4| 3| 3| 4| 3| 9| 3| 3| 3| 4| 4| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 1000 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Deep Cervical, Leukemia Mononuclear | X | Iliac, Leukemia Mononuclear | X X | Mediastinal, Leukemia Mononuclear | X X X X | Pancreatic, Leukemia Mononuclear | X X | Renal, Leukemia Mononuclear | X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | Sarcoma, Metastatic, Skin | | Schwannoma Malignant, Metastatic, | | Uterus | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Fibroadenoma | X X X X X X X | Fibroadenoma, Multiple | | Leukemia Mononuclear | X X | Sarcoma, Metastatic, Skin | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | | Leukemia Mononuclear | X X | Subcutaneous Tissue, Fibrosarcoma | | Subcutaneous Tissue, Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Leukemia Mononuclear | X X X X X X X | Sarcoma, Metastatic, Skin | | Mediastinum, Lymphoma Malignant | | _____________________________________________________________________________________________________________________| Page 18 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 6| 7| 4| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 5| 5| DAY ON TEST | 3| 3| 2| 3| 5| 3| 3| 3| 3| 3| 9| 2| 9| 1| 3| 8| 3| 5| 3| 3| 3| 8| 2| 7| 0| | 3| 3| 1| 3| 2| 3| 3| 3| 3| 3| 9| 6| 4| 3| 3| 4| 3| 9| 3| 3| 3| 4| 4| 2| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 1000 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | Lymphocytic | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + + | __________________________________________________________________________| Harderian Gland | | __________________________________________________________________________| Zymbal's Gland | + + + M + M + + + M + + + + + + M + + M M M + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Lipoma | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + M + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | Lymphoma Malignant Lymphocytic | X | _____________________________________________________________________________________________________________________| Page 19 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 1000 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | | 50 | Leukemia Mononuclear | | 1 | Lipoma | | 1 | __________________________________________________________________________|____________| Intestine Large, Colon | | 47 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Large, Rectum | | 47 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Intestine Large, Cecum | | 47 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Small, Duodenum | | 48 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Small, Jejunum | | 45 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Small, Ileum | | 46 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Liver | | 50 | Leukemia Mononuclear | | 16 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Mesentery | | 6 | Sarcoma, Metastatic, Skin | | 1 | Schwannoma Malignant, Metastatic, | | | Uterus | | 1 | Fat, Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Pancreas | | 48 | Acinus, Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Pharynx | | 1 | Palate, Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Salivary Glands | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Stomach, Forestomach | | 49 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Stomach, Glandular | | 48 | Leukemia Mononuclear | | 4 | Sarcoma, Metastatic, Skin | | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | | 50 | Leukemia Mononuclear | | 12 | Schwannoma NOS | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 20 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 1000 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | | 50 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Adrenal Medulla | | 50 | Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Islets, Pancreatic | | 48 | Adenoma | | 1 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Parathyroid Gland | | 43 | __________________________________________________________________________|____________| Pituitary Gland | | 47 | Leukemia Mononuclear | | 10 | Pars Distalis, Adenoma | | 29 | Pars Distalis, Adenoma, Multiple | | 2 | Pars Intermedia, Adenoma | | 1 | __________________________________________________________________________|____________| Thyroid Gland | | 50 | Leukemia Mononuclear | | 1 | C-Cell, Adenoma | | 4 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | __________________________________________________________________________|____________| Tissue NOS | | 1 | Schwannoma Malignant, Metastatic, | | | Uterus | | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | | 48 | Carcinoma | | 1 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Ovary | | 50 | Granulosa Cell Tumor Benign | | 1 | Leukemia Mononuclear | | 9 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Uterus | | 50 | Adenoma | | 1 | Leukemia Mononuclear | | 5 | Polyp Stromal | | 12 | Sarcoma | | 1 | Schwannoma Malignant | | 1 | Cervix, Schwannoma Malignant, | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 21 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 1000 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | Metastatic, Uterus | | 1 | __________________________________________________________________________|____________| Vagina | | 1 | Schwannoma Malignant, Metastatic, | | | Uterus | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | | 49 | Leukemia Mononuclear | | 11 | __________________________________________________________________________|____________| Lymph Node | | 12 | Axillary, Leukemia Mononuclear | | 1 | Deep Cervical, Leukemia Mononuclear | | 1 | Iliac, Leukemia Mononuclear | | 2 | Mediastinal, Leukemia Mononuclear | | 6 | Pancreatic, Leukemia Mononuclear | | 3 | Renal, Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Lymph Node, Mandibular | | 50 | Leukemia Mononuclear | | 8 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | | 50 | Leukemia Mononuclear | | 12 | Sarcoma, Metastatic, Skin | | 1 | Schwannoma Malignant, Metastatic, | | | Uterus | | 1 | __________________________________________________________________________|____________| Spleen | | 49 | Leukemia Mononuclear | | 16 | __________________________________________________________________________|____________| Thymus | | 49 | Leukemia Mononuclear | | 8 | Lymphoma Malignant Lymphocytic | | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | | 50 | Adenocarcinoma | | 1 | Fibroadenoma | | 11 | Fibroadenoma, Multiple | | 1 | Leukemia Mononuclear | | 2 | Sarcoma, Metastatic, Skin | | 1 | __________________________________________________________________________|____________| Skin | | 50 | Keratoacanthoma | | 1 | Leukemia Mononuclear | | 2 | Subcutaneous Tissue, Fibrosarcoma | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 22 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | A | 1000 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | Subcutaneous Tissue, Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | | 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | | 50 | Leukemia Mononuclear | | 4 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | | 50 | Alveolar/Bronchiolar Adenoma | | 1 | Leukemia Mononuclear | | 12 | Sarcoma, Metastatic, Skin | | 1 | Mediastinum, Lymphoma Malignant | | | Lymphocytic | | 1 | __________________________________________________________________________|____________| Nose | | 50 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Trachea | | 50 | Leukemia Mononuclear | | 3 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 4 | __________________________________________________________________________|____________| Harderian Gland | | 1 | __________________________________________________________________________|____________| Zymbal's Gland | | 41 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | | 50 | Leukemia Mononuclear | | 10 | Lipoma | | 1 | __________________________________________________________________________|____________| Urinary Bladder | | 48 | Leukemia Mononuclear | | 3 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | | 50 | Leukemia Mononuclear | | 16 | Lymphoma Malignant Lymphocytic | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 23 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 5| 7| 4| 5| 7| 6| 7| 7| 7| 5| 7| 7| 6| 7| 7| 7| 7| 5| 7| 6| 7| DAY ON TEST | 3| 3| 6| 3| 4| 3| 2| 0| 0| 8| 3| 3| 3| 8| 3| 3| 7| 3| 3| 3| 3| 3| 3| 1| 3| | 0| 0| 0| 0| 0| 0| 8| 6| 6| 8| 0| 0| 0| 3| 0| 0| 4| 0| 0| 0| 0| 2| 0| 7| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + I| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + A + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + A + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + A + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + A + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Mesentery | + + | Fat, Sarcoma | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Leukemia Mononuclear | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Tongue | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Bilateral, Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Parathyroid Gland | M + + + + + + + + + + M + M M + + + + + + + + + M| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| Page 24 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 5| 7| 4| 5| 7| 6| 7| 7| 7| 5| 7| 7| 6| 7| 7| 7| 7| 5| 7| 6| 7| DAY ON TEST | 3| 3| 6| 3| 4| 3| 2| 0| 0| 8| 3| 3| 3| 8| 3| 3| 7| 3| 3| 3| 3| 3| 3| 1| 3| | 0| 0| 0| 0| 0| 0| 8| 6| 6| 8| 0| 0| 0| 3| 0| 0| 4| 0| 0| 0| 0| 2| 0| 7| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Pars Distalis, Adenoma | X X X X X X X X X X X X X X| Pars Distalis, Adenoma, Multiple | X | Pars Distalis, Carcinoma | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + M| Leukemia Mononuclear | X | C-Cell, Adenoma | X | Follicular Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leiomyosarcoma | | Leukemia Mononuclear | X | Polyp Stromal | X X X X X X | __________________________________________________________________________| Vagina | + | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Lymph Node | + + + | Mediastinal, Leukemia Mononuclear | | Renal, Leukemia Mononuclear | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + M + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | X X | Fibroadenoma | X X X X X X X | Fibroadenoma, Multiple | X | Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| Page 25 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 5| 7| 4| 5| 7| 6| 7| 7| 7| 5| 7| 7| 6| 7| 7| 7| 7| 5| 7| 6| 7| DAY ON TEST | 3| 3| 6| 3| 4| 3| 2| 0| 0| 8| 3| 3| 3| 8| 3| 3| 7| 3| 3| 3| 3| 3| 3| 1| 3| | 0| 0| 0| 0| 0| 0| 8| 6| 6| 8| 0| 0| 0| 3| 0| 0| 4| 0| 0| 0| 0| 2| 0| 7| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 3000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Subcutaneous Tissue, Fibrosarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Astrocytoma NOS | | Ependymoma Benign | X | Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosarcoma, Metastatic, Ear | X | Leukemia Mononuclear | X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + M| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | + | Pinna, Fibrosarcoma | X | __________________________________________________________________________| Eye | + + + | __________________________________________________________________________| Zymbal's Gland | + + M + + M M + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | _____________________________________________________________________________________________________________________| Page 26 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 5| 7| 7| 6| 7| 6| 7| 3| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| DAY ON TEST | 3| 3| 1| 5| 8| 3| 2| 2| 3| 6| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 0| 0| 0| 2| 7| 0| 6| 9| 0| 9| 0| 7| 0| 0| 0| 0| 0| 0| 0| 9| 0| 3| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 3000 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + A + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Small, Jejunum | + + + + A + + + + + + + + + + + + + + + + + + + +| Carcinoma | | Leukemia Mononuclear | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + A + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Mesentery | + | Fat, Sarcoma | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Leukemia Mononuclear | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Tongue | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Adrenal Medulla | M M + + + M + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Bilateral, Pheochromocytoma Benign | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | | __________________________________________________________________________| Parathyroid Gland | M M + + + + + + M + + + + M M + + + + + + + M + +| __________________________________________________________________________| Pituitary Gland | + M + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| Page 27 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 5| 7| 7| 6| 7| 6| 7| 3| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| DAY ON TEST | 3| 3| 1| 5| 8| 3| 2| 2| 3| 6| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 0| 0| 0| 2| 7| 0| 6| 9| 0| 9| 0| 7| 0| 0| 0| 0| 0| 0| 0| 9| 0| 3| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 3000 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Pars Distalis, Adenoma | X X X X X X X X X X X X X X X X| Pars Distalis, Adenoma, Multiple | X | Pars Distalis, Carcinoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | C-Cell, Adenoma | X | Follicular Cell, Adenoma | X X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + M + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leiomyosarcoma | X | Leukemia Mononuclear | X | Polyp Stromal | X X X X | __________________________________________________________________________| Vagina | + | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Lymph Node | + + + | Mediastinal, Leukemia Mononuclear | X X | Renal, Leukemia Mononuclear | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Thymus | M M + + + + + + + M + + + + + + M M M + M + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Fibroadenoma | X X X X X X X X X | Fibroadenoma, Multiple | X X| Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| Page 28 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 5| 7| 7| 6| 7| 6| 7| 3| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7| DAY ON TEST | 3| 3| 1| 5| 8| 3| 2| 2| 3| 6| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 0| 0| 0| 2| 7| 0| 6| 9| 0| 9| 0| 7| 0| 0| 0| 0| 0| 0| 0| 9| 0| 3| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 3000 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM - cont | | | | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | Subcutaneous Tissue, Fibrosarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Astrocytoma NOS | X | Ependymoma Benign | | Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibrosarcoma, Metastatic, Ear | | Leukemia Mononuclear | X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Ear | | Pinna, Fibrosarcoma | | __________________________________________________________________________| Eye | + + + | __________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| Page 29 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 3000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | | 49 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Intestine Large, Colon | | 49 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Intestine Large, Rectum | | 49 | __________________________________________________________________________|____________| Intestine Large, Cecum | | 48 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Intestine Small, Duodenum | | 50 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | | 49 | Carcinoma | | 1 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | | 48 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Liver | | 50 | Leukemia Mononuclear | | 9 | __________________________________________________________________________|____________| Mesentery | | 3 | Fat, Sarcoma | | 1 | __________________________________________________________________________|____________| Pancreas | | 50 | Acinus, Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Salivary Glands | | 50 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Stomach, Forestomach | | 50 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | | 50 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Tongue | | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | | 50 | Leukemia Mononuclear | | 4 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Adrenal Medulla | | 47 | Leukemia Mononuclear | | 3 | Bilateral, Pheochromocytoma Benign | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 30 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 3000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Adenoma | | 2 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Parathyroid Gland | | 39 | __________________________________________________________________________|____________| Pituitary Gland | | 49 | Leukemia Mononuclear | | 3 | Pars Distalis, Adenoma | | 30 | Pars Distalis, Adenoma, Multiple | | 2 | Pars Distalis, Carcinoma | | 1 | __________________________________________________________________________|____________| Thyroid Gland | | 49 | Leukemia Mononuclear | | 1 | C-Cell, Adenoma | | 2 | Follicular Cell, Adenoma | | 2 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | | 49 | Adenoma | | 2 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Ovary | | 50 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Uterus | | 50 | Leiomyosarcoma | | 1 | Leukemia Mononuclear | | 2 | Polyp Stromal | | 10 | __________________________________________________________________________|____________| Vagina | | 2 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Lymph Node | | 6 | Mediastinal, Leukemia Mononuclear | | 2 | Renal, Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | | 50 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Spleen | | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 31 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 3000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Leukemia Mononuclear | | 9 | __________________________________________________________________________|____________| Thymus | | 42 | Leukemia Mononuclear | | 2 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | | 50 | Adenocarcinoma | | 2 | Fibroadenoma | | 16 | Fibroadenoma, Multiple | | 3 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Skin | | 50 | Leukemia Mononuclear | | 1 | Subcutaneous Tissue, Fibrosarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | | 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | | 50 | Astrocytoma NOS | | 1 | Ependymoma Benign | | 1 | Leukemia Mononuclear | | 3 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | | 50 | Fibrosarcoma, Metastatic, Ear | | 1 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Nose | | 50 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Trachea | | 49 | Leukemia Mononuclear | | 1 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Ear | | 1 | Pinna, Fibrosarcoma | | 1 | __________________________________________________________________________|____________| Eye | | 6 | __________________________________________________________________________|____________| Zymbal's Gland | | 47 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 32 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| | A | 3000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Kidney | | 50 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Urinary Bladder | | 50 | Leukemia Mononuclear | | 1 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | | 50 | Leukemia Mononuclear | | 9 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 33 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 5| 6| 6| 5| 7| 7| 5| 6| 5| 7| 7| 6| 6| 6| 7| 2| 6| 7| 5| DAY ON TEST | 2| 3| 2| 2| 2| 2| 7| 5| 7| 6| 2| 2| 9| 5| 5| 2| 2| 6| 3| 5| 1| 2| 4| 2| 5| | 9| 7| 9| 9| 9| 9| 2| 8| 4| 0| 9| 9| 5| 8| 4| 9| 9| 5| 9| 8| 9| 6| 9| 9| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 10000PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + A + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + A + + + + + + + + A + + A + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + A + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + A + + + + + + + + A + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | __________________________________________________________________________| Mesentery | + + + + +| Fat, Leukemia Mononuclear | X | __________________________________________________________________________| Pancreas | + + + + + + + + + A + + + + + + + + + + + + + + +| Acinus, Leukemia Mononuclear | X X X | __________________________________________________________________________| Pharynx | + + | Palate, Squamous Cell Papilloma | X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Thyroid Gland | | Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | Pheochromocytoma Benign | | Bilateral, Pheochromocytoma Benign | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + A + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| Page 34 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 5| 6| 6| 5| 7| 7| 5| 6| 5| 7| 7| 6| 6| 6| 7| 2| 6| 7| 5| DAY ON TEST | 2| 3| 2| 2| 2| 2| 7| 5| 7| 6| 2| 2| 9| 5| 5| 2| 2| 6| 3| 5| 1| 2| 4| 2| 5| | 9| 7| 9| 9| 9| 9| 2| 8| 4| 0| 9| 9| 5| 8| 4| 9| 9| 5| 9| 8| 9| 6| 9| 9| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 10000PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + M + + + + + + M + + + +| __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | Pars Distalis, Adenoma | X X X X X X X X X X| Pars Distalis, Adenoma, Multiple | X | Pars Distalis, Carcinoma | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Bilateral, C-Cell, Adenoma | | C-Cell, Adenoma | X | C-Cell, Carcinoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + M + + + + + M + + + + + + + + + + +| Adenoma | X X | Leukemia Mononuclear | X X | Squamous Cell Carcinoma | X | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | Polyp Stromal | X X | __________________________________________________________________________| Vagina | | Fibroma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Lymph Node | + + + + + | Axillary, Leukemia Mononuclear | | Iliac, Leukemia Mononuclear | | Inguinal, Leukemia Mononuclear | X | Lumbar, Leukemia Mononuclear | X | Mediastinal, Leukemia Mononuclear | X X | Renal, Leukemia Mononuclear | X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Thyroid Gland | | Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + A + + + + + +| _____________________________________________________________________________________________________________________| Page 35 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 5| 6| 6| 5| 7| 7| 5| 6| 5| 7| 7| 6| 6| 6| 7| 2| 6| 7| 5| DAY ON TEST | 2| 3| 2| 2| 2| 2| 7| 5| 7| 6| 2| 2| 9| 5| 5| 2| 2| 6| 3| 5| 1| 2| 4| 2| 5| | 9| 7| 9| 9| 9| 9| 2| 8| 4| 0| 9| 9| 5| 8| 4| 9| 9| 5| 9| 8| 9| 6| 9| 9| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 10000PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Leukemia Mononuclear | X X X X X X X X X X X | __________________________________________________________________________| Thymus | + + + + + + M + + + + + + + + + + M + + + + + + +| Leukemia Mononuclear | X X X X X | Thymoma Benign | X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoacanthoma | | Adenocarcinoma | | Adenoma | | Fibroadenoma | X X X X X X | Leukemia Mononuclear | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Subcutaneous Tissue, Fibroma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Back, Sarcoma | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Pituitary | | Gland | X | Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Carcinoma, Metastatic, Thyroid Gland | | Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Thyroid Gland | | Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Zymbal's Gland | M + + + + + + + + + + + + + + + + + + + + + + + M| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | _____________________________________________________________________________________________________________________| Page 36 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 5| 6| 6| 5| 7| 7| 5| 6| 5| 7| 7| 6| 6| 6| 7| 2| 6| 7| 5| DAY ON TEST | 2| 3| 2| 2| 2| 2| 7| 5| 7| 6| 2| 2| 9| 5| 5| 2| 2| 6| 3| 5| 1| 2| 4| 2| 5| | 9| 7| 9| 9| 9| 9| 2| 8| 4| 0| 9| 9| 5| 8| 4| 9| 9| 5| 9| 8| 9| 6| 9| 9| 5| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 10000PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Urethra | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + A + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | _____________________________________________________________________________________________________________________| Page 37 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 2| 7| 7| 7| 6| 7| 6| 4| 7| 7| 7| 7| 5| 6| 5| 7| 1| 7| 0| 7| 7| 7| DAY ON TEST | 2| 2| 2| 9| 2| 2| 2| 1| 2| 6| 8| 2| 2| 2| 2| 0| 6| 0| 2| 7| 0| 8| 2| 2| 2| | 9| 9| 9| 6| 9| 9| 9| 8| 9| 0| 4| 9| 9| 9| 9| 2| 3| 2| 9| 6| 2| 5| 9| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 10000PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + A + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + A + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Mesentery | + | Fat, Leukemia Mononuclear | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + A + + +| Acinus, Leukemia Mononuclear | X X X | __________________________________________________________________________| Pharynx | | Palate, Squamous Cell Papilloma | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | X | Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + A + + +| Carcinoma, Metastatic, Thyroid Gland | X | Leukemia Mononuclear | X X X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | X X X | Pheochromocytoma Benign | X X | Bilateral, Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| Page 38 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 2| 7| 7| 7| 6| 7| 6| 4| 7| 7| 7| 7| 5| 6| 5| 7| 1| 7| 0| 7| 7| 7| DAY ON TEST | 2| 2| 2| 9| 2| 2| 2| 1| 2| 6| 8| 2| 2| 2| 2| 0| 6| 0| 2| 7| 0| 8| 2| 2| 2| | 9| 9| 9| 6| 9| 9| 9| 8| 9| 0| 4| 9| 9| 9| 9| 2| 3| 2| 9| 6| 2| 5| 9| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 10000PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Parathyroid Gland | M + + + + + M + + + + + + + + + + M + + + + + + M| __________________________________________________________________________| Pituitary Gland | M + + + + + + + + + + + + + + + + + M + + A + + +| Leukemia Mononuclear | X X X | Pars Distalis, Adenoma | X X X X X X X X X X| Pars Distalis, Adenoma, Multiple | | Pars Distalis, Carcinoma | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + M + + +| Leukemia Mononuclear | X X | Bilateral, C-Cell, Adenoma | X | C-Cell, Adenoma | X | C-Cell, Carcinoma | X X X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + M + + + + + + + +| Adenoma | | Leukemia Mononuclear | X | Squamous Cell Carcinoma | | __________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Polyp Stromal | X X X X X X X X | __________________________________________________________________________| Vagina | + + +| Fibroma | X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + A + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + | Axillary, Leukemia Mononuclear | X | Iliac, Leukemia Mononuclear | X | Inguinal, Leukemia Mononuclear | X | Lumbar, Leukemia Mononuclear | | Mediastinal, Leukemia Mononuclear | X X X X | Renal, Leukemia Mononuclear | X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Thyroid Gland | X | Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + A + + +| _____________________________________________________________________________________________________________________| Page 39 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 2| 7| 7| 7| 6| 7| 6| 4| 7| 7| 7| 7| 5| 6| 5| 7| 1| 7| 0| 7| 7| 7| DAY ON TEST | 2| 2| 2| 9| 2| 2| 2| 1| 2| 6| 8| 2| 2| 2| 2| 0| 6| 0| 2| 7| 0| 8| 2| 2| 2| | 9| 9| 9| 6| 9| 9| 9| 8| 9| 0| 4| 9| 9| 9| 9| 2| 3| 2| 9| 6| 2| 5| 9| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 10000PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Thymus | + + + + M + + + + + + + + + + + + + + M + A + + +| Leukemia Mononuclear | X X X | Thymoma Benign | | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoacanthoma | X | Adenocarcinoma | X | Adenoma | X | Fibroadenoma | X X X X | Leukemia Mononuclear | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Subcutaneous Tissue, Fibroma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | Back, Sarcoma | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Pituitary | | Gland | | Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Carcinoma, Metastatic, Thyroid Gland | X X | Leukemia Mononuclear | X X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma, Metastatic, Thyroid Gland | X X | Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Zymbal's Gland | + + + + + + + M + + + + + + + + + + + + + M + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | | _____________________________________________________________________________________________________________________| Page 40 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 2| 7| 7| 7| 6| 7| 6| 4| 7| 7| 7| 7| 5| 6| 5| 7| 1| 7| 0| 7| 7| 7| DAY ON TEST | 2| 2| 2| 9| 2| 2| 2| 1| 2| 6| 8| 2| 2| 2| 2| 0| 6| 0| 2| 7| 0| 8| 2| 2| 2| | 9| 9| 9| 6| 9| 9| 9| 8| 9| 0| 4| 9| 9| 9| 9| 2| 3| 2| 9| 6| 2| 5| 9| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 10000PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| URINARY SYSTEM - cont | | | | Leukemia Mononuclear | X X X X | __________________________________________________________________________| Urethra | + | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | _____________________________________________________________________________________________________________________| Page 41 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 10000PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | | 50 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Intestine Large, Colon | | 48 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Intestine Large, Rectum | | 48 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Intestine Large, Cecum | | 48 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Small, Duodenum | | 45 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Small, Jejunum | | 47 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Intestine Small, Ileum | | 46 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Liver | | 50 | Leukemia Mononuclear | | 15 | __________________________________________________________________________|____________| Mesentery | | 6 | Fat, Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Pancreas | | 48 | Acinus, Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Pharynx | | 2 | Palate, Squamous Cell Papilloma | | 2 | __________________________________________________________________________|____________| Salivary Glands | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Stomach, Forestomach | | 49 | Leukemia Mononuclear | | 5 | Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | | 48 | Leukemia Mononuclear | | 3 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | | 50 | Leukemia Mononuclear | | 8 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | | 49 | Carcinoma, Metastatic, Thyroid Gland | | 1 | Leukemia Mononuclear | | 8 | __________________________________________________________________________|____________| Adrenal Medulla | | 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 42 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 10000PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Leukemia Mononuclear | | 7 | Pheochromocytoma Benign | | 2 | Bilateral, Pheochromocytoma Benign | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | | 48 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Parathyroid Gland | | 44 | __________________________________________________________________________|____________| Pituitary Gland | | 47 | Leukemia Mononuclear | | 7 | Pars Distalis, Adenoma | | 20 | Pars Distalis, Adenoma, Multiple | | 1 | Pars Distalis, Carcinoma | | 2 | __________________________________________________________________________|____________| Thyroid Gland | | 49 | Leukemia Mononuclear | | 3 | Bilateral, C-Cell, Adenoma | | 1 | C-Cell, Adenoma | | 2 | C-Cell, Carcinoma | | 3 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Clitoral Gland | | 47 | Adenoma | | 2 | Leukemia Mononuclear | | 3 | Squamous Cell Carcinoma | | 1 | __________________________________________________________________________|____________| Ovary | | 50 | Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Uterus | | 50 | Leukemia Mononuclear | | 4 | Polyp Stromal | | 10 | __________________________________________________________________________|____________| Vagina | | 3 | Fibroma | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | | 49 | Leukemia Mononuclear | | 10 | __________________________________________________________________________|____________| Lymph Node | | 14 | Axillary, Leukemia Mononuclear | | 1 | Iliac, Leukemia Mononuclear | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 43 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 10000PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | Inguinal, Leukemia Mononuclear | | 2 | Lumbar, Leukemia Mononuclear | | 1 | Mediastinal, Leukemia Mononuclear | | 6 | Renal, Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Lymph Node, Mandibular | | 50 | Carcinoma, Metastatic, Thyroid Gland | | 1 | Leukemia Mononuclear | | 10 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | | 50 | Leukemia Mononuclear | | 10 | __________________________________________________________________________|____________| Spleen | | 48 | Leukemia Mononuclear | | 16 | __________________________________________________________________________|____________| Thymus | | 45 | Leukemia Mononuclear | | 8 | Thymoma Benign | | 1 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | | 50 | Adenoacanthoma | | 1 | Adenocarcinoma | | 1 | Adenoma | | 1 | Fibroadenoma | | 10 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Skin | | 50 | Leukemia Mononuclear | | 2 | Subcutaneous Tissue, Fibroma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | | 50 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | Back, Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | | 50 | Carcinoma, Metastatic, Pituitary | | | Gland | | 1 | Leukemia Mononuclear | | 3 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 44 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| | A | 10000PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - cont | | | | | | Alveolar/Bronchiolar Adenoma | | 2 | Carcinoma, Metastatic, Thyroid Gland | | 2 | Leukemia Mononuclear | | 11 | __________________________________________________________________________|____________| Nose | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Trachea | | 50 | Carcinoma, Metastatic, Thyroid Gland | | 2 | Leukemia Mononuclear | | 2 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Zymbal's Gland | | 46 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | | 50 | Carcinoma | | 1 | Leukemia Mononuclear | | 9 | __________________________________________________________________________|____________| Urethra | | 1 | __________________________________________________________________________|____________| Urinary Bladder | | 49 | Leukemia Mononuclear | | 4 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | | 50 | Leukemia Mononuclear | | 16 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 45 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 7| 4| 7| 7| 7| 7| 6| 7| 7| 6| 7| 5| 5| 6| 7| 7| 5| 7| 5| 6| 7| DAY ON TEST | 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 8| 3| 2| 3| 3| 4| 3| 0| 7| 3| | 3| 3| 3| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 0| 3| 1| 9| 9| 3| 3| 8| 4| 6| 3| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leiomyoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Rectum | A + + + + A + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Large, Cecum | A + + + + A + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + A + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Jejunum | A + + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | A + + + + A + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | X X X | Hepatocellular Adenoma, Multiple | | Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Mesentery | + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Acinus, Leukemia Mononuclear | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | Schwannoma Malignant | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Pheochromocytoma Malignant | | Pheochromocytoma Benign | X X X X X X X X | Bilateral, Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X | _____________________________________________________________________________________________________________________| Page 46 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 7| 4| 7| 7| 7| 7| 6| 7| 7| 6| 7| 5| 5| 6| 7| 7| 5| 7| 5| 6| 7| DAY ON TEST | 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 8| 3| 2| 3| 3| 4| 3| 0| 7| 3| | 3| 3| 3| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 0| 3| 1| 9| 9| 3| 3| 8| 4| 6| 3| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Leukemia Mononuclear | | __________________________________________________________________________| Parathyroid Gland | M + + + + + + M + + + + + + + + + + + M + + + M +| Leukemia Mononuclear | | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Pars Distalis, Adenoma | X X X X X X X X | Pars Distalis, Carcinoma | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | C-Cell, Adenoma | X X X | C-Cell, Carcinoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | Mesothelioma Malignant | X | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X | Bilateral, Adenoma | X | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Mesothelioma Malignant | X | Bilateral, Interstitial Cell, Adenoma| X X X X X X X X X X X X X X X X X| Interstitial Cell, Adenoma | X X X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + | Mediastinal, Leukemia Mononuclear | X X X | Pancreatic, Leukemia Mononuclear | X X | Pancreatic, Rhabdomyosarcoma, | | Metastatic, Skeletal Muscle | X | Renal, Leukemia Mononuclear | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + M + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| Page 47 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 7| 4| 7| 7| 7| 7| 6| 7| 7| 6| 7| 5| 5| 6| 7| 7| 5| 7| 5| 6| 7| DAY ON TEST | 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 8| 3| 2| 3| 3| 4| 3| 0| 7| 3| | 3| 3| 3| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 0| 3| 1| 9| 9| 3| 3| 8| 4| 6| 3| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | Rhabdomyosarcoma, Metastatic, | | Skeletal Muscle | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + M + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + M + + + + + + + + + + + +| Fibroadenoma | X X | Leukemia Mononuclear | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Basal Cell Adenoma | X | Keratoacanthoma | X | Leukemia Mononuclear | X | Squamous Cell Papilloma | | Subcutaneous Tissue, Fibroma | X X X | Subcutaneous Tissue, | | Neurofibrosarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + + | Abdominal, Mesothelioma Malignant | X | Abdominal, Rhabdomyosarcoma | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + + + + | __________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 48 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 2| 7| 7| 7| 7| 4| 7| 7| 7| 7| 6| 7| 7| 6| 7| 5| 5| 6| 7| 7| 5| 7| 5| 6| 7| DAY ON TEST | 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 8| 3| 2| 3| 3| 4| 3| 0| 7| 3| | 3| 3| 3| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 0| 3| 1| 9| 9| 3| 3| 8| 4| 6| 3| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | Carcinoma | X | Squamous Cell Papilloma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | Transitional Epithelium, Carcinoma | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | Mesothelioma Malignant | X | _____________________________________________________________________________________________________________________| Page 49 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 5| 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 6| 6| 7| 7| 7| DAY ON TEST | 3| 7| 3| 3| 1| 7| 1| 3| 7| 3| 3| 7| 3| 3| 3| 3| 0| 3| 3| 7| 1| 8| 3| 3| 3| | 4| 2| 4| 4| 5| 0| 4| 4| 9| 4| 4| 3| 4| 4| 4| 4| 7| 4| 4| 2| 8| 2| 4| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 0 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leiomyoma | | Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + A A + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + A A + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + A + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + A A + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | X | Hepatocellular Adenoma, Multiple | X | Leukemia Mononuclear | X X X X X X X X X X X | __________________________________________________________________________| Mesentery | + + + + | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | Acinus, Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | Schwannoma Malignant | X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + M + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Adrenal Medulla | + M + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Pheochromocytoma Malignant | X X | Pheochromocytoma Benign | X X X X X X | Bilateral, Pheochromocytoma Benign | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X | _____________________________________________________________________________________________________________________| Page 50 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 5| 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 6| 6| 7| 7| 7| DAY ON TEST | 3| 7| 3| 3| 1| 7| 1| 3| 7| 3| 3| 7| 3| 3| 3| 3| 0| 3| 3| 7| 1| 8| 3| 3| 3| | 4| 2| 4| 4| 5| 0| 4| 4| 9| 4| 4| 3| 4| 4| 4| 4| 7| 4| 4| 2| 8| 2| 4| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 0 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | Leukemia Mononuclear | X X X X | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + M + M + + + + + + + + + + M +| Leukemia Mononuclear | X X | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | Pars Distalis, Adenoma | X X X X X X X| Pars Distalis, Carcinoma | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | C-Cell, Adenoma | X X X X| C-Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | Mesothelioma Malignant | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Leukemia Mononuclear | X X X X | Bilateral, Adenoma | | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | Mesothelioma Malignant | | Bilateral, Interstitial Cell, Adenoma| X X X X X X X X X X X X X X X X X X X X X X X X| Interstitial Cell, Adenoma | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | __________________________________________________________________________| Lymph Node | + + + | Mediastinal, Leukemia Mononuclear | X X | Pancreatic, Leukemia Mononuclear | | Pancreatic, Rhabdomyosarcoma, | | Metastatic, Skeletal Muscle | | Renal, Leukemia Mononuclear | X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + M| Leukemia Mononuclear | X X X X X X X | _____________________________________________________________________________________________________________________| Page 51 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 5| 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 6| 6| 7| 7| 7| DAY ON TEST | 3| 7| 3| 3| 1| 7| 1| 3| 7| 3| 3| 7| 3| 3| 3| 3| 0| 3| 3| 7| 1| 8| 3| 3| 3| | 4| 2| 4| 4| 5| 0| 4| 4| 9| 4| 4| 3| 4| 4| 4| 4| 7| 4| 4| 2| 8| 2| 4| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 0 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | Rhabdomyosarcoma, Metastatic, | | Skeletal Muscle | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + M + + + + + + +| Leukemia Mononuclear | X X X X X X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + M + + + + + + + + + + + + + + + + + + +| Fibroadenoma | | Leukemia Mononuclear | X X X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Basal Cell Adenoma | X | Keratoacanthoma | | Leukemia Mononuclear | X X | Squamous Cell Papilloma | X | Subcutaneous Tissue, Fibroma | X | Subcutaneous Tissue, | | Neurofibrosarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Abdominal, Mesothelioma Malignant | | Abdominal, Rhabdomyosarcoma | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Leukemia Mononuclear | X X X X X X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Zymbal's Gland | + + + + + M + + + + + + + + + + + + + M + + + + +| _____________________________________________________________________________________________________________________| Page 52 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 5| 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 6| 6| 7| 7| 7| DAY ON TEST | 3| 7| 3| 3| 1| 7| 1| 3| 7| 3| 3| 7| 3| 3| 3| 3| 0| 3| 3| 7| 1| 8| 3| 3| 3| | 4| 2| 4| 4| 5| 0| 4| 4| 9| 4| 4| 3| 4| 4| 4| 4| 7| 4| 4| 2| 8| 2| 4| 4| 4| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 0 PPM | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | Carcinoma | | Squamous Cell Papilloma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X | Transitional Epithelium, Carcinoma | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | Mesothelioma Malignant | | _____________________________________________________________________________________________________________________| Page 53 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Large, Colon | | 50 | Leiomyoma | | 1 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Large, Rectum | | 46 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Intestine Large, Cecum | | 46 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Intestine Small, Duodenum | | 49 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Intestine Small, Jejunum | | 47 | __________________________________________________________________________|____________| Intestine Small, Ileum | | 46 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Liver | | 50 | Hepatocellular Adenoma | | 4 | Hepatocellular Adenoma, Multiple | | 1 | Leukemia Mononuclear | | 18 | __________________________________________________________________________|____________| Mesentery | | 5 | __________________________________________________________________________|____________| Pancreas | | 50 | Leukemia Mononuclear | | 2 | Acinus, Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Salivary Glands | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Stomach, Forestomach | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Stomach, Glandular | | 50 | Leukemia Mononuclear | | 5 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | | 50 | Leukemia Mononuclear | | 12 | Schwannoma Malignant | | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | | 49 | Leukemia Mononuclear | | 9 | __________________________________________________________________________|____________| Adrenal Medulla | | 49 | Leukemia Mononuclear | | 7 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 54 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Pheochromocytoma Malignant | | 2 | Pheochromocytoma Benign | | 14 | Bilateral, Pheochromocytoma Benign | | 1 | __________________________________________________________________________|____________| Islets, Pancreatic | | 50 | Adenoma | | 4 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Parathyroid Gland | | 43 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Pituitary Gland | | 50 | Leukemia Mononuclear | | 9 | Pars Distalis, Adenoma | | 15 | Pars Distalis, Carcinoma | | 2 | __________________________________________________________________________|____________| Thyroid Gland | | 50 | Leukemia Mononuclear | | 3 | C-Cell, Adenoma | | 7 | C-Cell, Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | | 50 | Leukemia Mononuclear | | 3 | Mesothelioma Malignant | | 1 | __________________________________________________________________________|____________| Preputial Gland | | 50 | Adenoma | | 1 | Leukemia Mononuclear | | 5 | Bilateral, Adenoma | | 1 | __________________________________________________________________________|____________| Prostate | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Seminal Vesicle | | 50 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Testes | | 50 | Leukemia Mononuclear | | 7 | Mesothelioma Malignant | | 1 | Bilateral, Interstitial Cell, Adenoma| | 41 | Interstitial Cell, Adenoma | | 4 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 55 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Bone Marrow | | 50 | Leukemia Mononuclear | | 14 | __________________________________________________________________________|____________| Lymph Node | | 15 | Mediastinal, Leukemia Mononuclear | | 5 | Pancreatic, Leukemia Mononuclear | | 2 | Pancreatic, Rhabdomyosarcoma, | | | Metastatic, Skeletal Muscle | | 1 | Renal, Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Lymph Node, Mandibular | | 48 | Leukemia Mononuclear | | 10 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | | 49 | Leukemia Mononuclear | | 12 | Rhabdomyosarcoma, Metastatic, | | | Skeletal Muscle | | 1 | __________________________________________________________________________|____________| Spleen | | 50 | Leukemia Mononuclear | | 18 | __________________________________________________________________________|____________| Thymus | | 48 | Leukemia Mononuclear | | 10 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | | 48 | Fibroadenoma | | 2 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Skin | | 50 | Basal Cell Adenoma | | 2 | Keratoacanthoma | | 1 | Leukemia Mononuclear | | 3 | Squamous Cell Papilloma | | 1 | Subcutaneous Tissue, Fibroma | | 4 | Subcutaneous Tissue, | | | Neurofibrosarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | | 50 | __________________________________________________________________________|____________| Skeletal Muscle | | 2 | Abdominal, Mesothelioma Malignant | | 1 | Abdominal, Rhabdomyosarcoma | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 56 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| | A | 0 PPM | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ NERVOUS SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Brain | | 50 | Leukemia Mononuclear | | 3 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | | 50 | Alveolar/Bronchiolar Adenoma | | 2 | Leukemia Mononuclear | | 15 | __________________________________________________________________________|____________| Nose | | 50 | Leukemia Mononuclear | | 8 | __________________________________________________________________________|____________| Trachea | | 50 | Leukemia Mononuclear | | 4 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 5 | __________________________________________________________________________|____________| Zymbal's Gland | | 48 | Carcinoma | | 1 | Squamous Cell Papilloma | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | | 50 | Leukemia Mononuclear | | 15 | Transitional Epithelium, Carcinoma | | 1 | __________________________________________________________________________|____________| Urinary Bladder | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | | 50 | Leukemia Mononuclear | | 18 | Mesothelioma Malignant | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 57 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 6| 6| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7| DAY ON TEST | 2| 0| 3| 3| 3| 4| 3| 3| 3| 2| 3| 3| 1| 3| 5| 8| 3| 3| 3| 7| 5| 3| 3| 3| 3| | 1| 1| 7| 0| 0| 1| 0| 0| 7| 1| 0| 0| 3| 0| 1| 5| 0| 0| 0| 3| 1| 3| 3| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | X X | Hepatocellular Adenoma, Multiple | | Leukemia Mononuclear | X X X X X X X X X X X X | __________________________________________________________________________| Mesentery | + + + + | Fat, Leukemia Mononuclear | X X | Fat, Mesothelioma Malignant | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Acinus, Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Pharynx | + | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | Pheochromocytoma Benign | X X X X| Bilateral, Pheochromocytoma Benign | X | _____________________________________________________________________________________________________________________| Page 58 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 6| 6| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7| DAY ON TEST | 2| 0| 3| 3| 3| 4| 3| 3| 3| 2| 3| 3| 1| 3| 5| 8| 3| 3| 3| 7| 5| 3| 3| 3| 3| | 1| 1| 7| 0| 0| 1| 0| 0| 7| 1| 0| 0| 3| 0| 1| 5| 0| 0| 0| 3| 1| 3| 3| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X | Leukemia Mononuclear | X X X X | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + M + + + + + + + + + + + +| Adenoma | | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | Pars Distalis, Adenoma | X X X X X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | C-Cell, Adenoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Coagulating Gland | + | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Mesothelioma Malignant | X | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X | Leukemia Mononuclear | X X X | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Mesothelioma Malignant | X | Bilateral, Interstitial Cell, Adenoma| X X X X X X X X X X X X X X X X X X X X X X | Interstitial Cell, Adenoma | X X X| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | | Leukemia Mononuclear | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | __________________________________________________________________________| Lymph Node | + + + + + + + | Axillary, Leukemia Mononuclear | X | Bronchial, Leukemia Mononuclear | X | Iliac, Leukemia Mononuclear | | Lumbar, Leukemia Mononuclear | | Mediastinal, Leukemia Mononuclear | X X X X X | _____________________________________________________________________________________________________________________| Page 59 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 6| 6| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7| DAY ON TEST | 2| 0| 3| 3| 3| 4| 3| 3| 3| 2| 3| 3| 1| 3| 5| 8| 3| 3| 3| 7| 5| 3| 3| 3| 3| | 1| 1| 7| 0| 0| 1| 0| 0| 7| 1| 0| 0| 3| 0| 1| 5| 0| 0| 0| 3| 1| 3| 3| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Pancreatic, Leukemia Mononuclear | X X | Renal, Leukemia Mononuclear | X X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X | __________________________________________________________________________| Thymus | + + + M + + + + + + + + + M + + + + M + + M + + +| Leukemia Mononuclear | X X X X X X X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + M + + + + + + + + + + + + +| Fibroadenoma | X | Fibroadenoma, Multiple | X | Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Basal Cell Adenoma | X X | Basal Cell Carcinoma | X | Basosquamous Tumor Benign | | Keratoacanthoma | X | Keratoacanthoma, Multiple | | Leukemia Mononuclear | X X X | Subcutaneous Tissue, Fibroma | X X X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | Lipoma | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Ependymoma Benign | X | Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 60 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 6| 6| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7| DAY ON TEST | 2| 0| 3| 3| 3| 4| 3| 3| 3| 2| 3| 3| 1| 3| 5| 8| 3| 3| 3| 7| 5| 3| 3| 3| 3| | 1| 1| 7| 0| 0| 1| 0| 0| 7| 1| 0| 0| 3| 0| 1| 5| 0| 0| 0| 3| 1| 3| 3| 3| 3| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | __________________________________________________________________________| Eye | + + | __________________________________________________________________________| Harderian Gland | + | __________________________________________________________________________| Zymbal's Gland | + + + + M + + + + + + + + + + + + + + M + + + + +| Carcinoma | X | Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X | Transitional Epithelium, Carcinoma | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X | Mesothelioma Malignant | X | _____________________________________________________________________________________________________________________| Page 61 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 6| 7| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 6| 7| 6| 7| 6| DAY ON TEST | 1| 1| 1| 2| 1| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 7| 7| 6| 1| 3| 4| 3| 7| | 5| 7| 4| 6| 4| 3| 9| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| 0| 0| 8| 3| 6| 3| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1000 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Rectum | + M + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X| __________________________________________________________________________| Intestine Large, Cecum | + + A + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X| __________________________________________________________________________| Intestine Small, Duodenum | + + A + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X| __________________________________________________________________________| Intestine Small, Jejunum | + + A + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Ileum | + + A + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X| __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | X X X | Hepatocellular Adenoma, Multiple | X | Leukemia Mononuclear | X X X X X X X X X X X X X| __________________________________________________________________________| Mesentery | + | Fat, Leukemia Mononuclear | | Fat, Mesothelioma Malignant | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | Acinus, Leukemia Mononuclear | X X X | __________________________________________________________________________| Pharynx | | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X| __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X| __________________________________________________________________________| Tooth | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X| __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X| Pheochromocytoma Benign | X X X | Bilateral, Pheochromocytoma Benign | X | _____________________________________________________________________________________________________________________| Page 62 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 6| 7| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 6| 7| 6| 7| 6| DAY ON TEST | 1| 1| 1| 2| 1| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 7| 7| 6| 1| 3| 4| 3| 7| | 5| 7| 4| 6| 4| 3| 9| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| 0| 0| 8| 3| 6| 3| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1000 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X | Leukemia Mononuclear | X X | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + M M + + +| Adenoma | X | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X| Pars Distalis, Adenoma | X X X X X X X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | C-Cell, Adenoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Coagulating Gland | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X| Mesothelioma Malignant | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X| Mesothelioma Malignant | | Bilateral, Interstitial Cell, Adenoma| X X X X X X X X X X X X X X X X X X X| Interstitial Cell, Adenoma | X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Blood | + | Leukemia Mononuclear | X | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X| __________________________________________________________________________| Lymph Node | + + + + + + + + +| Axillary, Leukemia Mononuclear | | Bronchial, Leukemia Mononuclear | | Iliac, Leukemia Mononuclear | X X | Lumbar, Leukemia Mononuclear | X | Mediastinal, Leukemia Mononuclear | X X X X X| _____________________________________________________________________________________________________________________| Page 63 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 6| 7| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 6| 7| 6| 7| 6| DAY ON TEST | 1| 1| 1| 2| 1| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 7| 7| 6| 1| 3| 4| 3| 7| | 5| 7| 4| 6| 4| 3| 9| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| 0| 0| 8| 3| 6| 3| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1000 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Pancreatic, Leukemia Mononuclear | X X | Renal, Leukemia Mononuclear | X X X| __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X| __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X| __________________________________________________________________________| Thymus | + + + + + + + M + M M M M M + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibroadenoma | X | Fibroadenoma, Multiple | | Leukemia Mononuclear | X X X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Basal Cell Adenoma | | Basal Cell Carcinoma | | Basosquamous Tumor Benign | X | Keratoacanthoma | X | Keratoacanthoma, Multiple | X | Leukemia Mononuclear | X X | Subcutaneous Tissue, Fibroma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Lipoma | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Ependymoma Benign | | Leukemia Mononuclear | X X X| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X| __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X| __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 64 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 7| 6| 7| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 6| 7| 6| 7| 6| DAY ON TEST | 1| 1| 1| 2| 1| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 7| 7| 6| 1| 3| 4| 3| 7| | 5| 7| 4| 6| 4| 3| 9| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| 0| 0| 8| 3| 6| 3| 1| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 1000 PPM | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM - cont | | | | __________________________________________________________________________| Eye | + + | __________________________________________________________________________| Harderian Gland | | __________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | | Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X| Transitional Epithelium, Carcinoma | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X| _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X X| Mesothelioma Malignant | | _____________________________________________________________________________________________________________________| Page 65 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 1000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | | 50 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Intestine Large, Colon | | 50 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Large, Rectum | | 49 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Intestine Large, Cecum | | 49 | Leukemia Mononuclear | | 11 | __________________________________________________________________________|____________| Intestine Small, Duodenum | | 49 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Intestine Small, Jejunum | | 49 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Intestine Small, Ileum | | 49 | Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Liver | | 50 | Hepatocellular Adenoma | | 5 | Hepatocellular Adenoma, Multiple | | 1 | Leukemia Mononuclear | | 25 | __________________________________________________________________________|____________| Mesentery | | 5 | Fat, Leukemia Mononuclear | | 2 | Fat, Mesothelioma Malignant | | 1 | __________________________________________________________________________|____________| Pancreas | | 50 | Leukemia Mononuclear | | 1 | Acinus, Leukemia Mononuclear | | 9 | __________________________________________________________________________|____________| Pharynx | | 1 | __________________________________________________________________________|____________| Salivary Glands | | 50 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Stomach, Forestomach | | 50 | Leukemia Mononuclear | | 8 | __________________________________________________________________________|____________| Stomach, Glandular | | 50 | Leukemia Mononuclear | | 9 | __________________________________________________________________________|____________| Tooth | | 1 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | | 50 | Leukemia Mononuclear | | 21 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 66 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 1000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - cont | | | | | | Leukemia Mononuclear | | 16 | __________________________________________________________________________|____________| Adrenal Medulla | | 50 | Leukemia Mononuclear | | 15 | Pheochromocytoma Benign | | 7 | Bilateral, Pheochromocytoma Benign | | 2 | __________________________________________________________________________|____________| Islets, Pancreatic | | 50 | Adenoma | | 5 | Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Parathyroid Gland | | 47 | Adenoma | | 1 | __________________________________________________________________________|____________| Pituitary Gland | | 50 | Leukemia Mononuclear | | 14 | Pars Distalis, Adenoma | | 14 | __________________________________________________________________________|____________| Thyroid Gland | | 50 | Leukemia Mononuclear | | 3 | C-Cell, Adenoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Coagulating Gland | | 1 | __________________________________________________________________________|____________| Epididymis | | 50 | Leukemia Mononuclear | | 2 | Mesothelioma Malignant | | 1 | __________________________________________________________________________|____________| Preputial Gland | | 50 | Adenoma | | 3 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Prostate | | 50 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Seminal Vesicle | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Testes | | 50 | Leukemia Mononuclear | | 9 | Mesothelioma Malignant | | 1 | Bilateral, Interstitial Cell, Adenoma| | 41 | Interstitial Cell, Adenoma | | 5 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 67 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 1000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Blood | | 1 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Bone Marrow | | 50 | Leukemia Mononuclear | | 23 | __________________________________________________________________________|____________| Lymph Node | | 16 | Axillary, Leukemia Mononuclear | | 1 | Bronchial, Leukemia Mononuclear | | 1 | Iliac, Leukemia Mononuclear | | 2 | Lumbar, Leukemia Mononuclear | | 1 | Mediastinal, Leukemia Mononuclear | | 10 | Pancreatic, Leukemia Mononuclear | | 4 | Renal, Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Lymph Node, Mandibular | | 50 | Leukemia Mononuclear | | 17 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | | 50 | Leukemia Mononuclear | | 18 | __________________________________________________________________________|____________| Spleen | | 50 | Leukemia Mononuclear | | 24 | __________________________________________________________________________|____________| Thymus | | 40 | Leukemia Mononuclear | | 15 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | | 49 | Fibroadenoma | | 2 | Fibroadenoma, Multiple | | 1 | Leukemia Mononuclear | | 9 | __________________________________________________________________________|____________| Skin | | 50 | Basal Cell Adenoma | | 2 | Basal Cell Carcinoma | | 1 | Basosquamous Tumor Benign | | 1 | Keratoacanthoma | | 2 | Keratoacanthoma, Multiple | | 1 | Leukemia Mononuclear | | 5 | Subcutaneous Tissue, Fibroma | | 4 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | | 50 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | Lipoma | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 68 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| | A | 1000 PPM | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | | 50 | Ependymoma Benign | | 1 | Leukemia Mononuclear | | 7 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | | 50 | Leukemia Mononuclear | | 21 | __________________________________________________________________________|____________| Nose | | 50 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Trachea | | 50 | Leukemia Mononuclear | | 2 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 4 | __________________________________________________________________________|____________| Harderian Gland | | 1 | __________________________________________________________________________|____________| Zymbal's Gland | | 48 | Carcinoma | | 1 | Leukemia Mononuclear | | 2 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | | 50 | Leukemia Mononuclear | | 18 | Transitional Epithelium, Carcinoma | | 1 | __________________________________________________________________________|____________| Urinary Bladder | | 50 | Leukemia Mononuclear | | 8 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | | 50 | Leukemia Mononuclear | | 25 | Mesothelioma Malignant | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 69 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 5| 6| 7| 6| 5| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 7| 5| 7| 7| 7| DAY ON TEST | 2| 8| 2| 8| 7| 2| 7| 3| 2| 2| 2| 2| 2| 2| 3| 9| 2| 2| 2| 8| 3| 3| 3| 3| 3| | 9| 6| 9| 1| 1| 9| 7| 7| 9| 9| 9| 9| 9| 9| 2| 2| 9| 9| 9| 0| 0| 3| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Sarcoma, Metastatic, Spleen | | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + A + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + A + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Sarcoma, Metastatic, Spleen | | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + A + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + A A + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | X | Hepatocellular Adenoma, Multiple | | Leukemia Mononuclear | X X X X X X X X X X X X| Sarcoma, Metastatic, Spleen | | __________________________________________________________________________| Mesentery | + + | Sarcoma, Metastatic, Spleen | | Fat, Leukemia Mononuclear | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Sarcoma, Metastatic, Spleen | | Acinus, Leukemia Mononuclear | X X X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | | Leukemia Mononuclear | X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Sarcoma, Metastatic, Spleen | | Squamous Cell Carcinoma | | Squamous Cell Papilloma | X X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Tooth | | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X| Schwannoma Malignant | X | _____________________________________________________________________________________________________________________| Page 70 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 5| 6| 7| 6| 5| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 7| 5| 7| 7| 7| DAY ON TEST | 2| 8| 2| 8| 7| 2| 7| 3| 2| 2| 2| 2| 2| 2| 3| 9| 2| 2| 2| 8| 3| 3| 3| 3| 3| | 9| 6| 9| 1| 1| 9| 7| 7| 9| 9| 9| 9| 9| 9| 2| 2| 9| 9| 9| 0| 0| 3| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Sarcoma, Metastatic, Spleen | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | Mixed Tumor Malignant | X | Pheochromocytoma Malignant | X | Pheochromocytoma Benign | X X X | Sarcoma, Metastatic, Spleen | | Bilateral, Pheochromocytoma Benign | | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Carcinoma | | Leukemia Mononuclear | X X X | __________________________________________________________________________| Parathyroid Gland | + + + + M + + + + + + M + + + + + + M + + + + + +| Adenoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + M + + + + + + + + +| Leukemia Mononuclear | X X X X | Pars Distalis, Adenoma | X X X X X X X X X | Pars Distalis, Adenoma, Multiple | X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | C-Cell, Adenoma | X | C-Cell, Carcinoma | | Follicular Cell, Carcinoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Mesothelioma Malignant | X | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X | Leukemia Mononuclear | X | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 71 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 5| 6| 7| 6| 5| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 7| 5| 7| 7| 7| DAY ON TEST | 2| 8| 2| 8| 7| 2| 7| 3| 2| 2| 2| 2| 2| 2| 3| 9| 2| 2| 2| 8| 3| 3| 3| 3| 3| | 9| 6| 9| 1| 1| 9| 7| 7| 9| 9| 9| 9| 9| 9| 2| 2| 9| 9| 9| 0| 0| 3| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | Leukemia Mononuclear | X X X X| Mesothelioma Malignant | X | Sarcoma, Metastatic, Spleen | | Bilateral, Interstitial Cell, Adenoma| X X X X X X X X X X X X X X X X X X X X X| Interstitial Cell, Adenoma | X X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X| __________________________________________________________________________| Lymph Node | + + + + + + + + + + + | Axillary, Leukemia Mononuclear | | Bronchial, Leukemia Mononuclear | X | Iliac, Leukemia Mononuclear | X | Inguinal, Leukemia Mononuclear | | Lumbar, Leukemia Mononuclear | X | Mediastinal, Leukemia Mononuclear | X X X X X X | Pancreatic, Leukemia Mononuclear | X X X | Renal, Leukemia Mononuclear | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X| Sarcoma, Metastatic, Spleen | | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X| Mesothelioma Malignant | X | Sarcoma | | __________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibroadenoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | X | Leukemia Mononuclear | X | Sebaceous Gland, Adenoma | | Subcutaneous Tissue, Fibroma | X X X X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 72 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 6| 7| 5| 6| 7| 6| 5| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 7| 5| 7| 7| 7| DAY ON TEST | 2| 8| 2| 8| 7| 2| 7| 3| 2| 2| 2| 2| 2| 2| 3| 9| 2| 2| 2| 8| 3| 3| 3| 3| 3| | 9| 6| 9| 1| 1| 9| 7| 7| 9| 9| 9| 9| 9| 9| 2| 2| 9| 9| 9| 0| 0| 3| 0| 0| 0| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM - cont | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Spinal Cord | + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Leukemia Mononuclear | X X X X X X X X X X X X| Pheochromocytoma Malignant, | | Metastatic, Adrenal Medulla | | Sarcoma, Metastatic, Spleen | | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + M + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + + + | __________________________________________________________________________| Zymbal's Gland | + M + + + + + + + + + + + + + M + + + + + + + + +| Carcinoma | X | Leukemia Mononuclear | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X| Renal Tubule, Adenoma | X| __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X| Mesothelioma Malignant | X | _____________________________________________________________________________________________________________________| Page 73 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 3| 7| 7| 7| 7| 6| 6| 6| DAY ON TEST | 7| 0| 2| 3| 3| 9| 3| 3| 3| 3| 3| 1| 0| 3| 3| 3| 7| 5| 2| 3| 3| 3| 9| 4| 7| | 0| 0| 1| 0| 0| 2| 0| 0| 0| 0| 0| 4| 9| 0| 0| 0| 4| 6| 5| 0| 0| 0| 4| 9| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3000 PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Sarcoma, Metastatic, Spleen | X | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Sarcoma, Metastatic, Spleen | X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | | Hepatocellular Adenoma, Multiple | X | Leukemia Mononuclear | X X X X X X X X X X X | Sarcoma, Metastatic, Spleen | X | __________________________________________________________________________| Mesentery | + + + + + | Sarcoma, Metastatic, Spleen | X | Fat, Leukemia Mononuclear | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | Sarcoma, Metastatic, Spleen | X | Acinus, Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | Leukemia Mononuclear | X X X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Sarcoma, Metastatic, Spleen | X | Squamous Cell Carcinoma | X | Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Tooth | + | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | Schwannoma Malignant | | _____________________________________________________________________________________________________________________| Page 74 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 3| 7| 7| 7| 7| 6| 6| 6| DAY ON TEST | 7| 0| 2| 3| 3| 9| 3| 3| 3| 3| 3| 1| 0| 3| 3| 3| 7| 5| 2| 3| 3| 3| 9| 4| 7| | 0| 0| 1| 0| 0| 2| 0| 0| 0| 0| 0| 4| 9| 0| 0| 0| 4| 6| 5| 0| 0| 0| 4| 9| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3000 PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM - cont | | | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Sarcoma, Metastatic, Spleen | X | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Mixed Tumor Malignant | | Pheochromocytoma Malignant | X | Pheochromocytoma Benign | X X X X X X | Sarcoma, Metastatic, Spleen | X | Bilateral, Pheochromocytoma Benign | X X X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X | Carcinoma | X | Leukemia Mononuclear | X | __________________________________________________________________________| Parathyroid Gland | + M + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Leukemia Mononuclear | | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + M + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | Pars Distalis, Adenoma | X X X X X X | Pars Distalis, Adenoma, Multiple | | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | C-Cell, Adenoma | X X X | C-Cell, Carcinoma | X | Follicular Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | Mesothelioma Malignant | | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Leukemia Mononuclear | X X | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 75 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 3| 7| 7| 7| 7| 6| 6| 6| DAY ON TEST | 7| 0| 2| 3| 3| 9| 3| 3| 3| 3| 3| 1| 0| 3| 3| 3| 7| 5| 2| 3| 3| 3| 9| 4| 7| | 0| 0| 1| 0| 0| 2| 0| 0| 0| 0| 0| 4| 9| 0| 0| 0| 4| 6| 5| 0| 0| 0| 4| 9| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3000 PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| GENITAL SYSTEM - cont | | | | Leukemia Mononuclear | X X X X | Mesothelioma Malignant | | Sarcoma, Metastatic, Spleen | X | Bilateral, Interstitial Cell, Adenoma| X X X X X X X X X X X X X X X X X X X X X X| Interstitial Cell, Adenoma | X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | __________________________________________________________________________| Lymph Node | + + + + + + + + + + + + | Axillary, Leukemia Mononuclear | X X | Bronchial, Leukemia Mononuclear | X | Iliac, Leukemia Mononuclear | X | Inguinal, Leukemia Mononuclear | X | Lumbar, Leukemia Mononuclear | X X | Mediastinal, Leukemia Mononuclear | X X X X X X X X | Pancreatic, Leukemia Mononuclear | X X X | Renal, Leukemia Mononuclear | X X X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + M + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X | Sarcoma, Metastatic, Spleen | X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | Mesothelioma Malignant | | Sarcoma | X | __________________________________________________________________________| Thymus | + + + + + + + + + + + + M + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Fibroadenoma | | Leukemia Mononuclear | X X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | X | Leukemia Mononuclear | X X | Sebaceous Gland, Adenoma | X| Subcutaneous Tissue, Fibroma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 76 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 3| 7| 7| 7| 7| 6| 6| 6| DAY ON TEST | 7| 0| 2| 3| 3| 9| 3| 3| 3| 3| 3| 1| 0| 3| 3| 3| 7| 5| 2| 3| 3| 3| 9| 4| 7| | 0| 0| 1| 0| 0| 2| 0| 0| 0| 0| 0| 4| 9| 0| 0| 0| 4| 6| 5| 0| 0| 0| 4| 9| 7| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3000 PPM | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM - cont | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Spinal Cord | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | | Leukemia Mononuclear | X X X X X X X X X X X | Pheochromocytoma Malignant, | | Metastatic, Adrenal Medulla | X | Sarcoma, Metastatic, Spleen | X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | | __________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | | Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X | Renal Tubule, Adenoma | X | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | Mesothelioma Malignant | | _____________________________________________________________________________________________________________________| Page 77 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | | 50 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Intestine Large, Colon | | 50 | Leukemia Mononuclear | | 4 | Sarcoma, Metastatic, Spleen | | 1 | __________________________________________________________________________|____________| Intestine Large, Rectum | | 49 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Large, Cecum | | 49 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Intestine Small, Duodenum | | 50 | Leukemia Mononuclear | | 6 | Sarcoma, Metastatic, Spleen | | 1 | __________________________________________________________________________|____________| Intestine Small, Jejunum | | 49 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Small, Ileum | | 48 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Liver | | 50 | Hepatocellular Adenoma | | 1 | Hepatocellular Adenoma, Multiple | | 1 | Leukemia Mononuclear | | 23 | Sarcoma, Metastatic, Spleen | | 1 | __________________________________________________________________________|____________| Mesentery | | 7 | Sarcoma, Metastatic, Spleen | | 1 | Fat, Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Pancreas | | 50 | Leukemia Mononuclear | | 1 | Sarcoma, Metastatic, Spleen | | 1 | Acinus, Leukemia Mononuclear | | 10 | __________________________________________________________________________|____________| Salivary Glands | | 50 | Carcinoma | | 1 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Stomach, Forestomach | | 50 | Leukemia Mononuclear | | 3 | Sarcoma, Metastatic, Spleen | | 1 | Squamous Cell Carcinoma | | 1 | Squamous Cell Papilloma | | 2 | __________________________________________________________________________|____________| Stomach, Glandular | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Tooth | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 78 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | | 50 | Leukemia Mononuclear | | 18 | Schwannoma Malignant | | 1 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | | 50 | Leukemia Mononuclear | | 10 | Sarcoma, Metastatic, Spleen | | 1 | __________________________________________________________________________|____________| Adrenal Medulla | | 50 | Leukemia Mononuclear | | 11 | Mixed Tumor Malignant | | 1 | Pheochromocytoma Malignant | | 2 | Pheochromocytoma Benign | | 9 | Sarcoma, Metastatic, Spleen | | 1 | Bilateral, Pheochromocytoma Benign | | 3 | __________________________________________________________________________|____________| Islets, Pancreatic | | 50 | Adenoma | | 3 | Carcinoma | | 1 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Parathyroid Gland | | 46 | Adenoma | | 1 | Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Pituitary Gland | | 48 | Leukemia Mononuclear | | 10 | Pars Distalis, Adenoma | | 15 | Pars Distalis, Adenoma, Multiple | | 1 | __________________________________________________________________________|____________| Thyroid Gland | | 50 | Leukemia Mononuclear | | 3 | C-Cell, Adenoma | | 4 | C-Cell, Carcinoma | | 1 | Follicular Cell, Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 79 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | Leukemia Mononuclear | | 2 | Mesothelioma Malignant | | 1 | __________________________________________________________________________|____________| Preputial Gland | | 50 | Adenoma | | 2 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Prostate | | 50 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Seminal Vesicle | | 50 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Testes | | 50 | Leukemia Mononuclear | | 8 | Mesothelioma Malignant | | 1 | Sarcoma, Metastatic, Spleen | | 1 | Bilateral, Interstitial Cell, Adenoma| | 43 | Interstitial Cell, Adenoma | | 5 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | | 50 | Leukemia Mononuclear | | 21 | __________________________________________________________________________|____________| Lymph Node | | 23 | Axillary, Leukemia Mononuclear | | 2 | Bronchial, Leukemia Mononuclear | | 2 | Iliac, Leukemia Mononuclear | | 2 | Inguinal, Leukemia Mononuclear | | 1 | Lumbar, Leukemia Mononuclear | | 3 | Mediastinal, Leukemia Mononuclear | | 14 | Pancreatic, Leukemia Mononuclear | | 6 | Renal, Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Lymph Node, Mandibular | | 49 | Leukemia Mononuclear | | 17 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | | 50 | Leukemia Mononuclear | | 19 | Sarcoma, Metastatic, Spleen | | 1 | __________________________________________________________________________|____________| Spleen | | 50 | Leukemia Mononuclear | | 23 | Mesothelioma Malignant | | 1 | Sarcoma | | 1 | __________________________________________________________________________|____________| Thymus | | 49 | Leukemia Mononuclear | | 15 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 80 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | | 50 | Fibroadenoma | | 1 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Skin | | 50 | Keratoacanthoma | | 2 | Leukemia Mononuclear | | 3 | Sebaceous Gland, Adenoma | | 1 | Subcutaneous Tissue, Fibroma | | 5 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | | 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | | 50 | Leukemia Mononuclear | | 6 | __________________________________________________________________________|____________| Spinal Cord | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | | 50 | Alveolar/Bronchiolar Adenoma | | 1 | Leukemia Mononuclear | | 23 | Pheochromocytoma Malignant, | | | Metastatic, Adrenal Medulla | | 1 | Sarcoma, Metastatic, Spleen | | 1 | __________________________________________________________________________|____________| Nose | | 49 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Trachea | | 50 | Leukemia Mononuclear | | 2 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 3 | __________________________________________________________________________|____________| Zymbal's Gland | | 48 | Carcinoma | | 1 | Leukemia Mononuclear | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | | 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 81 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | A | 3000 PPM | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ URINARY SYSTEM - cont | | | | | | Leukemia Mononuclear | | 19 | Renal Tubule, Adenoma | | 2 | __________________________________________________________________________|____________| Urinary Bladder | | 50 | Leukemia Mononuclear | | 6 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | | 50 | Leukemia Mononuclear | | 23 | Mesothelioma Malignant | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 82 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7| DAY ON TEST | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2| | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 10000PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenocarcinoma | | Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Intestine Small, Ileum | + + + + A + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | X X X | Histiocytic Sarcoma | | Leukemia Mononuclear | X X X X X X X X X X| __________________________________________________________________________| Mesentery | + + + | Fat, Leukemia Mononuclear | | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | | Acinus, Leukemia Mononuclear | X X X X | __________________________________________________________________________| Pharynx | + + + + + | Palate, Basosquamous Tumor Benign | | Palate, Squamous Cell Carcinoma | | Palate, Squamous Cell Papilloma | X X X X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Squamous Cell Papilloma | X | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X X X X X X | _____________________________________________________________________________________________________________________| Page 83 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7| DAY ON TEST | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2| | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 10000PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | Pheochromocytoma Malignant | X | Pheochromocytoma Benign | X X X X | Bilateral, Pheochromocytoma Benign | X X X X| __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X | Leukemia Mononuclear | X X X | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + M + + + M + + + + M + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X | Pars Distalis, Adenoma | X X X X | Pars Distalis, Adenoma, Multiple | X| __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | C-Cell, Adenoma | X X X | C-Cell, Carcinoma | | Follicular Cell, Carcinoma | X | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X | Leukemia Mononuclear | X | Squamous Cell Papilloma | X | __________________________________________________________________________| Prostate | + + + + + M + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Seminal Vesicle | + + + + + M + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | Bilateral, Interstitial Cell, Adenoma| X X X X X X X X X X X X X X X X X X X| Interstitial Cell, Adenoma | X X X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Leukemia Mononuclear | X X X X X X X X X| __________________________________________________________________________| Lymph Node | + + + + + + + + + +| _____________________________________________________________________________________________________________________| Page 84 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7| DAY ON TEST | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2| | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 10000PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Leukemia Mononuclear | X | Bronchial, Leukemia Mononuclear | X | Iliac, Leukemia Mononuclear | X | Inguinal, Leukemia Mononuclear | | Lumbar, Leukemia Mononuclear | X| Mediastinal, Leukemia Mononuclear | X X | Pancreatic, Leukemia Mononuclear | X X | Renal, Leukemia Mononuclear | X | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X| __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X| Sarcoma | | __________________________________________________________________________| Thymus | + + M + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + M M + + M + + + + +| Adenocarcinoma | X | Fibroadenoma | X | Histiocytic Sarcoma | | Leukemia Mononuclear | X X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | X X | Leukemia Mononuclear | X | Squamous Cell Papilloma | X | Sebaceous Gland, Adenoma | X | Subcutaneous Tissue, Fibroma | | Subcutaneous Tissue, Fibrosarcoma | X | Subcutaneous Tissue, Histiocytic | | Sarcoma | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | | Histiocytic Sarcoma | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 85 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7| DAY ON TEST | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2| | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 10000PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Histiocytic Sarcoma | | Leukemia Mononuclear | X X X X X X X X X X| __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Zymbal's Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Carcinoma | X | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X| __________________________________________________________________________| Urethra | + | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | | Leukemia Mononuclear | X X X X X X X X X X X| _____________________________________________________________________________________________________________________| Page 86 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7| DAY ON TEST | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2| | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 10000PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ALIMENTARY SYSTEM | | | | __________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + A + + + + + + + + + + + +| Adenocarcinoma | X | Leukemia Mononuclear | X X X X X | __________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + A + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + A + + + + + + + + A A + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + A + + + + + + + + + A + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Hepatocellular Adenoma | X X X | Histiocytic Sarcoma | X X | Leukemia Mononuclear | X X X X X X X X X X X X X X X | __________________________________________________________________________| Mesentery | + | Fat, Leukemia Mononuclear | X | __________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | Acinus, Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Pharynx | + + + + | Palate, Basosquamous Tumor Benign | X | Palate, Squamous Cell Carcinoma | X | Palate, Squamous Cell Papilloma | X | __________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | __________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | Squamous Cell Papilloma | | __________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | | | __________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | | | __________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | | Leukemia Mononuclear | X X X X X X X X | _____________________________________________________________________________________________________________________| Page 87 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7| DAY ON TEST | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2| | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 10000PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM - cont | | | | __________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X | Pheochromocytoma Malignant | | Pheochromocytoma Benign | X X X | Bilateral, Pheochromocytoma Benign | X | __________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Adenoma | X X X X | Leukemia Mononuclear | X X X X X X X | __________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + M + + + + + + + + + + + + M +| Leukemia Mononuclear | X | __________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | Pars Distalis, Adenoma | X X X X X | Pars Distalis, Adenoma, Multiple | X X | __________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X | C-Cell, Adenoma | X | C-Cell, Carcinoma | X | Follicular Cell, Carcinoma | | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | | | __________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + M + + + + + + +| Adenoma | X | Leukemia Mononuclear | X | Squamous Cell Papilloma | | __________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | __________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | __________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | Bilateral, Interstitial Cell, Adenoma| X X X X X X X X X X X X X X| Interstitial Cell, Adenoma | X X X X X X X | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | | | __________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Leukemia Mononuclear | X X X X X X X X X X X X X | __________________________________________________________________________| Lymph Node | + + + + + + + + | _____________________________________________________________________________________________________________________| Page 88 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7| DAY ON TEST | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2| | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 10000PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | | | Leukemia Mononuclear | | Bronchial, Leukemia Mononuclear | | Iliac, Leukemia Mononuclear | | Inguinal, Leukemia Mononuclear | X | Lumbar, Leukemia Mononuclear | X | Mediastinal, Leukemia Mononuclear | X X X | Pancreatic, Leukemia Mononuclear | X X X | Renal, Leukemia Mononuclear | | __________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X | __________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X | __________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + A + +| Leukemia Mononuclear | X X X X X X X X X X X X X X | Sarcoma | X | __________________________________________________________________________| Thymus | + + + + + + + + + + M + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | | | __________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + M + +| Adenocarcinoma | | Fibroadenoma | | Histiocytic Sarcoma | X | Leukemia Mononuclear | X X | __________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Keratoacanthoma | X | Leukemia Mononuclear | X X X | Squamous Cell Papilloma | | Sebaceous Gland, Adenoma | | Subcutaneous Tissue, Fibroma | X X | Subcutaneous Tissue, Fibrosarcoma | | Subcutaneous Tissue, Histiocytic | | Sarcoma | X | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | | | __________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________________________________________| Skeletal Muscle | + | Histiocytic Sarcoma | X | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | | | __________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | | | _____________________________________________________________________________________________________________________| Page 89 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 _____________________________________________________________________________________________________________________ | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7| DAY ON TEST | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2| | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9| _____________________________________________________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 10000PPM | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM - cont | | | | __________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Alveolar/Bronchiolar Adenoma | X | Histiocytic Sarcoma | X | Leukemia Mononuclear | X X X X X X X X X X X X X | __________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X | __________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X | _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | | | __________________________________________________________________________| Eye | + | __________________________________________________________________________| Zymbal's Gland | M + + + + + + + + + + + + + + + + + M + + + + + +| Carcinoma | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | | | __________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X X X X X X X X X X X X | __________________________________________________________________________| Urethra | | __________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| Leukemia Mononuclear | X X X X | _____________________________________________________________________________________________________________________| SYSTEMIC LESIONS | | __________________________________________________________________________| Multiple Organs | + + + + + + + + + + + + + + + + + + + + + + + + +| Histiocytic Sarcoma | X X | Leukemia Mononuclear | X X X X X X X X X X X X X X X | _____________________________________________________________________________________________________________________| Page 90 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 10000PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Esophagus | | 50 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Intestine Large, Colon | | 50 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Intestine Large, Rectum | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Intestine Large, Cecum | | 49 | Adenocarcinoma | | 1 | Leukemia Mononuclear | | 8 | __________________________________________________________________________|____________| Intestine Small, Duodenum | | 48 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Intestine Small, Jejunum | | 46 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Intestine Small, Ileum | | 47 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Liver | | 50 | Hepatocellular Adenoma | | 6 | Histiocytic Sarcoma | | 2 | Leukemia Mononuclear | | 25 | __________________________________________________________________________|____________| Mesentery | | 4 | Fat, Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Pancreas | | 50 | Leukemia Mononuclear | | 3 | Acinus, Leukemia Mononuclear | | 11 | __________________________________________________________________________|____________| Pharynx | | 9 | Palate, Basosquamous Tumor Benign | | 1 | Palate, Squamous Cell Carcinoma | | 1 | Palate, Squamous Cell Papilloma | | 5 | __________________________________________________________________________|____________| Salivary Glands | | 50 | Leukemia Mononuclear | | 5 | __________________________________________________________________________|____________| Stomach, Forestomach | | 50 | Leukemia Mononuclear | | 5 | Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Stomach, Glandular | | 50 | Leukemia Mononuclear | | 6 | _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | | | | __________________________________________________________________________|____________| Heart | | 50 | Leukemia Mononuclear | | 19 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 91 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 10000PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM - cont | | | | | | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | | | | __________________________________________________________________________|____________| Adrenal Cortex | | 50 | Adenoma | | 1 | Leukemia Mononuclear | | 14 | __________________________________________________________________________|____________| Adrenal Medulla | | 50 | Leukemia Mononuclear | | 14 | Pheochromocytoma Malignant | | 1 | Pheochromocytoma Benign | | 7 | Bilateral, Pheochromocytoma Benign | | 5 | __________________________________________________________________________|____________| Islets, Pancreatic | | 50 | Adenoma | | 7 | Leukemia Mononuclear | | 10 | __________________________________________________________________________|____________| Parathyroid Gland | | 45 | Leukemia Mononuclear | | 2 | __________________________________________________________________________|____________| Pituitary Gland | | 50 | Leukemia Mononuclear | | 18 | Pars Distalis, Adenoma | | 9 | Pars Distalis, Adenoma, Multiple | | 3 | __________________________________________________________________________|____________| Thyroid Gland | | 50 | Leukemia Mononuclear | | 5 | C-Cell, Adenoma | | 4 | C-Cell, Carcinoma | | 1 | Follicular Cell, Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | | | | __________________________________________________________________________|____________| Epididymis | | 50 | Leukemia Mononuclear | | 3 | __________________________________________________________________________|____________| Preputial Gland | | 49 | Adenoma | | 2 | Leukemia Mononuclear | | 2 | Squamous Cell Papilloma | | 1 | __________________________________________________________________________|____________| Prostate | | 49 | Leukemia Mononuclear | | 7 | __________________________________________________________________________|____________| Seminal Vesicle | | 49 | Leukemia Mononuclear | | 3 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 92 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 10000PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ GENITAL SYSTEM - cont | | | | | | __________________________________________________________________________|____________| Testes | | 50 | Leukemia Mononuclear | | 7 | Bilateral, Interstitial Cell, Adenoma| | 33 | Interstitial Cell, Adenoma | | 11 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | | | | __________________________________________________________________________|____________| Bone Marrow | | 50 | Histiocytic Sarcoma | | 2 | Leukemia Mononuclear | | 22 | __________________________________________________________________________|____________| Lymph Node | | 18 | Leukemia Mononuclear | | 1 | Bronchial, Leukemia Mononuclear | | 1 | Iliac, Leukemia Mononuclear | | 1 | Inguinal, Leukemia Mononuclear | | 1 | Lumbar, Leukemia Mononuclear | | 2 | Mediastinal, Leukemia Mononuclear | | 5 | Pancreatic, Leukemia Mononuclear | | 5 | Renal, Leukemia Mononuclear | | 1 | __________________________________________________________________________|____________| Lymph Node, Mandibular | | 50 | Leukemia Mononuclear | | 18 | __________________________________________________________________________|____________| Lymph Node, Mesenteric | | 50 | Leukemia Mononuclear | | 17 | __________________________________________________________________________|____________| Spleen | | 49 | Leukemia Mononuclear | | 25 | Sarcoma | | 1 | __________________________________________________________________________|____________| Thymus | | 48 | Leukemia Mononuclear | | 16 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | | | | __________________________________________________________________________|____________| Mammary Gland | | 46 | Adenocarcinoma | | 1 | Fibroadenoma | | 1 | Histiocytic Sarcoma | | 1 | Leukemia Mononuclear | | 4 | __________________________________________________________________________|____________| Skin | | 50 | Keratoacanthoma | | 3 | Leukemia Mononuclear | | 4 | Squamous Cell Papilloma | | 1 | Sebaceous Gland, Adenoma | | 1 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 93 NTP Experiment-Test: 05130-03 NEOPLASMS BY INDIVIDUAL ANIMAL Report: PEIRPT04 Study Type: CHRONIC C. 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DIRECT BLUE 218 Date: 04/03/97 Route: DOSED FEED Time: 12:13:37 __________________________________________________________________________________________________________________________________ | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | | DAY ON TEST | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4| | | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7| | | _____________________________________________________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | O | FISCHER 344 RATS MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| | T | ANIMAL ID | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| | A | 10000PPM | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| | L | | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| | | __________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM - cont | | | | | | Subcutaneous Tissue, Fibroma | | 2 | Subcutaneous Tissue, Fibrosarcoma | | 1 | Subcutaneous Tissue, Histiocytic | | | Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | | | | __________________________________________________________________________|____________| Bone | | 50 | __________________________________________________________________________|____________| Skeletal Muscle | | 1 | Histiocytic Sarcoma | | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | | | | __________________________________________________________________________|____________| Brain | | 50 | Leukemia Mononuclear | | 6 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | | | | __________________________________________________________________________|____________| Lung | | 50 | Alveolar/Bronchiolar Adenoma | | 2 | Histiocytic Sarcoma | | 1 | Leukemia Mononuclear | | 23 | __________________________________________________________________________|____________| Nose | | 50 | Leukemia Mononuclear | | 8 | __________________________________________________________________________|____________| Trachea | | 50 | Leukemia Mononuclear | | 4 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | | | | __________________________________________________________________________|____________| Eye | | 2 | __________________________________________________________________________|____________| Zymbal's Gland | | 48 | Carcinoma | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | | | | __________________________________________________________________________|____________| Kidney | | 50 | Leukemia Mononuclear | | 23 | __________________________________________________________________________|____________| Urethra | | 1 | __________________________________________________________________________|____________| Urinary Bladder | | 50 | Leukemia Mononuclear | | 8 | __________________________________________________________________________________________________________________________________ SYSTEMIC LESIONS | | | __________________________________________________________________________|____________| Multiple Organs | | 50 | Histiocytic Sarcoma | | 2 | Leukemia Mononuclear | | 26 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with tumor + : Tissue examined microscopically M : Missing tissue X : Lesion present A : Autolysis precludes evaluation I : Insufficient tissue BLANK : Not examined microscopically Page 94 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------