TDMS Study 05133-04 Pathology Tables
INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
ACETONITRILE
NTP Experiment-Test: 05133-04 Report: PEIRPT03
Study Type: CHRONIC Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
Facility: Battelle Northwest
Chemical CAS #: 75-05-8
Lock Date: 07/17/92
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 60 60 60
Scheduled Sacrifice 10 10 10 10
Early Deaths
Natural Death 6 5 8 5
Moribund Sacrifice 15 12 13 13
Accidently Killed 1
Survivors
Natural Death 1
Terminal Sacrifice 28 32 29 32
Animals Examined Microscopically 60 60 60 60
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (52) (55) (51) (56)
Hyperplasia 1 (2%)
Inflammation, Suppurative 2 (4%) 1 (2%) 1 (2%)
Intestine Large, Colon (56) (55) (57) (58)
Hemorrhage 1 (2%)
Necrosis 1 (2%)
Intestine Large, Cecum (55) (56) (58) (56)
Hemorrhage 1 (2%)
Necrosis 1 (2%)
Intestine Small, Duodenum (52) (54) (54) (55)
Inflammation, Suppurative 1 (2%)
Intestine Small, Jejunum (54) (56) (56) (57)
Peyer's Patch, Hyperplasia 1 (2%)
Intestine Small, Ileum (55) (57) (55) (57)
Amyloid Deposition 1 (2%)
Liver (59) (60) (60) (59)
Angiectasis 1 (2%)
Basophilic Focus 2 (3%) 3 (5%)
Clear Cell Focus 1 (2%) 1 (2%)
Cyst 1 (2%)
Cytomegaly 1 (2%)
Degeneration, Cystic 1 (2%)
Degeneration, Fatty 2 (3%) 1 (2%)
Eosinophilic Focus 5 (8%) 3 (5%) 3 (5%) 4 (7%)
Focal Cellular Change 1 (2%) 2 (3%)
Hematopoietic Cell Proliferation 8 (14%) 2 (3%) 1 (2%) 4 (7%)
Hyperplasia, Lymphoid 1 (2%)
Infarct 1 (2%) 1 (2%)
Infiltration Cellular, Mononuclear Cell 2 (3%)
Karyomegaly 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 2
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Mitotic Alteration 2 (3%) 1 (2%)
Mixed Cell Focus 4 (7%) 4 (7%) 1 (2%)
Necrosis 5 (8%) 4 (7%) 8 (13%) 2 (3%)
Pigmentation, Hemosiderin 1 (2%)
Regeneration 1 (2%)
Bile Duct, Cyst 1 (2%) 1 (2%)
Bile Duct, Degeneration 1 (2%)
Centrilobular, Necrosis 1 (2%) 1 (2%) 3 (5%)
Serosa, Inflammation, Suppurative 2 (3%)
Mesentery (10) (16) (7) (5)
Angiectasis 1 (10%)
Inflammation, Chronic 2 (20%)
Fat, Necrosis 7 (70%) 9 (56%) 3 (43%) 4 (80%)
Pancreas (59) (59) (60) (58)
Amyloid Deposition 1 (2%)
Atrophy 2 (3%) 2 (3%) 1 (2%)
Focal Cellular Change 2 (3%) 1 (2%) 2 (3%)
Hyperplasia 1 (2%) 1 (2%)
Hyperplasia, Lymphoid 1 (2%)
Inflammation, Chronic 3 (5%) 1 (2%) 1 (2%)
Duct, Ectasia 3 (5%)
Duct, Inflammation, Chronic 2 (3%)
Salivary Glands (59) (60) (60) (59)
Inflammation, Chronic 1 (2%)
Inflammation, Suppurative 1 (2%)
Stomach, Forestomach (59) (60) (60) (58)
Angiectasis 1 (2%)
Diverticulum 1 (2%)
Hyperkeratosis 5 (9%)
Hyperplasia, Squamous 2 (3%) 8 (13%) 9 (15%) 25 (43%)
Inflammation, Suppurative 1 (2%) 3 (5%) 4 (7%) 3 (5%)
Necrosis 1 (2%)
Ulcer 1 (2%) 2 (3%) 2 (3%) 5 (9%)
Epithelium, Hyperplasia 1 (2%)
Stomach, Glandular (59) (58) (60) (58)
Hyperplasia 2 (3%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Mineralization 1 (2%)
Tooth (1)
Dysplasia 1 (100%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Blood Vessel (52) (53) (55) (55)
Mineralization 1 (2%) 1 (2%)
Thrombosis 1 (2%)
Heart (59) (60) (59) (60)
Angiectasis 1 (2%)
Cardiomyopathy 16 (27%) 19 (32%) 23 (39%) 15 (25%)
Hyperplasia, Atypical 1 (2%) 1 (2%)
Inflammation, Suppurative 1 (2%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (59) (59) (60) (59)
Cyst 3 (5%) 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 2 (3%) 2 (3%) 1 (2%)
Hyperplasia 4 (7%) 2 (3%)
Hypertrophy 3 (5%) 4 (7%) 7 (12%) 3 (5%)
Capsule, Hyperplasia 1 (2%) 1 (2%)
Adrenal Medulla (58) (58) (60) (58)
Hyperplasia 2 (3%) 2 (3%) 3 (5%) 2 (3%)
Islets, Pancreatic (59) (59) (60) (57)
Hyperplasia 2 (3%) 5 (8%) 1 (2%)
Pituitary Gland (58) (59) (60) (58)
Pars Distalis, Cyst 4 (7%) 3 (5%) 1 (2%) 1 (2%)
Pars Distalis, Hyperplasia 19 (33%) 16 (27%) 24 (40%) 21 (36%)
Pars Distalis, Inflammation, Chronic 1 (2%)
Pars Intermedia, Hyperplasia 1 (2%)
Thyroid Gland (59) (58) (60) (59)
Inflammation 1 (2%)
Inflammation, Suppurative 1 (2%)
Follicle, Cyst 1 (2%) 1 (2%) 3 (5%)
Follicular Cell, Hyperplasia 12 (20%) 6 (10%) 9 (15%) 6 (10%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Clitoral Gland (40) (41) (46) (44)
Atrophy 1 (3%)
Ovary (58) (57) (58) (57)
Abscess 4 (7%)
Amyloid Deposition 1 (2%) 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Angiectasis 3 (5%) 1 (2%) 2 (3%)
Atrophy 17 (29%) 19 (33%) 20 (34%) 13 (23%)
Cyst 19 (33%) 18 (32%) 18 (31%) 13 (23%)
Mineralization 1 (2%)
Germinal Epithelium, Hyperplasia 1 (2%)
Interstitium, Hyperplasia 1 (2%)
Uterus (59) (59) (60) (59)
Amyloid Deposition 1 (2%)
Angiectasis 1 (2%) 2 (3%)
Dilatation 2 (3%) 1 (2%)
Hyperplasia, Cystic 57 (97%) 53 (90%) 55 (92%) 52 (88%)
Inflammation, Suppurative 1 (2%) 2 (3%) 1 (2%)
Thrombosis 1 (2%)
Arteriole, Hypertrophy 1 (2%)
Artery, Inflammation, Chronic 1 (2%)
Myometrium, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (59) (59) (60) (59)
Hyperplasia, Megakaryocyte 1 (2%)
Hyperplasia, Neutrophil 6 (10%) 1 (2%) 3 (5%) 3 (5%)
Lymph Node (7) (9) (9) (1)
Axillary, Hyperplasia 1 (11%)
Iliac, Hemorrhage 1 (11%)
Iliac, Hyperplasia 2 (29%) 1 (11%) 1 (11%)
Popliteal, Hyperplasia 1 (11%)
Renal, Angiectasis 1 (11%)
Renal, Congestion 1 (11%)
Lymph Node, Bronchial (48) (54) (51) (46)
Erythrophagocytosis 1 (2%)
Hematopoietic Cell Proliferation 2 (4%)
Hyperplasia 11 (23%) 4 (7%) 7 (14%) 4 (9%)
Lymph Node, Mandibular (42) (45) (52) (41)
Hyperplasia 5 (12%) 4 (8%) 5 (12%)
Lymph Node, Mesenteric (54) (53) (57) (51)
Amyloid Deposition 1 (2%)
Angiectasis 1 (2%) 1 (2%) 2 (4%)
Congestion 2 (4%) 1 (2%)
Hematopoietic Cell Proliferation 1 (2%)
Hemorrhage 1 (2%)
Hyperplasia 3 (6%) 5 (9%) 9 (16%) 4 (8%)
Hyperplasia, Histiocytic 1 (2%)
Inflammation, Chronic 1 (2%)
Lymph Node, Mediastinal (43) (43) (44) (41)
Erythrophagocytosis 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - CONT
Hematopoietic Cell Proliferation 2 (5%) 1 (2%)
Hyperplasia 6 (14%) 1 (2%) 10 (23%) 5 (12%)
Inflammation, Suppurative 2 (5%)
Spleen (59) (59) (60) (58)
Amyloid Deposition 1 (2%) 1 (2%)
Congestion 1 (2%)
Fibrosis 1 (2%)
Hematopoietic Cell Proliferation 12 (20%) 12 (20%) 15 (25%) 11 (19%)
Hemorrhage 1 (2%)
Hyperplasia, Lymphoid 14 (24%) 15 (25%) 13 (22%) 5 (9%)
Capsule, Fibrosis 1 (2%)
Thymus (54) (58) (56) (51)
Angiectasis 1 (2%) 2 (4%) 1 (2%)
Atrophy 5 (9%) 2 (3%) 1 (2%) 5 (10%)
Developmental Malformation 1 (2%)
Hyperplasia, Lymphoid 6 (11%) 5 (9%) 12 (21%) 8 (16%)
Necrosis 1 (2%) 1 (2%)
Epithelial Cell, Hyperplasia 2 (4%) 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (59) (57) (60) (57)
Hyperplasia 1 (2%) 2 (4%) 2 (3%) 1 (2%)
Skin (59) (60) (60) (59)
Inflammation, Suppurative 1 (2%) 2 (3%)
Sebaceous Gland, Hyperplasia 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic 1 (2%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (59) (60) (60) (60)
Fibrous Osteodystrophy 27 (46%) 18 (30%) 16 (27%) 19 (32%)
Fracture 1 (2%)
Inflammation, Chronic 1 (2%)
Skeletal Muscle (1) (1) (1)
Hemorrhage 1 (100%) 1 (100%)
Inflammation 1 (100%) 1 (100%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (59) (60) (60) (59)
Hemorrhage 1 (2%)
Inflammation 1 (2%)
Inflammation, Chronic 1 (2%) 1 (2%) 3 (5%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM - CONT
Mineralization 28 (47%) 31 (52%) 28 (47%) 20 (34%)
Necrosis 1 (2%)
Pigmentation, Hemosiderin 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (59) (60) (60) (59)
Embolus 1 (2%)
Hemorrhage 4 (7%) 1 (2%) 5 (8%) 4 (7%)
Infiltration Cellular, Mononuclear Cell 1 (2%)
Inflammation, Acute 1 (2%) 1 (2%)
Inflammation, Suppurative 1 (2%)
Thrombosis 1 (2%) 2 (3%)
Alveolar Epithelium, Hyperplasia 3 (5%) 4 (7%) 2 (3%) 1 (2%)
Alveolus, Infiltration Cellular, Histiocyte 1 (2%) 3 (5%) 2 (3%) 1 (2%)
Arteriole, Inflammation 2 (3%)
Artery, Mediastinum, Inflammation 1 (2%)
Mediastinum, Hemorrhage 1 (2%)
Mediastinum, Inflammation, Suppurative 1 (2%)
Perivascular, Infiltration Cellular,
Mononuclear Cell 2 (3%) 3 (5%) 1 (2%)
Nose (59) (60) (60) (59)
Inflammation, Suppurative 1 (2%) 2 (3%)
Nasolacrimal Duct, Inflammation, Suppurative 1 (2%) 2 (3%) 1 (2%)
Olfactory Epithelium, Atrophy 5 (8%) 4 (7%) 5 (8%) 2 (3%)
Olfactory Epithelium, Metaplasia 4 (7%) 3 (5%) 2 (3%) 1 (2%)
Olfactory Epithelium, Necrosis 1 (2%) 1 (2%)
Respiratory Epithelium, Hyperplasia 1 (2%)
Respiratory Epithelium, Metaplasia, Squamous 1 (2%)
Respiratory Epithelium, Necrosis 1 (2%) 1 (2%)
Trachea (59) (59) (59) (59)
Metaplasia, Squamous 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (59) (59) (60) (58)
Amyloid Deposition 1 (2%) 2 (3%)
Hydronephrosis 1 (2%)
Hyperplasia, Atypical 1 (2%)
Infarct 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Infiltration Cellular, Mononuclear Cell 1 (2%)
Inflammation, Suppurative 1 (2%)
Metaplasia, Osseous 3 (5%) 1 (2%) 3 (5%)
Nephropathy 24 (41%) 32 (54%) 27 (45%) 25 (43%)
Arteriole, Inflammation, Chronic 1 (2%)
Cortex, Cyst 1 (2%)
Cortex, Pigmentation, Hemosiderin 1 (2%) 1 (2%)
Glomerulus, Embolus 1 (2%)
Renal Tubule, Mineralization 1 (2%)
Renal Tubule, Necrosis 2 (3%)
Urinary Bladder (57) (57) (59) (57)
Inflammation, Chronic 1 (2%) 2 (3%)
Ulcer 1 (2%)
Arteriole, Inflammation 1 (2%)
Transitional Epithelium, Hyperplasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 8
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 60 60 60 60
Scheduled Sacrifice 10 10 10 10
Early Deaths
Moribund Sacrifice 13 14 16 6
Natural Death 5 4 2 1
Survivors
Terminal Sacrifice 32 32 32 43
Animals Examined Microscopically 60 60 60 60
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Gallbladder (54) (56) (55) (55)
Cyst 1 (2%)
Degeneration 2 (4%) 1 (2%) 1 (2%)
Hyperplasia 1 (2%)
Inflammation, Suppurative 3 (5%) 1 (2%) 3 (5%)
Intestine Large, Cecum (57) (59) (58) (58)
Necrosis 1 (2%)
Intestine Small, Duodenum (56) (58) (58) (58)
Peyer's Patch, Hyperplasia 1 (2%)
Intestine Small, Jejunum (55) (58) (57) (59)
Peyer's Patch, Hyperplasia 1 (2%) 1 (2%) 1 (2%) 1 (2%)
Intestine Small, Ileum (56) (59) (58) (59)
Peyer's Patch, Hyperplasia 1 (2%) 1 (2%)
Liver (60) (60) (59) (60)
Angiectasis 1 (2%) 1 (2%) 1 (2%)
Basophilic Focus 1 (2%) 2 (3%) 5 (8%) 1 (2%)
Clear Cell Focus 4 (7%) 1 (2%) 2 (3%) 3 (5%)
Cytomegaly 1 (2%)
Degeneration, Fatty 5 (8%) 9 (15%) 12 (20%) 4 (7%)
Eosinophilic Focus 5 (8%) 5 (8%) 5 (8%) 4 (7%)
Focal Cellular Change 1 (2%) 1 (2%)
Hematopoietic Cell Proliferation 2 (3%) 3 (5%) 4 (7%) 2 (3%)
Hemorrhage 1 (2%) 1 (2%)
Infarct 2 (3%) 2 (3%) 2 (3%) 1 (2%)
Karyomegaly 4 (7%) 3 (5%) 2 (3%) 3 (5%)
Mitotic Alteration 1 (2%) 3 (5%)
Mixed Cell Focus 2 (3%) 6 (10%) 3 (5%) 3 (5%)
Necrosis 5 (8%) 4 (7%) 1 (2%) 1 (2%)
Vacuolization Cytoplasmic 1 (2%)
Bile Duct, Cyst 3 (5%) 1 (2%)
Bile Duct, Degeneration 1 (2%)
Bile Duct, Hyperplasia 1 (2%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 9
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - CONT
Centrilobular, Necrosis 2 (3%) 2 (3%)
Kupffer Cell, Pigmentation 1 (2%)
Mesentery (3) (3) (3) (3)
Angiectasis 1 (33%)
Inflammation, Chronic 1 (33%)
Fat, Necrosis 2 (67%) 1 (33%) 2 (67%) 2 (67%)
Pancreas (59) (60) (58) (60)
Atrophy 1 (2%) 1 (2%) 3 (5%)
Focal Cellular Change 3 (5%) 2 (3%) 1 (2%)
Inflammation, Chronic 2 (3%)
Duct, Ectasia 1 (2%)
Salivary Glands (60) (60) (58) (60)
Cyst 1 (2%)
Inflammation, Chronic 1 (2%)
Inflammation, Suppurative 1 (2%)
Stomach, Forestomach (59) (60) (58) (60)
Hyperkeratosis 1 (2%) 3 (5%) 2 (3%)
Hyperplasia, Squamous 3 (5%) 5 (8%) 6 (10%) 15 (25%)
Inflammation, Suppurative 2 (3%) 1 (2%) 4 (7%) 2 (3%)
Ulcer 1 (2%) 1 (2%) 1 (2%) 2 (3%)
Stomach, Glandular (59) (60) (58) (60)
Degeneration 1 (2%) 1 (2%)
Ectopic Tissue 1 (2%)
Erosion 2 (3%) 1 (2%)
Hyperplasia 1 (2%) 2 (3%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Pigmentation, Hemosiderin 2 (3%) 1 (2%) 1 (2%)
Tooth (3) (1)
Dysplasia 2 (67%)
Inflammation 1 (33%) 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (60) (60) (59) (60)
Angiectasis 1 (2%)
Cardiomyopathy 40 (67%) 42 (70%) 46 (78%) 44 (73%)
Necrosis 1 (2%) 1 (2%)
Arteriole, Inflammation, Chronic 2 (3%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (58) (60) (58) (60)
Accessory Adrenal Cortical Nodule 1 (2%) 1 (2%) 1 (2%)
Cyst 1 (2%) 1 (2%)
Hyperplasia 7 (12%) 11 (18%) 13 (22%) 12 (20%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 10
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM - CONT
Hypertrophy 28 (48%) 36 (60%) 23 (40%) 37 (62%)
Capsule, Hyperplasia 1 (2%)
Adrenal Medulla (59) (60) (57) (60)
Hyperplasia 2 (3%) 1 (2%) 1 (2%)
Islets, Pancreatic (57) (60) (58) (60)
Hyperplasia 6 (11%) 7 (12%) 9 (16%) 4 (7%)
Parathyroid Gland (46) (44) (49) (35)
Hyperplasia 1 (2%)
Pituitary Gland (56) (58) (56) (59)
Pars Distalis, Cyst 4 (7%) 2 (3%) 1 (2%) 3 (5%)
Pars Distalis, Hyperplasia 1 (2%) 3 (5%) 2 (4%)
Thyroid Gland (59) (60) (58) (60)
Follicle, Cyst 1 (2%) 2 (3%)
Follicular Cell, Hyperplasia 7 (12%) 5 (8%) 2 (3%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (60) (60) (58) (60)
Granuloma Sperm 1 (2%) 2 (3%)
Hyperplasia 1 (2%)
Inflammation, Chronic Active 1 (2%)
Inflammation, Suppurative 1 (2%)
Preputial Gland (59) (60) (56) (60)
Atrophy 2 (3%) 1 (2%)
Ectasia 4 (7%) 12 (20%) 4 (7%) 2 (3%)
Inflammation, Chronic Active 14 (24%) 16 (27%) 9 (16%) 20 (33%)
Prostate (54) (53) (52) (54)
Inflammation, Suppurative 1 (2%) 1 (2%) 1 (2%) 2 (4%)
Seminal Vesicle (59) (60) (57) (60)
Inflammation, Chronic Active 1 (2%) 1 (2%) 1 (2%)
Inflammation, Suppurative 1 (2%) 1 (2%)
Testes (60) (60) (59) (60)
Atrophy 3 (5%) 1 (2%) 1 (2%) 2 (3%)
Mineralization 1 (2%)
Interstitial Cell, Hyperplasia 1 (2%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 11
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (59) (60) (58) (60)
Angiectasis 1 (2%) 1 (2%)
Fibrosis 2 (3%)
Hyperplasia, Megakaryocyte 1 (2%)
Hyperplasia, Neutrophil 5 (8%) 7 (12%) 9 (16%) 11 (18%)
Thrombosis 1 (2%)
Lymph Node (3) (2) (2)
Iliac, Hyperplasia 1 (50%)
Pancreatic, Hyperplasia 1 (50%)
Renal, Hyperplasia 1 (50%)
Lymph Node, Bronchial (41) (44) (45) (50)
Hyperplasia 1 (2%) 2 (5%)
Lymph Node, Mandibular (43) (39) (36) (44)
Hyperplasia 2 (5%) 3 (8%) 1 (3%) 2 (5%)
Lymph Node, Mesenteric (56) (59) (57) (58)
Angiectasis 1 (2%)
Congestion 2 (3%) 5 (9%) 1 (2%)
Hematopoietic Cell Proliferation 2 (4%) 3 (5%)
Hyperplasia 5 (9%) 10 (17%) 8 (14%) 3 (5%)
Lymph Node, Mediastinal (47) (45) (44) (40)
Hyperplasia 2 (4%) 2 (4%) 1 (2%)
Spleen (59) (60) (58) (60)
Amyloid Deposition 1 (2%)
Angiectasis 1 (2%)
Hematopoietic Cell Proliferation 12 (20%) 15 (25%) 16 (28%) 13 (22%)
Hyperplasia, Lymphoid 4 (7%) 2 (3%) 4 (7%) 8 (13%)
Hyperplasia, Mononuclear Cell 1 (2%)
Thymus (53) (54) (48) (57)
Atrophy 4 (8%) 2 (4%) 1 (2%) 1 (2%)
Hyperplasia, Lymphoid 1 (2%) 1 (2%)
Necrosis 1 (2%)
Epithelial Cell, Hyperplasia 1 (2%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (60) (59) (59) (60)
Cyst Epithelial Inclusion 1 (2%)
Inflammation, Suppurative 1 (2%) 2 (3%)
Pinna, Granuloma 1 (2%)
Subcutaneous Tissue, Inflammation, Chronic 6 (10%) 8 (14%) 10 (17%) 10 (17%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 12
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (60) (60) (59) (60)
Fibrous Osteodystrophy 1 (2%)
Inflammation, Suppurative 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (60) (60) (58) (60)
Mineralization 23 (38%) 26 (43%) 16 (28%) 20 (33%)
Thrombosis 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Larynx (59) (60) (58) (60)
Inflammation, Suppurative 1 (2%)
Lung (60) (60) (58) (60)
Hemorrhage 5 (8%) 4 (7%) 1 (2%) 1 (2%)
Inflammation, Chronic, Focal 1 (2%)
Inflammation, Granulomatous 1 (2%)
Alveolar Epithelium, Hyperplasia 4 (7%) 5 (8%) 3 (5%) 4 (7%)
Perivascular, Infiltration Cellular,
Mononuclear Cell 1 (2%)
Nose (60) (60) (58) (60)
Exudate 1 (2%)
Inflammation, Suppurative 3 (5%) 2 (3%) 3 (5%) 2 (3%)
Nasolacrimal Duct, Inflammation, Suppurative 4 (7%) 2 (3%)
Olfactory Epithelium, Atrophy 4 (7%) 1 (2%) 2 (3%) 1 (2%)
Olfactory Epithelium, Metaplasia 3 (5%) 1 (2%) 2 (3%) 1 (2%)
Respiratory Epithelium, Hyperplasia 1 (2%)
Respiratory Epithelium, Metaplasia, Squamous 1 (2%)
Trachea (59) (60) (58) (60)
Metaplasia, Squamous 1 (2%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (59) (60) (58) (60)
Amyloid Deposition 1 (2%)
Hydronephrosis 3 (5%) 5 (8%) 4 (7%) 6 (10%)
Hyperplasia, Cystic 2 (3%) 1 (2%) 2 (3%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 13
NTP Experiment-Test: 05133-04 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: CHRONIC ACETONITRILE Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 06:55:05
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE CONTROL 50 PPM 100 PPM 200 PPM
____________________________________________________________________________________________________________________________________
URINARY SYSTEM - CONT
Inflammation, Suppurative 1 (2%) 3 (5%) 3 (5%) 2 (3%)
Metaplasia, Osseous 1 (2%) 1 (2%)
Nephropathy 41 (69%) 41 (68%) 39 (67%) 43 (72%)
Arteriole, Inflammation, Chronic 1 (2%)
Cortex, Cyst 5 (8%) 2 (3%) 3 (5%) 1 (2%)
Renal Tubule, Hyperplasia 1 (2%)
Renal Tubule, Mineralization 1 (2%)
Urinary Bladder (59) (58) (59) (60)
Dilatation 2 (3%) 2 (3%) 3 (5%)
Hemorrhage 1 (2%)
Inflammation, Chronic 1 (2%) 1 (2%) 3 (5%) 1 (2%)
Inflammation, Suppurative 2 (3%) 1 (2%)
Pigmentation, Melanin 1 (2%)
Ulcer 1 (2%) 1 (2%) 1 (2%)
Transitional Epithelium, Hyperplasia 2 (3%) 1 (2%) 2 (3%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 14
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